Skip to main navigation menu Skip to main content Skip to site footer
Plant Science Today (PST)

Research Articles

Vol. 12 No. 1 (2025)

Influence of seed priming to alleviate the seed dormancy and growth performance in wild moth bean (Vigna aconitifolia (Jacq.) Marechal)

DOI
https://doi.org/10.14719/pst.6261
Submitted
22 November 2024
Published
02-03-2025 — Updated on 18-03-2025
Versions

Abstract

To determine the most effective techniques for breaking moth bean seed dormancy and evaluating the impact of seed priming on growth performance and storage potential, experiments were conducted in 2022–23 at the Agricultural College and Research Institute, Eachangkottai, Thanjavur. The study examined various treatments for reducing seed dormancy and enhancing growth performance in wild moth beans. Freshly harvested wild moth bean seeds were subjected to seven different seed priming methods: soaking in water for 4 hours and 6 hours; soaking in gibberellic acid (GA3) at 100 ppm for 30 min. and 60 min; and soaking in potassium nitrate (0.02 M) for 30 min. Compared against untreated control. The results revealed that seeds soaked in GA3 (100 ppm) showed significantly higher germination and superior seedling growth parameters than the other treatments.
Regarding the soaking durations, seeds soaked in GA3 (100 ppm) for 60 min recorded higher seed germination and seedling vigour. Seed quality measures were assessed at trimonthly intervals while dormancy broken seeds were kept in Thanjavur's ambient settings for 9 months. As per the results of the storage experiment, wild moth bean seeds were kept for 9 months without significantly reducing their germination rate. Further, a linear reduction was observed in seedling length. A significant increase was recorded in moisture, EC, dehydrogenase, alpha-amylase, and superoxide dismutase. Current research concluded that wild moth bean seeds were treated with GA3 (100 ppm for 60 min) as a seed priming procedure to break dormancy and preserve seed vigour for up to nine months of storage.

References

  1. Gayacharan CKR, Chandora R, Rana JC. Moth bean (Vigna aconitifolia): a minor legume with major potential to address global agricultural challenges. Front Plant Sci. 2023;6(14):1179547. https://doi: 10.3389/fpls.2023.1179547.
  2. Abdul-Baki AA, Anderson JD. Vigour determination in soybean by multiple criteria. Crop Sci. 1973;13:630?33. https://doi.org/10.2135/cropsci1973.0011183X001300060013x
  3. ISTA. International rules for seed testing, vol 1. Bassersdorf: International Seed Testing Association; 2021 https://doi.org/10.15258/istarules.2021
  4. Anonymous. International rules for seed testing. Seed Sci and Technol. 1999;13(2):356–62. https://doi.org/10.4236/oalib.1105375
  5. Presley JT. Relations of protoplast permeability to cotton seed viability and predisposition to seedling disease. Plant Disease Reporter. 1958;42:852. https://doi.org/10.25081/jp.2019.v11.5613
  6. Bradford M. A rapid and sensitive method for quantitating microgram quantities of proteins utilizing the principle of protein dye-binding. Anal Bioche. 1976;72:248–54. https://doi.org/10.1016/0003-2697(76)90527-3
  7. Kittock DL, Law AG. Relationship of seedling vigour to respiration and tetrazolium reduction in germinating wheat seeds. Agron J. 1968;60:268–88. https://doi.org/10.2134/agronj1968.00021962006000030012x
  8. Dhindsa RS, Plumb-Dhindsa SP, Reid DM. Leaf senescence and lipid peroxidation. Effects of some phytohormones and scavengers of free radicals and singlet oxygen. Physiol Plant. 1982;6:457. http://dx.doi.org/10.1111/j.1399-3054.1982.tb04539.x
  9. Panse VG, Sukhatma PV. Statistical methods for agricultural workers. 2nd edition. New Delhi: Indian Council of Agricultural Research; 1985 https://doi.org/10.4236/tel.2018.810121
  10. Jones R, Varner J. The bioassay of gibberellins. Planta. 1967;72:155–61. https://doi.org/10.1007/bf00387479
  11. Bradford KJ. Manipulation of seed water relations via osmatic priming to improve germination under stress conditions. Hort Sci. 1986;21:1105–12. https://doi.org/10.21273/hortsci.21.5.1105
  12. de Castro RD, Zheng X, Bergervoet JH, De Vos C, Bino RJ. Dergi hakk?nda, uluslararas? tar?m ara?t?rmalar?nda yenilikçiyakla??mlar dergisi. Int J Innov App Agric Res. 2022;6(4):340–50. https://doi.org/10.29329/ijiaar.2024.1075.3
  13. Job D, Job CI, Dacher C, Corbineau F, Come D. Identification of germination-specific protein markers and their use in seed priming technology. In: Black -Bradford M, Vaavez-Ramos KJ, editors. Seed biology: advances and applications. Wallingford UK: CABI; 1999. p. 449–59. http://dx.doi.org/10.1079/9780851994048.0449
  14. Varier A, Vari AK, Dadlanium. The subcellular basis of seed priming. Curr Sci. 2010;99:450–56.
  15. Ann-Evans S, Mitchell RJ, Cabin RJ. Morphological side effects of using gibberellic acid to induce germination: Consequences for the study of seed dormancy. Am J Bot. 1996;83(5):543–49. https://doi.org/10.2307/2445911
  16. Azadi MS, Tabatabaei SA, Younesi E, Rostami MR, Mombeni M. Hormone priming improves germination characteristics and enzyme activity of sorghum seeds (Sorghum bicolor L.) under accelerated aging. Cercetari Agronomice in Moldova. 2013;46:49?55. http://dx.doi.org/10.2478/v10298-012-0092-8
  17. Kandil AA, Sharief AE, Abido WAE, Awed AM. Effect of gibberellic acid on germination behaviour of sugar beet cultivars under salt stress conditions of Egypt. Sugar Tech. 2014;16(2):211–21. http://dx.doi.org/10.1007/s12355-013-0252-7s
  18. Rouhi HR, Sepehri A. Hormonal priming to overcome drought stress and aging damage in groundnut seed (Arachis hypogaea L.). Iran J Plant Physiol. 2020;10(2):3167–74. https://doi.org/10.3390/agronomy12061288
  19. Zhu ZH, Sami A, Xu QQ, Wu LL , Zheng WY, Chen ZP, Zhou KJ. Effects of seed priming treatments on the germination and development of two rapeseed (Brassica napus L.) varieties under the co-influence of low temperature and drought. Plos One. 2021;16(9):e0257236. https://doi.org/10.1371/journal.pone.0257236
  20. Tsegay BA, Andargie M. Seed priming with gibberellic acid (GA3) alleviates salinity-induced inhibition of germination and seedling growth of Zea mays L., Pisum sativum var. abyssinicum A. Braun and Lathyrus sativus L. J Crop Sci Biotech. 2018;21(3):261–67. http://dx.doi.org/10.1007/s12892-018-0043-0
  21. McDonough CM, Floyd CD, Waniska RD, Rooney LW. Effect of accelerated aging on maize, sorghum and sorghum meal. J Cereal Sci. 2004;39:351–61. https://doi.org/10.1016/j.jcs.2004.01.001
  22. Khajeh M, Tabatabaei SA, Ansari O, Zadeh SF. Improvement of germination characteristics and enhancement of antioxidant enzymes activity of safflower (Carthamus tinctorius L.) aged seeds by use of gibberellin. Cercetãri Agronomiceîn Moldova. 2015;48(3):33–41. http://dx.doi.org/10.1515/cerce-2015-0039
  23. Rame G. Studies on seed senescence and Seed vigour in some genotypes of rice (Oryza sativa L.). PhD [thesis]. Coimbatore: Tamil Nadu Agricultural University; 1992

Downloads

Download data is not yet available.