Skip to main navigation menu Skip to main content Skip to site footer
Plant Science Today (PST)

Research Articles

Vol. 12 No. 1 (2025)

Optimization of gamma irradiation doses (60Co) for mutagenesis in strawberry (Fragaria × annanasa Duch) cv. Winter Dawn

DOI
https://doi.org/10.14719/pst.6270
Submitted
22 November 2024
Published
23-01-2025 — Updated on 04-02-2025
Versions

Abstract

Strawberry, a vegetatively propagated crop, exhibits limited genetic variation, which constrains its adaptability and improvement potential. Gamma-ray radiation is a promising approach for enhancing genetic diversity and improving strawberries. Strawberry runners of the Winter Dawn cultivar were irradiated using 60Co, with doses ranging from 20 Gy to 40 Gy administered at a consistent rate of 1.52 Gy per minute to evaluate mutagenic effects. The survival effects in gamma irradiation-treated runners were recorded at 30 days after transplantation. An LD50 analysis was conducted to assess physiological impacts, and the lethal dose value was found to be 40 Gy, resulting in 50% mortality. Distinct differences in survival rates were observed between treated and untreated runners, with the highest survival rate (88%) at 20 Gy. The 30 Gy dose-treated runners exhibited the highest growth parameters, including crown diameter (14.32 mm), primary root length (29.50 cm), secondary root length (5.5 cm), and number of roots (39.6). Among fruit quality traits, maximum fruit length (39.84 mm), and fruit diameter (27.80 mm) were observed in runners treated with a 30 Gy gamma irradiation dose. Significant variations in survival, growth, and fruit quality were observed across different gamma irradiation doses. These findings provide a foundation for future efforts to develop potential strawberry mutants with improved traits through gamma irradiation. These findings provide a foundation for future efforts to develop potential strawberry mutants with improved traits through gamma irradiation.

References

  1. Liston A, Cronn R, Ashman TL. Fragaria a genus with deep historical roots and ripe for evolutionary and ecological insights. Am J Bot. 2014;101(10):1686-1699. https://doi.org/10.3732/ajb.1400140
  2. Qarni A, Muhammad K, Wahab A, Ali A, Khizar C, Ullah I. Molecular characterization of wild and cultivated strawberry (Fragaria × ananassa) through DNA barcode markers. Genetics Res.2022;2022:9249561. https://doi.org/10.1155/2022/9249561
  3. Nunes CF, Ferreira JL, Generoso AL, Dias MSC, Pasqual M, Cançado GMA. The genetic diversity of strawberry (Fragaria ananassa Duch.) hybrids based on ISSR markers. Acta Sci Agron. 2013;(35):443-452. https://doi.org/10.4025/actasciagron.v35i4.16737
  4. Bado S, Forster BP, Nielen S, Ali AM, Lagoda PJL, Till BJ, et al. Plant mutation breeding: current progress and future assessment. In: Janick J, editor. Plant Breeding Reviews. Hoboken (NJ): Wiley?Blackwell; 2015. p. 23–88. https://doi.org/10.1002/9781119107743.ch02
  5. Ulukapi K, Nasircilar AG. Developments of gamma ray application on mutation breeding studies in recent years. Proceed of the Int Conf on Adv in Agri, Biol and Environ Sci. London, UK; 2019 Feb. 32-41.
  6. Ahloowalia B, Maluszynski M, Nichterlein K. Global impact of mutation-derived varieties. Euphytica. 2004;135(2):187–204. https://doi.org/10.1023/B:EUPH.0000014914.85465.4f
  7. Ali S, Suryakant TN. Mutation Breeding and Its Importance in Modern Plant Breeding: A Review. Exp Agric Int, 2024;46(7):264-275. https://doi.org/10.9734/jeai/2024/v46i72581
  8. Oladosu Y, Rafii M.Y, Abdullah N, Hussin G, Ramli A, Rahim H.A, Usman M. Principle and application of plant mutagenesis in crop improvement: a review. Biotechnol Biotechnol Equip, 216;30(1):1-16. https://doi.org/10.1080/13102818.2015.1087333
  9. Khan SJ, Khan HU, Khan RD, Iqbal MM, Zafar Y. Development of sugarcane mutants through in vitro mutagenesis. Pak J Biol Sci. 2000;3(7):1123-1125. https://doi.org/10.3923/pjbs.2000.1123.1125
  10. Mba C. Induced mutations unleash the potentials of plant genetic resources for food andagriculture. Agron. 2013;(3):200-231. https://doi.org/10.3390/agronomy3010200
  11. Hearn CJ. Development of seedless grapefruit cultivars through budwood irradiation. J Am Soc Hortic Sci. 1986;111(2):304-306. https://doi.org/10.21273/jashs.111.2.304.
  12. Maluszynski M, Ahloowalia BS, Sigurbjörnsson B. Application of in vivo and in vitro mutation techniques for crop improvement. Euphytica. 1995;85(3):303-315. https://doi.org/10.1007/BF00023960
  13. Salve KM, More AD. Effect of gamma radiation on seed germination, seedling height and seedling injury in Coriandrum sativum Linn. Int J Life Sci. 2014;2(3):223-5
  14. Sikder S, Biswas P, Hazra P, Akhtar S, Chattopadhyay A, Badigannavar AM, et al. Induction of mutation in tomato (Solanum lycopersicum L.) by gamma irradiation and EMS. Indian J Genet Plant Breed. 2013;73(4):392-399. https://doi.org/10.5958/j.0975-6906.73.4.059
  15. Puchooa D. In vitro mutation breeding of Anthurium by gamma radiation. Int J Agri Biol. 2005;7(1):11-20
  16. Wi SG, Chung BY, Kim J-H, Baek M-H, Yang DH, Lee J-W, et al. Ultrastructural changes of cell organelles in Arabidopsis stem after gamma irradiation. J Plant Biol. 2005;48:195-200. https://doi.org/10.1007/BF03030408
  17. Jain SM. Major mutation-assisted plant breeding programs supported by FAO/IAEA. Plant Cell Tiss Organ Cult. 2005;82(1):113–123. https://doi.org/10.1007/s11240-004-7095-6
  18. Jain SM. Mutagenesis in crop improvement under climate change. Rom Biotechnol. Lett. 2010;15(2):88-106.
  19. Spencer Lopes MM, Forster BP, Jankuloski L. Manual on mutation breeding [Internet]. [26Oct, 2024]; Available from: https://openknowledge.fao.org/server/api/core/bitstreams/d66c1346-80c7-4cd2-bd5b be7e9451c17f/content.
  20. Raina A, Laskar RA, Khursheed S, Khan S, Parveen K, Amin R, et al. Induced physical and chemical mutagenesis for improvement of yield-attributing traits and their correlation analysis in chickpea. Int Lett Nat Sci. 2017;(61):14-22. https://doi.org/10.56431/p-x5xgek
  21. Ulukapi K, Ozdemir B, Onus AN. Determination of proper gamma radiation dose in mutation breeding in eggplant (Solanum melongena L.). In: Advances in Agriculture and Environmental Science. Proceedings of the 4th International Conference on Energy Systems, Environment, Entrepreneurship and Innovation (ICESEEI '15); 2015 Feb 22-24; Dubai, United Arab Emirates. p. 149-153
  22. Koornneef M. Classical mutagenesis in higher plants. In: Gilmartin PM, Bowler C, editors. Molecular Plant Biology. Oxford: Oxford University Press; 2002. p. 1-12
  23. Chopra VL. Mutagenesis Investigating the process and processing the outcome for crop improvement. Curr Sci. 2005;89(2):353-359
  24. Predieri S. Mutation induction and tissue culture in improving fruits. Plant Cell Tissue Organ Cult. 2001;64:185-210. https://doi.org/10.1023/A:1010623203554
  25. Saptadi D, Arisah H, Agisimanto D. Optimization of gamma ray irradiation dose on strawberry plantlets. IOP Conference Series: Earth and Environmental Science. 2020;883:012071. Presented at: International Seminar on Agriculture, Biodiversity, Food Security and Health; December 10, 2020; Ambon, Indonesia. https://doi.org/10.1088/1755-1315/883/1/012018
  26. Rawat M, Singh VP, Verma SK, Rai R, Srivastava R. Determination of LD50 dose for ethyl methane sulphonate induced mutagenesis in Bhagwa Pomegranate. Emergent Life Sci Res. 2023;9(2):61-67. https://doi.org/10.31783/elsr.2023.926167
  27. Rodge RR, Rajan R, Kaur H, Jabroot K, Pandey K. Standardization of EMS doses for mutagenesis in strawberry (Fragaria x Ananassa Duch) cv. Winter Dawn. Electron J Plant Breed. 2024; 15(3): 752-757. https://doi.org/10.37992/2024.1503.072
  28. Choudhary AD, Dnyansagar VR. Effect of physical and chemical mutagens on morphological parameters in garlic. J Indian Bot Soc. 1980;59 (3):202-206
  29. Saha RR, Sultana W. Influence of seed ageing on growth and yield of soybean. Bangladesh J Bot. 2008;37(1):21–26
  30. Perez de Camacaro ME, Camacaro GJ, Hadley P, Battey NH, Carew JG. Pattern of growth and development of the strawberry cultivars Elsanta, Bolero and Everest. J Amer Soc Hort Sci. 2002;127(6):901-907. https://doi.org/10.21273/JASHS.127.6.901
  31. Asare AT, Mensah F, Acheampong S, Asare-Bediako E, Armah J. Effects of gamma irradiation on agromorphological characteristics of okra (Abelmoschus esculentus L. Moench). Adv Agric. 2017; 2017: 2385106. https://doi.org/10.1155/2017/2385106
  32. Dwimahyani I, Widiarsih S. The effects of gamma irradiation on the growth and propagation of in-vitro chrysanthemum shoot explants cv. Yellow Puma. At Indones. 2010;36(2):45-49. https://doi.org/10.17146/aij.2010.25
  33. Yadav A, Singh B, Singh SD. Impact of gamma irradiation on growth, yield, and physiological attributes of maize. Indian J Exp Biol. 2017; 57(02):116-122
  34. Ferreira-Castro FL, Aquino S, Greiner R, Ribeiro DHB, Reis TA, Corrêa B. Effects of gamma radiation on maize samples contaminated with Fusarium verticillioides. Applied Radiation and Isotopes. 2007;65(08):927-933. https://doi.org/10.1016/j.apradiso.2007.03.011
  35. Esmail AS. Effect of some growth regulators and irradiation on propagation and anatomical structure of Dracaena surculosa Lind. and Beaucarnea recurvata Lam. plants by using tissue culture technique. [PhD Thesis]. Faculty of Agriculture, Cairo University; 2014.
  36. Aref IM, Khan PR, Al Sahli AA, Husen A, Ansari MKA, Mahmooduzzafar, Iqbal M. Response of Datura innoxia Linn. to gamma rays and its impact on plant growth and productivity. Proc Natl Acad Sci India Sect B Biol Sci. 2016;86:623-629. https://doi.org/10.1007/s40011-014-0485-6

Downloads

Download data is not yet available.