Optimization of gamma irradiation doses (60Co) for mutagenesis in strawberry (Fragaria × annanasa Duch) cv. Winter Dawn

Rahul R  Rodge; Rajni Rajan; Kuldeep Pandey; Harjinder Kaur; Khan  Jabroot; Madhurima Chaudhuri; Jyoti Sharma; Sunny   Sharma; Nirmal  Patil; A M Wagh; D N Bhosale; S M Karadak

doi:10.14719/pst.6270

Authors

Rahul R Rodge Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara 144 401, Punjab, India https://orcid.org/0000-0002-5404-1583
Rajni Rajan Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara 144 401, Punjab, India https://orcid.org/0000-0001-9103-7922
Kuldeep Pandey Department of Fruit Science, College of Horticulture & Forestry, Acharya Narendra Dev University of Agriculture and Technology, Ayodhya 224 229, Uttar Pradesh, India https://orcid.org/0000-0001-7347-5232
Harjinder Kaur Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara 144 401, Punjab, India https://orcid.org/0009-0000-7233-7671
Khan Jabroot Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara 144 401, Punjab, India https://orcid.org/0009-0008-8262-4728
Madhurima Chaudhuri Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara 144 401, Punjab, India https://orcid.org/0000-0001-5018-9815
Jyoti Sharma Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara 144 401, Punjab, India https://orcid.org/0009-0003-9386-5643
Sunny Sharma Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara 144 401, Punjab, India https://orcid.org/0000-0002-5813-5210
Nirmal Patil Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara 144 401, Punjab, India https://orcid.org/0009-0009-1119-4756
A M Wagh Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara 144 401, Punjab, India https://orcid.org/0000-0001-9311-1591
D N Bhosale Department of Horticulture, College of Agriculture Bhanashivare, Ahilya Nagar 414609, Maharashtra, India https://orcid.org/0000-0002-7321-0127
S M Karadak Department of Horticulture, College of Agriculture Bhanashivare, Ahilya Nagar 414609, Maharashtra, India https://orcid.org/0000-0002-7265-3671

DOI:

https://doi.org/10.14719/pst.6270

Keywords:

60Co, Fragaria × annanasa, gamma irradiation, mutagenesis, winter dawn

Abstract

Strawberry, a vegetatively propagated crop, exhibits limited genetic variation, which constrains its adaptability and improvement potential. Gamma-ray radiation is a promising approach for enhancing genetic diversity and improving strawberries. Strawberry runners of the Winter Dawn cultivar were irradiated using 60Co, with doses ranging from 20 Gy to 40 Gy administered at a consistent rate of 1.52 Gy per minute to evaluate mutagenic effects. The survival effects in gamma irradiation-treated runners were recorded at 30 days after transplantation. An LD50 analysis was conducted to assess physiological impacts, and the lethal dose value was found to be 40 Gy, resulting in 50% mortality. Distinct differences in survival rates were observed between treated and untreated runners, with the highest survival rate (88%) at 20 Gy. The 30 Gy dose-treated runners exhibited the highest growth parameters, including crown diameter (14.32 mm), primary root length (29.50 cm), secondary root length (5.5 cm), and number of roots (39.6). Among fruit quality traits, maximum fruit length (39.84 mm), and fruit diameter (27.80 mm) were observed in runners treated with a 30 Gy gamma irradiation dose. Significant variations in survival, growth, and fruit quality were observed across different gamma irradiation doses. These findings provide a foundation for future efforts to develop potential strawberry mutants with improved traits through gamma irradiation. These findings provide a foundation for future efforts to develop potential strawberry mutants with improved traits through gamma irradiation.

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