Biocatalytic conversion of syngas to liquid biofuels using Clostridium acetobutylicum
DOI:
https://doi.org/10.14719/pst.6320Keywords:
acetone, biomass, butanol, ethanol, gasification, syngasAbstract
This study aimed to develop a pilot-scale bioreactor for ethanol production from syngas produced from biomass gasification and evaluate its performance. Gasification of characterized feedstock, namely Casuarina wood and coconut shell, is performed in a 2 kg hr-1 downdraft gasifier and the produced gas is cooled in a heat exchanger and scrubbed in a scrubbing bed to produce syngas free of moisture, tar and particulates. The syngas are fermented to produce biofuels in a 5 L capacity bioreactor with Clostridium acetobutylicum strains. The percentage yield of acetone, butanol and ethanol was verified with the standard concentration and found to be 12%, 5% and 7% from casuarina wood and 13%, 7% and 6% from coconut shell respectively. The syngas components viz., carbon monoxide (CO), carbon dioxide (CO2) and hydrogen (H), are fermented to produce acetone, butanol and ethanol along with larger proportions of acids. These acids can be converted into bio-alcohol if the fermentation period or gas-liquid transfer is enhanced. The pretreatment of feedstock is not required in syngas fermentation as it is a major process in conventional bio-alcohol production methods. This pays a way to superior technology in terms of cost and time.
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Xia A, Lin K, Zhu T, Huang Y, Zhu X, Zhu X, et al. Improving the saccharification efficiency of lignocellulosic biomass using a bio-inspired two-stage microreactor system loaded with complex enzymes. Green Chem. 2022;24:9519-29. https://doi.org/10.1039/D2GC02965K
Pavolova H, Bakalár T, Kyše?a K, Klimek M, Hajduova Z, Zawada M. The analysis of investment into industries based on portfolio managers. Acta Montan Slovaca. 2021;26(1):161-70. https://doi.org/10.46544/AMS.v26i1.14
Akbari M, Loganathan N, Tavakolian H, Mardani A, Štreimikien? D. The dynamic effect of micro-structural shocks on private investment behavior. Acta Montan Slovaca. 2021;26(1):1-17. https://doi.org/10.46544/AMS.v26i1.01
Maroušek J. Aluminum nanoparticles from liquid packaging board improve the competitiveness of (bio) diesel. Clean Technol Environ Policy. 2023;25(3):1059-67. https://doi.org/10.1007/s10098-022-02413-y
Maroušek J, Gavurová B, Strunecký O, Maroušková A, Sekar M, Marek V. Techno-economic identification of production factors threatening the competitiveness of algae biodiesel. Fuel. 2023;344:128056. https://doi.org/10.1016/j.fuel.2023.128056
Demirbas A. Progress and recent trends in biofuels. Prog Energy Combust Sci. 2007;33:1–18. https://doi.org/10.1016/j.pecs.2006.06.001
Perret L, Boukis N, Saue J. Synthesis gas fermentation at high cell density: How pH and hydrogen partial pressure affect productivity and product ratio in continuous fermentation. Bioresour Technol. 2024;391A:29894. https://doi.org/10.1016/j.biortech.2023.129894
Liew FM, Martin ME, Tappel RC, Heijstra BD, Mihalcea C, Kopke M. Gas fermentation – A flexible platform for commercial scale production of low carbon fuels and chemicals from waste and renewable feedstocks. Front Microbiol. 2016;11(7):694. https://doi.org/10.3389/fmicb.2016.00694
Sriramajayam S, Padhi T, Ramesh D, Gitanjali J, Palaniselvam V. Process optimization of simultaneous saccharification and fermentation system for bioethanol production from pearl millet stalk. Int J Agric Sci. 2022;14(6):11381?85.
Oswald F, Dörsam S, Veith N, Zwick M, Neumann A, Ochsenreither K, et al. Sequential mixed cultures: From syngas to malic acid. Front Microbiol. 2016;7:891. https://doi.org/10.3389/fmicb.2016.00891
Liakakou ET, Vreugdenhil BJ, Cerone N, Zimbardi F, Pinto F, André R, et al. Gasification of lignin-rich residues for the production of biofuels via syngas fermentation: Comparison of gasification technologies. Fuel. 2019;251:580–92. https://doi.org/10.1016/j.fuel.2019.04.081
Jack J, Lo J, Maness PC, Ren ZJ. Directing Clostridium ljungdahlii fermentation products via hydrogen to carbon monoxide ratio in syngas. Biomass Bioenergy. 2019;124:95–101. https://doi.org/10.1016/j.biombioe.2019.03.011
Maroušek J, Trakal L. Techno-economic analysis reveals the untapped potential of wood biochar. Chemosphere. 2022;291(Part-1):133000. https://doi.org/10.1016/j.chemosphere.2021.133000
Pardo-Planas O, Atiyeh HK, Phillips JR, Aichele CP, Mohammad S. Process simulation of ethanol production from biomass gasification and syngas fermentation. Bioresour Technol. 2017;245(Part A):925–32. https://doi.org/10.1016/j.biortech.2017.08.193
Fernández NA, Veiga MC, Kennes C. Selective anaerobic fermentation of syngas into either C2-C6 organic acids or ethanol and higher alcohols. Bioresour Technol. 2019;280:387-95. https://doi.org/10.1016/j.biortech.2019.02.018
Sun X, Atiyeh HK, Huhnke RL, Tanner RS. Syngas fermentation process development for production of biofuels and chemicals: A review. Bioresour Technol Rep. 2019;7:100279. https://doi.org/10.1016/j.biteb.2019.100279
Rückel A, Hannemann J, Maierhofer C, Fuchs A, Weuster-Botz D. Studies on syngas fermentation with Clostridium carboxidivorans in stirred-tank reactors with defined gas impurities. Front Microbiol. 2021;12:655390. https://doi.org/10.3389/fmicb.2021.655390
Wainaina S, Horváth IS, Taherzadeh MJ. Biochemicals from food waste and recalcitrant biomass via syngas fermentation: A review. Bioresour Technol. 2018;248(Part-A):113-121. https://doi.org/10.1016/j.biortech.2017.06.075
Ragsdale SW. Enzymology of the acetyl-CoA pathway of CO2 fixation. Crit Rev Biochem Mol Biol. 1991;26(3-4):261–300. https://doi.org/10.3109/10409239109114070
Henstra AM, Sipma J, Rinzema A, Stams AJM. Microbiology of synthesis gas fermentation for biofuel production. Curr Opin Biotechnol. 2007;18(3):200–206. https://doi.org/10.1016/j.copbio.2007.03.008
Shen N, Xia XY, Zeng RJ, Zhang F. Conversion of syngas (CO and H2) to biochemicals by mixed culture fermentation in mesophilic and thermophilic hollow-fiber membrane biofilm reactors. J Clean Prod. 2018;202:536–542. https://doi.org/10.1016/j.jclepro.2018.08.162
Dogru M, Howrath CR, Akay G, Keskinler B, Malik AA. Gasification of hazelnut shells in a downdraft gasifier. Energy. 2002;27(5):415-27. https://doi.org/10.1016/S0360-5442(01)00094-9
Munasinghe PC, Khanal SK. Biomass-derived syngas fermentation into biofuels: Opportunities and challenges. Bioresour Technol. 2010;101(13):5013-22. https://doi.org/10.1016/j.biortech.2009.12.098
Xu D, Tree DR, Lewis RS. The effects of syngas impurities on syngas fermentation to liquid fuels. Biomass Bioenergy. 2011;35(7):2690–96. https://doi.org/10.1016/j.biombioe.2011.03.005
Bain RL, Broer K. Gasification. In: Brown RC, editor. Thermochemical processing of Biomass: Conversion into Fuels, Chemicals and Power. Hoboken (NJ): Wiley Online library; 2011:47–77 https://doi.org/10.1002/9781119990840.ch3
Nastaj J, Ambro?ek B. Analysis of gas dehydration in TSA system with multi-layered bed of solid adsorbents. Chem Eng Process: Process Intensif. 2015;96:44–53. https://doi.org/10.1016/j.cep.2015.08.001
Saleem J, Shahid UB, Hijab M, Mackey H, Mckay G. Production and applications of activated carbons as adsorbents from olive stones. Biomass Conv Bioref. 2019;9:775–802. https://doi.org/10.1007/s13399-019-00473-7
McKendry P. Energy production from biomass (part 3): gasification technologies. Bioresour Technol. 2002;83(1):55-63. https://doi.org/10.1016/S0960-8524(01)00120-1
Monir MU, Abd Aziz A, Khatun F, Yousuf A. Bioethanol production through syngas fermentation in a tar free bioreactor using Clostridium butyricum. Renew Energy. 2020;157:1116-23.https://doi.org/10.1016/j.renene.2020.05.099
Xu D, Lewis RS. Syngas fermentation to biofuels: Effects of ammonia impurity in raw syngas on hydrogenase activity. Biomass Bioenergy. 2012;45:303–310. https://doi.org/10.1016/j.biombioe.2012.06.022
Abubackar HN, Veiga MC, Kennes C. Production of acids and alcohols from syngas in a two-stage continuous fermentation process. Bioresour Technol. 2018;253:227–234. https://doi.org/10.1016/j.biortech.2018.01.026
Jiang M, Chen JN, He AY, Wu H, Kong XP, Liu JL, et al. Enhanced acetone/butanol/ethanol production by Clostridium beijerinckii IB4 using pH control strategy. Process Biochem. 2014;49(8):1238-44. https://doi.org/10.1016/j.procbio.2014.04.017
Sathish A, Sharma A, Gable P, Skiadas I, Brown R, Wen, Z. A novel bulk-gas-to-atomized-liquid reactor for enhanced mass transfer efficiency and its application to syngas fermentation. Chem Eng J. 2019;370:60-70. https://doi.org/10.1016/j.cej.2019.03.183
Minofar B, Mil?i? N, Maroušek J, Gavurová B, Maroušková A. Understanding the molecular mechanisms of interactions between biochar and denitrifiers in N?O emissions reduction: Pathway to more economical and sustainable fertilizers. Soil Tillage Res. 2025;248:106405. https://doi.org/10.1016/j.still.2024.106405
Valaskova K, Nagy M, Grecu G. Digital twin simulation modeling, artificial intelligence-based internet of manufacturing things systems and virtual machine and cognitive computing algorithms in the industry 4.0-based Slovak labor market. Oecon Copernic. 2024;15(1):95-143. https://doi.org/10.24136/oc.2814
Skare M, Porada-Rochon M, Blazevic-Buric S. Energy cycles: Nature, turning points and role in England economic growth from 1700 to 2018. Acta Montan Slovaca. 2021;26(2): 281-302. https://doi.org/10.46544/AMS.v26i2.08
Zheng Z, Xu Z, Skare M, Porada-Rochon M. A comprehensive bibliometric analysis of the energy poverty literature: from 1942 to 2020. Acta Montan Slovaca. 2021;26(3):512-33. https://doi.org/10.46544/AMS.v26i3.10
Klieštik T, Kral P, Bugaj M, Durana P. Generative artificial intelligence of things systems, multisensory immersive extended reality technologies and algorithmic big data simulation and modelling tools in digital twin industrial metaverse. Equilib Q J Econ Econ Policy. 2024;19(2):429-61. https://doi.org/10.24136/eq.3108
Kliestik T, Nica E, Durana P, Popescu GH. Artificial intelligence-based predictive maintenance, time-sensitive networking and big data-driven algorithmic decision-making in the economics of industrial internet of things. Oecon Copernic. 2023;14(4):1097-138. https://doi.org/10.24136/oc.2023.033
Dvorský J, Bednarz J, Blajer-Go??biewska A. The impact of corporate reputation and social media engagement on the sustainability of SMEs: Perceptions of top managers and the owners. Equilib Q J Econ Econ Policy. 2023;18(3):779-811. https://doi.org/10.24136/eq.2023.025
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Copyright (c) 2025 Ponnaian Vijayakumary, Gitanjali Jothiprakash, Srinivasan Sriramajayam , Subburamu Karthikeyan, P Subramanian , Desikan Ramesh
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