Skip to main navigation menu Skip to main content Skip to site footer
Plant Science Today (PST)

Research Articles

Early Access

Unveiling the rhizosphere microbial community of castor plant (Ricinus communis L.) grown in textile effluent contaminated ecosystem and assessing its phylogenetic traits for plant growth promotion

DOI
https://doi.org/10.14719/pst.6504
Submitted
3 December 2024
Published
03-04-2025
Versions

Abstract

Various factors influence the microbial community in the plant rhizosphere, including the variety, activity, and population structure. Plant species and soil type are critical determinants of the composition and diversity of the microbial communities linked to plant roots. Understanding these interactions is crucial for enhancing plant development and soil vitality, especially under adverse conditions such as salt-affected soils. This study evaluated the structure and composition of rhizosphere microbial communities in response to castor plant (Ricinus communis L.), a crop known for its resilience to various soil conditions. The bacterial community in the castor rhizosphere was examined by 16S rDNA sequencing in conjunction with an assessment of various plant growth-promoting (PGP) characteristics of the isolated bacterial strains. These findings indicated that the rhizosphere bacterial community was primarily comprised of Bacillus species, which are essential plant growth-promoting rhizobacteria (PGPR). These bacteria exhibit considerable potential to augment nutrient absorption, strengthen stress resilience, and promot overall plant vitality. Their supremacy underscores their adaptability and functional significance in castor root systems, particularly under diverse soil conditions. This study elucidates the microbial dynamics of the castor rhizosphere, highlighting the crucial role of Bacillus species in enhancing plant growth and soil fertility. These findings indicate that Bacillus-dominated microbial communities can be efficiently utilized for sustainable agriculture and soil remediation initiatives.

References

  1. Dobbelaere S, Vanderleyden J, Okon Y. Plant growth-promoting effects of diazotrophs in the rhizosphere. Crit Rev Plant Sci. 2003;22(2):107–49. https://doi.org/10.1080/713610853
  2. Gray EJ, Smith DL. Intracellular and extracellular PGPR: Commonalities and distinctions in the plant-bacterium signaling processes. Soil Biol Biochem. 2005;37(3):395–412. https://doi.org/10.1016/j.soilbio.2004.08.030
  3. Weller DM, Thomashow LS. Current challenges in introducing beneficial microorganisms into the rhizosphere. In: O’Gara F, Dowling DN, Boesten B, editors. Molecular Ecology of Rhizosphere Microorganisms’ Biotechnology and the Release of GMO. Wiley-VCH Verlag, Weinheim, Baden-Württemberg ; 1994. p. 1–18 https://doi.org/10.1002/9783527615810.ch1
  4. Schroth MN, Hancock JG. Disease suppressive soil and root-colonizing bacteria. Science. 1982;216:1376–81. https://doi.org/10.1126/science.216.4553.1376 .
  5. Jang JH, Woo SY, Kim SH, Khaine I, Kwak MJ, Lee HK, et al. E?ects of increased soil fertility and plant growth-promoting rhizobacteria inoculation on biomass yield, energy value and physiological response of poplar in short-rotation coppices in a reclaimed tideland: A case study in the Saemangeum area of Korea. Biomass Bioenergy. 2017;107:29–38. https://doi.org/10.1016/j.biombioe.2017.09.005
  6. Hayat R, Ali S, Amara U, Khalid R, Ahmed I. Soil beneficial bacteria and their role in plant growth promotion: A review. Ann Microbiol. 2010;60:579–98. https://doi.org/10.1007/s13213-010-0117-1
  7. Fiallo-Olivé E, Palacio-Bielsa A, Sacristán S. Plant pathogenic microorganisms: State-of-the-Art Research in Spain. Microorganisms. 2023;11(3):816. https://doi.org/10.3390/microorganisms11030816
  8. Martins PMM, Merfa MV, Takita MA, De Souza AA. Persistence in phytopathogenic bacteria: Do we know enough?. Front Microbiol. 2018;9:1099. https://doi.org/10.3389/fmicb.2018.01099
  9. Das T, Bhattacharyya A, Bhar A. Linking phyllosphere and rhizosphere microbiome to the plant-insect interplay: The new dimension of tripartite Interaction. Physiologia. 2023;3(1):129–44. https://doi.org/10.3390/physiologia3010011
  10. Wani PA, Khan MS, Zaidi A. Chromium reduction, plant growth–promoting potentials and metal solubilizatrion by Bacillus sp. isolated from alluvial soil. Curr Microbiol. 2007;54:237–43. https://doi.org/10.1007/s00284-006-0451-5
  11. Singh P, Chauhan PK, Upadhyay SK, Singh RK, Dwivedi P, Wang J, et al. Mechanistic insights and potential use of siderophores producing microbes in rhizosphere for mitigation of stress in plants grown in degraded land. Front Microbiol. 2022;13:898979. https://doi.org/10.3389/fmicb.2022.898979
  12. Han L, Zhang H, Xu Y, Li Y, Zhou J. Biological characteristics and salt-tolerant plant growth-promoting effects of an ACC deaminase producing Burkholderia pyrrocinia strain isolated from the tea rhizosphere. Arch Microbiol. 2021;203:2279–90. https://doi.org/10.1007/s00203-021-02204-x
  13. Khalifa A, Aldayel M. Isolation and characterization of Klebsiella oxytoca from the rhizosphere of Lotus corniculatus and its biostimulating features. Braz J Biol. 2022;82:e266395. https://doi.org/10.1590/1519-6984.266395
  14. Parvin N, Mukherjee B, Roy S, Dutta S. Characterization of plant growth promoting rhizobacterial strain Bacillus cereus with special reference to exopolysaccharide production. J Plant Nutr. 2023;46(11):2608–20. https://doi.org/10.1080/01904167.2022.2160740
  15. Fagorzi C, Checcucci A, DiCenzo GC, Debiec-Andrzejewska K, Dziewit L, Pini F, et al. Harnessing rhizobia to improve heavy-metal phytoremediation by legumes. Genes. 2018;9(11):542. https://doi.org/10.3390/genes9110542
  16. Feng Q, Cao S, Liao S, Wassie M, Sun X, Chen L, et al. Fusarium equiseti-inoculation altered rhizosphere soil microbial community, potentially driving perennial ryegrass growth and salt tolerance. Sci Total Environ. 2023;871:162153 https://doi.org/10.1016/j.scitotenv.2023.162153
  17. Priya P, Aneesh B, Sivakumar KC, Harikrishnan K. Comparative proteomic analysis of saline tolerant, phosphate solubilizing endophytic Pantoea sp. and Pseudomonas sp. isolated from Eichhornia rhizosphere. Microbiol Res. 2022;265:127217 https://doi.org/10.1016/j.micres.2022.127217
  18. Nofal MM, Attia MS. Management of root-knot nematode (Meloidogyne incognita) and improve defensive ability of cucumber plant using Serratia marcescens, salicylic acid and fosthiazate (melotherin®) through adaptive processes and antioxidant capacity. Menoufia J Plant Prot. 2023;8(3):31–45. https://doi.org/10.21608/mjapam.2023.197242.1011
  19. Sriwati R, Maulidia V, Intan N, Oktarina H, Khairan K, Skala L, et al. Endophytic bacteria as biological agents to control fusarium wilt disease and promote tomato plant growth. Physiol Mol Plant Pathol. 2023;125:101994. https://doi.org/10.1016/j.pmpp.2023.101994
  20. Kloepper JW, Lifshitz R, Zablotowicz RM. Free-living bacterial inocula for enhancing crop productivity. Trends Biotechnol. 1989;7(2):39–44. https://doi.org/10.1016/0167-7799(89)90057-7
  21. Marroni IV. Screening of bacteria of the genus Bacillus for the control of the plant-pathogenic fungus Macrophomina phaseolina. Biocontrol Sci Tech. 2015;25(3):302–15. https://doi.org/10.1080/09583157.2014.976178
  22. Rajkumar M, Freitas H. Influence of metal resistant-plant growth-promoting bacteria on the growth of Ricinus Communis in soil contaminated with heavy metals. Chemosphere. 2008;71(5):834–42. https://doi.org/10.1016/j.chemosphere.2007.11.038
  23. Ma Y, Prasad MNV, Rajkumar M, Freitas H. Plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soils. Biotechnol Adv. 2011;29(2):248–58. https://doi.org/10.1016/j.biotechadv.2010.12.001
  24. Kumar P, Dubey RC, Maheshwari DK. Bacillus strains isolated from the rhizosphere showed plant growth promoting and antagonistic activity against phytopathogens. Microbiol Res. 2012;167(8):493–99.
  25. https://doi.org/10.1016/j.micres.2012.05.002
  26. Maheshwari MN, Solanki VA. Isolation of native isolates of fluorescent pseudomonads from castor wilt field in North Gujarat area. Int J Plant Prot. 2012;5(2):444–45. https://api.semanticscholar.org/CorpusID:82183328
  27. Sandilya SP, Bhuyan PM, Gogoi DK, Kardong D. Phosphorus solubilization and plant growth promotion ability of rhizobacteria of R. communis L growing in Assam, India. Proc Natl Acad Sci India Sect B Biol Sci. 2018;88:959–66. https://doi.org/10.1007/s40011-016-0833-9
  28. Mohanram S, Kumar P. Rhizosphere microbiome: revisiting the synergy of plant-microbe interactions. Ann Microbiol. 2019;69:307–20. https://doi.org/10.1007/s13213-019-01448-9
  29. Gilcreas FW. Standard methods for the examination of water and waste water. Am J Public Health Nations Health. 1966;56(3):387–88. https://doi.org/10.2105/ajph.56.3.387
  30. Patel MM, Joshi BM, Parmar RB, Singh NK, Tapre PV. Characterization of plant growth promoting endophytic bacteria from castor plants [Ricinus communis (L.)]. Int J Bioresour Stress Mgmt. 2022;13(12):1381–90. https://doi.org/10.23910/1.2022.3240
  31. Srivastav S, Yadav KS, Kundu BS. Prospects of using phosphate solubilizing Pseudomonas as biofungicide. Indian J Microbiol. 2004;44:91–94. https://doi.org/10.5829/idosi.ejbs.2012.4.2.63117
  32. Alexander DB, Zuberer DA. Use of chrome Azul S reagents to evaluate siderophore production by rhizosphere bacteria. Biol Ferti Soils. 1991;12:39–45. https://doi.org/10.1007/BF00369386
  33. Weidner S, Arnold W, Puhler A. Diversity of uncultured microorganisms associated with the seagrass Halophila stipulacea estimated by restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes. Appl Environ Microbiol. 1996;62(3):766–71. https://doi.org/10.1128/aem.62.3.766-771.1996
  34. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol. 2013;30(12):2725–29. https://doi.org/10.1093/molbev/mst197
  35. Vos PD, Garrity GM, Jones D, Krieg NR, Ludwig W, Rainey FA, Schleifer KH, Whitman WB. Bergey's manual of systematic bacteriology. The Firmicutes, Springer Dordrecht Heidelberg, London, New York. 2009;2(3):1450. ISBN: 978-0-387-95041-9, eBook ISBN978-0-387-68489-5. https://doi.org/10.1007/b92997
  36. Tsotetsi T, Nephali L, Malebe M, Tugizimana F. Bacillus for plant growth promotion and stress resilience: What have we learned?. Plants. 2022;11(19):2482. https://doi.org/10.3390/plants11192482
  37. Sun Z, Liu K, Zhang J, Zhang Y, Xu K, Yu D, et al. IAA producing Bacillus altitudinis alleviates iron stress in Triticum aestivum L. seedling by both bioleaching of iron and up-regulation of genes encoding ferritins. Plant Soil. 2017;419:1–11. https://doi.org/10.1007/s11104-017-3218-9
  38. Sultana S, Alam S, Karim MM. Screening of siderophore-producing salt-tolerant rhizobacteria suitable for supporting plant growth in saline soils with iron limitation. J Agric Food Res. 2021;4:100150. https://doi.org/10.1016/j.jafr.2021.100150
  39. Pattnaik S, Mohapatra B, Gupta A. Plant growth-promoting microbe mediated uptake of essential nutrients (Fe, P, K) for crop stress management: Microbe-soil-plant continuum. Front Agron. 2021;3:689972. https://doi.org/10.3389/fagro.2021.689972
  40. Yadav RC, Sharma SK, Varma A, Singh UB, Kumar A, Bhupenchandra I, et al. Zinc-solubilizing Bacillus spp. in conjunction with chemical fertilizers enhance growth, yield, nutrient content and zinc biofortification in wheat crop. Front Microbiol. 2023;14:1210938. https://doi.org/10.3389/fmicb.2023.1210938
  41. Chen Y, Ye J, Kong Q. Potassium-solubilizing activity of Bacillus aryabhattai SK1-7 and its growth-promoting effect on Populus alba L. Forests. 2020;11(12):1348. https://doi.org/10.3390/f11121348
  42. Cendales TC, Cristian G, Claudia C, Omar T, Annia HR. Bacillus effect on the germination and growth of tomato seedlings (Solanum lycopersicum L). Acta Biol Colomb. 2017;22:37. https://doi.org/10.15446/abc.v22n1.57375

Downloads

Download data is not yet available.