In Vitro Antiacne and Antidandruff activity of extracted stigmasterol from seed waste of safflower (Carthamus tinctorius L.)
DOI:
https://doi.org/10.14719/pst.2019.6.sp1.670Keywords:
Anti-acne, Anti-dandruff activity, stigmasterolAbstract
The present study deals with extraction of saponins and their characterization from the seed wastes of safflower. The presence of stigmasterol in the extracted safflower seed waste (S.W.S) was confirmed by Thin Layer Chromatography (TLC), followed by Fourier-Transform Infrared Spectroscopy (FTIR) and High Performance Liquid Chromatography (HPLC) on the basis of its peak compared with stigmasterol standard. FTIR showed the identical functional groups of butanolic extract of SSW with standard while TLC and HPLC showed their notable peak and retention time with the same. Further in-vitro antiacne and antidandruff microbial activity of extracted stigmasterol was confirmed by disc diffusion method. This preliminary study has exhibited anti-acne and anti dandruff potential of Safflower seed waste extract, in future which could be used in therapies and cosmetic applications.
Downloads
Download data is not yet available.
References
1. Andrews JM. Determination of minimum inhibitory concentrations. Journal of Antimicrobial Chemotherapy. 2001;48:5-16. https://doi.org/10.1093/jac/48.suppl_1.5
2. Saxena HO, Soni A, Mohammad N, Choubey SK. Phytochemical screening and elemental analysis in different plant parts of Uraria picta Desv, A Dashmul species. Journal of Chemical and Pharmaceutical Research. 2014;6:756-60.https://www.researchgate.net/publication/265849774
3. Negi JS, Negi PS, Pant GJ, Rawat MSM, Negi SK. Naturally occurring saponins: Chemistry and biology. Journal of Poisonous and Medicinal Plant Research. 2013;1:01-06. http://apexjournal.org/jpmpr/archive/2013/May/fulltext/Negi%20et%20al.pdf
4. Murugan T, Albino WJ, Murugan M. Antimicrobial Activity and Phytochemical Constituents of Leaf Extracts of Cassia auriculata. Indian Journal of Pharmacological Sciences. 2013;75:122–25. https://doi.org/10.1155/2013/621459
5. Mandal P, Sinha BSP, Mandal NC. Antimicrobial activity of saponins from Acacia auriculiformis. Fitoterapia. 2005;76:462-65. https://doi.org/10.1016/j.fitote.2005.03.004
6. Nagesh KS, Shanthamma C. Antibacterial activity of Curculigoorchioides rhizome extract on pathogenic bacteria. African Journal of Microbiology Research. 2009;3:05–09. http://www.academicjournals.org/app/webroot/article/article1380127779_Nagesh%20and%20%20Shanthamma.pdf
7. Mandal S, Guptan P, Owusu-Ansah E, Banerjee U. Mitochondrial regulation of cell cycle progression during development as revealed by the tenured mutation revealed in Drosophila. Developmental Cell.2005;9:843–54. https://doi.org/10.1016/j.devcel.2005.11.006
8. Rowland JRJ. Dryland Farming in Africa. London and Basingstoke: Macmillan; Wageningen. Published in cooperation with Technical Centre for Agricultural and Rural Cooperation. 1993;336. https://cgspace.cgiar.org/handle/10568/49220
9. Heuze V, Tran G, Chapoutot P, Renaudeau D, Bastianelli D, Lebas F. Safflower (Carthamus tinctorius) seeds and oil meal. Feedipedia, a programme by INRA, CIRAD, AFZ & FAO, 2015;10:51. https://agritrop.cirad.fr/582514/1/ID582514.pdf
10. Trease GE, Evans WS. Pharmacology, 11th edn. Bailliere Tindall Ltd, London, 1978; pp. 60–75. https://doi.org/10.4236/ns.2014.61003
11. Wei L, Sun Y, Yan T, Yang YS, Lee JS, Byun JH, Moon CH, Han SC, Young HK. Isolation of Nematicidal Triterpenoid Saponins from Pulsatilla koreana root and their activities against. Meloidogyne incognita. Molecules. 2011;16:4438–50. https://doi.org/10.3390/molecules18055306
12. Hassan SM, Aqil AAAL, Attimarad M. Determination of crude saponin and total flavonoids content in guar meal. Advancement in Medicinal Plant Research. 2013;1:24–28. http://www.netjournals.org/z-AMPR-13-013.html
13. Narayanan B, Sakthivel N. Coriander leaf mediated biosynthesis of gold nanoparticles. Material Letters. 2008;62: 4588–90. https://www.researchgate.net/publication/222011022
14. Sasidharan S, Chen Y, Saravanan D, Sundram KM, Yoga LL. Extraction, isolation and characterization of bioactive compounds from plants' extracts. African Journal of Traditional, Complementary & Alternative Medicines. 2011; 8:1–10. https://ncbi.nlm.nih.gov/pmc/articles/PMC3218439
15. Negi JS, Singh P., Pant GJN, Rawat MSM. High performance liquid chromatography analysis of plant saponins. Pharmacognosy. 2011;5:155-58. https://doi.org/10.4103/0973-7847.91109
16. Collins CH, Lyne PH. Microbiological methods, 8th edn. Arnold publisher, London. 1970; pp. 168–86. https://mmstcchemistry.weebly.com/uploads/2/4/1/2/24121933/microbiological_methods.pdf
17. Sibi G, Gurumeetkaur GD, Dhananjaya K, Ravikumar KR, Mallesha. Anti-dandruff activity of Ricinus communis L. leaf extracts. International Journal of Current Pharmaceutical Research. 2012;4:74-76.
18. Santhi K, Sengottuvel R. Qualitative and Quantitative Phytochemical analysis of Moringa concanensis Nimmo. International Journal of Current Microbiology and Applied Science. 2016;5:633-40. https://doi.org/10.20546/ijcmas.2016.501.064
19. Zhou FR, Zhao MB, Tu PF. Simultaneous determination of four nucleosides in Carthamus tinctorius L. and Safflower injection using high performance liquid chromatography. Journal of Chinese Pharmaceutical Sciences. 2009;18:326-30.
20. Oleszek W, Bialy Z. Chromatographic determination of plant saponins-an update 2002-2005. Journal of Chromatography A. 2006;1112:78–91. https://doi.org/10.1016/j.chroma.2006.01.037
21. Rengasamy R., Raja Kannan R., Arumugam R., Anantharaman P. Antibacterial potential of three seagrasses against human pathogens. Asian Pacific Journal of Tropical Medicine. 2010;3:890-93. https://www.researchgate.net/publication/286915540
22. Oleszek WA. Chromatographic determination of plant saponins. Journal of Chromatography A. 2002;967:147–62. https://doi.org/10.1016/s0021-9673(01)01556-4
23. In-Seon P, Eun MK, Namsoo K. High-Performance Liquid Chromatographic Analysis of Saponin Compounds in Bupleurum falcatum. Journal of Chromatographic Science. 2000;38. https://doi.org/10.1093/chromsci/38.6.229
24. Ankur G, Surabhi M, Rajendra S. Evaluation of antimicrobial activity of Curcuma longa rhizome extract against Staphylococcus aureus. Biotechnology Reports. 2015;6:51-55.https://doi.org/10.1016/j.btre.2015.02.001
25. Meena DS, Pavithra MKS. Antifungal activity by ethanolic extracts of medicinal plants against Malassezia furfur: A potential application in the treatment of Dandruff. International Journal of Pharmtech Research. 2015;8:440-43. http://sphinxsai.com/2015/ph_vol8_no3/3/(440-443)V8N3.pdf
26. Liu H, Crawford JH, Pierce RB, Norris P, Platnick SE, Chen G, et al. Radiative effect of clouds on tropospheric chemistry in a global three-dimensional chemical transport model. Journal of Geophysical Research. 2006;111:D20303. https://doi.org/10.1029/2005JD006403
27. Innocent CO, Chinelo AE. Phytochemical screening and in vitro antimicrobial activity of various parts of Cleome ciliata Schum. & Thonn. International Journal of Research Sstudies in Biosciences. 2019;11:2018. https://academic-oup-com.eres.qnl.qa/biohorizons/article/doi/10.1093/biohorizons/hzy018/5304541
28. Sagar DR, Kelly S, Millns PJ, O’Shaughnessey CT, Kendall DA, Chapman V. Inhibitory effects of CB1 and CB2 receptor agonists on responses of DRG neurons and dorsal horn neurons in neuropathic rats. European Journal of Neurosciences. 2005;22:371–79. https://doi.org/10.1111/j.1460-9568.2005.04206.x
29. Khan MI, Ahhmed A, Shin JH, Baek JS, Kim MY, Kim JD. Green tea seed isolated saponins exerts antibacterial effects against various strains of gram positive and gram negative bacteria, a comprehensive study in vitro and in vivo. Evidence Based Complementary and Alternative Medicine. 2018;2018:12. https://doi.org/10.1155/2018/3486106
30. Murugan T, Albino JW, Murugan M. Antimicrobial activity and phytochemical constituents of leaf extracts of Cassia auriculata. Indian Journal of Pharmaceutical Sciences. 2013;75:122–25. https://doi.org/10.4103/0250-474X.113546
2. Saxena HO, Soni A, Mohammad N, Choubey SK. Phytochemical screening and elemental analysis in different plant parts of Uraria picta Desv, A Dashmul species. Journal of Chemical and Pharmaceutical Research. 2014;6:756-60.https://www.researchgate.net/publication/265849774
3. Negi JS, Negi PS, Pant GJ, Rawat MSM, Negi SK. Naturally occurring saponins: Chemistry and biology. Journal of Poisonous and Medicinal Plant Research. 2013;1:01-06. http://apexjournal.org/jpmpr/archive/2013/May/fulltext/Negi%20et%20al.pdf
4. Murugan T, Albino WJ, Murugan M. Antimicrobial Activity and Phytochemical Constituents of Leaf Extracts of Cassia auriculata. Indian Journal of Pharmacological Sciences. 2013;75:122–25. https://doi.org/10.1155/2013/621459
5. Mandal P, Sinha BSP, Mandal NC. Antimicrobial activity of saponins from Acacia auriculiformis. Fitoterapia. 2005;76:462-65. https://doi.org/10.1016/j.fitote.2005.03.004
6. Nagesh KS, Shanthamma C. Antibacterial activity of Curculigoorchioides rhizome extract on pathogenic bacteria. African Journal of Microbiology Research. 2009;3:05–09. http://www.academicjournals.org/app/webroot/article/article1380127779_Nagesh%20and%20%20Shanthamma.pdf
7. Mandal S, Guptan P, Owusu-Ansah E, Banerjee U. Mitochondrial regulation of cell cycle progression during development as revealed by the tenured mutation revealed in Drosophila. Developmental Cell.2005;9:843–54. https://doi.org/10.1016/j.devcel.2005.11.006
8. Rowland JRJ. Dryland Farming in Africa. London and Basingstoke: Macmillan; Wageningen. Published in cooperation with Technical Centre for Agricultural and Rural Cooperation. 1993;336. https://cgspace.cgiar.org/handle/10568/49220
9. Heuze V, Tran G, Chapoutot P, Renaudeau D, Bastianelli D, Lebas F. Safflower (Carthamus tinctorius) seeds and oil meal. Feedipedia, a programme by INRA, CIRAD, AFZ & FAO, 2015;10:51. https://agritrop.cirad.fr/582514/1/ID582514.pdf
10. Trease GE, Evans WS. Pharmacology, 11th edn. Bailliere Tindall Ltd, London, 1978; pp. 60–75. https://doi.org/10.4236/ns.2014.61003
11. Wei L, Sun Y, Yan T, Yang YS, Lee JS, Byun JH, Moon CH, Han SC, Young HK. Isolation of Nematicidal Triterpenoid Saponins from Pulsatilla koreana root and their activities against. Meloidogyne incognita. Molecules. 2011;16:4438–50. https://doi.org/10.3390/molecules18055306
12. Hassan SM, Aqil AAAL, Attimarad M. Determination of crude saponin and total flavonoids content in guar meal. Advancement in Medicinal Plant Research. 2013;1:24–28. http://www.netjournals.org/z-AMPR-13-013.html
13. Narayanan B, Sakthivel N. Coriander leaf mediated biosynthesis of gold nanoparticles. Material Letters. 2008;62: 4588–90. https://www.researchgate.net/publication/222011022
14. Sasidharan S, Chen Y, Saravanan D, Sundram KM, Yoga LL. Extraction, isolation and characterization of bioactive compounds from plants' extracts. African Journal of Traditional, Complementary & Alternative Medicines. 2011; 8:1–10. https://ncbi.nlm.nih.gov/pmc/articles/PMC3218439
15. Negi JS, Singh P., Pant GJN, Rawat MSM. High performance liquid chromatography analysis of plant saponins. Pharmacognosy. 2011;5:155-58. https://doi.org/10.4103/0973-7847.91109
16. Collins CH, Lyne PH. Microbiological methods, 8th edn. Arnold publisher, London. 1970; pp. 168–86. https://mmstcchemistry.weebly.com/uploads/2/4/1/2/24121933/microbiological_methods.pdf
17. Sibi G, Gurumeetkaur GD, Dhananjaya K, Ravikumar KR, Mallesha. Anti-dandruff activity of Ricinus communis L. leaf extracts. International Journal of Current Pharmaceutical Research. 2012;4:74-76.
18. Santhi K, Sengottuvel R. Qualitative and Quantitative Phytochemical analysis of Moringa concanensis Nimmo. International Journal of Current Microbiology and Applied Science. 2016;5:633-40. https://doi.org/10.20546/ijcmas.2016.501.064
19. Zhou FR, Zhao MB, Tu PF. Simultaneous determination of four nucleosides in Carthamus tinctorius L. and Safflower injection using high performance liquid chromatography. Journal of Chinese Pharmaceutical Sciences. 2009;18:326-30.
20. Oleszek W, Bialy Z. Chromatographic determination of plant saponins-an update 2002-2005. Journal of Chromatography A. 2006;1112:78–91. https://doi.org/10.1016/j.chroma.2006.01.037
21. Rengasamy R., Raja Kannan R., Arumugam R., Anantharaman P. Antibacterial potential of three seagrasses against human pathogens. Asian Pacific Journal of Tropical Medicine. 2010;3:890-93. https://www.researchgate.net/publication/286915540
22. Oleszek WA. Chromatographic determination of plant saponins. Journal of Chromatography A. 2002;967:147–62. https://doi.org/10.1016/s0021-9673(01)01556-4
23. In-Seon P, Eun MK, Namsoo K. High-Performance Liquid Chromatographic Analysis of Saponin Compounds in Bupleurum falcatum. Journal of Chromatographic Science. 2000;38. https://doi.org/10.1093/chromsci/38.6.229
24. Ankur G, Surabhi M, Rajendra S. Evaluation of antimicrobial activity of Curcuma longa rhizome extract against Staphylococcus aureus. Biotechnology Reports. 2015;6:51-55.https://doi.org/10.1016/j.btre.2015.02.001
25. Meena DS, Pavithra MKS. Antifungal activity by ethanolic extracts of medicinal plants against Malassezia furfur: A potential application in the treatment of Dandruff. International Journal of Pharmtech Research. 2015;8:440-43. http://sphinxsai.com/2015/ph_vol8_no3/3/(440-443)V8N3.pdf
26. Liu H, Crawford JH, Pierce RB, Norris P, Platnick SE, Chen G, et al. Radiative effect of clouds on tropospheric chemistry in a global three-dimensional chemical transport model. Journal of Geophysical Research. 2006;111:D20303. https://doi.org/10.1029/2005JD006403
27. Innocent CO, Chinelo AE. Phytochemical screening and in vitro antimicrobial activity of various parts of Cleome ciliata Schum. & Thonn. International Journal of Research Sstudies in Biosciences. 2019;11:2018. https://academic-oup-com.eres.qnl.qa/biohorizons/article/doi/10.1093/biohorizons/hzy018/5304541
28. Sagar DR, Kelly S, Millns PJ, O’Shaughnessey CT, Kendall DA, Chapman V. Inhibitory effects of CB1 and CB2 receptor agonists on responses of DRG neurons and dorsal horn neurons in neuropathic rats. European Journal of Neurosciences. 2005;22:371–79. https://doi.org/10.1111/j.1460-9568.2005.04206.x
29. Khan MI, Ahhmed A, Shin JH, Baek JS, Kim MY, Kim JD. Green tea seed isolated saponins exerts antibacterial effects against various strains of gram positive and gram negative bacteria, a comprehensive study in vitro and in vivo. Evidence Based Complementary and Alternative Medicine. 2018;2018:12. https://doi.org/10.1155/2018/3486106
30. Murugan T, Albino JW, Murugan M. Antimicrobial activity and phytochemical constituents of leaf extracts of Cassia auriculata. Indian Journal of Pharmaceutical Sciences. 2013;75:122–25. https://doi.org/10.4103/0250-474X.113546
Downloads
Published
31-12-2019
How to Cite
1.
Chaudhary M, Verma V, Srivastava N. In Vitro Antiacne and Antidandruff activity of extracted stigmasterol from seed waste of safflower (Carthamus tinctorius L.). Plant Sci. Today [Internet]. 2019 Dec. 31 [cited 2024 Nov. 21];6(sp1):568-74. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/670
Issue
Section
Research Articles
License
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
