Enhancement of lipase production by ethyl methane sulfonate mutagenesis of soil fungal isolate
DOI:
https://doi.org/10.14719/pst.2019.6.sp1.674Keywords:
Ethyl methane sulfonate, fermentation processes, spores, Submerged fermentation, Solid State Fermentation, mutagenesisAbstract
Strain improvement through random mutagenesis is an extremely developed practice and it plays an important role in the economic growth of microbial agitation processes. The present study comprises of genetic improvement of fungus isolated from petrol pump soil by ethyl methane sulfonate (EMS) mutagenesis for increased production of extracellular lipase. Random mutagenesis was performed by incubating the spore suspension of fungus with EMS at a concentration of 5% (v/v) and 8% (v/v) for 30, 60 and 90 min, respectively. Control set was prepared by incubating the spore suspension with sterile distilled water. Control plate showed maximum number of fungal colonies whereas number of colonies was decreased as we increased exposure time of EMS from 30 to 90 min. The lipase activity of six mutagenic strains and wild strain was determined under submerged fermentation and solid state fermentation. Treated culture named as EMS5%-60min (obtained after 60 min exposure with 5% EMS) exhibited maximum activity (32.09 ± 1.84 IU/ml/min) in SmF as compared to wild strain (8.77 ± 3.52 IU/ml/min) and another treated strain named as EMS8%-90min (obtained after 90 min exposure with 8% EMS) exhibited maximum activity (7.99 ± 0.12 IU/g/min) in SSF as compared to wild strain (1.77 ± 0.71 IU/g/min). The activity of mutagenic strain i.e. EMS5%-60min was increased to 365.90% as compared to 100% activity of wild strain in SmF whereas activity of another mutagenic strain i.e. EMS8%-90min was increased to 451.41% as compared to 100% activity of wild strain in SSF.
Downloads
References
2. Devi SC, Mohanasrinivasan V, Jemimah Naine S, Yamini B, Chitra M, Nandhini G. Strain Improvement of Pseudomonas sp.VITSDVM1 for Optimization of Lipase Production by Chemical Mutagens. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2015; 6(3):782-87
3. Sharma AK, Sharma V, Saxena J, Kuila A. Lipase production from a wild (LPF-5) and a mutant (HN1) strain of Aspergillus niger. African Journal of Biotechnology. 2016;15(41):2292-2300. https://doi.org/10.5897/AJB2016.15600
4. Kumar RJ, Ilyas MMH. Strain improvement of Phosphate solubilizing fungal strains for the production of lipase. International Multidisciplinary Research Journal. 2011;1(7):15-23
5. Singh R, Kumar M, Mittal A, Mehta PK. Microbial enzymes: industrial progress in 21st century. 3 Biotech. 2016;6(2):174.https://doi.org/10.1007%2Fs13205-016-0485-8
6. Sarmah N, Revathi D, Sheelu G, Yamuna Rani K, Sridhar S, Mehtab V, Sumana C. Recent advances on sources and industrial applications of lipases. Biotechnology Progress. 2017;34(1):5-28. https://doi.org/10.1002/btpr.2581
7. Mehta A, Bodh U, Gupta R. Fungal Lipases: a review. Journal of Biotech Research. 2017;8:58-77
8. Andualema B, Gessesse A. Microbial Lipases and Their Industrial Applications: Review. Biotechnology. 2012;11(3):100-18. https://doi.org/10.3923/biotech.2012.100.118
9. Shaini VP, Jayasree S. Isolation and Characterization of Lipase Producing Bacteria from Windrow Compost. International Journal of Current Microbiology and Applied Sciences. 2016;5(5):926-33. https://doi.org/10.20546/ijcmas.2016.505.097
10. Streit WR, Schmitz RA. Metagenomics-the key to the uncultured microbes. Current Opinion in Biotechnology. 2004;7(5):492-98. https://doi.org/10.1016/j.mib.2004.08.002
11. Daniel R. The metagenomics of soil. Nature Reviews Microbiology. 2005;3(6):470–78. https://doi.org/10.1038/nrmicro1160
12. Lorenz P, Eck J. Metagenomics and industrial applications. Nature Reviews Microbiology. 2005;3(6):510–16. https://doi.org/10.1038/nrmicro1161
13. Bondkly AMEL, Keera AA. UV- and EMS- induced mutations affecting synthesis of alkaloids and lipase in Penicillium roquefortii. Arab Journal of Biotechnology. 2007;10(2):241-48
14. Adrio JL, Demain AL. Recombinant organisms for production of industrial products. Bioengineered Bugs. 2010;1(2):116-31. https://doi.org/10.4161%2Fbbug.1.2.10484
15. Bapiraju KVVSN, Sujatha P, Ramana T. Mutation induced enhanced biosynthesis of lipase. African Journal of Biotechnology. 2004;3(11):618-21
16. Gonzalez JB, Gernandez FJF, Tomasini A. Microbial secondary metabolites production and strain improvement. Indian Journal of Biotechnology. 2003;2(3):322-33
17. Karanam SK, Medicherla NR. Enhanced lipase production by mutation induced Aspergillus japonicas. African Journal of Biotechnology 2008;7(12):2064-67. https://doi.org/10.5897/AJB2008.000-5054
18. Prabakaran M, Thennarasu V, Ayeswariya Mangala R, Bharathidasan R, Chandrakala N, Mohan N. Comparative studies on the enzyme activities of wild and mutant fungal strains isolated from sugarcane field. Indian Journal of Science and Technology. 2009;2(11):46-49.
19. Joshi GK, Kumar S, Tripathi BN, Sharma V. Production of alkaline lipase by Corynebacterium paurometabolum MTCC6841 isolated from Lake Naukuchiatal, Uttaranchal State, India. Current Microbiology. 2006;52(5):354-58. https://doi.org/10.1007/s00284-005-0224-6
20. Lowry OH, Rosenbrough NJ, Farr AL, Randall A. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry. 1951; 93:265-75
21. Elliaiah P, Prabhakar T, Ramakrishna B, Taleb TA, Adinarayana K. Strain improvement of Aspergillus niger for the production of lipase. Indian Journal of Microbiology. 2002;42(2):151-53
22. Toscano L, Gochev V, Montero G, Stoytcheva M. Enhanced production of extracellular lipase by novel mutant strain of Aspergillus niger. Biotechnology & Biotechnological Equipment. 2011;25(1):2243-47. https://doi.org/10.5504/BBEQ.2011.0019
23. Colla LM, Primaz AL, Benedetti S, Loss RA, Lima MD, Reinehr CO, et al. Surface response methodology for the optimization of lipase production under submerged fermentation by filamentous fungi. Brazilian Journal of Microbiology. 2016;47(2):461-67. http://dx.doi.org/10.1016/j.bjm.2016.01.028
24. Ribeiro O, Magalhães F, Aguiar TQ, Wiebe MG, Penttilä M, Domingues L. Random and direct mutagenesis to enhance protein secretion in Ashbya gossypii. Bioengineered. 2013;4(5):322-31. https://doi.org/10.4161/bioe.24653
25. Radha S, Babu RH, Sridevi A, Prasad NBL, Narasimha G. Development of mutant fungal strains of Aspergillus niger for enhanced production of acid protease in submerged and solid state fermentation. European Journal of Experimental Biology. 2012;2:1517-28
26. Rajeshkumar J, Ilyas MHM. Strain improvement of Phosphate solubilizing fungal strains for the production of lipase. International Multidisciplinary Research Journal 2011;1(7):15-23
27. Sikora P, Chawade A, Larsson M, Olsson J, Olsson O. Mutagenesis as a Tool in Plant Genetics, Functional Genomics, and Breeding. International Journal of Plant Genomics. 2011;1-13. http://dx.doi.org/10.1155/2011/314829
28. Jayaraman R, Ilyas MHM. Strain Improvement of Phosphate Solubilizing Fungal Strains. Journal of Ecobiotechnology. 2010;2:65-70
29. Rajan A, Nair AJ. A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate. Turkish Journal of Biology 2011;35(5):569-574. https://doi.org/10.3906/biy-0912-6
30. Yousaf M, Irfan M, Khokhar ZU, Syed QA, Baig S, Iqbal A. Enhanced Production of Protease by Mutagenized Strain of Aspergillus oryzae in Solid Substrate Fermentation Of Rice Bran. Science International. 2011; 22(2):119-23
31. Freifelder D. Microbial Genetics. Narosa Publishing House. New Delhi, India. 1990; 191-210
32. Radman M. Enzyme of evolutionary change. Nature 1999; 401:866-68. https://doi.org/10.1038/44738
33. Farahbakhsh A, Ghasemi M, Ataie SE. Using Fermentation Processes For Production of Lipase by Aspergillus niger, 2nd International Conference on Environment, Agriculture and Food Sciences, August 25-26, Kuala Lumpur (Malayia); 2013.
34. Kumar A, Kanwar SS. Lipase Production in Solid-State Fermentation (SSF): Recent Developments and Biotechnological Applications. Dynamic Biochemistry, Process Biotechnology and Molecular Biology. 2012;6(1):13-27.
Downloads
Published
How to Cite
Issue
Section
License
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
