Skip to main navigation menu Skip to main content Skip to site footer
Plant Science Today (PST)

Research Articles

Early Access

Exploring the potential of saline-tolerant Streptomyces for managing blast disease in rice (Oryza sativa L.)

DOI
https://doi.org/10.14719/pst.6901
Submitted
25 December 2024
Published
21-08-2025
Versions

Abstract

Rice (Oryza sativa L.) is recognized as one of the world’s most important staple food crops. Yield loss due to diseases is one of the major constraints in rice cultivation. Among them, the blast disease caused by Magnaporthe oryzae ranks first in affecting the rice crop. Hence, the present study proposed to study the efficacy of antagonistic activities of Streptomyces spp. against blast disease in rice. Twenty isolates of the antagonistic Streptomyces sp. were isolated from rhizosphere soil. From the sequence analysis of 16s rRNA of isolate TRYS 10 was identified as Streptomyces caelestis. Antagonistic activity assay was conducted using a dual culture technique for blast pathogens. The isolates S. caelestis (TRYS 10) and TRYS 16 were effective against blast and recorded the inhibition zone of 19 and 15.2 mm, respectively followed by TRYS 5 (9.0 mm).  An isolate S. caelestis (TRYS 10) induced the growth promotion of rice viz, root length (16.72 cm), shoot length (22.13 cm) and vigour index (3807). S. caelestis (TRYS 10) was tested for its efficacy against blasts under greenhouse conditions. Disease severity of blast in pathogen-inoculated control plants was 8.89 %, while in Streptomyces + pathogen-inoculated, it was 2.22 %. A foliar spray of S. caelestis (TRYS 10) at the time of symptom appearance and repeated 15 days later was found to be effective in reducing blast in rice up to 42.64 % under field conditions. Two sprays of S. caelestis at the time of initial appearance of blast disease and 15 days later reduced the disease incidence as well as improved the plant growth and yield in rice.

References

  1. 1. Ashkani S, Yusop MR, Shabanimofrad M, Haron AR, Sahebi M, Latif MA. Genetic analysis of resistance to rice blast: A study on the inheritance of resistance to the blast disease pathogen in an F3 population of rice. J Phytopathol. 2015;163:300–09. https://doi.org/10.1111/jph.12323
  2. 2. Asibi AE, Chai Q, Coulter JA. Rice blast: A disease with implications for global food security. Agronomy. 2019;9:451. https://doi.org/10.3390/agronomy9080451
  3. 3. Kato H. Rice blast disease. Pestic Outlook. 2001;12:23–25. https://doi.org/10.1039/b100803j
  4. 4. Pooja K, Katoch A. Past, present and future of rice blast management. Plant Sci Today. 2014;1:165–73. https://doi.org/10.14719/pst.2014.1.3.24
  5. 5. Newitt JT, Prudence SMM, Hutchings MI, Worsley SF. Biocontrol of cereal crop diseases using Streptomycetes. Pathogens. 2019;8:78. https://doi.org/10.3390/pathogens8020078
  6. 6. Law JW, Ser HL, Khan TM, Chuah LH, Pusparajah P, Chan KG, et al. The potential of Streptomyces as biocontrol agents against the rice blast fungus, Magnaporthe oryzae (Pyricularia oryzae). Front Microbiol. 2017;8:1–10. https://doi.org/10.3389/fmicb.2017.00003
  7. 7. Awla HK, Kadir J, Othman R, Rashid TS, Hamid S, Wong MY. Plant growth-promoting abilities and biocontrol efficacy of Streptomyces sp. UPMRS4 against Pyricularia oryzae. Biol Control. 2017;112:55– 63. https://doi.org/10.
  8. 1016/j.biocontrol.2017.05.011
  9. 8. Ningthoujam S, Sanasam S, Tamreihao K, Nimaich S. Antagonistic activities of local actinomycete isolates against rice fungal pathogens. Afr J Microbiol Res. 2009;3:737–42.
  10. 9. Boukaew S, Prasertsan P. Suppression of rice sheath blight disease using a heat-stable culture filtrate from Streptomyces philanthi RM-1-138. Crop Prot. 2014;61:1–10. https://doi.org/10.1016/j.cropro.2014.02.012
  11. 10. Xu T, Cao L, Zeng J, Franco CMM, Yang Y, Hu X, et al. The antifungal action mode of the rice endophyte Streptomyces hygroscopicus OsiSh-2 as a potential biocontrol agent against the rice blast pathogen. Pestic Biochem Phys. 2019;160:58–69. https://doi.org/10.1016/j.pestbp.2019.06.015
  12. 11. Chaiharn M, Theantana T, Pathom-aree W. Evaluation of biocontrol activities of Streptomyces spp. against rice blast disease fungi. Pathogens. 2020;126(9):1–16. https://doi.org/10.3390/pathogens9020126
  13. 12. Vincent JM. Distortion of fungal hyphae in the presence of certain inhibitors. Nature. 1927;59:850. https://doi.org/
  14. 10.1038/159850b0
  15. 13. Inderiati S, Franco CM. Isolation and identification of endophytic actinomycetes and their antifungal activity. J Biotechnol Res Trop Reg. 2008;1:1–6. https://api.semanticscholar.org/CorpusID:88925597
  16. 14. International Rice Research Institute. Standards Evaluation System for Rice; IRRI: Manila, Philippines, 2013.
  17. 15. Li Q, Jiang Y, Ning P, Zheng L, Huang J, Li G. Suppression of Magnaporthe oryzae by culture filtrates of Streptomyces globisporus JK-1. Biol Control. 2011;58:139–48. https://doi.org/10.1016/j.biocontrol.2011.04.013
  18. 16. Zarandi ME, Bonjar GHS, Dehkaei FP. In vitro antagonistic antifungal-activity of Streptomyces isolate 339 against Magnaporthe oryzae. Am J Agric Biol Sci. 2013;8(3):212–6. https://doi.org/10.3844/ajabssp.2013.212.216
  19. 17. Khalil MS, Moubasher H, Hasan FF. Biological control of rice blast disease by Streptomyces flavotricini. Res J Pharm Biol Chem Sci. 2014;5:1453–61. https://api.semanticscholar.org/CorpusID:85633668
  20. 18. Chaiharn C, Sujada N, Pathom-aree W, Lumyong S. Biological control of Rigidoporus microporus the casue of white root disease in rubber, using PGPRs in vivo. Chiang Mai J Sci. 2019;46:850–66. https://epg.science.cmu.ac.th/ejournal
  21. 19. Zarandi ME, Bonjar GS, Dehkaei FP, Moosavi SA, Farokhi PR, Aghighi S. Biological control of rice blast (Magnaporthe oryzae) by use of Streptomyces sindeneusis isolate 263 in greenhouse. Am J Appl Sci. 2009;6:194–99. https://doi.org/10.3844/ajas.2009.194.199
  22. 20. Ahsan T, Liu H, Shan YH, Zhou T, Ahmed M, Li B, et al. Identification and bio-control activity of Streptomyces strain (Koyanogensis) against Magnaporthe grisea. Biotechnol Equip. 2021;35(1):1891–98. https://doi.org/10.1080/1310
  23. 2818.2022.2026816
  24. 21. Soltani Nejad M, Samandari Najafabadi N, Aghighi S, Shahidi Bonjar AH, Murtazova KM-S, Nakhaev MR, et al. Investigating the potential of Streptomyces spp. in suppression of Rhizoctonia solani (AG1-IA) causing rice sheath blight disease in Northern Iran. Agronomy. 2022;12(10):2292. https://doi.org/10.3390/agronomy12102292

Downloads

Download data is not yet available.