Isolation and characterization of Rhizobium sp. from a collar rot tolerant groundnut (Arachis hypogaea L.) variety

Authors

  • Shalvi Pandey Department of Bioscience and Biotechnolgy, Banasthali Vidyapith, Rajsthan 304022, India
  • Dipjyoti Chakraborty Department of Bioscience and Biotechnolgy, Banasthali Vidyapith, Rajsthan 304022, India

DOI:

https://doi.org/10.14719/pst.2019.6.sp1.693

Keywords:

Rhizobium pongamiae, crop legume, collar rot, Aspergillus niger, Arachis hypogaea

Abstract

Groundnut, a major pulse crop, is prone to fungal diseases especially collar rot at seed germination and early seedling stage. In the present study, symbiotic bacteria was isolated and characterised from a collar rot resistant groundnut variety, Mallika and identified as Rhizobium pongamiae following biochemical and molecular characterization (NCBI Acc. No. MK770416). The bacterial isolate on cross inoculation, may be useful in improving crop productivity and enhancing collar rot disease tolerance in susceptible crops.

Downloads

Download data is not yet available.

References

1. Sharma P, Kumar Saini M, Deep S, Kumar V. Biological control of groundnut root rot in farmer’s field. J Agric Sci. 2012;4(8). https://doi.org/10.5539/jas.v4n8p48

2. Ayoola PB, Adeyeye A, Onawumi OO. Chemical evaluation of food value of groundnut (Arachis hypogaea) seeds. Am J Food Nutr. 2012;2(3):55-57. https://doi.org/10.5251/ajfn.2012.2.3.55.57

3. Hayat R, Ahmed I, Sheirdil RA. An overview of plant growth promoting rhizobacteria (PGPR) for sustainable agriculture. In: Ashraf M, Öztürk M, Ahmad M, Aksoy A (eds) Crop Production for Agricultural Improvement. Springer, Dordrecht; 2012, pp. 557–79.https://doi.org/10.1007/978-94-007-4116-4_22

4. Gajera HP, Vakharia DN. Production of lytic enzymes by Trichoderma isolates during in vitro antagonism with Aspergillus niger, the causal agent of collar rot of peanut. Braz J Microbiol. 2012;43:43-52. https://doi.org/10.1590/S1517-83822012000100005

5. Datta A, Singh RK, Kumar S. Isolation, characterization and growth of Rhizobium strains under optimum conditions for effective biofertilizer production. Int J Pharm Sci Rev Res. 2015;32(1):199-208. ISSN 0976 – 044X.

6. Mosharof HAKM, Musharraf HM, Hakim MA, Islam MM, Jannatul F. Isolation and selection of Bradyrhizobium from the root nodules of indigo plants (Indigofera tinctoria L.). Afr J Biotechnol. 2012;11(58):12183-91. https://doi.org/10.5897/AJB12.1288

7. Pawar VA, Pawar PR, Bhosale AM, Chavan SV. Effect of Rhizobium on seed germination and growth of plants. J Acad In Res. 2014;3(2):84-88. ISSN: 2278-5213.

8. Saraf R, Chaudhary S, Chakraborty D. Isolation and characterization of osmotolerant Rhizobium sp. from Vigna mungo (L.) Hepper grown in semi-arid region. Vegetos. 2018;31:102-07. https://doi.org/10.5958/2229-4473.2018.00039.3

9. Bernaerts MJ, Deley J. A biochemical test for crown gall bacteria. Nature. 1963;197:406-07. https://doi.org/10.1038/197406b0

10. Kamal J, Bano A. Potential allelopathic effects of sunflowers on microorganisms. Afr J Biotechnol. 2008;7(22):4208-11. https://doi.org/10.5897/AJB08.837

11. Gachande BD, Khansole GS. Morphological, cultural and biochemical characteristics of Rhizobium japonicum and Bradyrhizobium japoicum of soybean. Biosci Discov. 2011; 2:1-4. ISSN 2229-3469.

12. Weisburg WG, Barns SM, Pelletie DA, Lane DJ. 16S rDNA amplification for phylogenetic study. J Bacteriol. 1991; 173:697-703. https://doi.org/10.1128/jb.173.2.697-703.1991

13. Singh BK, Nazaries L, Munro S, Anderson IC, Campbell CD. Use of multiplex terminal restriction fragment length polymorphism for rapid and simultaneous analysis of different components of the soil microbial community. Appl Environ Microbiol. 2006;72:7278-85. https://doi.org/10.1128/AEM.00510-06

14. Kumari D, Chakraborty D. Drought stress mitigation in Vigna radiata by the application of root-nodulating bacteria. Plant Sci Today. 2017;4(4);209-12. https://doi.org/10.14719/pst.2017.4.4.343

15. Pandey S, Ror S, Chakraborty, D. Analysis of Biochemical Responses in Vigna mungo varieties subjected to drought stress and possible amelioration. Int J Sci Resh Agric Sci. 2014;1:6-15.https://doi.org/10.12983/ijsras-2014-p0006-0015

16. Chaudhary S, Chakraborty D. Cross inoculation with beneficial Rhizobium strain promotes plant growth in Vigna mungo. Vegetos. 2019;32(2):223. https://doi.org/10.1007/s42535-019-00026-3

Downloads

Published

31-12-2019

How to Cite

1.
Pandey S, Chakraborty D. Isolation and characterization of Rhizobium sp. from a collar rot tolerant groundnut (Arachis hypogaea L.) variety. Plant Sci. Today [Internet]. 2019 Dec. 31 [cited 2024 Nov. 24];6(sp1):631-4. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/693

Issue

Section

Research Articles