Biochemical response of three Vigna mungo varieties (T9, RBU38 and VM4) under drought stress
DOI:
https://doi.org/10.14719/pst.2015.2.2.99Keywords:
Vigna mungo, drought stress, ROSAbstract
Plants apply several strategies that are developed during their evolution and artificial domestication to overcome biotic and abiotic stresses. Among eminent environmental threats drought stress is a major factor that affects plants at physiological, biochemical and molecular level. Blackgram (Vigna mungo) is an important pulse crop but its productivity is adversely affected by drought. In the present work, different cultivars of blackgram i.e. T9, RBU38 and VM4 are taken to find out the effects of drought stress by the estimation of different biochemical parameters to better understand biochemical pathway modulations under stress and its possible mitigation. Damage to photosynthetic machinery as evident by decrease in chlorophyll content and loss of membrane integrity in the plants under drought stress. The adverse effects of drought on the plants were averted to a certain extent in RBU38 by activation of defence signalling through H2O2 at lower concentration, which proved damaging at high concentration for T9 and VM4 and a concurrent increase in proline content which may provide protection against oxidative stress. This study suggests that drought modulated biochemical parameters can be used as reliable indices for selection of genotypes with a better stress tolerance.
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Alam I, Sharmin SA, Kim KH, Yang JK, Choi MS, Lee BH (2010). Proteome analysis of soybean roots subjected to short-term drought stress. Plant Soil. 333:491–505. doi: 10.1007/s11104-010-0365-7
Alam MM, Hasanuzzaman M, Nahar K, Fujita M (2013). Exogenous salicylic acid ameliorates short-term drought stress in mustard (Brassica juncea L.) seedlings by up-regulating the antioxidant defense and glyoxalase system. Australian Journal of Crop Science. 7(7):1053-1063.
Alexieva V, Sergiev I, Mapellis S, Karanov E (2001). The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant Cell & Environment. 24:1337–1344. doi: 10.1046/j.1365-3040.2001.00778.x
Allen DJ, Ort DR (2001). Impact of chilling temperatures on photosynthesis in warm climate plants. Trends in Plant Science. 6:36-42. doi: 10.1016/S1360-1385(00)01808-2
Alpert P, Oliver MJ (2002). Drying without dying. In: Black M, Pritchard HW (eds) Desiccation and survival in plants: drying without dying. CABI publishing, Oxford and New York, pp 1-45. doi: 10.1079/9780851995342.0003
Apel K, Heribert H (2004). Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology. 55:373-399. doi: 10.1146/annurev.arplant.55.031903.141701 PMid:15377225
Arnon DI (1949). Copper enzymes in isolated chloroplasts: polyphenol oxidases in Beta vulgaris. Plant Physiology. 24:1-15. doi: 10.1104/pp.24.1.1 PMid:16654194 PMCid:PMC437905
Arulbalachandran D, Mullainathan L, Velu S, Thilagavathi C (2010). Genetic variability, heritability and genetic advance of quantitative traits in black gram by effects of mutation in field trail. African Journal of Biotechnology. 9(19): 2731-2735.
Baisak R, Rana D, Acharya PBB, Kar M (1994). Alterations in the activities of active oxygen scavenging enzymes of wheat leaves subjected to water stress. Plant Cell and Physiology. 35:489-495.
Bates LS, Waldren RP, Teare ID (1973). Rapid determination of free proline for water-stress studies. Plant Soil. 39:205-207. doi: 10.1007/BF00018060
Borade VP, Kadam, S.S. and Salunkhe, D.K. 1984. Changes in phytate, phosphorus and minerals during germination and cooking of horse gram and moth bean. Plant Foods for Human Nutrition. 34(2):151-157. doi: 10.1007/BF01094843
Bray EA (1997). Plant responses to water deficit. Trends in Plant Science. 2(2):48-54. doi: 10.1016/S1360-1385(97)82562-9
Chakraborty D, Sircar D, Mitra A (2008). Phenylalanine ammonia-lyase- mediated biosynthesis of 2–hydroxy–4- methoxy benzaldehyde in roots of Hemidesmus indicus. Journal of Plant Physiology. 165(10):1033-1040. doi: 10.1016/j.jplph.2007.09.002 PMid:18023917
De Vos, CHR, Schat H, Vooijs R, Ernst WHO (1989). Copper-induced damage to the permeability barrier in roots of silene cucubalus. Plant Physiology. 135:164-179. doi: 10.1016/S0176-1617(89)80171-3
Dubey RS, Pessarakli M (2002). Physiological mechanisms of nitrogen absorption and assimilation in plants under stressful conditions. In: Pessarakli M. (ed.), Handbook of Plant and Crop Physiology. Marcel Dekker, New York, pp. 637–655.
Frary A, Gol D, Keles D, Okmen B, Pinar H, Sigva HO, et al. 2010. Salt tolerance in Solanum pennellii: antioxidant response and related QTL. BMC Plant Biology. 10:58. doi: 10.1186/1471-2229-10-58 PMid:20370910 PMCid:PMC2923532
Gapinska M, Sklodowska M, Gabara B (2008). Effect of short and long term salinity on the activities of antioxidative enzymes and lipid peroxidation in tomato roots. Acta Physiologiae Plantarum. 30:11-18. doi: 10.1007/s11738-007-0072-z
He L, Jia X, Gao Z, Li R (2011). Genotype-dependent responses of wheat (Triticum aestivum L.) seedlings to drought, UV-B radiation and their combined stresses. African Journal of Biotechnology. 10(20):4046-56.
Jha P, Jindal R, Chakraborty D (2013). HPLC quantification of phenolic acids from Vetiveria zizanioides (Linn.) Nash. and its antioxidant and antimicrobial activity. Journal of Pharmaceutics. 2013:1-6. Article ID 270472.
Keyvan S (2010). The effects of drought stress on yield, relative water content, proline, soluble carbohydrates and chlorophyll of bread wheat cultivars. Journal of Animal & Plant Sciences. 8(3):1051-1060.
Khan NA, Samiullah, Singh S, Nazar R (2007). Activities of antioxidative enzymes, sulphur assimilation, photosynthetic activity and growth of wheat (Triticum aestivum) cultivars differing in yield potential under cadmium stress. Journal of Agronomy and Crop Science. 193:435-444. doi: 10.1111/j.1439-037X.2007.00272.x
Kundu S, Chakraborty D, Pal A (2011) Proteomic analysis of salicylic acid induced resistance to Mungbean Yellow Mosaic India Virus in Vigna mungo. Journal of Proteomics. 74(3): 337–349. doi: 10.1016/j.jprot.2010.11.012 PMid:21130907
Madamanchi NR, Donahue JL, Cramer CL, Alscher RG, Pedersen K (1994). Differential response of Cu Zn superoxide dismutases in two pea cultivars during a short term exposure to sulphur dioxide. Plant Molecular Biology. 26:95-103. doi: 10.1007/BF00039523 PMid:7948909
Mandal SM, Chakraborty D, Dey S (2010). Phenolic acids are the signaling molecule in plant-microbe symbioses. Plant Signalling & Behavior. 5(4):359-368. doi: 10.4161/psb.5.4.10871 PMid:20400851 PMCid:PMC2958585
Pandey S, Ror S, Chakraborty D (2014). Analysis of biochemical responses in Vigna mungo varieties subjected to drought stress and possible amelioration. International Journal of Scientific Research in Agricultural Sciences. 1(1):6-15. doi: 10.12983/ijsras-2014-p0006-0015
Quanet L, Zang B, Shi W, Li H (2008). Hydrogen peroxide in plants: a versatile molecule of the reactive oxygen species network. Journal of Integrative Plant Biology. 50:2-18. doi: 10.1111/j.1744-7909.2007.00599.x PMid:18666947
Saravanakumar D, Harish S, Loganathan M, Vivekananthan R, Rajendran L, Raguchander T, Samiyappan R (2007). Rhizobacterial bioformulation for the effective management of Macrophomina root rot in mungbean. Archives of Phytopathology and Plant Protection. 40(5):323–337. doi: 10.1080/03235400600587326
Singleton VL, Orthofer R, Lamuela-Raventos RM (1999). Analysis of total phenol and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. Methods Enzymology. 299:152–78. doi: 10.1016/S0076-6879(99)99017-1
Smirnoff N (1993). The role of active oxygen in the response of plants to water deficit and desiccation. New Phytologist. 125:27–58. doi: 10.1111/j.1469-8137.1993.tb03863.x
Souframanien J, Gopalakrishna T (2006). ISSR and SCAR markers linked to the mungbean yellow mosaic virus (MYMV) resistance gene in blackgram [Vigna mungo (L.) Hepper]. Plant Breeding. 125:619–622. doi: 10.1111/j.1439-0523.2006.01260.x
Trippi VS, Gidrol X, Pradet A (1989). Effects of oxidative stress by oxygen and hydrogen peroxide on energy metabolism and senescence in oat leaves. Plant Cell Physiology. 30:210–217.
Turkan I, Bor M, Ozdemir F, Koca H (2005). Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Science. 168:223–231. doi: 10.1016/j.plantsci.2004.07.032
Walters C, Farrant JM, Pammenter NW, Berjak P (2002). Desiccation stress and damage. In: Black M, Pritchard HW (eds) Desiccation and survival in plants: drying without dying. CABI publishing, Oxford and New York, pp 263-293. doi: 10.1079/9780851995342.0263
Wang J, Li DQ, Gu LS (2002). The response to water stress of the antioxidant system in maize seedling roots with different drought resistance. Acta Botanica Boreali-Occidentalia Sinica. 22:285-290.
Xiangwen X, Fan Y, Sheng Z, Helena K, Chunyang L (2009). Physiological and proteomic responses of two contrasting Populus cathayana populations to drought stress. Physiologia Plantarum. 136:150–168. doi: 10.1111/j.1399-3054.2009.01222.x PMid:19453505
Yazdanpanah, Baghizadeh A, Abbassi F (2011). The interaction between drought stress and salicylic and ascorbic acids on some biochemical characteristics of Satureja hortensis. African Journal of Agricultural Research. 6(4):798-807.
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