Plants apply several strategies that are developed during their evolution and artificial domestication to overcome biotic and abiotic stresses. Among eminent environmental threats drought stress is a major factor that affects plants at physiological, biochemical and molecular level. Blackgram (Vigna mungo) is an important pulse crop but its productivity is adversely affected by drought. In the present work, different cultivars of blackgram i.e. T9, RBU38 and VM4 are taken to find out the effects of drought stress by the estimation of different biochemical parameters to better understand biochemical pathway modulations under stress and its possible mitigation. Damage to photosynthetic machinery as evident by decrease in chlorophyll content and loss of membrane integrity in the plants under drought stress. The adverse effects of drought on the plants were averted to a certain extent in RBU38 by activation of defence signalling through H2O2 at lower concentration, which proved damaging at high concentration for T9 and VM4 and a concurrent increase in proline content which may provide protection against oxidative stress. This study suggests that drought modulated biochemical parameters can be used as reliable indices for selection of genotypes with a better stress tolerance.