The optimal row patterns in maize-soybean intercropping enhance resource use efficiency, land productivity and profitability, promoting sustainability in resource-limited regions. The experiment was conducted at the CAU Research Farm in Imphal, Manipur, during the kharif season of 2022-23. Seven treatments were tested, including sole soybean, sole maize and five maize-soybean intercropping combinations (2:2, 3:1, 3:2, 4:1, 4:2), with four replications each. The results showed that sole crops performed better than intercropped treatments in most growth and yield parameters. Among intercropping treatments, 3S:1M (three rows of soybean to one row of maize) configuration achieved the highest seed yield, with 775 kg ha-1 for soybean and 4006 kg ha-1 for maize. The correlation analysis indicated strong associations between soybean grain yield with dry matter accumulation (r = 0.948) and the number of pods per plant (r = 0.944). In contrast, maize yield was linked to the crop growth rate (r = 0.957) and the number of cobs per plant (r = 0.924). Principal component analysis (PCA) identified biomass and pod production as the primary contributors to yield variation in soybean and dry matter accumulation and crop growth rate for maize. One row of maize intercropped between three rows of soybean showed the highest land equivalent ratio (1.44), indicating a yield advantage over sole cropping, alongside the highest economic performance with a net return of ₹103123 and a benefit-cost ratio of 3.21. Maize exhibited dominance over soybean in intercropping, with an aggressivity index of 0.55. These findings suggest that one row of maize intercropped between three rows of soybean intercropping patterns optimizes productivity and profitability, providing a sustainable solution for smallholder farmers.