Expression study of an Amino Acid Permease-like gene in Phaseolus vulgaris L.
DOI:
https://doi.org/10.14719/pst.2020.7.2.712Keywords:
Alanine, Amino acid permease-like gene, Glycine, Proline, Phaseolus vulgarisAbstract
Amino acid permease-like (AAP-like) gene plays a critical role in absorbing amino acids through roots in plants. A number of studies have been done on amino acids uptake in plants but till date there is no report about the expression of AAP gene in Phaseolus under field allied condition. The aim of this study is to measure the expression of AAP-like gene on alanine, glycine and proline amino acid uptake capacity in Phaseolus vulgaris at field relevant concentrations. Amongst three amino acids, a drastic significant increase of 63.15 fold in expression of AAP-like gene is observed in 50 µM alanine at 2 hr. At 50 µM of proline and 25 µM of alanine, AAP-like gene expression also shows high expression of 43.71 fold at 2 hr and 42.50 fold at 1 hr respectively. This study elucidated the dose dependent relationship of glycine, alanine and proline with the expression of AAP-like gene in amino acid transport in natural conditions in roots of P. vulgaris. Additionally, this research is also useful in identification of plants needing less surplus nitrogen additions and helpful in optimizing fertilizers by tailoring AAP gene expression to match plant uptake capacities in agriculture.
Downloads
Download data is not yet available.
References
1. Williams LE, Miller AJ. Transporters responsible for the uptake and partitioning of nitrogenous solutes. Annu Rev Plant Physiol Plant Mol Biol. 2001;52:659–88. https://doi.org/10.1146/annurev.arplant.52.1.659
2. Tegeder M, Rentsch D. Uptake and partitioning of amino acids and peptides. Mol Plant. 2010;3(6):997–1011. https://doi.org/10.1093/mp/ssq047
3. Rahmoune B, Zerrouk IZ, Bouzaa S, Morsli A, Khelifi-Slaoui M, Ludwig-Müller J, Khelifi L. Amino acids profiling in Datura stramonium and study of their variations after inoculation with plant growth promoting Rhizobacteria. Biologia. 2019;74:1373–83. https://doi.org/10.2478/s11756-019-00287-y
4. Tegeder M, Weber APM. Metabolite transporters in control of primary plant metabolism. In: Plaxton WC, McManus MT, editors. Control of Primary Metabolism in Plants. 2006;85–120. https://doi.org/10.1002/9780470988640.ch4
5. Rentsch D, Schmidt S, Tegeder M. Transporters for uptake and allocation of organic nitrogen compounds in plants. FEBS Letters. 2007;581:2281–89. https://doi.org/10.1016/j.febslet.2007.04.013
6. Tegeder M. Transporters for amino acids in plant cells: some functions and many unknowns. Curr Opin Plant Biol. 2012;15:315–21. https://doi.org/10.1016/j.pbi.2012.02.001
7. Fischer WN, Kwart M, Hummel S, Frommer WB. Substrate specificity and expression profile of amino acid transporters (AAPs) in Arabidopsis. J Biol Chem. 1995;270(27):16315–20. https://doi.org/10.1074/jbc.270.27.16315
8. Fischer WN, Loo DD, Koch W, Ludewig U, Boorer KJ, Tegeder M, et al. Low and high affinity amino acid H+-cotransporters for cellular import of neutral and charged amino acids. Plant J. 2002;29(6):717–31. https://doi.org/10.1046/j.1365-313X.2002.01248.x
9. Svennerstam H, Ganeteg U, Bellini C, Näsholm T. Root uptake of cationic amino acids by Arabidopsis depends on functional expression of amino acid permease. New Phytol. 2008;180:620–30. https://doi.org/10.1111/j.1469-8137.2008.02589.x
10. Schwacke R, Schneider A, Van Der Graaff E, Fischer K, Catoni E, Desimone M, et al. Aramemnon, a Novel Database for Arabidopsis Integral Membrane Proteins. Plant Physiol. 2003;131(1):16–26. https://doi.org/10.1104/pp.011577
11. Jamtgard S, Näsholm T, Huss-Danell K. Characteristics of amino acid uptake in barley. Plant Soil. 2008;302:221–31. https://doi.org/10.1007/s11104-007-9473-4
12. Svennerstam H, Jämtgard S, Ahmad I, Huss-Danel K, Näsholm T, Ganeteg U. Transporters in Arabidopsis roots mediating uptake of amino acids at naturally occurring concentrations. New Phytol. 2011;191:459–67. https://doi.org/10.1111/j.1469-8137.2011.03699.x
13. Nithin, C, Patwa N, Thomas A, Bahadur RP, Basak J. Computational prediction of miRNAs and their targets in Phaseolus vulgaris using simple sequence repeat signatures. BMC Plant Biol. 2015;15:1–16. https://doi.org/10.1186/s12870-015-0516-3
14. Patwa N, Nithin C, Bahadur RP, Basak J. Identification and characterization of differentially expressed Phaseolus vulgaris miRNAs and their targets during mungbean yellow mosaic India virus infection reveals new insight into Phaseolus-MYMIV interaction. Genomics. 2019;111:1333–42. https://doi.org/10.1016/j.ygeno.2018.09.005
15. Tan Q, Grennan AK, Pélissier HC, Rentsch D, Tegeder M. Characterization and expression of French bean amino acid transporter PvAAP1. Plant Sci. 2008;174:348–56. https://doi.org/10.1016/j.plantsci.2007.12.008
16. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962;15:473–97.https://doi.org/10.1111/j.1399-054.1962.tb08052.x
17. Artimo P, Jonnalagedda M, Arnold K, Baratin D, Csardi G, de Castro E, et al. ExPASy: SIB bioinformatics resource portal. Nucleic Acids Res, 2012;40(W1):W597–W603. https://doi.org/10.1093/nar/gks400
18. Koressaar T, Lepamets M, Kaplinski L, Raime K, Andreson R, Reme M. Primer3_masker: integrating masking of template sequence with primer design software. Bioinformatics. 2018;34(11):1937–38. https://doi.org/10.1093/bioinformatics/bty036
19. Borges A, Tsai SM, Caldas DGG. Validation of reference genes for RT-qPCR normalization in common bean during biotic and abiotic stresses. Plant Cell Rep 2012;31:827–38. https://doi.org/10.1007/s00299-011-1204-x
20. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative CT method. Nat Protoc. 2008;3:1101–08. https://doi.org/10.1038/nprot.2008.73
21. Jones DL, Owen AG, Farrar JF. Simple method to enable the high resolution determination of total free amino acids in soil solutions and soil extracts. Soil Biol Biochem. 2002;34:1893–02. https://doi.org/10.1016/S0038-0717(02)00203-1
22. Hill PW, Quilliam RS, DeLuca TH, Farrar J, Farrell M, Roberts P, et al. Acquisition and Assimilation of Nitrogen as Peptide-Bound and D-Enantiomers of Amino Acids by Wheat. PLoS One. 2011;6: e19220. https://doi.org/10.1371/journal.pone.0019220
23. Raab TK, Lipson DA, Monson RK. Non-mycorrhizal uptake of amino acids by roots of the alpine sedge Kobresia myosuroides: implications for the alpine nitrogen cycle. Oecologia.1996;108:488–94. https://doi.org/10.1007/BF00333725
24. Öhlund J. Organic and inorganic nitrogen sources for conifer seedlings: abundance, uptake and growth. Doctoral thesis. Swedish University of Agricultural Sciences. PhD [Dissertation]. Umea: Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences; 2004. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-278
25. Wright DE. Amino acid uptake by plant roots. Arch Biochem Biophys. 1962;97:174–180. https://doi.org/10.1016/0003-9861(62)90061-9
26. Schmidt S, Stewart GR. Glycine metabolism by plant roots and its occurrence in Australian plant communities. Aust J Plant Physiol. 1999;26:253–64. https://doi.org/10.1071/PP98116
27. Owen AG, Jones DL. Competition for amino acids between wheat roots and rhizosphere micro-organisms and the role of amino acids in plant N acquisition. Soil Biol Biochem. 2001;33:651–57. https://doi.org/10.1016/S0038-0717(00)00209-1
28. Soldal T, Nissen P. Multiphasic uptake of amino acids by barley roots. Physiol Plant. 1978;43:181–88. https://doi.org/10.1111/j.1399-3054.1978.tb02561.x
29. Schobert C, Komor E. Amino-acid-uptake by Ricinus communis roots - characterization and physiological significance. Plant Cell Environ. 1987;10:493–500. https://doi.org/10.1111/j.1365-3040.1987.tb01827.x
30. Kielland K. Amino acid absorption by arctic plants: Implications for plant nutrition and nitrogen cycling. Ecology. 1994;75(8):2373–83. https://doi.org/10.2307/1940891
31. Nasholm T, Ekblad A, Nordin A, Giesler R, Hogberg M, Hogberg P. Boreal forest plants take up organic nitrogen. Nature. 1998;392:914–16. https://doi.org/10.1038/31921
32. Harrison J, Brugiere N, Phillipson B, Ferrario-Mery S, Becker T, Limami A, Hirel B. Manipulating the pathway of ammonia assimilation though genetic engineering and breeding: consequences to plant physiology and plant development. Plant Soil. 2000;221:81–93. https://doi.org/10.1023/A:1004715720043
33. Persson J, Nasholm T. Regulation of amino acid uptake in conifers by exogenous and endogenous nitrogen. Planta. 2002;215:639–44. https://doi.org/10.1007/s00425-002-0786-5
34. Gioseffi E, Neergaard Ade, Schjoerring JK. Interactions between uptake of amino acids and inorganic nitrogen in wheat plants. Biogeosciences. 2012;9:1509–18. https://doi.org/10.5194/bg-9-1509–2012
35. Lipson DA, Nasholm T. The unexpected versatility of plants: organic nitrogen use and availability in terrestrial ecosystems. Oecologia. 2001;128:305–16. https://doi.org/10.1007/s004420100693
36. Chakraborty N, Besra A, Basak J. Molecular Cloning of an Amino Acid Permease Gene and Structural Characterization of the Protein in Common Bean (Phaseolus vulgaris L.). Mol Biotechnol. 2020;62. https://doi.org/10.1007/s12033-020-00240-4
37. Garneau MG, Tan Q, Tegeder M. Function of pea amino acid permease AAP6 in nodule nitrogen metabolism and export, and plant nutrition. J Exp Bot. 2018; 69(21):5205–19. https://doi.org/10.1093/jxb/ery289
2. Tegeder M, Rentsch D. Uptake and partitioning of amino acids and peptides. Mol Plant. 2010;3(6):997–1011. https://doi.org/10.1093/mp/ssq047
3. Rahmoune B, Zerrouk IZ, Bouzaa S, Morsli A, Khelifi-Slaoui M, Ludwig-Müller J, Khelifi L. Amino acids profiling in Datura stramonium and study of their variations after inoculation with plant growth promoting Rhizobacteria. Biologia. 2019;74:1373–83. https://doi.org/10.2478/s11756-019-00287-y
4. Tegeder M, Weber APM. Metabolite transporters in control of primary plant metabolism. In: Plaxton WC, McManus MT, editors. Control of Primary Metabolism in Plants. 2006;85–120. https://doi.org/10.1002/9780470988640.ch4
5. Rentsch D, Schmidt S, Tegeder M. Transporters for uptake and allocation of organic nitrogen compounds in plants. FEBS Letters. 2007;581:2281–89. https://doi.org/10.1016/j.febslet.2007.04.013
6. Tegeder M. Transporters for amino acids in plant cells: some functions and many unknowns. Curr Opin Plant Biol. 2012;15:315–21. https://doi.org/10.1016/j.pbi.2012.02.001
7. Fischer WN, Kwart M, Hummel S, Frommer WB. Substrate specificity and expression profile of amino acid transporters (AAPs) in Arabidopsis. J Biol Chem. 1995;270(27):16315–20. https://doi.org/10.1074/jbc.270.27.16315
8. Fischer WN, Loo DD, Koch W, Ludewig U, Boorer KJ, Tegeder M, et al. Low and high affinity amino acid H+-cotransporters for cellular import of neutral and charged amino acids. Plant J. 2002;29(6):717–31. https://doi.org/10.1046/j.1365-313X.2002.01248.x
9. Svennerstam H, Ganeteg U, Bellini C, Näsholm T. Root uptake of cationic amino acids by Arabidopsis depends on functional expression of amino acid permease. New Phytol. 2008;180:620–30. https://doi.org/10.1111/j.1469-8137.2008.02589.x
10. Schwacke R, Schneider A, Van Der Graaff E, Fischer K, Catoni E, Desimone M, et al. Aramemnon, a Novel Database for Arabidopsis Integral Membrane Proteins. Plant Physiol. 2003;131(1):16–26. https://doi.org/10.1104/pp.011577
11. Jamtgard S, Näsholm T, Huss-Danell K. Characteristics of amino acid uptake in barley. Plant Soil. 2008;302:221–31. https://doi.org/10.1007/s11104-007-9473-4
12. Svennerstam H, Jämtgard S, Ahmad I, Huss-Danel K, Näsholm T, Ganeteg U. Transporters in Arabidopsis roots mediating uptake of amino acids at naturally occurring concentrations. New Phytol. 2011;191:459–67. https://doi.org/10.1111/j.1469-8137.2011.03699.x
13. Nithin, C, Patwa N, Thomas A, Bahadur RP, Basak J. Computational prediction of miRNAs and their targets in Phaseolus vulgaris using simple sequence repeat signatures. BMC Plant Biol. 2015;15:1–16. https://doi.org/10.1186/s12870-015-0516-3
14. Patwa N, Nithin C, Bahadur RP, Basak J. Identification and characterization of differentially expressed Phaseolus vulgaris miRNAs and their targets during mungbean yellow mosaic India virus infection reveals new insight into Phaseolus-MYMIV interaction. Genomics. 2019;111:1333–42. https://doi.org/10.1016/j.ygeno.2018.09.005
15. Tan Q, Grennan AK, Pélissier HC, Rentsch D, Tegeder M. Characterization and expression of French bean amino acid transporter PvAAP1. Plant Sci. 2008;174:348–56. https://doi.org/10.1016/j.plantsci.2007.12.008
16. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962;15:473–97.https://doi.org/10.1111/j.1399-054.1962.tb08052.x
17. Artimo P, Jonnalagedda M, Arnold K, Baratin D, Csardi G, de Castro E, et al. ExPASy: SIB bioinformatics resource portal. Nucleic Acids Res, 2012;40(W1):W597–W603. https://doi.org/10.1093/nar/gks400
18. Koressaar T, Lepamets M, Kaplinski L, Raime K, Andreson R, Reme M. Primer3_masker: integrating masking of template sequence with primer design software. Bioinformatics. 2018;34(11):1937–38. https://doi.org/10.1093/bioinformatics/bty036
19. Borges A, Tsai SM, Caldas DGG. Validation of reference genes for RT-qPCR normalization in common bean during biotic and abiotic stresses. Plant Cell Rep 2012;31:827–38. https://doi.org/10.1007/s00299-011-1204-x
20. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative CT method. Nat Protoc. 2008;3:1101–08. https://doi.org/10.1038/nprot.2008.73
21. Jones DL, Owen AG, Farrar JF. Simple method to enable the high resolution determination of total free amino acids in soil solutions and soil extracts. Soil Biol Biochem. 2002;34:1893–02. https://doi.org/10.1016/S0038-0717(02)00203-1
22. Hill PW, Quilliam RS, DeLuca TH, Farrar J, Farrell M, Roberts P, et al. Acquisition and Assimilation of Nitrogen as Peptide-Bound and D-Enantiomers of Amino Acids by Wheat. PLoS One. 2011;6: e19220. https://doi.org/10.1371/journal.pone.0019220
23. Raab TK, Lipson DA, Monson RK. Non-mycorrhizal uptake of amino acids by roots of the alpine sedge Kobresia myosuroides: implications for the alpine nitrogen cycle. Oecologia.1996;108:488–94. https://doi.org/10.1007/BF00333725
24. Öhlund J. Organic and inorganic nitrogen sources for conifer seedlings: abundance, uptake and growth. Doctoral thesis. Swedish University of Agricultural Sciences. PhD [Dissertation]. Umea: Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences; 2004. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-278
25. Wright DE. Amino acid uptake by plant roots. Arch Biochem Biophys. 1962;97:174–180. https://doi.org/10.1016/0003-9861(62)90061-9
26. Schmidt S, Stewart GR. Glycine metabolism by plant roots and its occurrence in Australian plant communities. Aust J Plant Physiol. 1999;26:253–64. https://doi.org/10.1071/PP98116
27. Owen AG, Jones DL. Competition for amino acids between wheat roots and rhizosphere micro-organisms and the role of amino acids in plant N acquisition. Soil Biol Biochem. 2001;33:651–57. https://doi.org/10.1016/S0038-0717(00)00209-1
28. Soldal T, Nissen P. Multiphasic uptake of amino acids by barley roots. Physiol Plant. 1978;43:181–88. https://doi.org/10.1111/j.1399-3054.1978.tb02561.x
29. Schobert C, Komor E. Amino-acid-uptake by Ricinus communis roots - characterization and physiological significance. Plant Cell Environ. 1987;10:493–500. https://doi.org/10.1111/j.1365-3040.1987.tb01827.x
30. Kielland K. Amino acid absorption by arctic plants: Implications for plant nutrition and nitrogen cycling. Ecology. 1994;75(8):2373–83. https://doi.org/10.2307/1940891
31. Nasholm T, Ekblad A, Nordin A, Giesler R, Hogberg M, Hogberg P. Boreal forest plants take up organic nitrogen. Nature. 1998;392:914–16. https://doi.org/10.1038/31921
32. Harrison J, Brugiere N, Phillipson B, Ferrario-Mery S, Becker T, Limami A, Hirel B. Manipulating the pathway of ammonia assimilation though genetic engineering and breeding: consequences to plant physiology and plant development. Plant Soil. 2000;221:81–93. https://doi.org/10.1023/A:1004715720043
33. Persson J, Nasholm T. Regulation of amino acid uptake in conifers by exogenous and endogenous nitrogen. Planta. 2002;215:639–44. https://doi.org/10.1007/s00425-002-0786-5
34. Gioseffi E, Neergaard Ade, Schjoerring JK. Interactions between uptake of amino acids and inorganic nitrogen in wheat plants. Biogeosciences. 2012;9:1509–18. https://doi.org/10.5194/bg-9-1509–2012
35. Lipson DA, Nasholm T. The unexpected versatility of plants: organic nitrogen use and availability in terrestrial ecosystems. Oecologia. 2001;128:305–16. https://doi.org/10.1007/s004420100693
36. Chakraborty N, Besra A, Basak J. Molecular Cloning of an Amino Acid Permease Gene and Structural Characterization of the Protein in Common Bean (Phaseolus vulgaris L.). Mol Biotechnol. 2020;62. https://doi.org/10.1007/s12033-020-00240-4
37. Garneau MG, Tan Q, Tegeder M. Function of pea amino acid permease AAP6 in nodule nitrogen metabolism and export, and plant nutrition. J Exp Bot. 2018; 69(21):5205–19. https://doi.org/10.1093/jxb/ery289
Downloads
Published
03-05-2020
How to Cite
1.
Patwa N, Chakraborty B, Basak J. Expression study of an Amino Acid Permease-like gene in Phaseolus vulgaris L. Plant Sci. Today [Internet]. 2020 May 3 [cited 2024 Nov. 4];7(2):251-6. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/712
Issue
Section
Research Articles
License
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
