The storage of biodiesel feedstock presents significant challenges for the biodiesel industry due to the deterioration of fatty acid constituents, which compromises both oil and biodiesel quality. Seed viability plays a critical role in preserving the quality of oil within biodiesel feedstock during storage. Recognizing the need for an affordable and efficient storage solution, this study aimed to develop a method for preserving the physiological and fuel characteristics of groundnut seeds (biodiesel feedstock) under tropical conditions. Groundnut seeds with a moisture content of 6.4% and an initial viability of 91% were stored in 3 different types of container: cloth bags, evacuated aluminum foil pouches and CO2-infused plastic containers for 7 months under ambient tropical conditions (34 ± 2 °C, 56 ± 4% RH). The study assessed the impact of storage conditions on seed viability, antioxidant enzyme activity and oil quality parameters, including free fatty acid (FFA), acid value (AV), peroxide value (PV), saponification value (SV), iodine value (IV) and cetane number (CV). Results indicated that CO2-infused plastic containers effectively maintained seed viability (72%), enhanced antioxidant enzyme activity and minimized oil deterioration. Furthermore, the feedstock stored in these containers complied with international biodiesel standards and remained suitable for alkali-based trans-esterification processes. This cost-effective storage method offers a viable solution for preserving biodiesel feedstock storage in tropical climates, thereby ensuring year-round biodiesel production and supply.