Chemical composition and antibacterial activities of the ethanol extracts from the leaves and tubers of Amorphophallus pusillus

Authors

  • Hong-Thien Van Institute of Biotechnology & Food-technology, Industrial University of Ho Chi Minh City, 12 Nguyen Van Bao Street, Go Vap District, Ho Chi Minh City, Vietnam
  • Hong Hai-My Nguyen Institute of Biotechnology & Food-technology, Industrial University of Ho Chi Minh City, 12 Nguyen Van Bao Street, Go Vap District, Ho Chi Minh City, Vietnam
  • Nguyen Tuong-An Huynh Office of Postgraduate Management, Industrial University of Ho Chi Minh City, 12 Nguyen Van Bao Street, Go Vap District, Ho Chi Minh City, Vietnam
  • Van-Son Le Binh Chau-Phuoc Buu Nature Reserve, Bung Rieng ward, Xuyen Moc District, Ba Ria-Vung Tau Province, Vietnam
  • Gia-Buu Tran Institute of Biotechnology & Food-technology, Industrial University of Ho Chi Minh City, 12 Nguyen Van Bao Street, Go Vap District, Ho Chi Minh City, Vietnam

DOI:

https://doi.org/10.14719/pst.2020.7.2.732

Keywords:

ethanol extract, LC/MS, antibacterial activity, Amorphophallus pusillus

Abstract

Amorphophallus pusillus is a rare species which is found only in Binh Chau-Phuoc Buu Nature Reserve. In this study, we determined 7 flavonoid compounds in tuber and leaf of A. pusillus, including of vitexin, orientin, vitexin 2?-O-glucoside, cyanidin 3-O-rutinoside, pelargonidin 3-O-glucoside, schaftoside, and peonidin 3-O-rutinoside via liquid chromatography-mass spectrometry (LC-MS). Furthermore, we have proved the antibacterial activities of ethanol extracts obtained from A. pusillus leaves and tubers in the first time. The data revealed that ethanol extracts could inhibit the growth of 6 tested microorganisms, such as Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Salmonella typhimurium, and Staphylococcus aureus. These data suggested the potential application of ethanol extracts isolated from this species as natural antimicrobial agents for treatment of infection caused by bacteria, especially in dermatologic and enteric infections.

Downloads

Download data is not yet available.

References

1. Pham HH. Araceae. In: Pham HH, editor. An Illustrated Flora of Vietnam. Ho Chi Minh City: Youth Publishing House; 2000. p. 356–63.

2. Boyce PC, Sookchaloem D, Hetterscheid WLA, Gusman G, Jacobsen N, Idei T, Nguyen VD. Araceae. The Flora of Thailand. 2002;11:130–86.

3. Nguyen VD. Flora of Vietnam-Araceae Juss. Vol. 16. Ha Noi (Vietnam): Publishing House for Sciense and Technology; 2017.

4. Tran VT, Ha VH, Nguyen MQ, Nguyen VD. Research component and distribution of species Amorphophallus spp. with tubers containing glucomannan in the Northern mountain provinces of Vietnam. Management of Forest Resources and Environment. 2017;5:118–25.

5. Singh A, Wadhwa NA. Review on multiple potential of Aroid: Amorphophallus paeoniifolius. International Journal of Pharmaceutical Sciences Review and Research. 2015;24:55–60.

6. Van HT, Tran NB, Trinh TT, Vo TNT, Le VS, Pham TV, et al.   Phytochemical composition and antibacterial activity of ethanol extract of Amorphophallus lanceolatus tuber (Araceae).  Banats Journal of Biotechnology [Internet]. 2019 [cited 2019 Dec 26];X(20):5–12. http://doi.org/10.7904/2068-4738-X(20)-5

7. Hetterscheid WLA, Claudel C. The end of Pseudodracontium N E. Br. Aroideana. 2012;35:40–46.

8. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology [Internet]. 1996 [cited 2019 Dec 26];45: 493–96. https://doi.org/10.1093/ajcp/45.4_ts.493

9. Iwashina T, Uehara A, Kitajima J, Yukawa T. Anthocyanins and other flavonoids from Amorphophallus titanum having largest inflorescence in plant kingdom, and other two species. Bull Natl Mus Nat Sci. Ser. 2015;41(1):33–44.

10. Lam KY, Ling APK, Koh RY, Wong YP, Say YH. A Review on Medicinal Properties of Orientin. Advances in Pharmacological Sciences [Internet]. 2016 [cited 2019 Dec 26]. https://doi.org/10.1155/2016/4104595

11. Aslam MS, Ahmad MS, Mamat AS. Pharmacological potential of vitexin. Indian Research Journal of Pharmacy and Science. 2015;2 (2):114–22.

12. Dong L, Fan Y, Shao X, Chen Z. Vitexin protects against myocardial ischemia/reperfusion injury in Langendorff perfused rat hearts by attenuating inflammatory response and apoptosis. Food Chem. Toxicol [Internet]. 2011 [cited 2019 Dec 26];49(12): 3211–16. https:// doi.org/10.1016/j.fct.2011.09.040

13. Lu Y, Yu T, Liu J, Gu L. Vitexin attenuates lipopolysaccharide-induced acute lung injury by controlling the Nrf2 pathway. PLoS ONE [Internet]. 2018 [cited 2019 Dec 26];13(4): e0196405.https://doi.org/10.1371/journal.pone.0196405

14. Amini AM, Muzs K, Jeremy PE, Yaqoob P. Pelargonidin-3-O-glucoside and its metabolites have modest anti-inflammatory effects in human whole blood cultures. Nutrition Research [Internet]. 2017 [cited 2019 Dec 26];46: 88–95. https://doi.org/10.1016/j.nutres.2017.09.006.

15. Feng R, Ni HM, Wang SY, Tourkova IR, Shurin MR, Harada H, Yin XM. Cyanidin-3-rutinoside, a natural polyphenol antioxidant, selectively kills leukemic cells by induction of oxidative stress. Journal of Biological Chemistry. 2007;282(18):13468–76.

16. Khan A, Rahman M, Islam MS. Antibacterial, antifungal and cytotoxic activities of amblyone isolated from Amorphophallus campanulatus. Indian J Pharmacol [Internet]. 2008 [cited 2019 Dec 26];40(1):41–44. https://doi.org/10.4103/0253-7613.40489

17. Muthukumaran P, Saraswathy N, Yuvapriya S, Balan R, Venkatesh BG, Palanikumar I. In vitro phytochemical screening and antibacterial activity of Amorphophallus paeonlifolius (Dennst.) Nicolson against some human pathogens. Journal of Chemical and Pharmaceutical Research. 2016;8:388–92.

18. Chidanand CS, Suryakant W, Farida I, Nikhil G, Kumar P. Antibacterial activity of Amorphophallus konkanensis and Amorphophallus bulbifer tuber. Asian Journal of Pharmaceutical and Clinical Research. 2015;8:98–102.

19. Cushnie TPT, Lamb AJ. Review Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents. 2005;26:343–56.

20. Basile A, Giordano S, Antonio JLS, Cobianchi RC. Antibacterial activity of pure flavonoids isolated from mosses. Phytochemistry [Internet]. 1999 [cited 2019 Dec 26];52(8): 1479–82. https://doi.org/10.1016/s0031-9422(99)00286-1

21. Ali H, Dixit S. In vitro antimicrobial activity of flavonoids of Ocimum sanctum with synergistic effect of their combined form. Asian Pacific Journal of Tropical Disease [Internet]. 2012 [cited 2019 Dec 26]; 2(1):396–98. https://doi.org/10.1016/S2222-1808(12)60189-3

22. Cisowska A, Wojnicz D, Hendrich AB. Anthocyanins as antimicrobial agents of natural plant origin. Nat Prod Commun. 2011;6(1):149–56.

23. PubChem Database. CID=442658. [Internet]. Bethesda MD: National Center for Biotechnology Information; 2005 [updated 2020 Feb 15, cited 2020 Feb 26]. Available from: https://pubchem.ncbi.nlm.nih.gov/compound/Schaftoside

Published

02-06-2020

How to Cite

1.
Van H-T, Nguyen HH-M, Huynh NT-A, Le V-S, Tran G-B. Chemical composition and antibacterial activities of the ethanol extracts from the leaves and tubers of Amorphophallus pusillus. Plant Sci. Today [Internet]. 2020 Jun. 2 [cited 2024 Nov. 4];7(2):296-301. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/732

Issue

Section

Research Articles

Most read articles by the same author(s)