Research on chemical constituents, anti-bacterial and anti-cancer effects of components isolated from Zingiber officinale Roscoe from Vietnam

Authors

DOI:

https://doi.org/10.14719/pst.2410

Keywords:

Zingiber officinale Roscoe, 6-shogaol, antimicrobial activities, anti-cancer activities, cytotoxic

Abstract

Ginger, a commonly used spice and medicinal herb, is an abundant source of bioactive compounds. However, the utilization of ginger in the pharmaceutical industry is still moderate and not commensurate with the potential of the Vietnamese horticulture industry, mainly due to a lack of information about the quality of input materials. In this study, we compared the volatile compounds of gingers collected from 13 provinces of Vietnam using GC/MS and GC-FID analysis to provide a basis for selecting and standardizing input materials. Furthermore, ginger essential oil from Ben Tre province of Vietnam exhibited significant antibacterial activity particularly in inhibiting Gram-positive bacteria, including S. aureus and S. epidermidis, with inhibition zones of 30.00 ± 1.41 and 24.67 ± 3.30 mm, respectively. However, no significant inhibition was observed against Gram-negative bacteria P. aeruginosa and E. coli. We also isolated 5 non-volatile compounds from ginger extract, namely 6-shogaol (1), quercetin (2), rutin (3), beta-sitosterol (4) and beta-sitosterol-3-O-beta-D-glucopyranoside (5). Among them, compounds 1–3 displayed cytotoxicity against Hep3B, SK-LU-1, MCF-7, SK-LU-1, SW480 and HepG2 tumour cell lines, with an IC50 values ranging between 62.7 ± 2.1 and 97.6 ± 1.1 µM, using Ellipticine as a positive control. Compounds 4 and 5 showed cytotoxicity against Hep3B and HepG2 tumor cells, with the IC50 values ranging between 21.5 ± 5.1 and 46.9 ± 3.7 µM but did not exhibit any significant cytotoxicity against SW480 and SK-LU-1 cells. Compound 4 also demonstrated middling cytotoxicity against the MCF7 cell line, with an IC50 value of 43.6 ± 5.1 µM. These findings suggest further applications of Vietnamese ginger for the treatment of infectious and cancer-related diseases.

Downloads

Download data is not yet available.

References

Loi DT, Vietnamese medicinal plants and remedies. Medical Publishing House. Hanoi, Vietnam; 2004.

Sharma S, Vijayvergia R, Singh T. Evaluation of antimicrobial efficacy of some medicinal plants. J Chem Pharm Res. 2010;2(1):121-24.

Chan EWC, Wong SK. Phytochemistry and pharmacology of ornamental gingers, Hedychium coronarium and Alpinia purpurata: A review. J Integr Med. 2015;13(6):368-79. https://doi.org/10.1016/S2095-4964(15)60208-4

Pereira MM, Haniadka R, Chacko PP, Palatty PL, Baliga MS. Zingiber officinale Roscoe (ginger) as an adjuvant in cancer treatment: A review. J BUON. 2011;16(3):414-24.

Peggy. Antimicrobial activity of medicinal plants. Afr J Biochem Res. 2006;12(3):379-99.

Islam K, Rowsni A, Khan MM, Kabir M. Antimicrobial activity of ginger (Zingiber officinale) extracts against food-borne pathogenic bacteria. Int J Environ Sci Technol. 2014;3(3):867-71.

Riaz H, Begum A, Raza SA, Khan ZM, Yousaf H, Tariq A. Antimicrobial property and phytochemical study of ginger found in local area of Punjab. Pak Inter Curr Pharma J. 2015;4(7):405-09. https://doi.org/10.3329/icpj.v4i7.23591

Habib SHM, Makpol S, Hamid NaA, Das S, Ngah WZW, Yusof YaM. Ginger extract (Zingiber officinale) has anti-cancer and anti-inflammatory effects on ethionine-induced hepatoma rats. Clinics. 2008;63(6):807-13. https://doi.org/10.1590/S1807-59322008000600017

Pham THT, Nguyen HL, Trinh VT. Analysis of the ginger value chain in Cao Bang province and recommendations for improvement. Centre for Agrarian Systems Research and Development (CASRAD). Ha Noi; 2019.

Hong TV, Uyen TXP, Gia BT. Chemical diversity of the Melaleuca cajuputi leaf oils from six locations in Southern Vietnam. Agriculturae Conspectus Scientificus. 2019;84(4):391-97.

Mounyr B, Moulay S, Saad KI. Methods for in vitro evaluating antimicrobial activity: A review. J Pharma Analy. 2016;6(2). https://doi.org/10.1016/j.jpha.2015.11.005

Ian AC. Cancer Cell Culture: Methods and Protocols, second edition. Methods in Molecular Biology. 2011;vol. 731. DOI 10.1007/978-1-61779-080-520.

Mosman T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J Immunol Method. 1983;65:55-63. https://doi.org/10.1016/0022-1759(83)90303-4

Yang L, Tu D, Zhao Z, Cui J. Cytotoxicity and apoptosis induced by mixed mycotoxins (T-2 and HT-2 toxin) on primary hepatocytes of broilers in vitro. Toxicon. 2017;129:1-10. https://doi.org/10.1016/j.toxicon.2017.01.001

Zhang J, Ma L, Wu ZF, Yu SL, Wang L, Ye WC, Zhang QW, Yin ZQ. Cytotoxic and apoptosis-inducing activity of C21 steroids from the roots of Cynanchum atratum. Steroid; 2017. https://doi.org/10.1016/j.steroids.2017.03.004

Krittika N, Natta L, Orapin K. Antibacterial effect of five zingiberaceae essential oils. Molecules. 2007;12:2047-60. https://doi.org/10.3390/12082047

Aaisha SAD, Fatema AAH, Khaloud MA, Hany MY, Wafa MAL, Syed NHA, Shah AK. Essential oil from the rhizomes of the Saudi and Chinese Zingiber officinale cultivars: Comparison of chemical composition, antibacterial and molecular docking studies. J King Saud Univ – Sci. 2020;32(8):3343-50. https://doi.org/10.1016/j.jksus.2020.09.020

Xin W, Yi S, Kiran T, Jinzhi H, Zhang JG, Fei H, Wei ZJ. Antibacterial activity and mechanism of ginger essential oil against Escherichia coli and Staphylococcus aureus. Molecules. 2020;25(17):395514. https://doi.org/10.3390/molecules25173955

Etna ICL, Mariana FH, Jenyffer MRM, Areli DRO, María TOM, Rocío SP, Tzasna HD. Antimicrobial activity of essential oil of Zingiber officinale Roscoe (Zingiberaceae). Am J Plant Sci. 2017;8(7):1511-24. https://doi.org/10.4236/ajps.2017.87104

Qingliang Q, Qizhen D. Preparation of the monomers of gingerols and 6-shogaol by flash high speed counter-current chromatography. 2011;1218(36):6187-90. https://doi.org/10.1016/j.chroma.2010.12.062

Bharathi S, Devasena T, Arivarasan A, Pachaiappan R. Extraction and isolation of flavonoid quercetin from the leaves of Trigonella foenum-graecum and their antioxidant activity. Int J Pharm Pharm Sci. 2016;8(6):120-24.

Taira J, Tsuchida E, Uehara M, Ohhama N, Ohmine W, Ogi T. The leaf extract of Mallotus japonicus and its major active constituent, rutin, suppressed on melanin production in murine B16F1 melanoma. Asian Pac J Trop Biomed. 2015;5(10):819-23. https://doi.org/10.1016/j.apjtb.2015.05.017

Rand AA, Ibrahim SA, Enas JK. Isolation and characterization of ?-sitosterol from Elaeagnus angustifolia cultivated in Iraq. Asian J Pharm Clin Res. 2018;11(11):442-46. https://doi.org/10.22159/ajpcr.2018.v11i11.29030

Nga VT, Trang NTH, Tuyet NTA, Phung NKP, Duong NTT, Thu NTH. Ethanol extract of male Carica papaya flowers demonstrated non-toxic against MCF-7, HEP-G2, HELA and NCI-H460 cancer cell lines. Vietnam J Chem. 2020;58(1):86-91. https://doi.org/10.1002/vjch.2019000142

Bawadood AS, Al-Abbasi FA, Anwar F, El-Halawany AM, Al-Abd AM. 6-shogaol suppresses the growth of breast cancer cells by inducing apoptosis and suppressing autophagy via targeting notch signaling pathway. Biomed and Pharma. 2020;128:110302. https://doi.org/10.1016/j.biopha.2020.110302.

Kim MO, Lee MH, Oi N, Kim SH, Bae KB, Huang Z, Kim DJ, Reddy K, Lee SY, Park SJ, Kim JY, Xie H, Kundu JK, Ryoo ZY, Bode AM, Surh YJ, Dong Z. (6)-shogaol inhibits growth and induces apoptosis of non-small cell lung cancer cells by directly regulating Akt1/2. Carcinog. 2014;35(3):683-91. https://doi.org/10.1093/carcin/bgt365.

Hu R, Zhou P, Peng YB, Xu X, Ma J, Liu Q, Zhang L, Wen XD, Qi LW, Gao N, Li P. 6-shogaol induces apoptosis in human hepatocellular carcinoma cells and exhibits anti-tumor activity in vivo through endoplasmic reticulum stress. PloS one. 2012;7(6):e39664. https://doi.org/10.1371/journal.pone.0039664.

Tamilselvam R, Pandian P, Venkatesan S, Sanjeev KS, Kandasamy R, Kasi PD. ?-sitosterol targets Trx/Trx1 reductase to induce apoptosis in A549 cells via ROS mediated mitochondrial dysregulation and p53 activation. Sci Rep. 2018;8:2071.https://doi.org/10.1038/s41598-018-20311-6.

Zhang XA, Zhang S, Yin Q, Zhang J. Quercetin induces human colon cancer cells apoptosis by inhibiting the nuclear factor-kappa B pathway. Pharma Magaz. 2015;11(42):404-09. https://doi.org/10.4103/0973-1296.153096

Osahon IR, Charles OA, Raghvendra RM, Juliana BA, Pragya M, Toluwase HF. Rediscovering medicinal activity and food significance of shogaol (4, 6, 8, 10, and 12): comprehensive review. Innovations in Food Technology: Current Perspectives and Future Goals. 2020; 125-45. https://link.springer.com/chapter/10.1007/978-981-15-6121-4_9

Anna KW, Marta HW, Magdalena I, Maciej G, Alina G, Dariusz G. Antiproliferative and antimetastatic action of quercetin on A549 non-small cell lung cancer cells through its effect on the cytoskeleton. Acta Histochem. 2017; 119(2): 99-112. https://pubmed.ncbi.nlm.nih.gov/27887793/

Abdur R, Muhammad I, Imtiaz AK, Mujeeb UR, Syed AG, Zaffar M, Mohammad SM. Anticancer potential of quercetin: A comprehensive review. Phytother. Res.. 2018; 32(11):2109-30. https://pubmed.ncbi.nlm.nih.gov/30039547/

Xiang AZ, Shuangxi Z, Qing Y, Jing Z. Quercetin induces human colon cancer cells apoptosis by inhibiting the nuclear factor-kappa B Pathway. Pharma Magaz. 2015;11(42): 404-09.

Amir I, Nasim M, Sepideh B, Maryam K, Simin S, Solmaz MD. Molecular mechanisms of anticancer effect of rutin. Phytother. Res.. 2021;35(5):2500-13.

Published

16-10-2023 — Updated on 01-01-2024

Versions

How to Cite

1.
Nguyen T-N, Nguyen K-AT, Le T-VN, Nguyen C-K, Nguyen N-TT, Kuo P-C, Tran G-B, Le N-A, Tran T-L, Nguyen N-T. Research on chemical constituents, anti-bacterial and anti-cancer effects of components isolated from Zingiber officinale Roscoe from Vietnam. Plant Sci. Today [Internet]. 2024 Jan. 1 [cited 2024 Nov. 21];11(1):156-65. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/2410

Issue

Section

Research Articles

Most read articles by the same author(s)