The study investigated the physiological and biochemical characteristics of peanut (Arachis hypogaea L.) varieties cultivated in the Andijan region of Uzbekistan. Analysis results revealed that during the flowering phase, the Chinese-275 variety had the highest chlorophyll "a" content in leaves (1.66 ± 0.12 mg/g). In comparison, the Tashkent-112 variety showed the highest chlorophyll "b" content (0.63 ± 0.15 mg/g). The Chinese-275 variety also exhibited the highest carotenoid content (0.65 ± 0.04 mg/g). In the pod formation phase, the Chinese-275 variety again demonstrated the highest chlorophyll "a" content in plant leaves (2.39 ± 0.06 mg/g), the Tashkent-112 variety had the highest chlorophyll "b" content (1.49 ± 0.52 mg/g) and the Chinese-275 variety showed the highest carotenoid content (0.98 ± 0.01 mg/g). In our experiments examining the protein and fat content of peanut (Arachis hypogaea L.) seeds, the Salomat variety exhibited the highest protein content (26.704 ± 0.303 %). The highest fat content was observed in the Mumtoz variety (0.467 ± 0.002 mg/g). These findings