Genetic diversity in threatened plant species Alnus nitida (Spach.) Endel

Authors

  • Muhammad Khalil Ullah Khan Department of Pomology, College of Horticulture, Agricultural University of Hebei, Baoding 071000, Hebei China
  • Noor Muhammad Department of Pomology, College of Horticulture, Agricultural University of Hebei, Baoding 071000, Hebei, China http://orcid.org/0000-0003-2788-1111
  • Nisar Uddin Department of Botany, Hazara University, Mansehra 21300, KP, Pakistan http://orcid.org/0000-0003-0944-7303
  • Niaz Ali Department of Botany, Hazara University, Mansehra 21300, KP, Pakistan http://orcid.org/0000-0001-7525-3916
  • Muhammad Umer College of Plant protection, Hebei Agricultural University, Baoding 071000, Hebei China
  • Shariat Ullah University of Malakand Chakdara 18800, KP, Pakistan

DOI:

https://doi.org/10.14719/pst.2020.7.3.759

Keywords:

Alnus nitida, conservation, genotypes, genetic variation, SDS-PAGE

Abstract

Alnus nitida (Spach) Endl. is an ethnobotanically important threatened plant species. The genetic diversity among the 50 different genotypes of Alnus nitida was carried out using sodium dodecyl sulfate poly acrylamide gel electrophoresis (SDS-PAGE) characterization. A considerable amount of genetic diversity (90%) was observed among the genotypes of A. nitida. The protein characterization was carried out on 12% gel electrophoresis. A total of 10 protein bands were detected in A. nitida genotypes. SDS-PAGE procedure is a useful method for the investigation of both genetic diversity and phylogenetic relationship. Especially, B-5 was monomorphic in A. nitida genotypes and was considered as species specific. All other bands/loci were polymorphic. These polymorphic bands displayed 12, 16, 72, 88, 2, 44, 84, 54 and 12 percent variation respectively. In the present examination, the high intra-specific diversity was observed representing SDS-PAGE is a powerful tool for determining the genetically diverse germplasms in A. nitida. The results obtained by this study could be helpful in the identification and selection of desired genotypes of Alnus nitida for conservation programmes in future. Today, there is still a need to assess genetic variation and protect genetic resources, especially of wild species for prospective benefits in plant conservation programmes.

Downloads

Download data is not yet available.

Author Biographies

Noor Muhammad, Department of Pomology, College of Horticulture, Agricultural University of Hebei, Baoding 071000, Hebei, China

Student, Hazara university of Mansehra, KP, Pakistan

Nisar Uddin, Department of Botany, Hazara University, Mansehra 21300, KP, Pakistan

PhD scholar, Hazara university Mansehra, KP, Pakistan

Niaz Ali, Department of Botany, Hazara University, Mansehra 21300, KP, Pakistan

Assistant Professor, Department of Botany, Hazara University Mansehra, KP, Pakistan

References

1. Muhammad N, Uddin N, Xuan Z, Umer M, Liu et al. Genetic diversity encourages conservation of threatened ethno medicinally important plant species in KozAbakhel District Swat, KP, Pakistan. Int J Botany Stud. 2019;4(4):151-61.

2. IUCN Species Survival Commission. IUCN Red List Categories, Version 3.1. Gland, Switzerland, Information Press, Oxford, UK, 2001, 21.

3. IUCN. IUCN Red List of Threatened species. www.iucnredlist.org cited on, 2008-2013

4. Chaudhri M, Qureshi RA. Pakistan Endangered Flora II: A Checklist of Rare and Seriously Threatened Taxa of Pakistan. Pak Syst. 1991;5(1-2):1-84.

5. Muhammad N, Uddin N, Ali N. Ethnomedicinal uses and inter specific diversity encourage conservation of Rhynchosia species growing in hilly areas of Swat. Agri Res & Tech: Open Access J. 2019;20(2): ARTOAJ.MS.ID.556124 .

6. Muhammad N, Wadood SF, Khan W, Ali N, Nisar M. Intra-species profiling of Cleome viscosa growing in Swat District (Pakistan). Biosyst. Divers. 2018; 26(1):52-55. http://doi.org/10.15421/011808

7. Noor M, Ali N, Nisar M, Abd Allah EF, Hashem A, et al. Genetic diversity within natural populations of the medicinal plant Rhynchosia minima (L.) DC. Appl EcolEnv Res. 2018;16(5):5633-51. http://dx.doi.org/10.15666/aeer/1605_56335651

8. Muhammad N, Uddin N, Khan MKU, Mengjun L, Xuan Z, Ali N, Liu Z. Ethnomedicinal and cultural uses of Ziziphus species in flora of Malakand Division KP, Pakistan. Singapore J Sci Res. 2020;10:1-7. https://scialert.net/abstract/?doi=sjsres.2020.1.7

9. Muhammad N, Uddin N, Khan MKK, Ali N. Inter Specific Genetic diversity within the ethnomedicinally important Ipomoea L. species through morphological and biochemical profiling. Int J Environ Sci Nat Res. 2019;19(3):556012. https://doi.org/10.19080/IJESNR.2019.19.556012

10. Muhammad N, Uddin N, Liu M, Ali N. Ethno medicine, antioxidant potential and inter-specific variations of Punica L. species growing in Swat District, KP, Pakistan. Int J Botany Stud. 2019;4(4):36-46. www.botanyjournals.com

11. Win KT, Zaw A, New KL, Thein MS, Yutaka H. Diversity of Myanmar cowpea accessions through seed storage polypeptides and its cross compatibility with the subgenus Ceratotropis. J Plant Breed Crop Sci. 2011;3(5):87-95. http://www.academicjournals.org/jpbcs

12. Ladizinsky G, Hymowitz, T. Seed protein electrophoresis in taxonomic and evolutionary studies. Theor Appl Genet. 1979;54(4):145–51. https://doi.org/10.1007/BF00263044

13. Potokina E, Vaughan DA, Eggi EE, Tomooka N. Population diversity of the Vicia sativa. (Fabaceae) in the flora of the former USSR deduced from RAPD and seed protein analysis. Genet Resour Crop Ev. 2000;47(2):171–83. https://doi.org/10.1023/A:1008756420011

14. Sammour RH. Using electrophoretic techniques in varietal identification, biosystematic analysis, phylogenetic relations and genetic resources management. JIAS. 1991;4:221-26.

15. Genetic Polymorphism within the wild population of Rhynchosia himalensis Benth. ex Baker. IJB. 2019;14(3):54-64. https://doi.org/10.12692/ijb/14.3.54-64

16. Hameed A, Shah TM, Atta BM, Iqbal N, Haq MA, Ali H. Comparative seed storage protein profiling of Kabuli chickpea genotypes. Pak J Bot. 2009; 41(2):703-10.

17. Ullah S, Muhammad N. Evaluation of informant consensus factor of medicinal plants used in Shamozai valley SWAT, KPK, Pakistan. International Journal of Medicinal Plant

Published

01-07-2020

How to Cite

1.
Khan MKU, Muhammad N, Uddin N, Ali N, Umer M, Ullah S. Genetic diversity in threatened plant species Alnus nitida (Spach.) Endel. Plant Sci. Today [Internet]. 2020 Jul. 1 [cited 2024 Nov. 4];7(3):314-8. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/759

Issue

Section

Research Articles