Skip to main navigation menu Skip to main content Skip to site footer
Plant Science Today (PST)

Research Articles

Vol. 12 No. 3 (2025)

Impact of physical and chemical mutagenesis on growth and flowering parameters in tuberose variety Arka Prajwal

DOI
https://doi.org/10.14719/pst.7606
Submitted
5 February 2025
Published
14-07-2025 — Updated on 24-07-2025
Versions

Abstract

The present study evaluated the effects of physical (gamma radiation) and chemical (ethyl methane sulphonate, EMS) mutagens on the growth and flowering parameters of Arka Prajwal in the M1V1 generation. Uniform-sized bulbs were subjected to gamma radiation (1.5 kR, 2.0 kR and 2.5 kR) and EMS treatments (0.3%, 0.4% and 0.5%) to induce variability. Growth parameters, including sprouting time, plant height, number of leaves, leaf dimensions and plant spread, exhibited significant variation across treatments. Gamma radiation delayed bulb sprouting (21.27–25.83 days) compared to control (12.53 days), while EMS treatments showed intermediate sprouting times (14.75–20.13 days). Plant height and leaf dimensions were highest in 0.4% EMS-treated plants, with notable increases in leaf width and plant spread. Flowering parameters demonstrated that EMS treatments accelerated spike emergence and floret opening compared to gamma irradiation. EMS-treated plants (0.3%) showed the earliest flowering, with reduced days to spike emergence (73.47 days) and first floret opening (90.38 days). Spike length and rachis length were slightly reduced in gamma-treated plants, while EMS-treated plants maintained longer spikes and rachis. The number of florets per spike was highest under EMS treatment (44.93 florets for 0.3%), accompanied by improved floret dimensions and weight. The study highlights that EMS enhanced growth and flowering performance in Arka Prajwal, while higher doses of gamma radiation adversely affected these parameters. This research provides insights into the potential of mutagenesis for improving floricultural traits in tuberose.

References

  1. 1. Sirohi U, Kumar M, Chauhan P, Kumar N, Prakash S, Chand P, et al. Genetic diversity in tuberose (Polianthes tuberosa L.) germplasm using Inter Simple Sequence Repeat (ISSR) markers. Int J Curr Microbiol Appl Sci. 2017;(6):1313–21. https://doi.org/10.20546/ijcmas.2017.605.142
  2. 2. Sreethramu GK, Bhat RN, Ranjanna KM. Studies on pollen viability, pollen germination and seed germination in tuberose hybrids and cultivars. South Indian Hortic. 2000;48(1):78–82. https://www.cabidigitallibrary.org/doi/full/10.5555/20013163047
  3. 3. Pooja K, Ranjan S, Manish K. Effect of physical and chemical mutagens on different cultivars of tuberose (Polianthes tuberosa Linn.) with particular reference to induction of genetic variability. Int J Agric Sci. 2016;8(56):3183–6.
  4. 4. Panse VG, Sukhatme PV. Statistical Methods for Agricultural Workers. New Delhi: Indian Council of Agricultural Research Publication. 1985;87–9.
  5. 5. Kayalvizhi K, Kannan M, Ganga M. Effects of gamma irradiation and chemical mutagens in tuberose Polianthes tuberosa L. Res Environ Life Sci. 2016;9(8):1030–2.
  6. 6. Datta SK. Induction and analysis of somatic mutations in garden chrysanthemum. Adv Hortic For. 1990;31:231–54.
  7. 7. Patil S. Induction of mutation in commercial varieties of gladiolus using physical mutagen, Co⁶⁰ gamma rays. Int J Adv Res Biol Sci. 2014;1(6):15–20.
  8. 8. Sisodia A, Singh A. Influence of gamma irradiation on morphological changes, postharvest life and mutagenesis in gladiolus. Int J Agric Environ Biotechnol. 2014;7(3):535. https://doi.org/http://dx.doi.org/10.5958/2230-732X.2014.01358.8
  9. 9. Singh PK, Sadhukhan R, Dudhane AS, Kumar V, Sarkar HS. Preliminary study on mutagenic effects of EMS on tuberose (Polianthes tuberosa L.). Environ Ecol. 2015;33(A):1386–90.
  10. 10. Dhivya M, Balakrishanan S, Thangaselvabai T. Sensitivity and mutagenic effects of ethyl methane sulphonate on the growth of Crossandra infundibuliformis L. Nees. Int J Agric Sci Res. 2015;5(3):199–203.

Downloads

Download data is not yet available.