Assessment of total phenolic content and antioxidant potentiality of selected Indian folk medicinal plants by spectrophotometric method
DOI:
https://doi.org/10.14719/pst.2020.7.3.765Keywords:
Medicinal plants, Leaf extract, Antioxidants, Polyphenols, In-vitro techniqueAbstract
Natural antioxidant capacity of five important folk medicinal plants measured in vitro. Total phenolic content (TPC), flavonoid content (FC) and free radical scavenging capacity of ethanolic, methanolic and aqueous leaf extracts of Lippia alba (LA), Annona squamosa (AS), Hyptis suaveolens (HS), Commiphora wightii (CW) and Milletia pinnata (MP) was assessed using spectrophotometric method. Folin ciocalteu and aluminium chloride method employed to optimise TPC and FC. Free radical scavenging potentiality of leaf extracts was assessed using Ferrous ion chelation (FIC), 2, 2'-diphenyl-1-picrylhydrazyl (DPPH•) scavenging, Hydroxyl (OH•) radical scavenging (HRS) and Superoxide (O- 2) radical scavenging (SRS) methods. Results revealed that the TPC (96.22±5.85 to 519.23±34.90 ?g GAE/gm dry weight) were found significant in aqueous extracts from all the plants except AS (p<0.05). For FC (?g QCE/gm dry weight), ethanol was found optimum for LA (463.94±6.49), CW (289.99±2.70) and MP (347.47±4.50) whereas, aqueous was found more appropriate for rest two plants were found significant instead of ethanol and methanol (all p<0.05). The lowest IC50 (?g/ml) were recorded from A. squamosa (27.72±8.95), H. suaveolens (27.78±0.88), C. wightii (27.18±0.16) and M. pinnata (27.30±0.03). All plants have reflected a high antioxidant capacity; however, the highest antioxidant activity was reported from ethanolic extract of H. suaveolens followed by L. alba, A. squamosa, C. wightii and M. pinnata. Hence, these studies show that all folk medicinal plants contain potential antioxidant bioactive compounds.
Downloads
References
2. Makari HK, Haraprasad N, Patil HS, Ravikumar S. In vitro antioxidant activity of the hexane and methanolic extracts of Cordia wallichii and Celastrus paniculatus. Internet J Aesthetic Anti-aging Med. 2008; 1:1-0. https://doi.org/10.5580/618
3. Dehariya R, Chandrakar J, Dubey S, Ojha K, Dixit AK. Scavenging and metal chelating potential of Carthamus tinctorius L. extracts. Current Botany. 2020 May 19:43-50. https://doi.org/10.25081/cb.2020.v11.6009
4. Halliwell B, Gutteridge JM. Free radicals in biology and medicine. Oxford University Press, USA; 2015. https://doi.org/10.1093/acprof:oso/9780198717478.001.0001
5. Kahl R, Kappus H. Toxicology of the synthetic antioxidants BHA and BHT in comparison with the natural antioxidant vitamin E. Zeitschrift fur Lebensmittel-untersuchung Und-forschung. 1993 Apr;196(4):329-38. https://doi.org/10.1007/BF01197931
6. Scalbert A, Manach C, Morand C, Rémésy C, Jiménez L. Dietary polyphenols and the prevention of diseases. Critical Reviews in Food Science and Nutrition. 2005 Jun 1;45(4):287-306. https://doi.org/10.1080/1040869059096
7. Walton NJ, Brown DE. Chemicals from plants: perspectives on plant secondary products, 1999. https://doi.org/10.1142/3203
8. Turkmen N, Sari F, Velioglu YS. Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin-Ciocalteu methods. Food Chemistry. 2006 Jan 1;99(4):835-41. https://doi.org/10.1016/j.foodchem.2005.08.034
9. Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010 Oct;15(10):7313-52. https://doi.org/10.3390/molecules15107313
10. Wang MY, West BJ, Jensen CJ, Nowicki D, Su C, Palu AK, Anderson G. Morinda citrifolia (Noni): a literature review and recent advances in Noni research. Acta Pharmacologica Sinica. 2002 Dec 1;23(12):1127-41
11. Wink M, editor. Biochemistry of plant secondary metabolism. CRC Press; 1999 Sep 21
12. Jones Jr JB, Case VW. Sampling, handling and analyzing plant tissue samples. Soil Testing and Plant Analysis. 1990 Jan 1;3:389-427. https://doi.org/10.2136/sssabookser3.3ed.c15
13. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture. 1965 Jan 1;16(3):144-58
14. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 2002 Jul 1;10(3)
15. Dinis TC, Madeira VM, Almeida LM. Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid per-oxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics. 1994;315(1):161-69. https://doi.org/10.1006/abbi.1994.1485
16. Klein SM, Cohen G, Cederbaum AI. Production of formaldehyde during metabolism of dimethyl sulfoxide by hydroxyl radical-generating systems. Biochemistry. 1981 Oct 1;20(21):6006-12. https://doi.org/10.1021/bi00524a013
17. Robak J, Gryglewski RJ. Flavonoids are scavengers of superoxide anions. Biochemical Pharmacology. 1988 Mar 1;37(5):837-41. https://doi.org/10.1016/0006-2952(88)90169-4
18. Harborne JB, Herbert H. Phytochemical dictionary: a Handbook of Bioactive Compounds from Plants. London: Taylor and Francis;1993
19. Marinova D, Ribarova F, Atanassova M. Total phenolics and total flavonoids in Bulgarian fruits and vegetables. J Univ Chem Tech Metall. 2005;40:255-60
20. Okpuzar J, Ogbunugafor H, Kareem GK, Igwo-Ezikpe MN. In vitro investigation of antioxidant phenolics compounds in extract of Senna alata. Res J Phytochem. 2009;3:68-76. https://doi.org/10.3923/rjphyto.2009.68.76
21. Sharma RK, Chatterji S, Rai DK, Mehta S, Rai PK, Singh RK, Watal G, Sharma B. Antioxidant activities and phenolic contents of the aqueous extracts of some Indian medicinal plants. J Med Plants Res. 2009 Nov 1;3(11):944-48
22. Mathew S, Abraham TE. In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies. Food and Chemical Toxicology. 2006;44(2):198-206. https://doi.org/10.1016/j.fct.2005.06.013
23. Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH. Comparison of ABTS, DPPH, FRAP and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis. 2006 Sep 1;19(6-7):669-75. https://doi.org/10.1016/j.jfca.2006.01.003
24. Joshi A, Prakash O, Pant AK, Kumar R, Negi MS. Chemical analysis and antioxidant activity of essential oils of two morpho types of Lippia alba (Mill.) N. E Br. ex Britton & P. Wilson (Verbenaceae). Journal of Essential Oil Bearing Plants. 2018 May 4;21(3):687-700. https://doi.org/10.1080/0972060X.2018.1486232
25. Shahat AA, Ibrahim AY, Alsaid MS. Antioxidant capacity and polyphenolic content of seven Saudi Arabian medicinal herbs traditionally used in Saudi Arabia. Indian Journal of Traditional Knowledge. 2015;14(1):28-35
26. Satoh K, Sakagami H. Effect of metal ions on radical intensity and cytotoxic activity of ascorbate. Anticancer Research. 1997;17(2A):1125-29
27. Yen GC, Duh PD, Tsai HL. Antioxidant and pro-oxidant properties of ascorbic acid and gallic acid. Food Chemistry. 2002 Nov 1;79(3):307-13. https://doi.org/10.1016/S0308-8146(02)00145-0
28. Dasgupta N, De B. Antioxidant activity of some leafy vegetables of India: A comparative study. Food Chemistry. 2007 Jan 1;101(2):471-74. https://doi.org/10.1016/j.foodchem.2006.02.003
29. Choudhary RK, Swarnkar PL. Antioxidant activity of phenolic and flavonoid compounds in some medicinal plants of India. Natural Product Research. 2011 Jul 1;25(11):1101-19. https://doi.org/10.1080/14786419.2010.498372
30. Luximon-Ramma A, Bahorun T, Soobrattee MA, Aruoma OI. Antioxidant activities of phenolic, proanthocyanidin and flavonoid components in extracts of Cassia fistula. Journal of Agricultural and Food Chemistry. 2002 Aug 28;50(18):5042-47. https://doi.org/10.1021/jf0201172
31. Silva MM, Santos MR, Caroço G, Rocha R, Justino G, Mira L. Structure-antioxidant activity relationships of flavonoids: a re-examination. Free Radical Research. 2002 Jan 1;36(11):1219-27. https://doi.org/10.1080/198-1071576021000016472
32. De Albuquerque UP, De Medeiros PM, De Almeida AL, Monteiro JM, Neto EM, de Melo JG, Dos Santos JP. Medicinal plants of the caatinga (semi-arid) vegetation of NE Brazil: a quantitative approach. Journal of Ethnopharmacology. 2007 Dec 3;114(3):325-54. https://doi.org/10.1016/j.jep.2007.08.017
33. Chandrakar J, Dubey S, Ojha K, Dehariya R, Dixit, AK. In vitro antioxidant activity, total phenolic contents and phytochemical evaluation from crude extracts of an endangered plant Cordia macleodii Hook. f. & Thomson. Journal of Biomedical and Pharmaceutical Research. 2019 June; 8(3):111-22. https://doi.org/10.32553/jbpr.v8i3.620
34. Di Stasi LC, Oliveira GP, Carvalhaes MA, Queiroz-Junior M, Tien OS, Kakinami SH, Reis MS. Medicinal plants popularly used in the Brazilian Tropical Atlantic Forest. Fitoterapia. 2002 Feb 1;73(1):69-91. https://doi.org/10.1016/S0367-326X(01)00362-8
35. Grassi P, Reyes TS, Sosa S, Tubaro A, Hofer O, Zitterl-Eglseer K. Anti-inflammatory activity of two diterpenes of Hyptis suaveolens from El Salvador. Zeitschriftfür Natur for schung C. 2006 Apr 1; 61(3-4):165-70. https://doi.org/10.1515/znc-2006-3-402
36. Yoganarasimhan SN. Medicinal Plants of India (Tamil Nadu), Vol II. Bangalore: Cyber Media. 2000;276
37. Sangwan S, Rao DV, Sharma RA. A review on Pongamia pinnata (L.) Pierre: A great versatile leguminous plant. Nature and Science. 2010; 8(11):130-39
Downloads
Published
How to Cite
Issue
Section
License
Copyright and Licence details of published articles
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Open Access Policy
Plant Science Today is an open access journal. There is no registration required to read any article. All published articles are distributed under the terms of the Creative Commons Attribution License (CC Attribution 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
