The radio-protective effects of n-Hexane extracts of Telfairia occidentalis Hook. f. and Cucumeropsis mannii Naud. seed oils on the liver of irradiated male Wistar rats
DOI:
https://doi.org/10.14719/pst.2020.7.3.805Keywords:
Radiation, Telfairia occidentalis, Cucumeropsis mannii, Seed-oil extracts, Antioxidants, LiverAbstract
The human population is predisposed to some considerable amounts of radiation especially ionizing radiation which may negatively impact their metabolic processes. Herbal extracts can mitigate these harmful effects. Therefore, this study aims to investigate the protective effect of n-Hexane extracts of Telfairia occidentalis and Cucumeropsis mannii seeds oils against radiation-mediated oxidative stress in Wistar rats. Sixty male rats were randomly distributed into six groups of six animals each and n-hexane extracts of T. occidentalis and C. mannii were administered at a dose of 2.4 or 4.8 mg/kg b. wt., orally for 7 days before irradiation and 10 days after irradiation, when they were sacrificed. Lipid peroxidation was measured, hepatic antioxidant status; SOD, CAT, GSH, Gpx and GST were estimated. The activities of liver enzymes: ALT, AST and ALP were measured and histological examination of sections of the liver was carried out. Radiation significantly increased MDA levels, SOD, GPx, AST, ALT and ALP activities but reduced body weights, total proteins, CAT, GSH and GST activities. Administration of the extracts significantly reduces the levels of MDA, SOD, GPx, ALT, AST and ALP activities while they increase the activities of CAT, GSH and GST at a dosage of 4.8 mg/kg. Histological examination showed increased levels of toxicity in radiated and groups administered 2.4 mg/kg extracts. From these findings, extracts of T. occidentalis and C. mannii at 4.8 mg/kg b. wt are effective herbal remedies in the prevention and amelioration of the consequences of oxidative stress due to exposure to ionizing radiation.
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38. Ogbuagu EO, Airaodion AI, Ogbuagu U, Airaodion EO. Prophylactic propensity of methanolic extract of Vernonia amygdalina leaves against acute ethanol-induced oxidative stress in wistar rats. Int J Biosci Biotechnol. 2019;11(7):37-46. https://www.researchgate.net/publication/334646848
39. Xie LH, Zhang XH, Hu XD, Min XY, Zhou QF, Zhang HQ.. Mechanisms of an increased level of serum iron in gamma-irradiated mice. Radiat Environ Biophys. 2016?55:81–88. https://doi.org/10.1007/s00411-015-0623-4
40. JosÉ MM, Cristina P, Ignacio NC. Antioxidant enzymes and human diseases. Clin Biochem. 1999;32(8):595-603. https://doi.org/10.1016/s0009-9120(99)00075-2
41. Cássia RP, Beny S. The effect of sixteen medicinal plants used in the Brazilian pharmacopoeia on the expression and activity of glutathione S-transferase in hepatocytes and leukemia cells. Pharm Biol. 2009?47(12):1192-97. https://doi.org/10.3109/13880200903029340
42. Ajani RS, Akinyemi AR. Telfairia occidentalis leaf and seed extract as possible preventive and therapeutic agents for induced benign prostatic hyperplasia. Eur J Med Plants. 2016; 12(1):1–11. https://doi.org/10.9734/EJMP/2016/22856
43. Owoade AO, Adetutu A, Airaodion AI, Ogundipe OO. Toxicological assessment of the methanolic leaf extract of Bridelia ferrugenia. The J Phytopharmacol. 2018?7(5):419-24. http://www.phytopharmajournal.com/Vol7_Issue5_03.pdf
44. Mansour HH. Protective role of carnitine ester against radiation-induced oxidative stress in rats. Pharmacol Res. 2006? 54: 165–71. http://dx.doi.org/10.1016/j.phrs.2006.04.003
45. Salem AM, Mohammaden TF, Ali MA, Mohamed EA, Hassan HF. Ellagic and ferulic acids alleviate gamma radiation and aluminium chloride-induced oxidative damage. Life Sci. 2016? 160:2–11. http://dx.doi.org/10.1016/j.lfs.2016.07.006
46. Longe OG, Farinu GO, Fetuga BL. Nutritious value of fluted pumpkin (Telfairia occidentalis). J Agric Food Chem. 1983? 31(5): 989-92. http://dx.doi.org/10.1021/jf00119a017
47. Okoye EI, Orakwue FC The chemical evaluation and anti-microbial screening of extracts from seeds and leaves of Telfaria occidentalis (Fluted Pumpkin). Chem Res J. 2019?4(3):98-104. https://www.researchgate.net/publication/336375204
2. El Kader MAA, Abulyazid I, Mohga SA, Hayat MS, Kamel WM. Evaluation of salicin isolated from Salix subserrata as a radioprotector against gamma irradiation induced ultrastructural and electrophoretic changes in spleen tissue in rats. UK J Pharm Biosci. 2015?3(2):46-59. http://dx.doi.org/10.20510/ukjpb/3/i2/89346
3. Lett JT. Damage to cellular DNA from particulate radiation, the efficacy of its processing and the radio-sensitivity of mammalian cells: Emphasis on DNA strand breaks and chromatin break. Radiat Environ Biophys. 1992;31:257-77. https://doi.org/10.1007/bf01210207
4. Daniniak N, Tann BJ. Utility of biological membranes as indicators for radiation exposure: alterations in membrane structure and function over time. Stem Cells. 1995;13:142-52. https://www.ncbi.nlm.nih.gov/pubmed/7488940
5. Maisin JR. Chemical radioprotection: past present and future prospects. Int J Radiat Biol. 1998?73:443-50. https://doi.org/10.1080/095530098142284
6. Coleman CN, Blackley WF, Fike JR, Mcvltie TJ, Meeting NF, Mitchell JB et al. Molecular and cellular biology of moderate-dose (1-10Gy) radiation and potential mechanisms of radiation protection: Report of a workshop at bethesda, Maryland, December, 17-18, 2001). Radiat Res. 2003?159:812-34. https://doi.org/10.1667/rr3021
7. Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect. 2001?109(1):69-75. https://doi.org/10.1289/ehp.01109s169
8. Airaodion AI, Ogbuagu EO, Ekenjoku JA, Ogbuagu U, Airaodion EO. Therapeutic effect of methanolic extract of Telfairia occidentalis leaves against acute ethanol-induced oxidative stress in wistar rats. Int J BioSci BioTechnol. 2019? 11(7):179-89. https://journals.eduindex.org/index.php/ijbsbt/article/view/6240
9. Achu MB, Fokou E, Tchiégang C, Fotso M, Tchouanguep FM. Nutritive values of some Cucurbitaceae oil seeds from different regions in Cameron. Afr J Biotechnol. 2005?4(10):1329-34. http://www.academicjournals.org/AJB
10. Besong SA, Ezekwe MO, Fosung CN, Senwo ZN. Evaluation of nutrient composition of African melon oilseed (Cucumeropsis mannii Naudin) for human nutrition. Int J Nutr Metab. 2011?3(8):103 -08. http://www.academicjournals.org/ijnam
11. Samaila RS, Chukwu O. Development of a motorized ‘egusi’ melon seeds oil expeller. J Agricult Engineer Technol. 2014?22(2):13-25. http://www.jaet.com.ng/index.php/Jaet/article/view/82/70
12. Onu PN. Effect of aqueous extract of Telfairia occidentalis leaf on the performance and haematological indices of starter broilers. Vet Sci. 2012;3(2):25–31. https://doi.org/10.5402/2012/726515
13. Eze BC, Ezejindu DN, Ogbodo EC, Ezeugwunne IP, Amah AK, Agada UN et al. The effects of aqueous leaf extract of Telfairia occidentalis (fluted pumpkin) on some hematological parameters in adult female wistar rats. J Prev Med Holist Health. 2019;5(2):106–09. https://doi.org/10.18231/j.jpmhh.2019.020
14. Kuku A, Etti UJ, Ibironke IS. Processing of fluted pumpkin seeds, Telfairia occidentalis (Hook. f.) as it affects growth performance and nutrient metabolism in rats. Afr J Food Agric Nutr Dev. 2014;14(5):1992–2014. https://www.researchgate.net/publication/285153151
15. Teugwa CM , Thaddée B, Bruno TT, Pascaline CM, Denis Z. Anti-hyperglycaemic globulins from selected Cucurbitaceae seeds used as antidiabetic medicinal plants in Africa. BMC Complement Altern Med. 2013?13:63-70. https://doi.org/10.1186/1472-6882-13-63
16. Oore-Oluwapo OD, Wahab AO, Gideon O. Effects of methanol extract of Telfairia occidentalis seed on serum lipid profile, biochemical and antioxidant activity in female wistar rats. Euro J Med Plants. 2016?15(2):1-8. https://doi.org/10.9734/EJMP/2016/26723
17. Tajudeen OJ, Adedayo OA, Ganiyu O, Aline AB. Phenolic extracts and amino acids content from Cucumeropsis mannii Naudin and Citrullus lanatus inhibit relevant enzymes of erectile dysfunction in rat's penile tissue. Biochem Biophys Rep. 2017?12:5–11. https://doi.org/10.1016/j.bbrep.2017.08.001
18. Krzyczkowska J, Koz?owska M. Effect of oils extracted from plant seeds on the growth and lipolytic activity of Yarrowia lipolytica yeast. J Am Oil Chem Soc. 2017?94:661–71. https://doi.org/10.1007/s11746-017-2975-1
19. Gornall AC, Bardawill EJ, David MM. Determination of serum proteins by means of Biuret reaction. J Biol Chem. 1949?177:364-65. http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.420.9605&rep=rep1&type=pdf
20. Varshney R, Kale RK. Effects of calmodulin antagonists on radiation- induced lipid peroxidation in microsomes. Int J Radiat Biol. 1990?58:733–43. https://doi.org/10.1080/09553009014552121
21. Sinha AK. Colorimetric assay of catalase. Anal Biochem. 1972? 47:389-94. https://doi.org/10.1016/0003-2697(72)90132-7
22. Misra HP, Fridovich I. The generation of superoxide radical auto-oxidation of hemoglobin. J Biol Chem. 1972;247:6960-62. https://www.jbc.org/content/247/21/6960.long
23. Hafeman DG, Sunde RA, Hoekste WG. Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. J Nutr. 1974?104:580–87. https://doi.org/10.1093/jn/104.5.580
24. Askelöf P, Guthenberg C, Jakobson I, Mannervik B. Purification and characterization of two glutathione S-aryltransferase activities from rat liver. Biochem J. 1975? 147:513–22. https://doi.org/10.1042/bj1470513
25. Beutler E, Duron O, Kelly BM. Improved method of determination of blood glutathione. J Lab Clin Med. 1963?61: 882-88. http://www.garfield.library.upenn.edu/classics1986/A1986A563500001.pdf
26. Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am J Clin Pathol. 1957;28:56-63. https://doi.org/10.1093/ajcp/28.1.56
27. Sankaranarayanan K. Estimation of the genetic risks of exposure to ionizing radiation in human: current status and emerging perspectives. J Radiat Res. 2006?47:57-66. https://doi.org/10.1269/jrr.47.b57
28. Dadheech G, Praveen S, Shiv G. Oxidative stress-induced response of some endogenous antioxidants in Schizophrenia. Ind J Clin Biochem. 2012?27(3):278–83. https://doi.org/10.1007/s12291-012-0193-z
29. Tetik S, Kiliç A, Aksoy H, Rizaner N, Ahmad S, Yardimci T. Oxidative stress causes plasma protein modification. Indian J Exp Biol. 2015;53(1):25-30. https://www.researchgate.net/publication/272187515
30. Lima CV, Tarcisio P, Ribeiro C. Radiation-induced changes in the electrophoretic profile of serum albumin. Braz Arch Biol Technol. 2016?59: e17160246. https://doi.org/10.1590/1678-4324-2016160246
31. Mishra KP. Cell membrane oxidative damage induced by ?-radiation and apoptotic sensitivity. J Environ Pathol Toxicol Oncol. 2004?23:1-72. https://doi.org/10.1615/jenvpathtoxoncol.v23.i1.60
32. Deepti D, Amit KD, Harsha L, Bhalla PJS, Deepak B. Protective effect of Terminalia chebula in modulating oxidative damages against gamma irradation induced lethality in rats. Int J Res Pharm Biomed Sci. 2012? 3(5):734-42.
33. Tiwari BK, Kanti BP, Abidi AB, Syed IR. Markers of oxidative stress during diabetes mellitus. J Biomarkers. 2013? Article ID 378790:1-8. https://doi.org/10.1155/2013/378790
34. Asmat U, Abad K, Ismail K. Diabetes mellitus and oxidative stress––A concise review. Saudi Pharm J. 2016?24:547–53. http://dx.doi.org/10.1016/j.jsps.2015.03.013
35. Eseyin OA, Udoh I, Ekpo A, Edoho EJ, Igboasoiyi AC. Biochemical effects of the seed extract of Telfairia occidentalis in rats. Int J Pharmacol. 2007?3:198-200. http://docsdrive.com/pdfs/ansinet/ijp/2007/198-200.pdf
36. Erejuwa OO. Oxidative stress in diabetes mellitus: Is there a role for hypoglycemic drugs and/or antioxidants. In: Lushchak V, editor. Oxidative Stress and Diseases. London: InTech Open; 2012. p. 217–46.
37. Jabir FA, Al Ali AS. Biochemical study and gene expression of Glutathione-S-Transferase (GST) in induced asthma in rat. Orient J Chem. 2015; 31(3): 1587-94. http://dx.doi.org/10.13005/ojc/310337
38. Ogbuagu EO, Airaodion AI, Ogbuagu U, Airaodion EO. Prophylactic propensity of methanolic extract of Vernonia amygdalina leaves against acute ethanol-induced oxidative stress in wistar rats. Int J Biosci Biotechnol. 2019;11(7):37-46. https://www.researchgate.net/publication/334646848
39. Xie LH, Zhang XH, Hu XD, Min XY, Zhou QF, Zhang HQ.. Mechanisms of an increased level of serum iron in gamma-irradiated mice. Radiat Environ Biophys. 2016?55:81–88. https://doi.org/10.1007/s00411-015-0623-4
40. JosÉ MM, Cristina P, Ignacio NC. Antioxidant enzymes and human diseases. Clin Biochem. 1999;32(8):595-603. https://doi.org/10.1016/s0009-9120(99)00075-2
41. Cássia RP, Beny S. The effect of sixteen medicinal plants used in the Brazilian pharmacopoeia on the expression and activity of glutathione S-transferase in hepatocytes and leukemia cells. Pharm Biol. 2009?47(12):1192-97. https://doi.org/10.3109/13880200903029340
42. Ajani RS, Akinyemi AR. Telfairia occidentalis leaf and seed extract as possible preventive and therapeutic agents for induced benign prostatic hyperplasia. Eur J Med Plants. 2016; 12(1):1–11. https://doi.org/10.9734/EJMP/2016/22856
43. Owoade AO, Adetutu A, Airaodion AI, Ogundipe OO. Toxicological assessment of the methanolic leaf extract of Bridelia ferrugenia. The J Phytopharmacol. 2018?7(5):419-24. http://www.phytopharmajournal.com/Vol7_Issue5_03.pdf
44. Mansour HH. Protective role of carnitine ester against radiation-induced oxidative stress in rats. Pharmacol Res. 2006? 54: 165–71. http://dx.doi.org/10.1016/j.phrs.2006.04.003
45. Salem AM, Mohammaden TF, Ali MA, Mohamed EA, Hassan HF. Ellagic and ferulic acids alleviate gamma radiation and aluminium chloride-induced oxidative damage. Life Sci. 2016? 160:2–11. http://dx.doi.org/10.1016/j.lfs.2016.07.006
46. Longe OG, Farinu GO, Fetuga BL. Nutritious value of fluted pumpkin (Telfairia occidentalis). J Agric Food Chem. 1983? 31(5): 989-92. http://dx.doi.org/10.1021/jf00119a017
47. Okoye EI, Orakwue FC The chemical evaluation and anti-microbial screening of extracts from seeds and leaves of Telfaria occidentalis (Fluted Pumpkin). Chem Res J. 2019?4(3):98-104. https://www.researchgate.net/publication/336375204
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19-07-2020
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NWOZO SO, UKEJE MO, IFIE JE. The radio-protective effects of n-Hexane extracts of Telfairia occidentalis Hook. f. and Cucumeropsis mannii Naud. seed oils on the liver of irradiated male Wistar rats. Plant Sci. Today [Internet]. 2020 Jul. 19 [cited 2024 Apr. 28];7(3):432-40. Available from: https://horizonepublishing.com/journals/index.php/PST/article/view/805
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