Skip to main navigation menu Skip to main content Skip to site footer

Research Articles

Vol. 12 No. sp3 (2025): Advances in Plant Health Improvement for Sustainable Agriculture

Tailoring cassava starch properties: A study on Heat-Moisture Treatment (HMT) and Annealing (ANN) for enhanced functional applications

DOI
https://doi.org/10.14719/pst.8250
Submitted
14 March 2025
Published
12-07-2025

Abstract

Native cassava starch was subjected to HMT and ANN under varying temperature and treatment duration conditions, as designed using a response surface methodology. The physicochemical properties of the modified starch were compared to those of native starch. The two treatments resulted in distinct alterations of the starch properties. HMT and ANN caused changes in solubility, swelling power, pasting properties, freeze-thaw stability, water and oil absorption capacities, water activity, colour and gelatinization characteristics. Solubility increased by 1.4 % and 3.81 % under HMT 25 % and HMT 30 % respectively, whereas ANN at 1: 3 starch-to-water ratio reduced solubility by 4.02 % compared to untreated starch. Swelling power decreased in the modified starch compared to the control sample. Viscosity studies revealed that peak viscosity decreased from 3812 cP in untreated sample to 3267 cP and 3150 cP after HMT - 25 % and HMT - 30 % respectively. In contrast, ANN increased the peak viscosity to 4014 cP. Freeze-thaw stability was narrowed for both HMT and ANN treated starches compared to the untreated starch. The treatments enhanced water absorption capacity but reduced oil absorption capacity (OAC). The clarity of the modified cassava starch pastes slightly decreased compared to the native starch. Moreover, the whiteness of the treated cassava starch powder was marginally lower than that of the untreated starch, though the differences were not statistically significant. These consequences disclosed that HMT and ANN techniques effectively modified the physicochemical properties of cassava starch.

References

  1. 1. Binang WB, Ittah MA, Obok EE, Effa EB, Bassey ES. Improving the yield and nutritional quality of cassava (Manihot esculenta Crantz) in the rainforest agro-ecological zone of southeast Nigeria through agronomic biofortification with micronutrients. J Plant Nutr. 2024;47(14):2232-41. https://doi.org/10.1080/01904167.2024.2338753
  2. 2. Kando K, Bitane B. Cultivation of cassava (Manihot Esculentus C.) with selected legumes for growth, yield and economic advantages as climate change mitigations. Int J Smart Agric. 2023;1(1):1-10. https://doi.org/10.54536/ijsa.v1i1.1682
  3. 3. Malik AI, Kongsil P, Nguyễn VA, Ou W, Sholihin, Srean P, et al. Cassava breeding and agronomy in Asia: 50 years of history and future directions. Breed Sci. 2020;70(2):145-66. https://doi.org/10.1270/jsbbs.18180
  4. 4. Putra LO, Suharti S, Sarwono KA, Sutikno S, Fitri A, Astuti WD, et al. The effects of heat-moisture treatment on resistant starch levels in cassava and on fermentation, methanogenesis and microbial populations in ruminants. Vet World. 2023;16(4):811-19. https://doi.org/10.14202/vetworld.2023.811-819
  5. 5. Jyothi AN, Sajeev MS, Sreekumar JN. Hydrothermal modifications of tropical tuber starches. 1. Effect of heat-moisture treatment on the physicochemical, rheological and gelatinization characteristics. Starch/Stärke. 2010;62(1):28-40. https://doi.org/10.1002/star.200900191
  6. 6. Jyothi AN, Sajeev MS, Sreekumar J. Hydrothermal modifications of tropical tuber starches-effect of ANN on the physicochemical, rheological and gelatinization characteristics. Starch/Stärke. 2011;63(9):536-49. https://doi.org/10.1002/star.201000137
  7. 7. Krishnakumar T, Sajeev M. Response surface optimization of bath type ultrasound-assisted extraction (UAE) of native starch from fresh cassava tubers. Adv Res. 2017;12(3):1-13. https://doi.org/10.9734/AIR/2017/37317
  8. 8. Dorantes-Fuertes M-G, López-Méndez MC, Martínez-Castellanos G, Meléndez-Armenta RÁ, Jiménez-Martínez H-E. Starch extraction methods in tubers and roots: a systematic review. Agronomy. 2024;14(4):865. https://doi.org/10.3390/agronomy14040865
  9. 9. Lee I, Kang T. Heat-moisture-treated rice starches with different amylose and moisture contents as stabilizers for nonfat yogurt. Food Chem. 2024; 436:137746. https://doi.org/10.1016/j.foodchem.2023.137746
  10. 10. Liu H, Guo X, Li W, Wang X, IV M, Peng Q, et al. Changes in physicochemical properties and in vitro digestibility of common buckwheat starch by heat-moisture treatment and annealing. Carbohydr Polym.. 2015; 132:237-44. https://doi.org/10.1016/j.carbpol.2015.06.071
  11. 11. Xie S, Li Z, Duan Q, Huang W, Huang W, Deng Y, et al. Reducing oil absorption in pea starch through two-step annealing with varying temperatures. Food Hydrocoll. 2024;150:109701. https://doi.org/10.1016/j.foodhyd.2023.109701
  12. 12. AOAC. Official method of analysis. 13th ed. Washington (DC): Association of Official Analytical Chemists. 1990;233-50.
  13. 13. AOAC International. Official method of analysis. 20th ed. Rockville (MD): Association of Official Analytical Chemists. 2016.
  14. 14. Moorthy S, Padmaja G. Starch content of cassava tubers. J Root Crops. 2002;28(1):30-7.
  15. 15. Krishnakumar T, Sajeev MS. Effect of ultrasound treatment on physicochemical and functional properties of cassava starch. Int J Curr Microbiol Appl Sci. 2018;7(10):3122-35. https://doi.org/10.20546/ijcmas.2018.710.362
  16. 16. Liu H, Lv M, Wang L, Li Y, Fan H, Wang M. Comparative study: how annealing and heat-moisture treatment affect the digestibility, textural and physicochemical properties of maize starch. Starch-Stärke. 2016;68(11-12):1158-68. https://doi.org/10.1002/star.201500268
  17. 17. Kaur P, Annapure US. Rheological and gelling properties of atmospheric pressure cold plasma treated finger millet (Eleusine coracana) starch. Food Res Int. 2024;187:114418. https://doi.org/10.1016/j.foodres.2024.114418
  18. 18. Sandhu KS, Singh N. Some properties of corn starches II: physicochemical, gelatinization, retrogradation, pasting and gel textural properties. Food Chem. 2007;101(4):1499-507. https://doi.org/10.1016/j.foodchem.2006.01.060
  19. 19. Cahyana Y, Wijaya E, Halimah TS, Marta H, Suryadi E, Kurniati D. The effect of different thermal modifications on slowly digestible starch and physicochemical properties of green banana flour (Musa acuminata colla). Food Chem. 2019;274:274-80. https://doi.org/10.1016/j.foodchem.2018.09.004
  20. 20. AOAC. Water activity. Washington (DC): Association of Official Analytical Chemists. 1980.
  21. 21. Agnes AC, Felix EC, Ugochukwu NT. Morphology, rheology and functional properties of starch from cassava, sweet potato and cocoyam. Asi J Biol. 2017;3(3):1-13. https://doi.org/10.9734/AJOB/2017/34587
  22. 22. Mhaske P, Wang Z, Farahnaky A, Kasapis S, Majzoobi M. Green and clean modification of cassava starch - effects on composition, structure, properties and digestibility. Crit Rev Food Sci Nutr. 2021;62(28):7801-26. https://doi.org/10.1080/10408398.2021.1919050
  23. 23. Falade KO, Ibanga-Bamijoko B, Ayetigbo OE. Comparing properties of starch and flour of yellow-flesh cassava cultivars and effects of modifications on properties of their starch. J Food Meas Charact.2019;13:2581-93. https://doi.org/10.1007/s11694-019-00178-5
  24. 24. da Rosa EZ, Dias ARG. Impact of heat-moisture treatment and annealing in starches: a review. Carbohydr Polym. 2011;83(2):317-28. https://doi.org/10.1016/j.carbpol.2010.08.064
  25. 25. Gunaratne A, Hoover R. Effect of heat-moisture treatment on the structure and physicochemical properties of tuber and root starches. Carbohydr Polym. 2002;49(4):425-37. https://doi.org/10.1016/S0144-8617(01)00354-X
  26. 26. Hormdok R, Noomhorm A. Hydrothermal treatments of rice starch for improvement of rice noodle quality. LWT Food Sci Technol. 2007;40(10):1723-31. https://doi.org/10.1016/j.lwt.2006.12.017
  27. 27. Olayinka OO, Adebowale KO, Olu-Owolabi BI. Effect of heat-moisture treatment on physicochemical properties of white sorghum starch. Food Hydrocoll.2008;22(2):225-30. https://doi.org/10.1016/j.foodhyd.2006.11.004
  28. 28. Chung H-J, Liu Q, Hoover R. Impact of annealing and heat-moisture treatment on rapidly digestible, slowly digestible and resistant starch levels in native and gelatinized corn, pea and lentil starches. Carbohydr Polym. 2009;75(3):436-47. https://doi.org/10.1016/j.carbpol.2008.08.006
  29. 29. Waduge R, Hoover R, Vasanthan T, Gao J, Li J. Effect of annealing on the structure and physicochemical properties of barley starches of varying amylose content. Food Res Int. 2006;39(1):59-77. https://doi.org/10.1016/j.foodres.2005.05.008
  30. 30. Lan H, Hoover R, Jayakody L, Liu Q, Donner E, Baga M, et al. Impact of annealing on the molecular structure and physicochemical properties of normal, waxy and high amylose bread wheat starches. Food Chem. 2008;111(3):663-75. https://doi.org/10.1016/j.foodchem.2008.04.055
  31. 31. Adebowale KO, Lawal OS. Effect of annealing and heat moisture conditioning on the physicochemical characteristics of Bambarra groundnut (Voandzeia subterranea) starch. Nahrung. 2002;46(5):311-6. https://doi.org/10.1002/1521-3803(20020901)46:5%3C311::AID-FOOD311%3E3.0.CO;2-Z
  32. 32. Abraham TE. Stabilization of paste viscosity of cassava starch by heat moisture treatment. Starch/Stärke. 1993;45(4):131-5. https://doi.org/10.1002/star.19930450404
  33. 33. Subroto E, Indiarto R, Marta H, Shalihah S. Effect of heat-moisture treatment on functional and pasting properties of potato (Solanum tuberosum L. var. Granola) starch. Food Res. 2019(5):469-76. https://doi.org/10.26656/fr.2017.3(5).110
  34. 34. Pertiwi SRR, Aminullah, Rajani RU, Novidahlia N. Effect of heat-moisture treatment on the physicochemical properties of native canistel starch. Food Sci Technol. 2022;42:e103921. https://doi.org/10.1590/fst.103921
  35. 35. Dutta D, Sit N. Comparison of properties of films prepared from potato starch modified by annealing and heat–moisture treatment. Starch. 2022;74(11-12):2200110. https://doi.org/10.1002/star.202200110
  36. 36. Hoover R, Manuel H. Effect of heat-moisture treatment on the structure and physicochemical properties of legume starches. Food Res Int. 1996;29(8):731-50. https://doi.org/10.1016/S0963-9969(97)86873-1
  37. 37. Devi R, Sit N. Effect of single and dual steps annealing in combination with hydroxypropylation on physicochemical, functional and rheological properties of barley starch. Int J Biol Macromol. 2019;129:1006-14. https://doi.org/10.1016/j.ijbiomac.2019.02.104
  38. 38. Adebowalea KO, Olu-Owolabi BI, Olayinka OO, Lawal OS. Effect of heat moisture treatment and annealing on physicochemical properties of red sorghum starch. Afr J Biotechnol.2005;4(9).
  39. 39. Lorenz K, Kulp K. Cereal- and root starch modification by heat-moisture treatment. I. physico-Chemical properties. Starch/Stärke. 1982;34(2):50-4. https://doi.org/10.1002/star.19820340205

Downloads

Download data is not yet available.