Review Articles
Vol. 13 No. sp1 (2026): Recent Advances in Agriculture
Microbial biofertilizers: A sustainable agricultural approach to augment crop resilience against biotic and abiotic stresses
Department of Life Sciences, Christ University, Bangalore 560 029, Karnataka, India
Department of Life Sciences, Christ University, Bangalore 560 029, Karnataka, India
Department of Life Sciences, Christ University, Bangalore 560 029, Karnataka, India
Abstract
Increasing plant growth and yield with the aid of plant growth-promoting bacteria is a widely accepted, eco-friendly and economic approach in modern agriculture. Their use as an alternative to widely used harmful chemical fertilizers and pesticides can improve overall soil health and fertility, thereby enhancing crop yield. The positive modulation of genes related to growth and development, fruit formation, stress tolerance and phytohormone production helps plants significantly during maturation. Furthermore, the enhanced production and expression of defence-related hormones aid in survival under various stress conditions, such as drought, pH fluctuations, salinity and water stress. In addition, the application of growth-promoting bacteria, mainly from species such as Azospirillum, Bacillus, Klebsiella, Enterobacter, Pseudomonas, Azotobacter, Burkholderia, Rhizobium, Alcaligenes, Arthrobacter and Serratia supports plant growth and development by improving soil porosity, pH and salinity conditions. This review briefly outlines the role of the plant growth-promoting bacteria as microbial biofertilizers that enhance crop resilience under both biotic and abiotic stress conditions. By highlighting recent advances in understanding the mechanisms of microbe-plant interactions under these stresses, it also provides unique insights into how microbial biofertilizers can be employed for sustainable agriculture. This has direct relevance for policymakers and farmers by reducing dependence on chemical fertilizers, which have various negative impacts, thereby promoting long-term environmental sustainability and improved agricultural practices.
References
- 1. Passioura JB. Soil conditions and plant growth. Plant Cell Environ. 2002;25(2):311–8. https://doi.org/10.1046/j.0016-8025.2001.00802.x
- 2. Mishra J, Singh R, Arora NK. Plant growth-promoting microbes: diverse roles in agriculture and environmental sustainability. In: Kumar V, Kumar M, Sharma S, Prasad R, editors. Probiotics plant health. Singapore: Springer; 2017. p. 71–111. https://doi.org/10.1007/978-981-10-3473-2_4
- 3. Ab Rahman SF, Singh E, Pieterse CM, Schenk PM. Emerging microbial biocontrol strategies for plant pathogens. Plant Sci. 2018;267:102–11. https://doi.org/10.1016/j.plantsci.2017.11.012
- 4. Singh HB. Management of plant pathogens with microorganisms. Proc Indian Natl Sci Acad. 2014;80(2):443–54. https://doi.org/10.16943/ptinsa/2014/v80i2/55120
- 5. Rani L, Thapa K, Kanojia N, Sharma N, Singh S, Grewal AS, et al. An extensive review on the consequences of chemical pesticides on human health and environment. J Clean Prod. 2021;283:124657. https://doi.org/10.1016/j.jclepro.2020.124657
- 6. Abdel Bagi AO, Ahmed AA, Elhindi M, Ali AM. Impact of pesticides and other chemicals on the environment. In: Workshop on Post Conflict National Plan for Environmental Management in Sudan; 2006. p. 18–20.
- 7. Pahalvi HN, Rafiya L, Rashid S, Nisar B, Kamili AN. Chemical fertilizers and their impact on soil health. In: Dar GH, Bhat RA, Mehmood MA, Hakeem KR, editors. Microbiota and biofertilizers. Vol. 2. Cham.: Springer; 2021. p. 1–20.
- 8. Baligar VC, Fageria NK, He ZL. Nutrient use efficiency in plants. Commun Soil Sci Plant Anal. 2001;32(7-8):921–50. https://doi.org/10.1081/CSS-100104098
- 9. Kumar S, Kumar S, Mohapatra T. Interaction between macro- and micro-nutrients in plants. Front Plant Sci. 2021;12:665583. https://doi.org/10.3389/fpls.2021.665583
- 10. Kudoyarova GR, Arkhipova TN, Melent’ev AI. Role of bacterial phytohormones in plant growth regulation. In: Maheshwari D, editor. Bacterial metabolites sustainable agroecosystem. Vol. 12. Cham.: Springer; 2015. p. 69–86. https://doi.org/10.1007/978-3-319-24654-3_4
- 11. Perrig D, Boiero ML, Masciarelli OA, Penna C, Ruiz OA, Cassán FD, et al. Plant-growth-promoting compounds produced by two agronomically important strains of Azospirillum brasilense, and implications for inoculant formulation. Appl Microbiol Biotechnol. 2007;75:1143–50. https://doi.org/10.1007/s00253-007-0909-9
- 12. Miljaković D, Marinković J, Balešević-Tubić S. Significance The significance of Bacillus spp. in disease suppression and growth promotion of field and vegetable crops. Microorganisms. 2020;8(7):1037. https://doi.org/10.3390/microorganisms8071037
- 13. Hashem A, Tabassum B, Abd_Allah EF. Bacillus subtilis: A plant-growth promoting rhizobacterium that also impacts biotic stress. Saudi J Biol Sci. 2019;26(6):1291–7. https://doi.org/10.1016/j.sjbs.2019.05.004
- 14. Mukherjee T, Banik A, Mukhopadhyay SK. Plant growth-promoting traits of a thermophilic strain of the Klebsiella group with its effect on rice plant growth. Curr Microbiol. 2020;77(10):2613–22. https://doi.org/10.1007/s00284-020-02032-0
- 15. Gopalakrishnan S, Sathya A, Vijayabharathi R, Varshney RK, Gowda CL, Krishnamurthy L. Plant growth promoting rhizobia. 3 Biotech. 2015;5:355–77. https://doi.org/10.1007/s13205-014-0241-x
- 16. Patil A, Kale A, Ajane G, Sheikh R, Patil S. Plant growth-promoting Rhizobium: mechanisms and biotechnological prospective. In: Hansen A, Choudhary D, Agrawal P, Varma A, editors. Rhizobium biology and biotechnology. Cham.: Springer; 2017. p. 105–34. https://doi.org/10.1007/978-3-319-64982-5_7
- 17. Gul N, Wani IA, Mir RA, Nowshehri JA, Aslam S, Gupta R, et al. Plant growth promoting microorganisms mediated abiotic stress tolerance in crop plants: a critical appraisal. Plant Growth Regul. 2023;100(1):7–24. https://doi.org/10.1007/s10725-022-00951-5
- 18. Wei X, Xie B, Wan C, Song R, Zhong W, Xin S, et al. Enhancing soil health and plant growth through microbial fertilizers: Mechanisms, benefits, and sustainable agricultural practices. Agronomy. 2024;14(3):609. https://doi.org/10.3390/agronomy14030609
- 19. Prashar P, Kapoor N, Sachdeva S. Rhizosphere: its structure, bacterial diversity and significance. Rev Environ Sci Biotechnol. 2014;13:63–77. https://doi.org/10.1007/s11157-013-9317-z
- 20. Santoyo G, Urtis-Flores CA, Loeza-Lara PD, Orozco-Mosqueda MD, Glick BR. Rhizosphere colonization determinants by plant growth-promoting rhizobacteria (PGPR). Biology. 2021;10(6):475. https://doi.org/10.3390/biology10060475
- 21. Hou J, Liu W, Wang B, Wang Q, Luo Y, Franks AE. PGPR enhanced phytoremediation of petroleum contaminated soil and rhizosphere microbial community response. Chemosphere. 2015;138:592–8. https://doi.org/10.1016/j.chemosphere.2015.07.025
- 22. Hassani MA, Durán P, Hacquard S. Microbial interactions within the plant holobiont. Microbiome. 2018;6:1–7. https://doi.org/10.1186/s40168-018-0445-0
- 23. Berg G. Plant–microbe interactions promoting plant growth and health: perspectives for controlled use of microorganisms in agriculture. Appl Microbiol Biotechnol. 2009;84(1):11–8. https://doi.org/10.1007/s00253-009-2092-7
- 24. Amaresan N, Murugesan S, Kumar K, Sankaranarayanan A, editors. Microbial mitigation of stress response of food legumes. Boca Raton: CRC Press; 2020. https://doi.org/10.1201/9781003028413
- 25. Hartmann A, Schmid M, Tuinen DV, Berg G. Plant-driven selection of microbes. Plant Soil. 2009;321:235–57. https://doi.org/10.1007/s11104-008-9814-y
- 26. Schirawski J, Perlin MH. Plant–microbe interaction 2017—the good, the bad and the diverse. Int J Mol Sci. 2018;19(5):1374. https://doi.org/10.3390/ijms19051374
- 27. Nadeem SM, Naveed M, Zahir ZA, Asghar HN. Plant–microbe interactions for sustainable agriculture: fundamentals and recent advances. In: Arora N, editor. Plant microbe symbiosis: fundamentals and advances. New Delhi: Springer; 2013. p. 51–103. https://doi.org/10.1007/978-81-322-1287-4_2
- 28. Niu B, Wang W, Yuan Z, Sederoff RR, Sederoff H, Chiang VL, et al. Microbial interactions within multiple-strain biological control agents impact soil-borne plant disease. Front Microbiol. 2020;11:585404. https://doi.org/10.3389/fmicb.2020.585404
- 29. Ortíz-Castro R, Contreras-Cornejo HA, Macías-Rodríguez L, López-Bucio J. The role of microbial signals in plant growth and development. Plant Signal Behav. 2009;4(8):701–12. https://doi.org/10.4161/psb.4.8.9047
- 30. Baker B, Zambryski P, Staskawicz B, Dinesh-Kumar SP. Signaling in plant-microbe interactions. Science. 1997;276(5313):726–33. https://doi.org/10.1126/science.276.5313.726
- 31. Köhl J, Kolnaar R, Ravensberg WJ. Mode of action of microbial biological control agents against plant diseases. Front Plant Sci. 2019;10:845. https://doi.org/10.3389/fpls.2019.00845
- 32. Balachandran S, Hurry VM, Kelley SE, Osmond CB, Robinson SA, Rohozinski J, et al. Concepts of plant biotic stress. Some insights into the stress physiology of virus-infected plants, from the perspective of photosynthesis. Physiol Plantarum. 1997;100(2):203–13. https://doi.org/10.1111/j.1399-3054.1997.tb04776.x
- 33. Amirsadeghi S, Robson CA, Vanlerberghe GC. The role of the mitochondrion in plant responses to biotic stress. Physiol Plantarum. 2007;129(1):253–66. https://doi.org/10.1111/j.1399-3054.2006.00775.x
- 34. Sergeant K, Renaut J. Plant biotic stress and proteomics. Curr Proteomics. 2010;7(4):275–97. https://doi.org/10.2174/157016410793611765
- 35. Bhar A, Chakraborty A, Roy A. Plant responses to biotic stress: Old memories matter. Plants. 2021;11(1):84. https://doi.org/10.3390/plants11010084
- 36. Zhao D, Wang H, Chen S, Yu D, Reiter RJ. Phytomelatonin: an emerging regulator of plant biotic stress resistance. Trends Plant Sci. 2021;26(1):70–82. https://doi.org/10.1016/j.tplants.2020.08.009
- 37. Ruiz-Ferrer V, Voinnet O. Roles of plant small RNAs in biotic stress responses. Annu Rev Plant Biol. 2009;60:485–510. https://doi.org/10.1146/annurev.arplant.043008.092111
- 38. Miller RN, Costa Alves GS, Van Sluys MA. Plant immunity: unravelling the complexity of plant responses to biotic stresses. Ann Bot. 2017;119(5):681–7. https://doi.org/10.1093/aob/mcw284
- 39. Bilgin DD, Zavala JA, Zhu J, Clough SJ, Ort DR, DeLucia EH. Biotic stress globally downregulates photosynthesis genes. Plant Cell Environ. 2010;33(10):1597–613. https://doi.org/10.1111/j.1365-3040.2010.02167.x
- 40. Zogli P, Libault M. Plant response to biotic stress: Is there a common epigenetic response during plant-pathogenic and symbiotic interactions? Plant Sci. 2017;263:89–93. https://doi.org/10.1016/j.plantsci.2017.07.008
- 41. Großkinsky DK, van der Graaff E, Roitsch T. Regulation of abiotic and biotic stress by plant hormones. In: Collinge DB, editor. Plant pathogen resistance biotechnology. Wiley; 2016. p. 131–54. https://doi.org/10.1002/9781118867716.ch7
- 42. Suzuki N, Rivero RM, Shulaev V, Blumwald E, Mittler R. Abiotic and biotic stress combinations. New Phytol. 2014;203(1):32–43. https://doi.org/10.1111/nph.12797
- 43. Wang L, Ning C, Pan T, Cai K. Role of silica nanoparticles in abiotic and biotic stress tolerance in plants: a review. Int J Mol Sci. 2022;23(4):1947. https://doi.org/10.3390/ijms23041947
- 44. Gupta A, Bano A, Rai S, Mishra R, Singh M, Sharma S, et al. Mechanistic insights of plant-microbe interaction towards drought and salinity stress in plants for enhancing the agriculture productivity. Plant Stress. 2022;4:100073. https://doi.org/10.1016/j.stress.2022.100073
- 45. Pettersson M, Bååth E. Temperature-dependent changes in the soil bacterial community in limed and unlimed soil. FEMS Microbiol Ecol. 2003;45(1):13–21. https://doi.org/10.1016/S0168-6496(03)00106-5
- 46. Khalilpour M, Mozafari V, Abbaszadeh-Dahaji P. Tolerance to salinity and drought stresses in pistachio (Pistacia vera L.) seedlings inoculated with indigenous stress-tolerant PGPR isolates. Sci Hortic. 2021;289:110440. https://doi.org/10.1016/j.scienta.2021.110440
- 47. Hardoim PR, van Overbeek LS, van Elsas JD. Properties of bacterial endophytes and their proposed role in plant growth. Trends Microbiol. 2008;16(10):463–71. https://doi.org/10.1016/j.tim.2008.07.008
- 48. Abd El-Ghany TM, Masrahi YS, Mohamed A, Abboud A, Alawlaqi MM, Elhussieny NI. Maize (Zea mays L.) growth and metabolic dynamics with plant growth promoting rhizobacteria under salt stresses. J Plant Pathol Microbiol. 2015;6(9):305.
- 49. Gusain YS, Singh US, Sharma AK. Bacterial mediated amelioration of drought stress in drought tolerant and susceptible cultivars of rice (Oryza sativa L.). Afr J Biotechnol. 2015;14(9):764–73. https://doi.org/10.5897/AJB2015.14405
- 50. Grover M, Madhubala R, Ali SZ, Yadav SK, Venkateswarlu B. Influence of Bacillus spp. strains on seedling growth and physiological parameters of sorghum under moisture stress conditions. J Basic Microbiol. 2014;54(9):951–61. https://doi.org/10.1002/jobm.201300250
- 51. Belimov AA, Dodd IC, Safronova VI, Shaposhnikov AI, Azarova TS, Makarova NM, et al. Rhizobacteria that produce auxins and contain 1-amino-cyclopropane-1-carboxylic acid deaminase decrease amino acid concentrations in the rhizosphere and improve growth and yield of well-watered and water-limited potato (Solanum tuberosum). Ann Appl Biol. 2015;167(1):11–25. https://doi.org/10.1111/aab.12203
- 52. Sharma M, Shilpa, Kaur M, Sharma AK, Sharma P. Influence of different organic manures, biofertilizers and inorganic nutrients on performance of pea (Pisum sativum L.) in North Western Himalayas. J Plant Nutr. 2023;46(4):600–17. https://doi.org/10.1080/01904167.2022.2071735
- 53. Kumar M, Mishra S, Dixit V, Kumar M, Agarwal L, Chauhan PS, et al. Synergistic effect of Pseudomonas putida and Bacillus amyloliquefaciens ameliorates drought stress in chickpea (Cicer arietinum L.). Plant Signal Behav. 2016;11(1):e1071004. https://doi.org/10.1080/15592324.2015.1071004
- 54. Tiwari S, Lata C, Chauhan PS, Nautiyal CS. Pseudomonas putida attunes morphophysiological, biochemical and molecular responses in Cicer arietinum L. during drought stress and recovery. Plant Physiol Biochem. 2016;99:108–17. https://doi.org/10.1016/j.plaphy.2015.11.001
- 55. Bresson J, Varoquaux F, Bontpart T, Touraine B, Vile D. The PGPR strain Phyllobacterium brassicacearum STM 196 induces a reproductive delay and physiological changes that result in improved drought tolerance in Arabidopsis. New Phytol. 2013;200(2):558–9. https://doi.org/10.1111/nph.12383
- 56. Yao L, Wu Z, Zheng Y, Kaleem I, Li C. Growth promotion and protection against salt stress by Pseudomonas putida Rs-198 on cotton. Eur J Soil Biol. 2010;46(1):49–54. https://doi.org/10.1016/j.ejsobi.2009.11.002
- 57. Zhou C, Ma Z, Zhu L, Xiao X, Xie Y, Zhu J, et al. Rhizobacterial strain Bacillus megaterium BOFC15 induces cellular polyamine changes that improve plant growth and drought resistance. Int J Mol Sci. 2016;17(6):976. https://doi.org/10.3390/ijms17060976
- 58. Zhang G, Sun Y, Sheng H, Li H, Liu X. Effects of the inoculations using bacteria producing ACC deaminase on ethylene metabolism and growth of wheat grown under different soil water contents. Plant Physiol Biochem. 2018;125:178–84. https://doi.org/10.1016/j.plaphy.2018.02.005
- 59. Vafadar F, Amooaghaie R, Otroshy M. Effects of plant-growth-promoting rhizobacteria and arbuscular mycorrhizal fungus on plant growth, stevioside, NPK, and chlorophyll content of Stevia rebaudiana. J Plant Interact. 2014;9(1):128–36. https://doi.org/10.1080/17429145.2013.779035
- 60. Kumar V, Khare T, Sharma M, Wani SH. ROS-induced signaling and gene expression in crops under salinity stress. In: Khan M, Khan N, editors. Reactive oxygen species and antioxidant systems in plants: role and regulation under abiotic stress. Singapore: Springer; 2007. https://doi.org/10.1007/978-981-10-5254-5_7
- 61. Glick BR, Cheng Z, Czarny J, Duan J. Promotion of plant growth by ACC deaminase-producing soil bacteria. In: Bakker PAHM, Raaijmakers JM, Bloemberg G, Höfte M, Lemanceau P, Cooke BM, editors. New perspectives and approaches in plant growth-promoting rhizobacteria research. Dordrecht: Springer. https://doi.org/10.1007/978-1-4020-6776-1_8
- 62. Serrano R, Gaxiola R. Microbial models and salt stress tolerance in plants. Crit Rev Plant Sci. 1994;13(2):121–38. https://doi.org/10.1080/07352689409701911
- 63. Numan M, Bashir S, Khan Y, Mumtaz R, Shinwari ZK, Khan AL, et al. Plant growth promoting bacteria as an alternative strategy for salt tolerance in plants: a review. Microbiol Res. 2018;209:21–32. https://doi.org/10.1016/j.micres.2018.02.003
- 64. Hong Z, Lakkineni K, Zhang Z, Verma DP. Removal of feedback inhibition of Δ1-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress. Plant Physiol. 2000;122(4):1129–36. https://doi.org/10.1104/pp.122.4.1129
- 65. Chakraborty K, Sairam RK, Bhattacharya RC. Differential expression of salt overly sensitive pathway genes determines salinity stress tolerance in Brassica genotypes. Plant Physiol Biochem. 2012;51:90–101. https://doi.org/10.1016/j.plaphy.2011.10.001
- 66. Gao M, Tao R, Miura K, Dandekar AM, Sugiura A. Transformation of Japanese persimmon (Diospyros kaki Thunb.) with apple cDNA encoding NADP-dependent sorbitol-6-phosphate dehydrogenase. Plant Sci. 2001;160(5):837–45. https://doi.org/10.1016/S0168-9452(00)00458-1
- 67. Abebe T, Guenzi AC, Martin B, Cushman JC. Tolerance of mannitol-accumulating transgenic wheat to water stress and salinity. Plant Physiol. 2003;131(4):1748–55. https://doi.org/10.1104/pp.102.003616
- 68. Inbaraj MP. Plant-microbe interactions in alleviating abiotic stress—a mini review. Front Agron. 2021;3:667903. https://doi.org/10.3389/fagro.2021.667903
- 69. Munir N, Hanif M, Abideen Z, Sohail M, El-Keblawy A, Radicetti E, et al. Mechanisms and strategies of plant microbiome interactions to mitigate abiotic stresses. Agronomy. 2022;12(9):2069. https://doi.org/10.3390/agronomy12092069
Downloads
Download data is not yet available.