Skip to main navigation menu Skip to main content Skip to site footer

Research Articles

Vol. 12 No. 3 (2025)

Enhancing rice seedling growth and vigour through liquid organic manure-based seed priming

DOI
https://doi.org/10.14719/pst.8976
Submitted
20 April 2025
Published
13-08-2025 — Updated on 27-08-2025
Versions

Abstract

Seed invigoration is a post-harvest treatment aimed at enhancing seed germination and seedling growth, with seed priming being one of the most important techniques in this category. A pot culture experiment was conducted in the net house of the Department of Agronomy, College of Agriculture, Vellayani, during September 2023. The objective of the study was to evaluate the effect of seed priming with liquid organic manures (LOMs) on the germination and seedling vigour of rice (Oryza sativa L.). The LOMs employed in the study included beejamrit, jeevamrit, panchagavya and vermiwash, each tested at four concentrations: 2.5 %, 3 %, 5 % and 6 %. Compared to control treatments (hydropriming and unprimed seeds), LOMs priming significantly enhanced germination parameters and seedling vigour. Among the treatments, vermiwash at 5 % concentration recorded higher values for germination index (125.3), germination speed (2.68) and mean daily germination (2.1). Panchagavya at 5 % concentration showed the highest germination rate index, whereas the coefficient of velocity of germination was greatest with vermiwash at 3 %. Beejamrit at 6 % resulted in the highest germination percentage, while seedling vigour index II was highest in vermiwash 6 %. Both treatments performed comparably to vermiwash 5 %. Although several treatments excelled in specific parameters, vermiwash 5 % consistently outperformed others across multiple germination metrics, indicating its strong potential as an effective seed priming agent for rice. Additionally, beejamrit and jeevamrit demonstrated specific advantages, suggesting their suitability for tailored applications based on crop-specific requirements or desired outcomes.

References

  1. 1. OECD-FAO Agricultural Outlook 2021–2030. Technical report, OECD and Food and Agriculture Organization of the United Nations; 2021. https://doi.org/10.1787/19428846-en
  2. 2. FAO. Crop Prospects and Food Situation—Quarterly Global Report No 2; 2021. https://doi.org/10.4060/cb5603en
  3. 3. Calpe C. Rice international commodity profile. Rome: Food and Agricultural Organization of the United Nations. 2006.
  4. 4. Pawar VA, Laware SL. Seed priming a critical review. International Journal of Scientific Research in Biological Sciences. 2018;5(5):94–101. https://doi.org/10.26438/ijsrbs/v5i5.94101
  5. 5. Farooq M, Tabassum R, Afzal I. Enhancing the performance of direct seeded fine rice by seed priming. Plant Production Science. 2006;9(4):446–56. https://doi.org/10.1626/pps.9.446
  6. 6. Bajehbaj AA. The effects of NaCl priming on salt tolerance in sunflower germination and seedling grown under salinity conditions. African Journal of Biotechnology. 2010;9(12). https://doi.org/10.5897/AJB10.1019
  7. 7. Paparella S, Araújo SS, Rossi G, Wijayasinghe MA, Carbonera D, Balestrazzi A. Seed priming: state of the art and new perspectives. Plant Cell Reports. 2015;34:1281–93. https://doi.org/10.1007/s00299 015 1784 y
  8. 8. Sumangala K, Patil MB. Panchagavya—an organic weapon against plant pathogens. Journal of Plant Disease Sciences. 2009;4(2):147–51.
  9. 9. Srimathi P, Mariappan N, Sundaramoorthy L, Paramathma M. Efficacy of panchagavya on seed invigoration of biofuel crops. Scientific Research and Essays. 2013;8(41):2031–7. https://doi.org/10.5897/SRE2013.5629
  10. 10. Ankad GM, Hiremath J, Patil RT, Pramod HJ, Hegde HV. Nutrient analysis of Kunapa jala and Panchagavya and their evaluation on germination of Ashwagandha and Kalamegha seeds: a comparative study. Journal of Ayurveda and Integrative Medicine. 2018;9(1):13–9. https://doi.org/10.1016/j.jaim.2017.01.011
  11. 11. Eswari JS, Dhagat S, Sen R. Biosurfactants, bioemulsifiers and biopolymers from thermophilic microorganisms. In: Thermophiles for Biotech Industry: A Bioprocess Technology Perspective. Singapore: Springer; 2019. p. 87–97. https://doi.org/10.1007/978-981-32-9919-1_5
  12. 12. Devi OR, Halder R, Pandey ST, Verma O, Chaturvedi P. Effect of seed priming with liquid organic on germination, seedling development and enzymatic activity of wheat (Triticum aestivum L.). Environment and Ecology. 2022;40(3 C):1720–5.
  13. 13. Sangeetha V, Thevanathan R. Effect of panchagavya on nitrate assimilation by experimental plants. American Journal of Science. 2010;6(2):76–82.
  14. 14. Kumar S, Thombare P, Kale P. Panchgavya: a boon in liquid fertilizer for organic farming. Agriculture and Food: E Newsletter. 2019;1(12):104–7.
  15. 15. Vyankatrao NP. Effect of beejamrita and other organic liquid treatments on seed germination and seedling growth of legume crops. International Multidisciplinary Research Journal. 2019;9(3):59–68.
  16. 16. Sreenivasa MN, Nagaraj MN, Bhat SN. Beejamruth: a source for beneficial bacteria. Karnataka Journal of Agricultural Sciences. 2010;17(3):72–7.
  17. 17. Devakumar N, Shubha S, Gowder SB, Rao GG. Microbial analytical studies of traditional organic preparations beejamrutha and jeevamrutha. Building Organic Bridges. 2014;2:639–42. https://doi.org/10.3220/REP_20_1_2014
  18. 18. Kumar RD, Rai PK, Bara BM, Raju GV. Pre sowing seed treatments with panchagavya, jeevamrutha and beejamrutha on growth, yield and yield attributing traits in chickpea (Cicer arietinum L.) variety RVG202. International Journal of Plant and Soil Science. 2022;34(22):1183–7. https://doi.org/10.9734/ijpss/2022/v34i2231483
  19. 19. Tiwari S, Chaurasia AK, Nithyananda N, BM B. Effect of organic priming on seed germination behaviour and vigour of chickpea (Cicer arietinum L.). Journal of Pharmacognosy and Phytochemistry. 2018;7(4):1064–7.
  20. 20. Sivasubramanian K, Ganeshkumar M. Influence of vermiwash on the biological productivity of marigold. Madras Agricultural Journal. 2004;91(4–6):221–5. https://doi.org/10.29321/MAJ.10.A00095
  21. 21. Rai N, Bansiwal K. Vermiwash: an excellent source of nutrition for plant growth. Electronic Journal of Environmental Sciences. 2008;1:19–21.
  22. 22. Manyuchi MM, Phiri A, Muredzi P, Chitambwe T. Comparison of vermicompost and vermiwash bio fertilizers from vermicomposting waste corn pulp. World Academy of Science, Engineering and Technology. 2013:365–8.
  23. 23. Awadhpersad VR, Ori L, Ansari AA. Production and effect of vermiwash and vermicompost on plant growth parameters of tomato (Lycopersicon esculentum Mill.) in Suriname. International Journal of Recycling of Organic Waste in Agriculture. 2021;10(4):397–413. https://doi.org/10.30486/IJROWA.2021.1911898.1148
  24. 24. Gudeta K, Julka JM, Kumar A, Bhagat A, Kumari A. Vermiwash: an agent of disease and pest control in soil, a review. Heliyon. 2021;7(3):e06434. https://doi.org/10.1016/j.heliyon.2021.e06434
  25. 25. Kerala Agricultural University. Package of Practices Recommendations (organic) Crops 2017. Thrissur: Kerala Agricultural University; 2017.
  26. 26. National Centre for Organic and Natural Farming. Module on one day farmers and demonstration on natural farming. Ghaziabad: National Centre for Organic and Natural Farming; 2022.
  27. 27. Jackson ML. Soil Chemical Analysis. New Delhi: Prentice Hall of India Pvt Ltd; 1973. p. 151–4.
  28. 28. Walkley A, Black IA. An examination of the Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Science. 1934;37(1):29–38. https://doi.org/10.1097/00010694-193401000-00003
  29. 29. Massoumi A, Cornfield AH. A rapid method for determining sulphate in water extracts of soils. Analyst. 1963;88(1045):321–2. https://doi.org/10.1039/an9638800321
  30. 30. Watt GW, Chrisp JD. Spectrophotometric method for determination of urea. Analytical Chemistry. 1954;26(3):452–3. https://doi.org/10.1021/ac60087a006
  31. 31. Casida Jr LE, Klein DA, Santoro T. Soil dehydrogenase activity. Soil Science. 1964;98(6):371–6. https://doi.org/10.1097/00010694-196412000-00004
  32. 32. Atlas RM. Handbook of microbiological media. CRC Press; 2004. https://doi.org/10.1201/9781420039726
  33. 33. Martin JP. Use of acid, rose bengal and streptomycin in the plate method for estimating soil fungi. Soil Science. 1950;69(3):215–32. https://doi.org/10.1097/00010694-195003000-00006
  34. 34. Cappuccino JG, Sherman N. Microbiology: A Laboratory Manual. San Francisco: Pearson/Benjamin Cummings; 2005.
  35. 35. International Seed Testing Association. International rules for seed testing. Seed Science and Technology; 1999.
  36. 36. Benech Arnold RL, Fenner M, Edwards PJ. Changes in germinability, ABA content and ABA embryonic sensitivity in developing seeds of Sorghum bicolor (L.) Moench induced by water stress during grain filling. New Phytologist. 1991;118(2):339–47. https://doi.org/10.1111/j.1469-8137.1991.tb00986.x
  37. 37. Esechie HA. Interaction of salinity and temperature on the germination of sorghum. Journal of Agronomy and Crop Science. 1994;172(3):194–9. https://doi.org/10.1111/j.1439-037X.1994.tb00166.x
  38. 38. Jones KW, Sanders D. The influence of soaking pepper seed in water or potassium salt solutions on germination at three temperatures. Journal of Seed Technology. 1987;11(1):97–102. https://doi.org/10.2307/23432941
  39. 39. Bartlett MS. Some examples of statistical methods of research in agriculture and applied biology. Journal of the Royal Statistical Society Series B. 1937;4(2):137–83. https://doi.org/10.2307/2983644
  40. 40. Ellis RH, Roberts EH. The quantification of ageing and survival in orthodox seeds. Seed Science and Technology. 1981;9(2):373–409.
  41. 41. Farooq MS, Basra SM, Saleem BA, Nafees M, Chishti SA. Enhancement of tomato seed germination and seedling vigour by osmopriming. Pakistan Journal of Agricultural Sciences. 2005;42:3–4.
  42. 42. Abdul Baki AA, Anderson JD. Vigour determination in soybean seed by multiple criteria. Crop Science. 1973;13(6):630–3. https://doi.org/10.2135/cropsci1973.0011183X001300060013x
  43. 43. Panse VG, Sukhatme PV. Statistical methods for agricultural workers. New Delhi: Indian Council of Agricultural Research; 1985.
  44. 44. Gopinath PP, Prasad R, Joseph B, VS A. grapesAgri1: collection of shiny apps for data analysis in agriculture. Journal of Open Source Software. 2021;6(63):3437. https://doi.org/10.21105/joss.03437
  45. 45. Anwar MP, Juraimi AS, Puteh A, Selamat A, Rahman MM, Samedani B. Seed priming influences weed competitiveness and productivity of aerobic rice. Acta Agriculturae Scandinavica Section B – Soil and Plant Science. 2012;62(6):499–509. https://doi.org/10.1080/09064710.2012.662244
  46. 46. Pavan BR, Mehta CM. Seed Invigouration: a review. The Pharma Innovation. 2021;10(8):595–600.
  47. 47. Prakash M, Ophelia AG, Narayanan GS, Anandan R, Baradhan G, Sureshkumar SM. Effect of organic seed pelleting on seedling quality, gas exchange, growth, yield and resultant seed quality parameters of black gram. Legume Research. 2020;43(2):221–8. https://doi.org/10.18805/LR 3965
  48. 48. Andoh H, Kobata T. Effect of seed hardening on seedling emergence and a amylase activity in the grains of wheat and rice sown in dry soil. Japanese Journal of Crop Science. 2002;71(2):220–5. https://doi.org/10.1626/jcs.71.220
  49. 49. Hussain S, Khan F, Hussain HA, Nie L. Physiological and biochemical mechanisms of seed priming induced chilling tolerance in rice cultivars. Frontiers in Plant Science. 2016;7:116. https://doi.org/10.3389/fpls.2016.00116
  50. 50. Patil PD, Chaurasia AK, Shukla PK, Dubey S, Kumari A. Impact of seed invigouration with panchagavya, beejamurtha on seed quality parameters in bitter gourd (Momordica charantia) under salinity conditions. Biology Forum. 2021;13(3):21–6.
  51. 51. Atish, Bharamaraj BA, Sivamurthy D. Effect of organic seed priming on seed quality of foxtail millet (Setaria italica L.). International Journal of Agricultural Sciences. 2023;15(6):12451–2.
  52. 52. Nayak H, Rai S, Mahto R, Rani P, Yadav S, Prasad SK, et al. Vermiwash: a potential tool for sustainable agriculture. Journal of Pharmacognosy and Phytochemistry. 2019;5(4):308–12.
  53. 53. Nahar K, Ashrafi R, Haque MA. Vermiwash: an effective nutritive blessing to crops. Bangladesh Journal of Nuclear Agriculture. 2024;38(1):21–40. https://doi.org/10.3329/bjnag.v38i1.76560
  54. 54. Anwar MP, Jahan R, Rahman MR, Islam AK, Uddin FM. Seed priming for increased seed germination and enhanced seedling vigour of winter rice. In: Proceedings of IOP Conference Series: Earth and Environmental Science; 2021. https://doi.org/10.1088/1755 1315/756/1/012047
  55. 55. Parihar P, Singh S, Singh R, Singh VP, Prasad SM. Effect of salinity stress on plants and its tolerance strategies: a review. Environmental Science and Pollution Research. 2015;22:4056–75. https://doi.org/10.1007/s11356 014 3739 1
  56. 56. Kharol M, Dhakar DL, Gurjar R, Choudhary R, Pandey S, Nagar G. Effect of liquid organic sources on growth, yield and quality of mung bean (Vigna radiata L.) under agroclimatic zone III A. International Journal of Research in Agronomy. 2024;7(12):369–72. https://doi.org/10.33545/2618060X.2024.v7.i12Sf.2180
  57. 57. Balasubramaniyan KK, Eeshwari I. Effect of panchagavya and bhejamrutha on seed germination, seedling growth and nutrient content in cucumber (Cucumis sativus L.). Indian Journal of Vegetable Research. 2019;45(1):355–61.
  58. 58. Kumar GD, Narayanan GS. Effect of organic seed invigouration on seed germination and vigour in rice cv CO 43. Journal of Environmental Biology Sciences. 2014;28(2):245–8.
  59. 59. Vijayan R, Krishnasamy V. Seed quality improvement of rice cv ADT 43 through organics. Environment and Ecology. 2015;33(4A):1749–53.

Downloads

Download data is not yet available.