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Plant Science Today (PST)

Research Articles

Vol. 12 No. sp4 (2025): Recent Advances in Agriculture by Young Minds - III

Growth, physiological and biochemical variations in okra infected by root-knot nematode

DOI
https://doi.org/10.14719/pst.9143
Submitted
27 April 2025
Published
22-10-2025

Abstract

Nematode infection causes significant change in the physiology of the plant. So, the study was conducted to investigate the impact of root knot nematode on okra. Six cultivars of okra were evaluated against root knot nematode; out of these, one was found to be resistant, two were moderately resistant and three were susceptible cultivars with the highest number of galls. In present study, the chlorophyll content of the susceptible cultivar was less than the moderately resistant and resistant cultivar. Maximum reduction was found in inoculated Utkal Gaurav 31.64 % as compared to Chota bana bhendi (18.44 %). In Chota bana bhendi, the chlorophyll b content was decreased from 5.4 to 4.6 mg/g of fresh leaf. Similarly, maximum reduction was seen in the case of susceptible cultivar Utkal Gaurav, i.e., 25.35 % and 22.34 % in Chota Bana Bhendi. The phenol content of inoculated roots of okra was increased by 15.40 %, 17.95 % and 34.91 % in cultivars Utkal Gaurav, Jhar bhendi and Chota bana bhendi respectively, over uninoculated plants. Whereas the proline content in roots of inoculated plants of okra was increased by 27.63 %, 21.51 % and 19.31 % in Utkal Gaurav, Jhar bhendi and Chota bana bhendi cultivars respectively. In all cultivars percentage macronutrient content significantly increased over the uninoculated plants, but the maximum increase recorded in Utkal gaurav shown Nitrogen (2.52 %), Phosphorus (1.6 %) and Potassium (3.43 %). The lowest concentration of Nitrogen (2.14 %) and Phosphorus (1.14 %) and Potassium (3.06 %) were observed in inoculated resistant cultivar. So, the identified resistant cultivar recommended for commercial cultivation.

References

  1. 1. Al-Wandawai H. Chemical composition of seeds of two okra cultivars Iraq. J Agric Food Chem. 1983. https://doi.org/10.1021/jf00120a051
  2. 2. Gowda MT, Rai AB, Singh B. Root knot nematode: a threat to vegetable production and its management (IIVR Technical Bulletin No. 76). Indian Institute of Vegetable Research, Varanasi. 2017.
  3. 3. Jones JT, Haegeman A, Danchin EGJ, Hari SG, Helder J, Jones MGK, et al. Top 10 plant-parasitic nematodes in molecular plant pathology. Mol Plant Pathol. 2013;14:946–61. https://doi.org/10.1111/mpp.12057
  4. 4. Silva MCL, Santos CDG, Silva GS. Espécies de Meloidogyne associada a vegetais em microrregiões do estado do Ceará. Rev Ciênc Agron. 2016;47:710–19.
  5. 5. Hunt DJ, Handoo ZA. Taxonomy, identification and principal species. In: Root Knot Nematodes. Wallingford, UK: CABI; 2009:55–97. https://doi.org/10.1079/9781845934927.0055
  6. 6. Hussain MA, Mukhtar T, Kayani MZ. Assessment of the damage caused by Meloidogyne incognita on okra (Abelmoschus esculentus). J Anim Plant Sci. 2011;21:857–61.
  7. 7. Hussain MA, Mukhtar T, Kayani MZ, Aslam MN, Haque MI. A survey of okra (Abelmoschus esculentus) in the Punjab province of Pakistan for the determination of prevalence, incidence and severity of root-knot disease caused by Meloidogyne spp. Pak J Bot. 2012;44(6):2071–5.
  8. 8. Mukhtar T, Arshad I, Kayani MZ, Hussain MA, Kayani MZ, Rahoo AM, et al. Estimation of damage to okra (Abelmoschus esculentus) by root knot disease incited by Meloidogyne incognita. Pak J Bot. 2013;45:1023–7.
  9. 9. Hussain MA, Mukhtar T, Kayani MZ. Responses to root-knot nematode (Meloidogyne incognita) infection in okra germplasm. Pak J Agric Sci. 2014;51(2):309–14.
  10. 10. Gowda MT, Rai AB, Singh B. Root knot nematode: a threat to vegetable production and its management. IIVR Tech Bull. 2007;76:32.
  11. 11. Sikora RA, Fernandez E. Nematode parasites of vegetables. In: Luc M, Sikora RA, Bridge J, editors. Plant Parasitic Nematodes in Subtropical and Tropical Agriculture. London, UK: CABI; 2005:319–92. https://doi.org/10.1079/9780851997278.0319
  12. 12. Devran Z, Burcu B, Ays T, Fatmana D. Comparison of PCR-based molecular markers for identification of Mi gene. Acta Agric Scand B Soil Plant Sci. 2013. https://doi.org/10.1080/09064710.2013.771700
  13. 13. Williamson VM, Robert PA. Mechanisms and genetics of resistance. In: Perry RN, Moens M, Starr JL, editors. Root Knot Nematodes. CABI Publishing; 2009:301–19. https://doi.org/10.1079/9781845934927.0301
  14. 14. Eisenback JD, Hirschmann H, Sasser JN, Triantaphyllou AC. A guide to the four most common species of root knot nematodes, Meloidogyne spp. with a pictorial key. Raleigh, North Carolina, USA: North Carolina State University Graphics and USAID; 1981:48.
  15. 15. Taylor AL, Sasse JN. Biology, identification and control of root-knot nematode Meloidogyne incognita. Raleigh, NC, USA: North Carolina State University and United States Agency for International Development; 1978:3.
  16. 16. Arnon DI. Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol. 1949;24:1–5. https://doi.org/10.1104/pp.24.1.1
  17. 17. Bray HG, Thrope WV. Analysis of phenolic compounds of interest in metabolism. Methods Biochem Anal. 1954;1:27–52. https://doi.org/10.1002/9780470110171.ch2
  18. 18. Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water stress studies. Plant Soil. 1973;39:205. https://doi.org/10.1007/BF00018060
  19. 19. Mahadevan A, Sridhar R. Methods in physiological plant pathology. Madras: Sivakami Publications; 1986.
  20. 20. Jackson ML. Soil Chemical Analysis. New Delhi: Prentice Hall of India Pvt. Ltd.; 1973:498.
  21. 21. Trudgill. Resistance to and tolerance of plant parasitic nematodes in plants. Annu Rev Phytopathol. 1991;29:167–92. https://doi.org/10.1146/annurev.py.29.090191.001123
  22. 22. Ye DY, Qi YH, Cao SF, Wei BQ, Zhang HS. Histopathology combined with transcriptome analyses reveals the mechanism of resistance to Meloidogyne incognita in Cucumis metuliferus. J Plant Physiol. 2017;212:115–24. https://doi.org/10.1016/j.jplph.2017.02.002
  23. 23. Gillet FX, Bournaud C, de Souza Júnior JDAD, Grossi-de-Sa MF. Plant-parasitic nematodes: towards understanding molecular players in stress responses. Ann Bot. 2017;119:775–89. https://doi.org/10.1093/aob/mcw260
  24. 24. Labudda M. Ascorbate-glutathione pathway as an important player in redox regulation in nematode-infested plants: what we have learned so far. Physiol Mol Plant Pathol. 2018;103:47–53. https://doi.org/10.1016/j.pmpp.2018.04.007
  25. 25. Atia MAM, Abdeldaym EA, Abdelsattar M, Ibrahim DSS, Saleh I. Piriformospora indica promotes cucumber tolerance against root-knot nematode by modulating photosynthesis and innate responsive genes. Saudi J Biol Sci. 2020;27:279–87. https://doi.org/10.1016/j.sjbs.2019.09.007
  26. 26. Labudda M, Tokarz K, Tokarz B, Muszyska E, Gietler M. Reactive oxygen species metabolism and photosynthetic performance in leaves of Hordeum vulgare plants co-infested with Heterodera filipjevi and Aceria tosichella. Plant Cell Rep. 2020;39:1719–41. https://doi.org/10.1007/s00299-020-02600-5
  27. 27. Agarwal ML, Goel AK, Kumar S, Tayal MS. Biochemical changes in okra infected with root-knot nematode, Meloidogyne incognita, some hydrolysing and oxidative enzymes and related chemical metabolites. Indian J Nematol. 1985;15(2):255–7.
  28. 28. Ahmed N, Abbasi MW, Shaukat SS, Zaki MJ. Physiological changes in leaves of mungbean plants infected with Meloidogyne javanica. Phytopathol Mediterr. 2009;48(2):262–8.
  29. 29. Danish M, Seikh H, Robab MI. Study on morphological and biochemical characteristics of babchi (Psoralea corylifolia) infected with the root-knot nematode, Meloidogyne incognita. J Agric Sci Technol. 2018;20(3):633–45.
  30. 30. Nayak DK. Effects of nematode infection on contents of phenolic substances as influenced by root-knot nematode, Meloidogyne incognita in susceptible and resistant brinjal cultivars. Agric Sci Dig Res J. 2015;35(2):163–4. https://doi.org/10.5958/0976-0547.2015.00032.4
  31. 31. Patel VS, Pitambara, Shukla YM. Biochemical characterization of root knot nematode (Meloidogyne incognita) infected tomato cultivar (Solanum lycopersicum L.). J Pharmacogn Phytochem. 2018;7(5):1621–9.
  32. 32. Mahapatra M, Nayak DK. Variation in nutrient status of susceptible and resistant bitter gourd varieties infected by root knot nematode, Meloidogyne incognita. J Entomol Zool Stud. 2019;8(1):1639–46.

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