Applying urea is becoming more and more rapidly rising worldwide; nonetheless, the present study aimed to investigate the influence of using nitrogen fertilizer in the soil application in conjunction with foliar spraying of urea and nano-urea, the study was carried out to explore the effects of nitrogen (N) fertilizer on chlorophyll content in maize, during the rabi season of 2024, aiming to compare chlorophyll concentration at different growth stages and its influence on grain yield. The experiment was conducted using a randomized block design with seven treatments and three replications. The research was conducted in the SRM College of Agricultural Science farm, Baburayanpettai, Chengalpattu, Tamil Nadu, India. Results showed that the interaction of N with treatment T4 recorded the highest total chlorophyll content, leaf area index, stover yield and grain yield. This indicates that structuring N fertilizer application using this combination can enhance uptake efficiency, minimize leaching losses and improve crop performance.