To assess the shelf life of white button mushrooms (WBM), three different packaging films were used: a control film composed of PLA-PBAT, a glycerol-plasticized chitosan film and a chitosan film enhanced with glycerol and reinforced with CNC derived from spent lemongrass (chitosan-CNC film). WBM packed in these three types of packaging films were evaluated by recording the parameters like weight loss (WL), poly phenol oxidase (PPO) activity, decay loss (DL), browning index (BI), visual appearance (VA), odour (OD) and overall acceptability (OA) for a period of 12 days with an interval of 3 days. All the parameters were significantly affected (p < 0.05) with the type of packaging film and storage period. The WBM remained acceptable up to the 3rd day of storage in chitosan film packages, 6th day of storage in chitosan-CNC film packages and 9th day of storage in control film packages under refrigerated condition of 4 °C without any appreciable loss of overall quality parameters.