Wheat (Triticum spp.) was known to be mostly grown cereal crops globally and consumed as staple food by 35 % population of the world. The average productivity may decrease throughout the world due to outbreak of Fusarium graminearum which cause Fusarium head blight disease in wheat crop. This research was conducted to find potential of these 11 endophytic fungus and bacteria isolated from wheat crop and 2 plant extracts which were evaluated as biological control agents and leaf leachates against Fusarium graminaearum as an alternative eco-friendly approach for chemical pesticides due to resistance occur in pathogens by overdose and some harmful chemical residues left by them in soil. The data revealed that maximum mycelial inhibition of test pathogen was showed by Aspergillus niger (93.32 %) followed by Curvularia lunata (90.31 %) and Bacillus subtilis (89.41 %). The data revealed that maximum mycelial inhibition of test pathogen was carried out by methanol + Ageratum conyzoides in 6 % and 4 % conc. (98.29 %) and (98.06 %) followed by ethanol + Parthenium hysterophorus in 6 % conc. (97.78 %).