Light quality plays a pivotal role in plant development and the biosynthesis of secondary metabolite. Jasminum sambac is a medicinally valuable plant known for its diverse phytochemicals with pharmacological significance. This study investigated the impact of red (600-700 nm) and far-red (700-750 nm) light treatments on the phytochemical profile of J. sambac CO. 1 using gas chromatography-mass spectrometry (GC-MS). Plants subjected to 80 % red and 20 % far-red light exhibited significant metabolic shifts compared to untreated controls, with enhanced accumulation of bioactive compounds such as 12-oleanen-3-yl acetate, cis-vaccenic acid and 9,19-cyclolanost-24-en-3-ol, each
associated with anti-inflammatory, antimicrobial, antioxidant and anticancer properties. The findings highlight the role of light in modulating secondary metabolites, offering insights into optimized cultivation strategies for pharmaceutical and aromatic applications. This study underscores the potential of spectral light manipulation to enhance medicinal compound biosynthesis in J. sambac CO. 1.