Agroforestry systems, which integrate trees into agricultural landscapes, offer significant potential for carbon sequestration. This study evaluates carbon sequestration under prevalent agroforestry systems in Sohawal and Rudauli blocks of Ayodhya district, Uttar Pradesh. Carbon storage was assessed in tree biomass, soil and deadwood using standardised protocols across various agroforestry practices. Results indicated that Agri-Horticulture (AH) (Mango + Mustard) had the highest 107 cm average tree girth at breast height (GBH) in Sohawal, while AH (Mango + Wheat) had the highest (103.4 cm) in Rudauli. Agri-Silviculture (AS) (Eucalyptus + Wheat) recorded the tallest average tree height (12.58 m) in Sohawal and AS (Eucalyptus + Mustard) was the tallest in Rudauli (16.10 m). The highest basal area was found in AH (Mango + Mustard) (0.091 m3) in Sohawal and AH (Mango + Wheat) (0.085 m3) in the Rudauli block. Volume peaked in AH (Mango + Wheat) (0.67 m3) in Sohawal and AS (Eucalyptus + Mustard) (0.68 m3) in Rudauli. Above-ground biomass (AGB) was highest in AS (Eucalyptus + Wheat) (17.48 Mg h-1) in Sohawal and AS (Eucalyptus + Mustard) (31.87 Mg h-1) in Rudauli. Below-ground biomass (BGB) followed similar trends, with 4.37 Mg h-1 and 7.97 Mg h-1 respectively. The highest total carbon storage was observed in AS (Eucalyptus + Wheat) (10.92 Mg h-1) in Sohawal and AS (Eucalyptus + Mustard) (19.92 Mg h-1) in Rudauli. Soil organic carbon (SOC) ranged from 0.21 % to 0.32 %, with the Teak + Wheat AS system recording the highest SOC (0.31–0.32 %) in both blocks. Overall, the findings underscore the carbon sequestration potential of agroforestry systems, supporting their role in sustainable land use, increasing species diversity and climate change mitigation as well as providing economic returns.