Skip to main navigation menu Skip to main content Skip to site footer

Research Articles

Vol. 13 No. sp1 (2026): Recent Advances in Agriculture

Evaluation of sweet flag (Acorus calamus L.) rhizome for its chemical properties and their effect on growth and seed longevity in field bean (Lablab purpureus L.)

DOI
https://doi.org/10.14719/pst.12667
Submitted
19 November 2025
Published
15-02-2026

Abstract

Sweet flag (Acorus calamus L.) is known for its pesticidal and seed-quality–enhancing properties due to its essential oil richness. The study quantified compounds in sweet flag rhizomes and assessed their effectiveness in improving field bean (var. HA-5) under field and laboratory conditions. Essential oil was extracted by hydro-distillation and quantified using gas chromatography (GC). A foliar spray experiment was conducted using a randomised complete block design (RCBD) to assess the effects of sweet flag rhizome powder, rhizome oil and a chemical check on crop growth, pest and disease incidence. A 9-month storage study was conducted under laboratory conditions to evaluate seed storability. The essential oil yield aveaged 0.29 % and was dominated by β-asarone. Results indicated that moderate concentrations of sweet flag rhizome powder and oil significantly improved seed emergence, plant growth- and plant stand, while also reducing pest and disease incidence. Seed treatment with rhizome powder at 10 g kg-1 consistently maintained higher seed quality during storage. Sweet flag rhizome-based treatments showed strong potential as eco-friendly options to enhance field performance and seed storability.

References

  1. 1. Sadohara R, Cichy K, Thompson H, Uebersax MA, Siddiq M, Wiesinger J. Nutritional attributes, health benefits, consumer perceptions and sustainability impacts of whole pulses and pulse flour-based ingredients. Crit Rev Food Sci Nutr. 2026;66(5):988–1011. https://doi.org/10.1080/10408398.2025.2538544
  2. 2. Ingle SM, Devmore JP, Bhave SG, Palshetkar MG, Thorat BS. Genetic variability for yield and yield attributing traits in F5 generation of lablab bean (Lablab purpureus L. Sweet) genotypes. Int J Curr Microbiol App Sci. 2020;9(4):466-75. https://doi.org/10.20546/ijcmas.2020.904.055
  3. 3. Pratheeksha P, Hegde K. A brief review on botanical description, medicinal uses and pharmacological actions of Lablab purpureus. Int J Res Rev. 2022;9(4):230-6. https://doi.org/10.52403/ijrr.20220428
  4. 4. Shukla UN, Mishra ML. Present scenario, bottlenecks and expansion of pulse production in India: A review. Legume Res. 2020;43(4):461–69.
  5. 5. Department of Agriculture & Farmers Welfare. Annual reports [Internet]. New Delhi: Government of India, Ministry of Agriculture & Farmers Welfare; 2026 [cited 2026 Feb 12]. Available from: https://agriwelfare.gov.in/en/Annual.
  6. 6. Kalpna, Hajam YA, Kumar R. Management of stored grain pest with special reference to Callosobruchus maculatus, a major pest of cowpea: a review. Heliyon. 2022;8(1):e08703. https://doi.org/10.1016/j.heliyon.2021.e08703
  7. 7. Paikaray SS, Satapathy SN, Sahoo BK. Estimation of yield loss due to pulse beetle, Callosobruchus chinensis (L.) on different mung bean cultivars. Pharma Innov J. 2022;11(3):924-7.
  8. 8. Lokesh GB. Sweet flag (Acorus calamus): cultivation and economic aspects. Indian J Nat Prod Resour. 2004;3(1):19–21.
  9. 9. Mssillou I, Agour A, Allali A, Saghrouchni H, Bourhia M, El Moussaoui A, et al. Antioxidant, antimicrobial and insecticidal properties of a chemically characterized essential oil from the leaves of Dittrichia viscosa L. Molecules. 2022;27(7):2282. https://doi.org/10.3390/molecules27072282
  10. 10. Khwairakpam AD, Damayenti YD, Deka A, Monisha J, Roy NK, Padmavathi G, et al. Acorus calamus: a bio-reserve of medicinal values. J Basic Clin Physiol Pharmacol. 2018;29(2):107-22. https://doi.org/10.1515/jbcpp-2016-0132
  11. 11. Aryal S, Poudel A, Kafle K, Aryal LN. Insecticidal toxicity of essential oil of Nepalese Acorus calamus against Sitophilus zeamais. Heliyon. 2023;9(11):e22130. https://doi.org/10.1016/j.heliyon.2023.e22130
  12. 12. Wang A, Zhou Y, Fu X, Wang X, Cheng Y, Zhang Y, et al. Structural derivatives of β-asarone from Acorus calamus Linn. as insecticide candidates and the insecticidal mechanism against small brown planthopper. Agronomy. 2024;14(10):2420. https://doi.org/10.3390/agronomy14102420
  13. 13. Lee JY, Lee JY, Yun BS, Hwang BK. Antifungal activity of β-asarone from rhizomes of Acorus gramineus. J Agric Food Chem. 2004;52(4):776-80. https://doi.org/10.1021/jf035204o
  14. 14. Liu XC, Zhou LG, Liu ZL, Du SS. Identification of insecticidal constituents of the essential oil of Acorus calamus rhizomes against Liposcelis bostrychophila Badonnel. Molecules. 2013;18(5):5684-96. https://doi.org/10.3390/molecules18055684
  15. 15. Prakash M, Georgin Ophelia A, Sathiya Narayanan G. Cumulative effect of botanical seed pelleting and foliar spray on morpho physiological, leaf chlorophyll, gas exchange and yield parameters in black gram. Legume Res. 2021;44(4):425-30.
  16. 16. Veerappan V, Ranganathan U, Mannar J. Effect of organic foliar spray with pulse sprout extract on seed yield and quality of rice (Oryza sativa). J Plant Nutr. 2019;42(8):900–14. https://doi.org/10.1080/01904167.2019.1567764
  17. 17. Nivethadevi P, Swaminathan C, Kannan P, Tamilselvi E. Seed fortification and foliar spraying with Moringa oleifera leaf extract enhances yield and yield attributes in blackgram (Vigna mungo L. Hepper). Legume Res. 2022;45(3).
  18. 18. Elluru S, Tiwari R. Field efficacy of botanical extracts against pod borers on pigeon pea, Cajanus cajan (L.) Millsp. Ecol Environ Conserv. 2024;30 Suppl(S255-60). https://doi.org/10.53550/EEC.2024.v30i04s.046
  19. 19. Muhammad A, Kashere MA. Neem (Azadirachta indica): an eco-friendly botanical insecticide for managing insect pests – a review. FUDMA J Sci. 2020;4(4):484–91. https://doi.org/10.33003/fjs-2020-0404-506
  20. 20. Bhalekar NB, Shelar VR, Mane TV, Bhingarde MT. Impact of pre-harvest spray of insecticides and botanicals on seed yield to control field infestation of pulse beetle in mung bean. Pharma Innov J. 2023;12(1):2761-4.
  21. 21. Yilma A, Abera M, Bekele B, Alemu T. Prevalence, pathogen diversity and management practices of chickpea Fusarium wilt in Ethiopia. CABI Rev. 2025;20(1):0043.
  22. 22. Patil SB, Goyal A, Chitgupekar SS, Kumar S, El-Bouhssini M. Sustainable management of chickpea pod borer. A review. Agron Sustain Dev. 2017;37:20. https://doi.org/10.1007/s13593-017-0428-8
  23. 23. Rajput SB, Tonge MB, Karuppayil SM. Traditional uses and pharmacological profile of Acorus calamus and other Acorus species. Phytomedicine. 2014;21(3):268–76. https://doi.org/10.1016/j.phymed.2013.09.020
  24. 24. Kumari S, Chandel SR, Atri S, Guleria S, Bhardwaj I, Rolta R. The therapeutic properties and applications of Acorus calamus (sweet flag): a review. Asian J Microbiol Biotechnol Environ Sci. 2022;24(1):122-136. https://doi.org/10.53550/AJMBES.2022.v24i01.022
  25. 25. Bhosle S, Ingle P. Conservation status, ethnobotanical characteristics and biological studies of Acorus calamus L. J Exp Agric Int. 2025;47(3):308–18. https://doi.org/10.9734/jeai/2025/v47i33337
  26. 26. Khanal D, Neupane SB, Bhattarai A, Khatri-Chhetri S, Nakarmi N, Sapkota S, et al. Evaluation of botanical powders for the management of rice weevil (Sitophilus oryzae L. Coleoptera: Curculionidae) in Rupandehi, Nepal. Adv Agric. 2021;2021:8878525. https://doi.org/10.1155/2021/8878525
  27. 27. Surabhi VK, Gouda R, Nethra N. Influence of seed treatment with nanoparticles on seed quality and storability of pigeon pea cv. BRG-2. Int J Chem Stud. 2021;9(1):3645–51. https://doi.org/10.22271/chemi.2021.v9.i1ay.11799
  28. 28. Sagili JL, Roopa Bai RS, Sharanagouda H, Ramachandra CT, Sushila Nadagouda S. Effect of zinc oxide nanoparticles on pulse beetle (Callosobruchus maculatus) (Col.: Chrysomelidae) in greengram. J Entomol Zool Stud. 2020;8(4):297-300.

Downloads

Download data is not yet available.