Research Articles
Vol. 13 No. sp5 (2026): Recent Advances in Agriculture
Impact of bioformulations on growth, yield and quality parameters of field-grown lettuce (Lactuca sativa L.)
Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara 144 411, Punjab, India
Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara 144 411, Punjab, India
Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara 144 411, Punjab, India
Abstract
The heavy reliance on fertilisers in crop cultivation has led to soil degradation and human health concerns, particularly in the cultivation of salad crops, where the leaves are consumed fresh. This necessitates the adoption of organic and bio-based sustainable bioformulations. The field experiment was conducted during the rabi 2024–2025 at the Department of Horticulture, Lovely Professional University, Punjab, to investigate the effects of bioformulations on growth, yield and quality parameters of lettuce (Lactuca sativa L.) cv. Green Wave. The trial was conducted using a randomised complete block design (RCBD) with 16 different treatments, each replicated 3 times. Statistical significance was assessed using analysis of variance (ANOVA) and means were compared using critical difference (CD) at p ≤ 0.05. The investigation revealed that T6 (Arka microbial consortium at 5 kg ha-1+ local microbial consortium at 5 kg ha-1, each mixed at 20 g L-l of water) recorded the highest values for growth and yield parameters i.e., plant height, number of leaves, leaf area, leaf length, leaf width, leaf fresh weight, leaf dry weight, days taken to maturity, yield per pot, yield per hectare followed by T11 (Arka vegetable special at 5 kg ha-1, mixed at 20 g L-1 + seaweed extract 1.5 L ha-1) whereas T13 (Arka microbial consortium at 5 kg ha-1 + neem seed kernel extract 15 L ha-1) recorded higher values for quality parameters such as calcium (Ca), iron (Fe), beta-carotene and vitamin C, followed by T₆.
References
- 1. Food and Agriculture Organisation of the United Nations. World food and agriculture: statistical yearbook 2023. Rome: FAO; 2023. https://doi.org/10.4060/cc8166en
- 2. Bahadur A, Singh J, Singh KP, Upadhyay AK, Rai M. Effect of organic amendments and biofertilisers on growth, yield and quality attributes of Chinese cabbage (Brassica pekinensis). Indian J Agric Sci. 2006;76:596–608.
- 3. Meena RS, Kumar S, Datta R, Lal R, Vijayakumar V, Yadav GS. Impact of agrochemicals on soil microbiota and management: a review. Land. 2020;9(2):34. https://doi.org/10.3390/land9020034
- 4. Dangi SR, Poudel R, Dahal KR. Examining the effect of agrochemicals on soil microbiological activity, micronutrient availability and uptake by maize (Zea mays L.) plants. Agronomy. 2024;14(6):1321. https://doi.org/10.3390/agronomy14061321
- 5. Aamir M, Rai KK, Zehra A, Dubey MK, Kumar S, Shukla V, et al. Microbial bioformulation-based plant biostimulants: a plausible approach toward next generation of sustainable agriculture. In: Microbial Endophytes. 2020. p. 195–224. https://doi.org/10.1016/B978-0-12-819654-0.00008-9
- 6. Khan MA, Singh D, Bhattacharya S, Singh N. Recent trends in microbial bioformulations and their role in sustainable agriculture. Front Sustain Food Syst. 2023;7:1184023. https://doi.org/10.3389/fsufs.2023.1184023
- 7. Novinscak A, Filion M, Germida JJ. Bioformulation strategies to improve the performance of plant beneficial microbes. Front Plant Sci. 2020;11:1205. https://doi.org/10.3389/fpls.2020.01205
- 8. Krishnaprabu S. Liquid microbial consortium: a potential tool for sustainable soil health. J Pharmacogn Phytochem. 2020;9(2):2191–9. https://doi.org/10.22271/phyto.2020.v9.i2aj.11182
- 9. Arora NK, Fatima T. Role of plant growth promoting microbes in managing sustainability of stressed agroecosystems. Environ Sustain. 2022;5(1):1–3. https://doi.org/10.1007/s42398-022-00222-z
- 10. Singh A, Kumari R, Yadav AN, Mishra S, Sachan A, Sachan SG. Tiny microbes, big yields: microorganisms for enhancing food crop production for sustainable development. In: New and Future Developments in Microbial Biotechnology and Bioengineering. 2020. p. 1–15. https://doi.org/10.1016/B978-0-12-820526-6.00001-4
- 11. Kashyap AS, Shukla P, Nain L. Screening microbial inoculants and their interventions for plant growth promotion: a review. Front Microbiol. 2023;14:1197376. https://doi.org/10.3389/fmicb.2023.1197376
- 12. Vendan RT, Thangaraju M. Development and standardisation of liquid formulation for Azospirillum bioinoculant. Indian J Microbiol. 2006;46:379–84.
- 13. Mącik M, Gryta A, Frąc M. Biofertilisers in agriculture: an overview on concepts, strategies and effects on soil microorganisms. Adv Agron. 2020;162:31–87. https://doi.org/10.1016/bs.agron.2020.02.001
- 14. Mahanty T, Bhattacharjee S, Goswami M, Bhattacharyya P, Das B, Ghosh A, et al. Biofertilisers: a potential approach for sustainable agriculture development. Environ Sci Pollut Res Int. 2017;24:3315–35. https://doi.org/10.1007/s11356-016-8104-0
- 15. Asoegwu CR, Awuchi CG, Nelson-Kalu CT, Orji CG, Nwosu OU, Egbufor UC. A review on the role of biofertilisers in reducing soil pollution and increasing soil nutrients. Himalayan J Agric. 2020;1(1):34–8. https://doi.org/10.47310/hja.2020.v01i01.006
- 16. Kathula KVP, Manohar KA, Sagar L. Bio-fertiliser: a sustainable way for soil amelioration. J Crop Weed. 2023;19(3):93–9. https://doi.org/10.22271/09746315.2023.v19.i3.1746
- 17. Sheoran OP, Tonk DS, Kaushik LS, Hasija RC, Pannu RS. Statistical software package for agricultural research workers. Hisar: CCS Haryana Agricultural University; 1998.
- 18. Rouphael Y, Carillo P, Colla G, Fiorentino N, Sabatino L, El-Nakhel C, et al. Evaluation of combined applications of Trichoderma virens and a biopolymer-based biostimulant in agronomic, physiological and qualitative properties of lettuce under variable N regimes. Agronomy. 2020;10:196. https://doi.org/10.3390/agronomy10020196
- 19. Oliveira TML, Pires JSB, Oliveira VS, Jeveaux Machado AJC, Fernandes AA, Arantes LDO, et al. Potential of the use of biostimulants in lettuce production. Plants. 2025;14(15):2416. https://doi.org/10.3390/plants14152416
- 20. İkiz B, Dasgan HY, Balik S, Kusvuran S, Gruda NS. The use of biostimulants as a key to sustainable hydroponic lettuce farming under saline water stress. BMC Plant Biol. 2024;24(1):808. https://doi.org/10.1186/s12870-024-05520-8
- 21. Colla G, Rouphael Y, Cardarelli M, Tullio M, Rivera CM, Rea E. Co-inoculation of Glomus intraradices and Trichoderma atroviride acts as a biostimulant to promote growth, yield and nutrient uptake of vegetable crops. J Sci Food Agric. 2015;95(8):1706–15. https://doi.org/10.1002/jsfa.6875
- 22. Kim C, van Iersel MW. Morphological and physiological screening to predict lettuce biomass production in controlled environment agriculture. Remote Sens. 2022;14(2):316. https://doi.org/10.3390/rs14020316
- 23. Minello LV, Kuntzler SG, Berghahn E, Dorneles LT, Ricachenevsky FK, Sperotto RA. Nanotechnology-driven biofortification of Fe, Zn and Se in edible plants. J Nanobiotechnol. 2025;23(1):669. https://doi.org/10.1186/s12951-025-03746-8
- 24. Rouphael Y, Carillo P, Garcia-Perez P, Cardarelli M, Senizza B, Miras-Moreno B, et al. Plant biostimulants from seaweeds or vegetal proteins enhance the salinity tolerance in greenhouse lettuce by modulating plant metabolism in a distinctive manner. Sci Hortic (Amsterdam). 2022;305:111368. https://doi.org/10.1016/j.scienta.2022.111368
Downloads
Download data is not yet available.