Skip to main navigation menu Skip to main content Skip to site footer

Research Articles

Vol. 13 No. sp5 (2026): Recent Advances in Agriculture

Assessment of sheath blight resistance in rice landraces using disease progression metrics and machine learning

DOI
https://doi.org/10.14719/pst.15304
Submitted
28 April 2026
Published
24-07-2026

Abstract

Sheath blight (ShB), caused by Rhizoctonia solani, is a major constraint in rice (Oryza sativa L.) production, leading to considerable yield losses. The objective of the present study was to assess sheath blight resistance in rice landraces based on the disease progression parameters and machine learning methods. Field screening was conducted under inoculated conditions and disease progression was monitored from 10–70 days after inoculation using disease severity (DS), percent disease index (PDI) and area under disease progress curve (AUDPC). Machine learning models - linear regression, decision tree, random forest and gradient boosting were employed to predict DS of sheath blight in rice landraces based on disease progression parameters and their predictive performances were compared using R², root mean square error (RMSE) and classification-based evaluation metrics. Among the landraces screened, NLR 33892 and Swarna Swoubhagya showed resistance against R. solani, while the majority were susceptible or highly susceptible. Both DS and PDI increased progressively over time and showed a strong positive correlation across assessment stages, confirming consistent disease development. Linear regression performed the best with the highest coefficient of determination (R2 = 0.8178) and the lowest RMSE = 3.852, followed by decision tree (R2 = 0.8077), random forest (R2 = 0.8010) and gradient boosting (R2 = 0.7899). The analysis of confusion matrices further supported the better consistency of classification of linear regression.

References

  1. 1. Food and Agriculture Organization of the United Nations. World food and agriculture – Statistical yearbook 2023. Rome: FAO; 2023. https://doi.org/10.4060/cc8166en
  2. 2. Rajkumar D, Rao GS, Tirupathi B, Rodriguez GH. Activities of antioxidant enzymes in six rice (Oryza sativa L.) varieties at seedling stage under increasing salinity stress. International Journal of Economic Plants. 2019;9(1):49–58.
  3. 3. Reddy V, Mahantashivayogayya K, Pramesh D, Diwan JR, Tembhurne BV. Pheno-genotypic screening of medium slender rice genotypes for bacterial leaf blight disease resistance. International Journal of Bio-resource and Stress Management. 2023;14(3):422–8. https://doi.org/10.23910/1.2023.3378a
  4. 4. Anonymous. Rice statistics. Market Data Report 2024. World Metrics. https://worldmetrics.org/rice-statistics
  5. 5. Singh R, Sunder S, Kumar P. Sheath blight of rice: current status and perspectives. Indian Phytopathology. 2016;69(4):340–51.
  6. 6. Kumar AB, Siddhartha D. Journal of Plant Protection Research. 2024;64(3):209–33. https://doi.org/10.24425/jppr.2024.151260
  7. 7. Ou SH. Rice diseases. 2nd ed. Kew, Surrey: Commonwealth Mycological Institute; 1985. 380 p. https://doi.org/10.1017/S0014479700017245
  8. 8. Molla KA, Karmakar S, Molla J, Bajaj P, Varshney RK, Datta SK, et al. Understanding sheath blight resistance in rice: the road behind and the road ahead. Plant Biotechnology Journal. 2020;18(4):895–915. https://doi.org/10.1111/pbi.13312
  9. 9. Groth DE. Effects of cultivar resistance and single fungicide application on rice sheath blight, yield and quality. Crop Protection. 2008;27(8):1125–30. https://doi.org/10.1016/j.cropro.2008.01.010
  10. 10. Koshariya A, Kumar I, Pradhan A, Shinde U, Verulkar SB, Agrawal T, et al. Identification of quantitative trait loci (QTL) associated with sheath blight tolerance in rice. Indian Journal of Genetics and Plant Breeding. 2018;78:196–201. https://doi.org/10.5958/0975-6906.2018.00025.1
  11. 11. Kumar RBP, Reddy KRN, Rao KS. Sheath blight disease of Oryza sativa and its management by biocontrol and chemical control in vitro. Electronic Journal of Environmental, Agricultural and Food Chemistry. 2009;8:639–46.
  12. 12. Shamim MD, Kumar D, Srivastava D, Pandey P, Singh KN. Evaluation of major cereal crops for resistance against Rhizoctonia solani under greenhouse and field conditions. Indian Phytopathology. 2014;67(1):2–6.
  13. 13. Timsina A, Thera UK, Ramasamy N. Phenotypic screening of F3 rice population resistance associated with sheath blight disease. International Journal of Bio-resource and Stress Management. 2022;13(5):527–34. https://doi.org/10.23910/1.2022.2877
  14. 14. Uppala L, Zhou X. Rice sheath blight. Plant Health Instructor. 2018;18(1). https://doi.org/10.1094/PHI-I-2018-0403-01
  15. 15. Willocquet L, Savary S. Resistance to rice sheath blight (Rhizoctonia solani Kühn) [(teleomorph: Thanatephorus cucumeris)]: current status and perspectives. Euphytica. 2011;178(1):1–22. https://doi.org/10.1007/s10681-010-0296-7
  16. 16. Shiobara F, Ozaki H, Sato H, Kojima Y, Masahiro M. Mapping and validation of QTLs for rice sheath blight resistance. Breeding Science. 2013;63:301–8. https://doi.org/10.1270/jsbbs.63.301
  17. 17. Chandra S, Singh HK, Kumar P, Yadav N. Screening of rice (Oryza sativa L.) genotypes for sheath blight (Rhizoctonia solani) in changing climate scenario. Journal of AgriSearch. 2016;3(2):130–2. https://doi.org/10.21921/jas.v3i2.11275
  18. 18. Goswami SK, Singh V, Kashyap PL, Singh PK. Morphological characterization and screening for sheath blight resistance using Indian isolates of Rhizoctonia solani AG-IIA. Indian Phytopathology. 2019;72(1):107–24. https://doi.org/10.1007/s42360-018-0103-2
  19. 19. Pavani SL, Singh V, Goswami S, Singh PK. Screening for novel rice sheath blight-resistant germplasm and their biochemical characterization. Indian Phytopathology. 2020;73:1–6. https://doi.org/10.1007/s42360-020-00284-1
  20. 20. Tejaswini KLY, Krishnam R, Kumar R, Mohammad LA, Ramakumar PV, Sayanarayana PV, et al. Screening of rice F5 families for sheath blight and bacterial leaf blight. Journal of Rice Research. 2016;9(1):4–10.
  21. 21. Bal A, Samal P, Chakraborti M, Mukherjee AK, Ray S, Molla KA, et al. Stable quantitative trait locus (QTL) for sheath blight resistance from rice cultivar CR 1014. Euphytica. 2020;216(11):182. https://doi.org/10.1007/s10681-020-02702-x
  22. 22. Upadhyaya R, Danilevicz MF, Dolatabadian A, Neik TX, Zhan F, Al-Mamun HA, et al. Genome-based plant disease resistance prediction using machine learning. Plant Pathology. 2024;73(9):2298–309. https://doi.org/10.1111/ppa.13958
  23. 23. Chen J, Xuan Y, Yi J, Xino G, Yuan DP, Li D. Progress in rice sheath blight resistance research. Frontiers in Plant Science. 2023;14:1141697. https://doi.org/10.3389/fpls.2023.1141697
  24. 24. Boysen M, Borja M, del Moral C, Salazar O, Rubio V. Identification at strain level of Rhizoctonia solani AG 4 isolates by direct sequence of asymmetric PCR products of the ITS regions. Current Genetics. 1996;29(2):174–81. https://doi.org/10.1007/s002940050033
  25. 25. Walther G, Pawlowska J, Alastruey-Izquierdo A, Wrzosek M, Rodriguez-Tudela JL, Dolatabadian S, et al. DNA barcoding in Mucorales, an inventory of biodiversity. Persoonia – Molecular Phylogeny and Evolution of Fungi. 2013;30(1):11–47. https://doi.org/10.3767/003158513X665070
  26. 26. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications. London: Academic Press; 1990. p. 315–22. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  27. 27. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution. 1987;4:406–25.
  28. 28. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 1985;39:783–91. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
  29. 29. Singh A, Singh US, Singh V, Zeigler RS, Hill JE, Singh VP, et al. Rhizoctonia solani in rice–wheat system. Journal of Mycology and Plant Pathology. 2000;30(3):343–9.
  30. 30. Madden LV, Hughes G, Van Den Bosch F. The study of plant disease epidemics. St Paul (MN): American Phytopathological Society; 2007.
  31. 31. HB A, Kumar NK, Kumar LV, Ashoka KR, Pankaja NS, Mallikarjuna N. Identification of resistant sources against sheath blight of rice caused by Rhizoctonia solani Kuhn. International Journal of Bio-Resource and Stress Management. 2025;16(3):1–13. https://doi.org/10.23910/1.2025.6030
  32. 32. Yasin A, Kashyap A, Dowarah B, Dey J, Bharali S, Nath BC, et al. A reliable in planta inoculation and antifungal screening protocol for Rhizoctonia solani-induced sheath blight in rice. Bio-protocol. 2025;15(21). https://doi.org/10.21769/BioProtoc.5491
  33. 33. Yang X, Gu X, Ding J, Yao L, Gao X, Zhang M, et al. Gene expression analysis of resistant and susceptible rice cultivars to sheath blight after inoculation with Rhizoctonia solani. BMC Genomics. 2022;23(1):278. https://doi.org/10.1186/s12864-022-08524-6
  34. 34. Senapati M, Tiwari A, Sharma N, Chandra P, Bashyal BM, Ellur RK, et al. Rhizoctonia solani Kühn pathophysiology: status and prospects of sheath blight disease management in rice. Frontiers in Plant Science. 2022;13:881116. https://doi.org/10.3389/fpls.2022.881116

Downloads

Download data is not yet available.