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Plant Science Today (PST)

Research Articles

Vol. 12 No. 1 (2025)

In vitro biological activity of seed oil of Withania coagulans and synthesis of catalyst from residual biomass

DOI
https://doi.org/10.14719/pst.3288
Submitted
22 January 2024
Published
21-12-2024 — Updated on 01-01-2025
Versions

Abstract

Withania coagulans is a well-known medicinal plant reported to possess numerous biological activities. However, the plant remains underutilized compared to other medicinal plants due to lack of clinical and analytical studies. In the present study, in vitro biological activities of oil extracted from seeds of W. coagulans were studied along with synthesis and characterization of catalyst from residue obtained post oil extraction. The extracted oil exhibited DPPH-radical scavenging activity, protein denaturation activity and in vitro -? amylase inhibitory activity. These activities were comparable to the corresponding activities of standard compounds indicating diverse medicinal potential of the oil. The residue obtained post oil extraction was utilized for synthesis of catalyst. The synthesized catalyst was characterized to identify the nature of the catalyst.   Catalyst synthesized from residue was found to be silica-based catalyst, crystalline in nature and EDX mapping revealed catalyst to comprise of C, O, Na, Si, P and Ca.  Interpretation of results obtained depict potential utilization of W. coagulans for diversified traditional as well as industrial application.

References

  1. Azhar MF, Naseer U, Aziz A. Antioxidant and phytochemical composition of leaves, stem and root extracts of Withania coagulans and Withania somnifera. J Medic Spice Plants. 2020;24(1):27-30.
  2. Jain R, Kachhwaha R, Kothari SL. Phytochemistry, pharmacology and biotechnology of Withania somnifera and Withania coagulans: A Review. J Medic Plants Res. 2015;6(41):5388-99. https://doi.org/10.5897/JMPR12.704.
  3. Sharma N, Sachdeva P, Dhiman M, Koshy EP. Comparative evaluation of in vitro regeneration potential of seeds of W. somnifera and W. coagulans. Biotechnology International. 2015;8(1):21-33.
  4. Nath B, Pranjal K, Bipul D, Sanjay B. Highly efficient renewable heterogeneous base catalyst derived from waste Sesamum indicum plant for synthesis of biodiesel. Renewable Energy. 2020;151:295-310. https://doi.org/10.1016/j.renene.2019.11.029
  5. Nath B, Bipul Das, Pranjal Kalita, Sanjay Basumatary. Waste to value addition: Utilization of waste Brassica nigra plant derived novel green heterogeneous base catalyst for effective synthesis of biodiesel. J Cleaner Production. 2019;239:118112. https://doi.org/10.1016/j.jclepro.2019.118112
  6. Hargreaves JSJ. Catalysts derived from waste materials. Catalysis. 2018;30:1-20. DOI: 10.1039/9781788013048-00001, E-ISBN: 978-1-78801-304-8.
  7. Sarai NS, Levin BJ, Roberts JM, et al., Biocatalytic transformations of silicon-the other group 14 element. ACS Central Sci. 2021;7(6):944-53. https://doi.org/10.1021/acscentsci.1c00182
  8. Fang H, Xiaodong W, Dezhen W. Phase-change characteristics and thermal performance of form-stable n-alkanes/silica composite phase change materials fabricated by sodium silicate precursor. Renewable Energy. 2015;74:689-98. https://doi:10.1016/j.renene.2014.08.079
  9. Dani NAB, Asep S, Takashi O, Ryohei U, Kikuo O. Size- and charge-controllable polystyrene spheres for templates in the preparation of porous silica particles with tunable internal hole configurations. Chemical Engineering J. 2014;256:421-30. https://doi:10.1016/j.cej.2014.07.005
  10. Raghav P, Choudhary P, Singh A, Sharma R, Sharma N. Extraction and characterization of oil from seeds of the medicinal plant Withania coagulans (Stocks) Dunal (Doda paneer). Plant Sci Today. 2022;9(3):698-704. https://horizonepublishing.com/journals/index.php/PST/article/view/1649
  11. Williams LAD, O'Connar A, Latore L, et al. The in vitro anti-denaturation effects induced by natural products and non-steroidal compounds in heat treated (Immunogenic) bovine serum albumin is proposed as a screening assay for the detection of anti-inflammatory compounds, without the use of animals, in the early stages of the drug discovery process. West Indian Med J. 2008;57(4).
  12. Borah A, Paw M, Gogoi R, et al. Chemical composition, antioxidant, anti-inflammatory, anti-microbial and in-vitro cytotoxic efficacy of essential oil of Curcuma caesia Roxb. leaves: An endangered medicinal plant of North East India. Indust Crops and Products. 2019;129:448-54. https://doi.org/10.1016/j.indcrop.2018.12.035
  13. Banerjee A, Maji B, Mukherjee S, Chaudhuri K, Seal T. In vitro antidiabetic and anti-oxidant activities of methanol extract of Tinospora sinensis. J Applied Biology and Biotechnology. 2017;5(3):1-6.
  14. Asraoui F, Kounnoun A, Cadi HE, et al. Phytochemical investigation and antioxidant activity of Globularia alypum L. Molecules. 2021;26(3). https://doi.org/10.3390/molecules26030759
  15. Phromnoi K, Sinchaiyakij P, Khanaree C, et al. Anti-inflammatory and antioxidant activities of medicinal plants used by traditional healers for antiulcer treatment. Scientia Pharma. 2019;87(3). https://doi.org/10.3390/scipharm87030022
  16. Fyad K, Belboukhari N, Hadj-Khelil AOE, et al. Analgesic and anti-inflammatory activity of aqueous extract of Bubonium graveolens. Biomed Res Therapy. 2020;7(9):4002-09. https://doi.org/10.15419/bmrat.v7i9.632
  17. Sareen A, Sultana S, Akbar S, Rizwana D, Naz G, Akhter N, et al. In vitro evaluation of Withania coagulans for its thrombolytic, anti-oxidant, anti-bacterial and cytotoxic potential. Kurdish Studies. 2024;12(2):6706-23. https://doi.org/10.53555/ks.v12i2.2894
  18. Nishtha R, Agarwal A, Chaturvedi. In vitro study on total phenols, flavonoids content and DPPH activity of Withania species. Int J Environment Agriculture Biotech. 2017;2(2). http://dx.doi.org/10.22161/ijeab/2.2.49
  19. Brangule A, Sukele R, Bandere D. Herbal medicine characterization perspectives using advanced FTIR sample techniques – diffuse reflectance (DRIFT) and photoacoustic spectroscopy (PAS). Front Plant Sci. 2020;11. https://doi.org/10.3389/fpls.2020.00356
  20. Siregar AGA, Manurung R, Taslim T. Synthesis and characterization of sodium silicate produced from corncobs as a heterogeneous catalyst in biodiesel production. Indones J Chem. 2021;21(1):88-96. https://doi.org/10.22146/ijc.53057
  21. Gopal NO, Narasimhulu KV, Rao JL. EPR, optical, infrared and Raman spectral studies of actinolite mineral. Spectrochimica Acta Part A. 2004;60(11):2441-48. https://doi:10.1016/j.saa.2003.12.021
  22. Coates J. Interpretation of infrared spectra, a practical approach. Encyclopaedia of Anal Chem. 2000;12:10815-37.
  23. Behbahani BA, Falah F, Arab FL. Chemical composition and antioxidant, antimicrobial and antiproliferative activities of Cinnamomum zeylanicum bark essential oil. Evidence-Based Complement Alter Medic. 2020. https://doi.org/10.1155/2020/5190603
  24. Ibrahim NA, Rashid U, Choong TSY, et al. Synthesis of nano magnetic sulphonated impregnated Ni/Mn/Na2SiO3 as catalyst for esterification of palm fatty acid distillate. Royal Society of Chem Adv. 2020;10:6098-108. https://doi.org/10.1039/C9RA08115
  25. Choudhary P, Sharma R, Kumar V, Singh A. Synthesis, characterization and catalytic activity of bio-MCM-41 for production of bio crude oil via pyrolysis of rice straw. Waste and Biomass Valorization. 2023;1-14. 10.1007/s12649-023-02124-5
  26. Devine PN, Howard RM, Kumar R, et al. Extending the application of biocatalysis to meet the challenges of drug development. Nature Reviews Chem. 2018. https://doi.org/10.1038/s41570-018-0055-1

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