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Plant Science Today (PST)

Research Articles

Vol. 12 No. 2 (2025)

DNA barcode based cultivar authentication in Clitoria ternatea L. using rpoB and rbcL conserve regions

DOI
https://doi.org/10.14719/pst.4403
Submitted
20 July 2024
Published
31-03-2025 — Updated on 13-04-2025
Versions

Abstract

Clitoria ternatea (C. ternatea), generally known as Shankhpushpi, is an essential substance in Ayurvedic medicine used for memory enhancement, and as a nootropic, tranquillizing, anti-stress, anxiolytic, anti-convulsant and sedative agent. Identification of the vegetative state of cultivars is a challenging action within the species. In the current study, 6 cultivars were recognized by using universally conserved sequences. DNA barcoding is based on molecular markers technology, typically used for diversity or species identification. To investigate 6 distinct C. ternatea L. varieties that were collected in and around Bhubaneswar, Odisha, we employed 9 conserved locus barcode locations: atpF-atpH, rpoB, matK, rpoC, psbK-psbI, trnH-psbA and rbcL. After PCR, gel electrophoresis yielded significantly distinct bands for 5 markers: rbcL, rpoB, atpF-atpH, trnH-psbA and psbK-psbI. Phylogenetic analysis and sequencing of the amplified bands using the rpoB and rbcL barcode primers revealed insertion and deletion, which were suggestive of consistent separation of all 6 cultivars. Consequently, these markers may be utilized to identify the cultivars of C. ternatea. These barcode markers may also be used to recognize different C. ternatea cultivars.

References

  1. Gupta GK, Chahal J, Bhatia M. Clitoria ternatea (L.): Old and new aspects. J Pharm Res. 2010 Nov;3(11):2610-14.
  2. Oguis GK, Gilding EK, Jackson MA, Craik DJ. Butterfly pea (Clitoria ternatea), a cyclotide-bearing plant with applications in agriculture and medicine. Front in Plant Sci. 2019 May 28;10:645. https://doi.org/10.3389/fpls.2019.00645
  3. Nurhasanah, Hindersah R, Suganda T, Concibido V, Sundari, Karuniawan A. The first report on the application of ISSR markers in genetic variance detection among butterfly pea (Clitoria ternatea L.) accession in North Maluku Province, Indonesia. Horticulturae. 2023 Sep 21;9(9):1059. https://doi.org/10.3390/horticulturae9091059
  4. Suarna IW, Wijaya IM. Butterfly pea (Clitoria ternatea L.: Fabaceae) and its morphological variations in Bali. J Trop Biodiversity and Biotech. 2021;6(2):63013. https://doi.org/10.22146/jtbb.63013
  5. Pfenninger M, Véla E, Jesse R, Elejalde MA, Liberto F, Magnin F, Martínez-Ortí A. Temporal speciation pattern in the western Mediterranean genus Tudorella P. Fischer, 1885 (Gastropoda, Pomatiidae) supports the Tyrrhenian vicariance hypothesis. Mol Phylogen and Evol. 2010 Feb 1;54(2):427-36. https://doi.org/10.1016/j.ympev.2009.09.024
  6. Ali Z, Ganie SH, Narula A, Sharma MP, Srivastava PS. Intra-specific genetic diversity and chemical profiling of different accessions of Clitoria ternatea L. Indus Crops and Prod. 2013 May 1;43:768-73. https://doi.org/10.1016/j.indcrop.2012.07.070
  7. Li Y, Kong D, Fu Y, Sussman MR, Wu H. The effect of developmental and environmental factors on secondary metabolites in medicinal plants. Plant Physiol and Biochem. 2020 Mar 1;148:80-89. https://doi.org/10.1016/j.plaphy.2020.01.006
  8. Naik A, Mishra SK, Nag A, Soren GK, Panda AK, Panda SK, et al. Cross-genera amplification of Cajanus spp. specific SSR markers in Clitoria ternatea (L.) and their application in genetic diversity studies. Physiol and Mol Bio of Plants. 2020 Dec;26(12):2371-90. https://doi.org/10.1007/s12298-020-00907-x
  9. Weerasinghe T, Perera D, Silva ND, Poogoda D, Swarnathilaka H. Butterfly pea: An emerging plant with applications in food and medicine. The Pharma Innova J. 2022;11(6):625-37.
  10. Ren JI, Lu Y, Qian Y, Chen B, Wu TA, Ji G. Recent progress regarding kaempferol for the treatment of various diseases. Experimental and Therapeutic Med. 2019 Oct 1;18(4):2759-76. https://doi.org/10.3892/etm.2019.7886
  11. Huntley AL. The health benefits of berry flavonoids for menopausal women: cardiovascular disease, cancer and cognition. Maturitas. 2009 Aug 20;63(4):297-301. https://doi.org/10.1016/j.maturitas.2009.05.005
  12. Nijveldt RJ, Van Nood EL, Van Hoorn DE, Boelens PG, Van Norren K, Van Leeuwen PA. Flavonoids: a review of probable mechanisms of action and potential applications. The American J Clinical Nutri. 2001 Oct 1;74(4):418-25. https://doi.org/10.1093/ajcn/74.4.418
  13. Devi KP, Malar DS, Nabavi SF, Sureda A, Xiao J, Nabavi SM, Daglia M. Kaempferol and inflammation: From chemistry to medicine. Pharmacol Res. 2015 Sep 1;99:1-0. https://doi.org/10.1016/j.phrs.2015.05.002
  14. Chavan SS, Jadhav RS, Kharat D, Mankar SD, Godge RK. Evaluation of analgesic activity and phytochemical screening of Clitoria ternatea Linn. British J Pharma Res. 2015 Jan 10;6(4):255-60. https://doi.org/10.9734/BJPR/2015/13593
  15. Jiji KN, Muralidharan P. Neuroprotective effects of Clitoria ternatea L. against propionic acid-induced behavior and memory impairment in autistic rat model. Future J Pharma Sci. 2021 Aug 21;7(1):163. https://doi.org/10.1186/s43094-021-00314-3
  16. Swathi KP, Jayaram S, Sugumar D, Rymbai E. Evaluation of anti-inflammatory and anti-arthritic property of ethanolic extract of Clitoria ternatea. Chinese Herb Med. 2021 Apr 1;13(2):243-49. https://doi.org/10.1016/j.chmed.2020.11.004
  17. Tunna T, Akter MS, Parvin M, Jilhaz M, Jahan S, Zaidul IS. A comparative in vivo study on Bambusa polymorpha, Mentha piperita and Clitoria ternatea as alternative anxiolytic. European J Med and Health Sci. 2020 May 22;2(3): https://doi.org/10.24018/ejmed.2020.2.3.266
  18. Lijon MB, Meghla NS, Jahedi E, Rahman MA, Hossain I. Phytochemistry and pharmacological activities of Clitoria ternatea. Inter J Nat and Social Sci. 2017 Jan;4(1):1-0.
  19. Mukherjee PK, Kumar V, Kumar NS, Heinrich M. The ayurvedic medicine Clitoria ternatea—From traditional use to scientific assessment. J Ethnopharma. 2008 Dec 8;120(3):291-301. https://doi.org/10.1016/j.jep.2008.09.009
  20. Abdi G, Singh S, Selvakumar S, Dhar SK, Mudgal G, Swaminathan P, et al. DNA barcoding and its applications. In: Advances in genomics: Methods and applications; 2024 Jul 31. pp. 91-117 https://doi.org/10.1007/978-981-97-3169-5_5
  21. Viáfara-Vega RA, Cárdenas-Henao H. Identification of Capsicum species from Colombia by DNA barcoding and high-resolution melting (HRM) analysis. Genetic Resour and Crop Evol. 2024 Sep 8;1-0. https://doi.org/10.1007/s10722-024-02097-x
  22. Hollingsworth PM, Graham SW, Little DP. Choosing and using a plant DNA barcode. PloS One. 2011 May 26;6(5):e19254. https://doi.org/10.1371/journal.pone.0019254
  23. Wu CS, Sudianto E, Hung YM, Wang BC, Huang CJ, Chen CT, et al. Genome skimming and exploration of DNA barcodes for Taiwan endemic cypresses. Scientific Reports. 2020 Nov 26;10(1):20650. https://doi.org/10.1038/s41598-020-77492-2
  24. Ferri E, Barbuto M, Bain O, Galimberti A, Uni S, Guerrero R, et al. Integrated taxonomy: traditional approach and DNA barcoding for the identification of filarioid worms and related parasites (Nematoda). Front in Zoology. 2009 Dec;6:1-2. https://doi.org/10.1186/1742-9994-6-1
  25. Shinwari ZK, Jan SA, Khalil AT, Khan AD, Ali M, Qaiser M, et al. Identification and phylogenetic analysis of selected medicinal plant species from Pakistan: DNA barcoding approach. Pak J Bot. 2018 Apr 1;50(2):553-60.
  26. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic acids Res. 1997 Sep 1;25(17):3389-402. https://doi.org/10.1093/nar/25.17.3389
  27. Bansal V, Harismendy O, Tewhey R, Murray SS, Schork NJ, Topol EJ, et al. Accurate detection and genotyping of SNPs utilizing population sequencing data. Genome Res. 2010 Apr 1;20(4):537-45. https://doi.org/10.1101/gr.100040.109
  28. Singh HK, Parveen I, Raghuvanshi S, Babbar SB. The loci recommended as universal barcodes for plants on the basis of floristic studies may not work with congeneric species as exemplified by DNA barcoding of Dendrobium species. BMC Res Notes. 2012 Dec;5:1-1. https://doi.org/10.1186/1756-0500-5-42
  29. Mehmood F, Shahzadi I, Waseem S, Mirza B, Ahmed I, Waheed MT. Chloroplast genome of Hibiscus rosa-sinensis (Malvaceae): comparative analyses and identification of mutational hotspots. Genomics. 2020 Jan 1;112(1):581-91. https://doi.org/10.1016/j.ygeno.2019.04.010

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