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Plant Science Today (PST)

Research Articles

Vol. 12 No. 1 (2025)

Melatonin application extends banana shelf life by delayed ripening

DOI
https://doi.org/10.14719/pst.6867
Submitted
23 December 2024
Published
02-03-2025 — Updated on 18-03-2025
Versions

Abstract

Bananas are among the worlds’ most economically significant fruit crops, valued for their distinctive flavour and aroma, despite being highly susceptible to rapid postharvest physiological deterioration. Melatonin, a non-toxic bioactive compound, regulates several plant physiological processes and is widely studied in postharvest management. With this background, the study investigated the effect of different concentrations of melatonin on three banana varieties, namely Grand Naine (AAA), Poovan (AAB) and Ney Poovan (AB). The fruits were stored under ambient (AS) (27 ± 2°C) and cold storage (CS) (17°C) with 90–95% relative humidity. Melatonin treatment (1000 µM) effectively reduced weight loss, retained firmness and total soluble solids, reduced ethylene production, enhanced chlorophyll retention and delayed starch degradation. Grand Naine showed the most pronounced effects, followed by Ney Poovan and Poovan. Overall, 1000 µM melatonin was the most effective treatment for extending banana shelf life and maintaining quality under ambient and cold storage conditions.

References

  1. Aghdam MS, Fard JR. Melatonin treatment attenuates postharvest decay and maintains nutritional quality of strawberry fruits (Fragaria× anannasa cv. Selva) by enhancing GABA shunt activity. Food Chem. 2017;221:1650–57. https://doi.org/10.1016/j.foodchem.2016.10.123
  2. Arnao MB, Hernández?Ruiz J. Protective effect of melatonin against chlorophyll degradation during the senescence of barley leaves. J Pineal Res. 2009;46(1):58–63. https://doi.org/10.1111/j.1600–079X.2008.00625.x
  3. Back K, Tan DX, Reiter RJ. Melatonin biosynthesis in plants: multiple pathways catalyze tryptophan to melatonin in the cytoplasm or chloroplasts. J Pineal Res. 2016;61(4):426–37. https://doi.org/10.1016/j.postharvbio.2021.111571
  4. Bal E. Effect of melatonin treatments on biochemical quality and postharvest life of nectarines. J Food Meas Charact. 2021;15(1):288–95. https://doi.org/10.1007/s11694–020–00636–5
  5. Bal E. Physicochemical changes in s’anta Rosa'plum fruit treated with melatonin during cold storage. J Food Meas Charact. 2019;13:1713–20. https://doi.org/10.1007/s11694–019–00088–6
  6. Bantayehu M, Alemayehu M. Efficacy of postharvest technologies on ripening behavior and quality of banana varieties grown in Ethiopia. Int J Fruit Sci. 2020;20(1):59–75. https://doi.org/10.1080/15538362.2019.1583623
  7. Blankenship SM, Ellsworth DD, Powell RL. A ripening index for banana fruit based on starch content. Hort Techn. 1993;3(3):338–39. https://doi.org/10.21273/HORTTECH.3.3.338
  8. Cao S, Bian K, Shi L, Chung H–H, Chen W, Yang Z. Role of melatonin in cell–wall disassembly and chilling tolerance in cold–stored peach fruit. J Agric Food Chem. 2018;66(22):5663–70. https://doi.org/10.1021/acs.jafc.8b02055
  9. Cheng J, Zheng A, Li H, Huan C, Jiang T, Shen S. Effects of melatonin treatment on ethanol fermenation and ERF expression in kiwifruit cv. Bruno during postharvest. Scientia Horticulturae. 2022;293:110696. https://doi.org/10.1016/j.scienta.2021.110696
  10. Dwivany FM, Aprilyandi AN, Suendo V, Sukriandi N. Carrageenan edible coating application prolongs Cavendish banana shelf life. Int J Food Sci. 2020;2020(1):8861610. https://doi.org/10.1155/2020/8861610
  11. Fan S, Li Q, Feng S, Lei Q, Abbas F, Yao Y. Melatonin maintains fruit quality and reduces anthracnose in postharvest papaya via enhancement of antioxidants and inhibition of pathogen development. Antioxidants. 2022;11(5):804. https://doi.org/10.3390/antiox11050804
  12. Fan S, Xiong T, Lei Q, Tan Q, Cai J, Song Z. Melatonin treatment improves postharvest preservation and resistance of guava fruit (Psidium guajava L.). Foods. 2022;11(3):262. https://doi.org/10.3390/foods11030262
  13. Gao H, Zhang ZK, Chai HK, Cheng N, Yang Y, Wang DN. Melatonin treatment delays postharvest senescence and regulates reactive oxygen species metabolism in peach fruit. Postharvest Biol Technol. 2016;118:103–10. https://doi.org/10.1016/j.postharvbio.2016.03.006
  14. Gurjar P, Singh S, Verma A, Mishra M. Post–harvest melatonin application reduced browning in minimally processed lettuce (Lactuca sativa L.) during low temperature storage. J Hortic Sci. 2023;18(1):195–200. https://doi.org/10.24154/jhs.v18i1.2163
  15. Hiscox J, Israelstam G. A method for the extraction of chlorophyll from leaf tissue without maceration. Canad J Bot. 1979;57(12):1332–34. https://doi.org/10.1139/b79–163
  16. Miao HM, Ying LJ, Ping RJ. Effect of melatonin on ripening and senescence of postharvest kiwifruits. Food Sci. 2018;39(19):226–32. https://doi.org/10.7506/spkx1002-6630-201819035
  17. Hu W, Yang H, Tie W, Yan Y, Ding Z, Liu Y. Natural variation in banana varieties highlights the role of melatonin in postharvest ripening and quality. J Agric Food Chem. 2017;65(46):9987–94. https://pubs.acs.org/doi/abs/10.1021/acs.jafc.7b03354
  18. Inaba A, Liu X, Yokotani N, Yamane M, Lu W–J, Nakano R. Differential feedback regulation of ethylene biosynthesis in pulp and peel tissues of banana fruit. J Exp Bot. 2007;58(5):1047–57. https://doi.org/10.1093/jxb/erl265
  19. Jayarajan S, Sharma R. Impact of nitric oxide on shelf life and quality of nectarine (Prunus persica var. nucipersica). Acta Physiol Plant. 2018;40(12):207. https://doi.org/10.1007/s11738–018–2779–4
  20. Li S, Xu Y, Bi Y, Zhang B, Shen S, Jiang T. Melatonin treatment inhibits gray mold and induces disease resistance in cherry tomato fruit during postharvest. Postharvest Biol Technol. 2019;157:110962. https://doi.org/10.1016/j.postharvbio.2019.110962
  21. Liu C, Zheng H, Sheng K, Liu W, Zheng L. Effects of melatonin treatment on the postharvest quality of strawberry fruit. Postharvest Biol Technol. 2018;139:47–55. https://doi.org/10.1016/j.postharvbio.2018.01.016
  22. Liu J, Yang J, Zhang H, Cong L, Zhai R, Yang C. Melatonin inhibits ethylene synthesis via nitric oxide regulation to delay postharvest senescence in pears. J Agric Food Chem. 2019;67(8):2279–88. https://doi.org/10.1021/acs.jafc.8b06580
  23. Liu S, Huang H, Huber DJ, Pan Y, Shi X, Zhang Z. Delay of ripening and softening in 'Guifei'mango fruit by postharvest application of melatonin. Postharvest Biol Technol. 2020;163:111136. https://doi.org/10.1016/j.postharvbio.2020.111136
  24. Lorente–Mento JM, Guillén F, Castillo S, Martínez–Romero D, Valverde JM, Valero D. Melatonin treatment to pomegranate trees enhances fruit bioactive compounds and quality traits at harvest and during postharvest storage. Antioxidants. 2021;10(6):820. https://doi.org/10.3390/antiox10060820
  25. Lustriane C, Dwivany FM, Suendo V, Reza M. Effect of chitosan and chitosan–nanoparticles on post harvest quality of banana fruits. J Plant Biotechnol. 2018;45(1):36–44. https://doi.org/10.5010/JPB.2018.45.1.036
  26. Ma Q, Lin X, Wei Q, Yang X, Zhang Yn, Chen J. Melatonin treatment delays postharvest senescence and maintains the organoleptic quality of 'Newhall'navel orange (Citrus sinensis (L.) Osbeck) by inhibiting respiration and enhancing antioxidant capacity. Sci Hortic. 2021;286:110236. https://doi.org/10.1016/j.scienta.2021.110236
  27. Mahajan PV, Caleb OJ, Singh Z, Watkins CB, Geyer M. Postharvest treatments of fresh produce. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sci. 2014;372(2017):20130309. https://doi.org/10.1098/rsta.2013.0309
  28. Maqbool M, Ali A, Alderson PG, Zahid N, Siddiqui Y. Effect of a novel edible composite coating based on gum arabic and chitosan on biochemical and physiological responses of banana fruits during cold storage. J Agric Food Chem. 2011;59(10):5474–82. https://doi.org/10.1021/jf200623m
  29. Mubarok S, Rahman MI, Kamaluddin N, Solihin E. Impact of 1–methylcyclopropene combined with chitosan on postharvest quality of tropical banana 'Lady Finger'. Int J Food Prop. 2022;25(1):1171–85. https://doi.org/10.1080/10942912.2022.2074028
  30. Onik JC, Wai SC, Li A, Lin Q, Sun Q, Wang Z. Melatonin treatment reduces ethylene production and maintains fruit quality in apple during postharvest storage. Food Chem. 2021;337:127753. https://doi.org/10.1016/j.foodchem.2020.127753
  31. Ringer T, Blanke M. Non–invasive, real time in–situ techniques to determine the ripening stage of banana. J Food Meas Charact. 2021;15(5):4426–37. https://doi.org/10.1007/s11694–021–01009–2
  32. Sánchez M, Villalta R, Guzmán M. Combate de enfermedades poscosecha en banano (Musa AAA) con extractos metanólicos de hojas de Musa spp. con diferente grado de resistencia a sigatoka negra. CORBANA. 2021;66:15–28.
  33. Sati H, Bhardwaj R, Fawole OA, Pareek S. Postharvest melatonin application preserves quality and imparts chilling tolerance in peaches. J Food Biochem. 2023;2023(1):8126640. https://doi.org/10.1155/2023/8126640
  34. Shang F, Liu R, Wu W, Han Y, Fang X, Chen H. Effects of melatonin on the components, quality and antioxidant activities of blueberry fruits. Lebensmittel–Wissenschaft and Technologie (LWT). 2021;147:111582. https://doi.org/10.1016/j.lwt.2021.111582
  35. Sun Q, Zhang N, Wang J, Zhang H, Li D, Shi J. Melatonin promotes ripening and improves quality of tomato fruit during postharvest life. J Exp Bot. 2015;66(3):657–68. https://doi.org/10.1093/jxb/eru332
  36. Tijero V, Munoz P, Munne–Bosch S. Melatonin as an inhibitor of sweet cherries ripening in orchard trees. Plant Physiol Biochem. 2019;140:88–95. https://doi.org/10.1016/j.plaphy.2019.05.007
  37. Wang C, Yin LY, Shi XY, Xiao H, Kang K, Liu XY. Effect of cultivar, temperature and environmental conditions on the dynamic change of melatonin in mulberry fruit development and wine fermentation. J Food Sci. 2016;81(4):M958–M67. https://doi.org/10.1111/1750–3841.13263
  38. Wang Z, Pu H, Shan S, Zhang P, Li J, Song H. Melatonin enhanced chilling tolerance and alleviated peel browning of banana fruit under low temperature storage. Postharvest Biol Technol. 2021;179:111571. https://doi.org/10.1016/j.postharvbio.2021.111571
  39. Wei W, Yang Y–y, Wu C–j, Kuang J–f, Chen J–y, Lu W–j. MaMADS1–MaNAC083 transcriptional regulatory cascade regulates ethylene biosynthesis during banana fruit ripening. Hortic Res. 2023;10(10):uhad177. https://doi.org/10.1093/hr/uhad177
  40. Xiao Y–y, Chen J–y, Kuang J–f, Shan W, Xie H, Jiang Y–m. Banana ethylene response factors are involved in fruit ripening through their interactions with ethylene biosynthesis genes. J Exp Bot. 2013;64(8):2499–510. https://doi.org/10.1093/jxb/ert108
  41. Ze Y, Gao H, Li T, Yang B, Jiang Y. Insights into the roles of melatonin in maintaining quality and extending shelf life of postharvest fruits. Trends Food Sci Technol. 2021;109:569–78. https://doi.org/10.1016/j.tifs.2021.01.051
  42. Zhai R, Liu J, Liu F, Zhao Y, Liu L, Fang C. Melatonin limited ethylene production, softening and reduced physiology disorder in pear (Pyrus communis L.) fruit during senescence. Postharvest Biol Technol. 2018;139:38–46. https://doi.org/10.1016/j.postharvbio.2018.01.017
  43. Zhang H, Liu X, Chen T, Ji Y, Shi K, Wang L. Melatonin in apples and juice: Inhibition of browning and microorganism growth in apple juice. Molecules. 2018;23(3):521. https://doi.org/10.3390/molecules23030521
  44. Zhang H, Wang L, Shi K, Shan D, Zhu Y, Wang C. Apple tree flowering is mediated by low level of melatonin under the regulation of seasonal light signal. J Pineal Res. 2019;66(2):e12551. https://doi.org/10.1111/jpi.12551
  45. Zhang N, Zhang H–J, Sun Q–Q, Cao Y–Y, Li X, Zhao B. Proteomic analysis reveals a role of melatonin in promoting cucumber seed germination under high salinity by regulating energy production. Sci Rep. 2017;7(1):503. https://doi.org/10.1038/s41598–017–00566–1
  46. Zhang W, Cao J, Fan X, Jiang W. Applications of nitric oxide and melatonin in improving postharvest fruit quality and the separate and crosstalk biochemical mechanisms. Trends Food Sci Technol. 2020;99:531–41. https://doi.org/10.1016/j.tifs.2020.03.024
  47. Zhang Z, Wang T, Liu G, Hu M, Yun Z, Duan X. Inhibition of downy blight and enhancement of resistance in litchi fruit by postharvest application of melatonin. Food Chem. 2021;347:129009. https://doi.org/10.1016/j.foodchem.2021.129009
  48. Zhu L, Hu H, Luo S, Wu Z, Li P. Melatonin delaying senescence of postharvest Broccoli by regulating respiratory metabolism and antioxidant activity. Trans Chinese Soc Agric Engin. 2018;34(3):300–08.

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