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Research Articles

Vol. 13 No. sp2 (2026): Recent Advances in Agriculture

Application of machine learning tools for eco-strategising Fusarium wilt management of tomato caused by Fusarium oxysporum f. sp. lycopersici

DOI
https://doi.org/10.14719/pst.10010
Submitted
12 June 2025
Published
29-05-2026

Abstract

Fusarium wilt of tomato, caused by Fusarium oxysporum f. sp. lycopersici (FOL), severely impacts global tomato production. A field experiment evaluated 25 treatments, categorised as compost-based [Farmyard manure (FYM) + de-oiled sweet basil compost], biological control (FYM + bio-agents) and chemical fungicides under field conditions. A random forest machine learning (ML) model predicted treatment effects, validated through biological assays and statistical tests. The treatment combining FYM + de-oiled sweet basil compost (25 t/ha) with Trichoderma viride (5 kg/ha) and Pseudomonas fluorescens (5 kg/ha) demonstrated the highest efficacy. It reduced disease incidence while maintaining soil health. The model revealed antagonism between bio-agents and fungicides. This study highlights ML's potential in optimising integrated disease management (IDM), reducing resource use and expediting decision-making. The validated model provides a reliable decision-support tool for precise and sustainable management of Fusarium wilt in tomato.

References

  1. 1. Amari K, Huang C, Heinlein M. Potential impact of global warming on virus propagation in infected plants and agricultural productivity. Front Plant Sci. 2021;12:649768. https://doi.org/10.3389/fpls.2021.649768
  2. 2. Hedlund J, Longo SB, York R. Agriculture, pesticide use and economic development: a global examination (1990–2014). Rural Sociol. 2020;85(2):519–44. https://doi.org/10.1111/ruso.12303
  3. 3. Kasem SM, Baka Z, Metwally MAE, Ibrahim AA, Soliman M. Biocontrol agents of mycoflora to improve the physiological and genetic characteristics of maize plants. Egypt J Bot. 2024;64(3):298–317. https://doi.org/10.21608/ejbo.2024.294254.2868
  4. 4. Rajmohan KS, Chandrasekaran R, Varjani S. A review on occurrence of pesticides in environment and current technologies for their remediation and management. Indian J Microbiol. 2020;60(2):125–38. https://doi.org/10.1007/s12088-019-00841-x
  5. 5. Afrane G, Ntiamoah A. Use of pesticides in the cocoa industry and their impact on the environment and the food chain. Rijeka: InTech; 2011. https://doi.org/10.5772/17921
  6. 6. Singh M, Mishra M, Srivastava DK, Singh PK. Biological control of Fusarium wilt of tomato by arbuscular mycorrhizal fungi with intercropping. Plant Pathol Quarantine. 2020;10(1):1–9. https://doi.org/10.5943/ppq/10/1/1
  7. 7. Elansary HO, Yessoufou K, Shokralla S, Mahmoud EA, Skalicka-Woźniak K. Enhancing mint and basil oil composition and antibacterial activity using seaweed extracts. Ind Crops Prod. 2016;92:50–6. https://doi.org/10.1016/j.indcrop.2016.07.048
  8. 8. Cao Y, Chang ZZ, Ma Y, Yang H, Fu GQ. Control mechanism of Phytophthora blight of chilli pepper by anaerobically digested pig slurry. Chin J Eco-Agric. 2013;21(9):1119–26. https://doi.org/10.3724/sp.j.1011.2013.01119
  9. 9. Hussein AM, Aljarah NS. Seed treatment of okra with humic acid to control damping-off pathogen Fusarium solani. Bionatura. 2023;8:1–12. https://doi.org/10.21931/rb/css/2023.08.04.55
  10. 10. Afifi MMI, Ismail AM, Kamel SM, Essa TA. Humic substances: a powerful tool for controlling Fusarium wilt disease and improving cucumber growth. J Plant Pathol. 2017;99(1):61–7.
  11. 11. Farahat G, Salama NH, Elgamal IS, Ghazy NA, Elsharkawy MM. Impact of humic acid, vigamax and potassium phosphate on control of Cercospora leaf spot disease. Arch Phytopathol Plant Prot. 2022;55(14):1661–85. https://doi.org/10.1080/03235408.2022.2111249
  12. 12. López-Berges MS, Capilla J, Turrà D, Schafferer L, Matthijs S, Jöchl C, et al. HapX-mediated iron homeostasis is essential for rhizosphere competence and virulence of Fusarium oxysporum. Plant Cell. 2012;24(9):3805–22. https://doi.org/10.1105/tpc.112.098624
  13. 13. Sahoo BR, Sahu KC, S NR. Incidence of Fusarium wilt of tomato incited by Fusarium oxysporum f. sp. lycopersici in Odisha, India. Ecol Environ Conserv. 2023;29(Suppl):504–7. https://doi.org/10.53550/eec.2023.v29i06s.079
  14. 14. Zhan N, Kuang MY, Li CY, Liu SW, Deng GM, Viljoen A, et al. First report of Fusarium wilt of Iholena banana (Musa spp.) caused by Fusarium oxysporum f. sp. cubense TR4 in China. Plant Dis. 2022;106(12):3204. https://doi.org/10.1094/pdis-11-21-2621-pdn
  15. 15. Burandt QC, Deising HB, Von Tiedemann A. Limitations of synthetic fungicides and risks of copper-based protection in Europe. Environ Toxicol Chem. 2024;43(1):19–30. https://doi.org/10.1002/etc.5766
  16. 16. Ritchie F, Sapelli L, Smith JA, Paveley ND, Lees AK, Bain RA, et al. Integrated disease management strategies for late blight (Phytophthora infestans) on potato. Pest Manag Sci. 2025. https://doi.org/10.1002/ps.8629
  17. 17. Waltz L, Katari S, Hong C, Anup A, Colbert J, Potlapally A, et al. Cyberinfrastructure for machine learning applications in agriculture. Front Artif Intell. 2025;7. https://doi.org/10.3389/frai.2024.1496066
  18. 18. Wardana TK, Arkeman Y, Priandana K, Kurniawan F. Plant health assessment using random forest for drone-based agriculture. J Resti. 2023;7(3):637–45. https://doi.org/10.29207/resti.v7i3.4959
  19. 19. Song W, Zhou L, Yang C, Cao X, Zhang L, Liu X. Tomato Fusarium wilt and its chemical control strategies. Crop Prot. 2004;23(3):243–7. https://doi.org/10.1016/j.cropro.2003.08.007
  20. 20. Kumar S, Meena NL, Rawal P, Trivedi A, Singh V, Panwar NL, et al. Bio-efficacy of biochar against Fusarium oxysporum f. sp. radicis-cucumerinum. J Adv Biol Biotechnol. 2024;27(11):271–8. https://doi.org/10.9734/jabb/2024/v27i111610
  21. 21. Awasthi K, Gupta K, Srivastava R. Synthesis, characterization and impact of carbon-based nanomaterials on gram (Cicer arietinum) growth and soil properties. Curr Agric Res J. 2025;12(3):1090–105. https://doi.org/10.12944/carj.12.3.05
  22. 22. AOAC International. Official method 2017.13: total phenolic content in extracts. In: Official methods of analysis of AOAC International. 22nd ed. Oxford: Oxford University Press; 2023. https://doi.org/10.1093/9780197610145.003.413
  23. 23. Savargiv M, Masoumi B, Keyvanpour MR. A new random forest algorithm based on learning automata. Comput Intell Neurosci. 2021;2021:5572781. https://doi.org/10.1155/2021/5572781
  24. 24. Comprehensive R Archive Network. Breiman and Cutler’s random forests for classification and regression (randomForest version 4.7-1.2) [Internet]. 2024 [cited 2026 Jan 18]. https://cran.r-project.org/package=randomForest
  25. 25. Panse VG, Sukhatme PV. Statistical methods for agricultural workers. 3rd ed. New Delhi: Indian Council of Agricultural Research; 1954.
  26. 26. Liaw A, Wiener M. Classification and regression by randomForest. R News. 2002;2(3):18–22.
  27. 27. Student. The probable error of a mean. Biometrika. 1908;6(1):1–25. https://doi.org/10.2307/2331554
  28. 28. Kohavi R. A study of cross-validation and bootstrap for accuracy estimation and model selection. In: Proceedings of the 14th International Joint Conference on Artificial Intelligence (IJCAI); 1995 Aug 20-25; Montreal, Canada. Vol 2. p. 1137–43.
  29. 29. Willmott CJ, Matsuura K. Advantages of the mean absolute error over the root mean square error in assessing model performance. Clim Res. 2005;30:79–82. https://doi.org/10.3354/cr030079
  30. 30. James G, Witten D, Hastie T, Tibshirani R. An introduction to statistical learning: with applications in R. 2nd ed. New York: Springer; 2021. https://doi.org/10.1007/978-1-0716-1418-1
  31. 31. Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet. 1986;327(8476):307–10. https://doi.org/10.1016/S0140-6736(86)90837-8
  32. 32. Bouchtaoui EM, Haouas A, Dababat AA, Lahlali R, Benali A, Fahr M, et al. Mechanisms of compost-mediated suppression of plant pathogens. Appl Soil Ecol. 2024;203:105644. https://doi.org/10.1016/j.apsoil.2024.105644
  33. 33. Romano R, De Luca L, Aiello A, Pagano R, Di Pierro P, Pizzolongo F, et al. Basil (Ocimum basilicum) leaves as a source of bioactive compounds. Foods. 2022;11(20):3212. https://doi.org/10.3390/foods11203212
  34. 34. Yaldiz G, Camlica M, Ozen F. Essential oil composition of sweet basil (Ocimum basilicum) grown with organic fertilization. J Sci Food Agric. 2019;99(4):2005–13. https://doi.org/10.1002/jsfa.9468
  35. 35. Liu X, Shi Y, Kong L, Tong L, Cao H, Zhou H, et al. Long-term application of biocompost altered soil microbial diversity. Microorganisms. 2022;10(2):462. https://doi.org/10.3390/microorganisms10020462
  36. 36. Vicente I, Baroncelli R, Hermosa R, Monte E, Vannacci G, Sarrocco S. Secondary metabolites in Trichoderma ecophysiology. Fungal Biol Rev. 2022;39:83–99. https://doi.org/10.1016/j.fbr.2021.12.004
  37. 37. Riera N, Davyt D, Durán R, Iraola G, Lemanceau P, Bajsa N. Antibiotic from Pseudomonas fluorescens with antifungal activity against Rhizoctonia solani. Front Microbiol. 2023;14. https://doi.org/10.3389/fmicb.2023.1286926
  38. 38. Kumar S, Dhar S, Barthakur S, Rajawat MVS, Kochewad SA, Kumar S, et al. Farmyard manure modulates soil biological activity and wheat yield. Front Environ Sci. 2021;9. https://doi.org/10.3389/fenvs.2021.764489
  39. 39. Bolo P, Mucheru-Muna MW, Mwirichia RK, Kinyua M, Ayaga G, Kihara J. Farmyard manure influences zinc-solubilizing microbes in ferralsol. Agriculture. 2023;13(12):2217. https://doi.org/10.3390/agriculture13122217
  40. 40. Sheoran S, Prakash D, Yadav PK, Gupta RK, Al-Ansari N, El-Hendawy S, et al. Long-term FYM and fertilizer N effects on soil fertility. Sci Rep. 2024;14:72076. https://doi.org/10.1038/s41598-024-72076-w
  41. 41. Rathor P, Upadhyay P, Ullah A, Gorim LY, Thilakarathna MS. Humic acid improves wheat growth via hormone biosynthesis. AoB Plants. 2024;16(2):plae018. https://doi.org/10.1093/aobpla/plae018
  42. 42. Zhang C, Zhou T, Zhu L, Juhasz A, Du Z, Li B, et al. Soil microbial response to pyraclostrobin exposure. Environ Pollut. 2019;255:113164. https://doi.org/10.1016/j.envpol.2019.113164
  43. 43. Schuster M, Kilaru S, Steinberg G. Azoles activate programmed cell death in crop pathogenic fungi. Nat Commun. 2024;15:48157. https://doi.org/10.1038/s41467-024-48157-9
  44. 44. Samavat S, Samavat S, Wyka SA. Humic and fulvic acids against boxwood blight caused by Calonectria pseudonaviculata. For Pathol. 2020;50(2):e12583. https://doi.org/10.1111/efp.12583
  45. 45. LaMondia JA. Fungicide efficacy against Calonectria pseudonaviculata. Plant Dis. 2014;98(1):99–102. https://doi.org/10.1094/pdis-04-13-0373-re
  46. 46. Ali H, Shehzadi N, Zaheer M, Seleiman M, Aldhuwaib K, Khan WD, et al. Salicylic acid and farmyard manure alleviate cadmium stress in maize (Zea mays). Plants. 2023;12(17):3115. https://doi.org/10.3390/plants12173115
  47. 47. Chen Q, Song Y, An Y, Lu Y, Zhong G. Soil microorganisms and crop nutrition enhancement. Diversity. 2024;16(12):734. https://doi.org/10.3390/d16120734
  48. 48. Bar-Tal A, Ganmore-Neumann R, Ben-Hayyim G. Root architecture effects on nutrient uptake. In: Altman A, editor. Plant biotechnology and in vitro biology in the 21st century. Dordrecht: Springer; 1997. p. 39–45. https://doi.org/10.1007/978-1-4615-5403-5_5
  49. 49. Lombardi M, De Gara L, Loreto F. Determinants of root system architecture for stress resilience. Physiol Plant. 2021;172(4):2090–7. https://doi.org/10.1111/ppl.13439
  50. 50. Galindo-Castañeda T, Hartmann M, Lynch JP. Root architecture structures rhizosphere microbial associations. J Exp Bot. 2024;75(2):594–604. https://doi.org/10.1093/jxb/erad421
  51. 51. Postma JA, Lynch JP. Complementarity in root architecture for nutrient uptake. Ann Bot. 2012;110(2):521–34. https://doi.org/10.1093/aob/mcs082
  52. 52. Ozliman S, Yaldiz G, Camlica M, Ozsoy N. Essential oil components of Anethum graveolens under organic and inorganic systems. Chem Biol Technol Agric. 2021;8:24. https://doi.org/10.1186/s40538-021-00224-9
  53. 53. Krey KL, Nabity PD, Blubaugh CK, Fu Z, Van Leuven JT, Reganold JP, et al. Organic farming sharpens plant defense. Front Sustain Food Syst. 2020;4. https://doi.org/10.3389/fsufs.2020.00097
  54. 54. Khanday AH, Badroo IA, Wagay NA, Rafiq S. Role of phenolic compounds in plant disease resistance. In: Gupta BK, editor. Phenolic compounds in plants. Singapore: Springer; 2024. p. 455–79. https://doi.org/10.1007/978-981-99-3334-1_19
  55. 55. Ayankojo IT, Morgan KT, Kadyampakeni DM, Liu GD. Nitrogen and irrigation effects on tomato growth. HortScience. 2020;55(11):1744–55. https://doi.org/10.21273/hortsci15177-20
  56. 56. Liu T, Cheng Z, Meng H, Ahmad I, Zhao H. Tomato–garlic intercropping under organic cultivation. Sci Hortic. 2014;170:159–68. https://doi.org/10.1016/j.scienta.2014.02.039
  57. 57. Su J, Wang Y, Bai M, Peng T, Li H, Xu HJ, et al. Soil microbiome boosts monoterpene accumulation in Citrus reticulata ‘Chachi’. Microbiome. 2023;11:150. https://doi.org/10.1186/s40168-023-01504-2
  58. 58. Aasfar A, Bargaz A, Yaakoubi K, Hilali A, Bennis I, Zeroual Y, et al. Nitrogen-fixing Azotobacter as soil biological enhancers. Front Microbiol. 2021;12. https://doi.org/10.3389/fmicb.2021.628379
  59. 59. Singh M, Chauhan A, Singh PK. Suppression of Fusarium wilt in tomato via Rhizophagus intraradices and Trichoderma viride. Vegetos. 2025;38:1515–22. https://doi.org/10.1007/s42535-024-00935-y
  60. 60. Ajmal MS, Rashid I. In vitro management of early blight of tomato. Phytopathogenomics Dis Control. 2023;1(1):1–6. https://doi.org/10.22194/pdc/2.102
  61. 61. Harnly ME, Bradman A, Nishioka M, McKone TE, Smith D, McLaughlin R, et al. Pesticides in household dust from agricultural areas. Environ Sci Technol. 2009;43(23):8767–74. https://doi.org/10.1021/es9020958
  62. 62. Moo-Muñoz AJ, Azorín-Vega EP, Ramírez-Durán N, Moreno-Pérez PA. Cytotoxicity of fungicides in human fibroblasts. J Environ Sci Health B. 2021;56(10):877–83. https://doi.org/10.1080/03601234.2021.1972721
  63. 63. Tyagi A, Tamang TL, Kashtoh H, Mir RA, Mir ZA, Manzoor S, et al. Biocontrol agents as sustainable crop disease management tools. Horticulturae. 2024;10(8):805. https://doi.org/10.3390/horticulturae10080805
  64. 64. Islam T, Danishuddin N, Tamanna NT, Matin MN, Barai HR, Haque MA. Fungicide resistance mechanisms and sustainable solutions. Plants. 2024;13(19):2737. https://doi.org/10.3390/plants13192737
  65. 65. Ghorbani M, Konvalina P, Neugschwandtner RW, Kopecký M, Amirahmadi E, Bucur D, et al. Biochar interactions with nitrogen fertilizers. Agronomy. 2022;12(9):2106. https://doi.org/10.3390/agronomy12092106
  66. 66. Ayyandurai M, Therademani M, Raja IY, Balakumbahan R, Kumari SMP, Pushpam AK, et al. Trichoderma spp. in sustainable disease management. J Anim Plant Sci. 2024;34(2):304–17. https://doi.org/10.36899/japs.2024.2.0718
  67. 67. Maurya S, Ntakirutimana R, Rana BDM, Srivastava DK. Trichoderma secondary metabolites in plant disease management. Biopestic Int. 2024;20(1):21. https://doi.org/10.59467/bi.2024.20.21
  68. 68. Borkakati RN, Deka MK, Borah BK, Pathak S, Paswan RP, Rahman N, et al. Ecology and management of papaya mealybug Paracoccus marginatus. J Adv Biol Biotechnol. 2024;27(10):1104–12. https://doi.org/10.9734/jabb/2024/v27i101531
  69. 69. Pandao MR, Thakare AA, Choudhari RJ, Navghare NR, Sirsat DD, Rathod SR. Soil health and nutrient management. Int J Plant Soil Sci. 2024;36(5):873–83. https://doi.org/10.9734/ijpss/2024/v36i54583
  70. 70. Vasilchenko AV, Poshvina DV, Semenov MV, Timofeev VN, Iashnikov AV, Stepanov AA, et al. Triazole–strobilurin mixtures affect soil microbes. Plants. 2023;12(3):660. https://doi.org/10.3390/plants12030660
  71. 71. Perrony PEP, Guimarães RA, Reis LO, Gomes LB, Da Silva LJ, De Albuquerque Correa CM, et al. Selectivity of foliar treatments against Fusarium verticillioides. J Phytopathol. 2023;171(11–12):656–72. https://doi.org/10.1111/jph.13226
  72. 72. Lupwayi NZ, Turkington TK, Tidemann BD, Kubota H, Polo RO. Crop rotation and fungicide effects on wheat rhizosphere microbes. Front Agron. 2024;6. https://doi.org/10.3389/fagro.2024.1455448
  73. 73. Ons L, Bylemans D, Thevissen K, Cammue BPA. Combining biocontrol agents with fungicides for integrated control. Microorganisms. 2020;8(12):1930. https://doi.org/10.3390/microorganisms8121930
  74. 74. Aghdam R, Tang X, Shan S, Lankau R, Solís-Lemus C. Human limits in machine learning for potato yield prediction. BMC Bioinformatics. 2024;25:5977. https://doi.org/10.1186/s12859-024-05977-2
  75. 75. Wang Z, Xiao S, Li J, Wang J. Learning-based agricultural management under climate variability. arXiv [Preprint]. 2024 [cited 2026 Jan 18]. https://arxiv.org/abs/2401.01273
  76. 76. Yousaf A, Kayvanfar V, Mazzoni A, Elomri A. AI-based decision support systems in smart agriculture. Front Sustain Food Syst. 2023;6. https://doi.org/10.3389/fsufs.2022.1053921
  77. 77. Hall RL, De Santana FB, Grunsky EC, Browne MA, Lowe V, Fitzsimons M, et al. Machine learning for predicting plant-available phosphorus. J Soils Sediments. 2023. https://doi.org/10.1007/s11368-023-03648-y

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