Effects of gamma radiation on quantitative traits and genetic variation of three successive generations of cowpea (Vigna unguiculata (L.) Walp.)
DOI:
https://doi.org/10.14719/pst.2021.8.3.1054Keywords:
Gamma irradiation, genetic variation, mutat, Yield, SCoT marker, SCoTAbstract
An annual pulse crop cowpea (Vigna unguiculata (L.) Walp.), commonly named southern pea, is a nourishing constituent for the human diet and fodder. Gamma rays are a potent mutagenic agent to stimulate genetic variation with better characteristics, improving the yield relating traits in crops. Hence, the present study focused on exploring genetic variation between three generations in the mutant populations of cowpea through SCoT markers. The mutant populations of three successive generations, M1, M2 and M3, were induced by different doses [200, 400, 600, 800, 1000 and 1200 Gray (Gy)] gamma irradiation. The results depict that the quantitative characters were reduced by increasing the dosage of gamma irradiation in the M1 generation. In contrast, the second and third generation of plants showed a significant increase in yield and yield contributing traits than control and the maximum increase was noticed at 200 Gy and 400 Gy. Days to first flowering was delayed in irradiated plants than control of M1 generation. In contrast, in consecutive generations (M2 and M3), the early first flowering was noticed at 400 Gy and late flowering was observed at 800 Gy compared respectively to control and other doses. Seed yield per plant mean value was increased at 200 Gy in both generations (M2 and M3); it may produce new genotypes to desirable traits such as yield and quality. SCoT markers were used to explore genetic variation at the genomic level of mutant populations and screened with eight primers. Among them, seven primers showed amplification of 222 bands, in which 133 bands showed polymorphism. The polymorphic bands varied from 3.03–96.07%. The genetic variation, such as the number of different alleles (Na), effective number of alleles (Ne), Shannon’s information index (I), expected heterozygosity (He) and unbiased expected heterozygosity (uHe) showed an average value of 1.352 ± 0.092, 1.278 ± 0.027, 0.293 ± 0.023, 0.184 ± 0.016, and 0.194 ± 0.016, respectively. AMOVA depicted significant genetic variation between all generations and indicated a total of 95% within populations and 5% among population variation by the marker used. The present investigations prominently showed that the variations induced by gamma irradiation were inherited from successive generations of the improvement in cowpea quantitative traits. This investigation gives acceptable proof that the SCoT markers are a valuable tool to identify the genetic variation among the three generations of cowpea.
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Elhardallou SB, Khali II, Gobouri AA, Abdel-Hafez SH. Amino acid composition of cowpea (Vigna ungiculata (L.) Walp) flour and its protein isolates. Food Nut Sci. 2015;(6):790-97. http://dx.doi.org/10.4236/fns.2015.69082
Magloire N. The genetic, morphological and physiological evaluation of African cowpea genotypes. Thesis, University of Free State, 2005.
Ajayi AT, Adesoye AI. Cluster analysis technique for assessing variability in cowpea (Vigna unguiculata (L.) Walp) accessions from Nigeria. Rata Povrt. 2013;50(2):1-7. https://doi.org/10.5937/ratpov50-4069
Wongpiyasatid A, Chotechuen S, Hormchan P, Ngampongsais S, Promcham W. Induced mutations in mung bean breeding regional yield trail of mung bean mutant lines. Kaset J. 2000;34:443-49.
Dhanavel D, Pavadai P, Mullainathan L, Mohana D, Raju G, Girija M, Thilagavathi M. 2008. Effectiveness and efficiency of chemical mutagens in cowpea (Vigna unguiculata L. Walp.). Afr J Biotech. 2008;7:4116-17. ISSN 1684–5315
De Ronde JA, Spreeth MH. Development and evaluation of drought resistant mutant germ-plasm of Vigna unguiculata. Agric Res Council (ARC). 2007;33:381-86.
Gnanamurthy S, Dhanavel D, Girij M, Pavadai P, Bharathi T. Effect of chemical mutagenesis on quantitative traits of maise (Zea mays L.). Inter J Res Bot. 2012;2(4):34-36.
Ahloowalia BS, Maluszynki M, Nichterlein K. Global impact of mutation derived varieties. Euphytica. 2004;135:187-04. https://doi.org/10.1023/B:EUPH.0000014914.85465.4f
Khan MH, Tyagi SD. Studies on effectiveness and efficiency of gamma rays, EMS and their combination in soybean (Glycine max (L.) Merrill). J Plant Breed Crop Sci. 2010;(2):55-58.
Thilagavathi C, Mullainathan L. Isolation of macro mutants and mutagenic effectiveness efficiency in black gram (Vigna mungo (L.) Hepper). Global J Mol Sci. 2009;4(2):76-79.
Yasmin K, Arulbalachandran D, Soundarya V, Vanmathi S. Effects of gamma radiation (?) on biochemical and antioxidant properties in black gram (Vigna mungo L. Hepper). Inter J Radia Biol. 2019;95(8):1135-43. https://doi.org/10.1080/09553002.2019.1589022
Yasmin K, Arulbalachandran D, Dilipan E, Vanmathi S. Characterization of 60CO ?-ray induced pod trait of blackgram-A promising yield mutants. Inter J Rad Biol. 2020;96(7):929-36. https://doi.org/10.1080/09553002.2020.1748738
Bolbhat SN, Dhumal KN. Induced macromutations in horsegram (Macrotyloma uniflorum (Lam.) Verdc). Legume Res. 2009;32(4):278-81.
Manjaya JG. Genetic improvement of soybean variety VLS-2 through induced mutations. In induced plant mutations in genomics era. Food and Agriculture Organization of the United State, 2009;106-10.
International atomic energy agency /mutant variety database (IAEA/MVD). 2019. https://mvd.iaea.org 6th September, 2019.
Song HS, Kang SY. Application of natural variation and induced mutation in breeding and functional genomics: Papers for International Symposium; Current status and future of plant mutation breeding. Korean J Breed Sci. 2003;35(1):24-34.
Kharkwal MC, Pandey PN, Pawar SE. Mutation breeding for crop improvement. In: Plant breeding – Mendelian to molecular approaches. Jain HK, Kharkwal MC, (eds), Narosa Publishing House, New Delhi, India, 2004;601- 45. https://doi.org/10.1007/978-94-007-1040-5_26
Nagatomi S, Degi K. Mutation breeding of chrysanthemum by gamma field irradiation and in vitro culture. In: Shu QY (ed.) Induced plant mutations in the genomics era. Food and Agriculture Organization of the United Nations, Rome, 2004;258-61.
Mehlo L, Mbambo Z, Bado S, Lin J, Moagi S, Buthelezi S, Stoychev S, Chikwamba R. Induced protein polymorphisms and nutritional quality of gamma irradiation mutants of sorghum. Mut Res. 2013;749(1-2):66-72. https://doi.org/10.1016/j.mrfmmm.2013.05.002
Dhakshnamoorthy D, Selvaraj R, Chidambaram ALA. Induced mutagenesis in Jatropha curcas L. using gamma rays and detection of DNA polymorphism through RAPD marker. CR Biol. 2011;334(1):24-30. https://doi.org/10.1016/j.crvi.2010.11.004
Kehinder OB, Myers GO, Fawole I. Analysis of genetic linkage in the cowpea Vigna unguiculata. Pak J Trop Agric Sci. 1997;20:75-82.
Adekola O, Oluleye F. Induction of genetic variation in cowpea (Vigna unguiculata (L.) Walp.) by gamma irradiation. Asian J Plant Sci. 2007;6:869-73. https://doi.org/10.3923/ajps.2007.869.873
Silveria G, Moliterno E, Ribeiro G, Costa PMA, Woyann LG, Tessmann EW, Oliveira EAC, Cruz CD. Increasing genetic variability in black oats using gamma irradiation. Genet Mol Res. 2014;13:10332-40. https://doi.org/10.4238/2014.December.4.28
Collard BCY, Mackill DJ. Start codon targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Mol Biol Rep. 2009;27:86-93. https://doi.org/10.1007/s11105-008-0060-5
Mulpuri S, Muddanuru T, Francis G. Start codon targeted (SCoT) polymorphism in toxic and non-toxic accessions of Jatropha curcas L. and development of a co-dominant SCAR marker. Plant Sci. 2013;207:117-27. https://doi.org/10.1016/j.plantsci.2013.02.013
Xiong F, Zhong R, Han Z, Jiang J, He L, Zhuang W et al. Start codon targeted polymorphism for evaluation of functional genetic variation and relationships in cultivated peanut (Arachis hypogaea L.) genotypes. Mol Biol Rep. 2011;38:3487-94. https://doi.org/10.1007/s11033-010-0459-6
Nei M, Li WH. Mathematical model for studying genetic variation in terms of restriction endonucleases. Pro Nat Acad Sci. 1979;76:5269-73. https://doi.org/10.1073/pnas.76.10.5269
Rohlf FJ. NTSYS-pc Version. 2.02i Numerical Taxonomy and Multivariate Analysis System. Applied Biostatistics Inc., Exeter Software, Setauket, New York. 1997.
Page RD. TREEVIEW: an application to display phylogenetic trees on personal computers. Com Appl Biosci. 1996;12:357-58. https://doi.org/10.1093/bioinformatics/12.4.357
Peakall R, Smouse PE. GENALEX 6: genetic analysis in excel. Population genetic software for teaching and research. Mol Ecol. 2006;6:288-95. https://doi.org/10.1111/j.1471-8286.2005.01155.x
Sangsiri C, Sorajjapinum W, Srinivesc P. Gamma irradiation induced mutations in mungbean. Sci Asia. 2005;31:251-55. https://doi.org/10.2306/scienceasia1513-1874.2005.31.251
Gunasekaran A, Pavadai P. Effect of gamma rays on germination, morphology, yield and biochemical studies in groundnut (Arachis hypogaea L.). World Sci Newslett. 2005;23:13-23.
Wani A, Anis M. Gamma ray and EMS induced bold seeded high yielding mutants in chickpea (Cicer arietinum L.). Turkish J Bio. 2008;32:1-5.
Girija M, Gnanamurthy S, Dhanavel D. Genetic diversity analysis of cowpea mutant (Vigna unguiculata (L.) Walp.) as revealed by RAPD marker. Inter J Adv Res. 2013;1:139-47.
Desai AS, Rao S. Effect of gamma radiation on germination and physiological aspects of pigeon pea (Cajanus cajan L. Mill) seedlings. IMPACT: Intern J Res Appl Nat Soc Sci. 2014;(2):47-52.
Mensah JK, Akomeah PA. Mutagenic effect of hydroxylamine and streptomycin on the growth and seed yield of cowpea (Vigna unguiculata (L.) Walp). Legume Res. 1997;15:39-44.
Rizwana Banu M, Kalamani A, Ashok S, Makesh N. Effect of mutagenic treatments on quantitative characters in M1 generation of cowpea (Vigna unguiculata (L.) Walp). Adv Plant Sci. 2005;18(11):505-10.
Girija M, Dhanavel D. Effect of gamma rays on quantitative traits of cowpea in M1 generation. Inter J Res Biol Sci. 2013;3(2):84-87.
Thilagavathi C, Mullainathan L. Influence of physical and chemical mutagens on quantitative characters of (Vigna mungo (L.) Hepper). Inter Multidis Res J. 2011;1(1):6-8.
Yasmin K, Arulbalachandran D. Effect of gamma rays on morphological and quantitative traits of black gram (Vigna mungo (L.) Hepper) in M1 generation. Inter J Curr Tre Res. 2016;4(2):5-12.
Justin Mudibu KC, Kabwe, Nkongolo, Adrien Kalonji-Mbuyi and Roger V. kizungu. Effect of gamma irradiation on morpho-agronomic characteristics of soybeans (Glycine max L.). Ame J Plant Sci. 2012;3:331-37. https://doi.org/10.4236/ajps.2012.33039
Bolbhat Sadashiv N, Bhoge Vikram D, Dhumal Konddiram N. Effect of mutagens on seed germination, plant survival and quantitative characters of horse gram (Macrotyloma uniflorum (Lam.) Verdc). Inter J Life Sci Pharma Res. 2012;2(4):129-36.
Khursheed S, Laskar RA, Raina A, Amin R, Khan S.
Comparative analysis of cytological abnormalities induced in
Vicia faba L. genotypes using physical and chemical mutagenesis. Chromosome Sci. 2015;18:3-7.
Pitirmovae MA. Effect of gamma rays and mutagens on barley seeds. Fiziol Res. 1979;6:127-31.
Arulbalachandran D, Mullainathan L, Velu S, Thilagavathi C. Genetic variability, heritability and genetic advance of quantitative traits in black gram by effects of mutation in field trail. Afr J Biotech. 2010;9(19):2731-35.
Rao SK. Gamma ray induced morphological and physical variations in Cicer arietinum L. Ind J Bot. 1988;11(1):29-32.
Khan M, Qureshi AS, Ibrahim M. Genetic variability induced by gamma irradiation and its modulation with gibberellic acid in M2 generation of chickpea (Cicer arietinum L.). Pak J Bot. 2005;7:285-92.
Lavanya GR, Yadav L, Suresg Babu G, Jyotipaul P. Sodium azide mutagenic effect on biological parameters and induced genetic variability in mung bean. J Food Leg. 2011;42(1):46-49.
Shinde MD. Assessment of variability in M3 lines of pigeonpea (Cajanus cajan (L.) Millsp.). M.Sc Thesis, Mahatma Phule Krishi Vidyapeeth, Rahuri, India. 2007.
Sagade SV. Genetic improvement of urdbean (Vigna mungo (L.) Hepper) through mutation breeding. Ph.D. Thesis, University of Pune, Pune (MS), India. 2008.
Patil MT. Genetic improvement of cowpea for agronomic traits, through mutation breeding. Ph. D. Thesis, University of Pune. 2009.
Waghmare VN, Mehra RB. Induced chlorophyll mutations, mutagenic effectiveness and efficiency in Lathyrus sativus L. Indian J Genet Plant Breed. 2001;61:53-56.
Odeigah PGC, Osanyinpeju AO, Myers GO. Induced mutations in cowpea (Vigna unguiculata (L.) Walp.). Rev De Bio Trop. 1998;46(3):579-86. https://doi.org/10.15517/rbt.v46i3.20117
Singh G, Sarean PK, Saharan RP, Singh A. Induced variability in mung bean (Vigna radiata (L.) Wilczek). Indian J Genet. 2001;61:281-82.
Deepalakshmi AJ, Anandakumar CR. Efficiency and effectiveness of physical and chemical mutagens in urdbean (Vigna mungo (L.) Hepper). Madras Agric J. 2003;90 (7-9):485-89.
Wani MR, Khan S, Parveen K. Induced variation for quantitative traits in mungbean. Indian J Appl Pure Bio. 2005;20:55-58.
Tah PR. Induced macro mutation in mungbean (Vigna radiata (L.) Wilczek). Inter J Bot. 2006;2:219-28. https://doi.org/10.3923/ijb.2006.219.228
Arulbalachandran D, Mullainathan L. Changes on quantitative traits of black gram (Vigna mungo (L.) Hepper) induced by EMS in M2 generation. J Phytol. 2009;1(4):230-35.
Singh SP, Singh NK, Singh RP, Prasad JP. Mutagenic effect of gamma rays and EMSon nodulation, yield and yield traits on lentil. Indian J Pulses Res. 2006;19:53-55.
Ravichandran V, Jayakumar. Effects of mutagens on quantitative characters in M2 and M3 generation of sesame (Sesamum indicum L.). Intern Lett Nat Sci. 2015;(42):76-82. https://doi.org/10.18052/www.scipress.com/ILNS.42.76.
Girhe S, Choudhary AD. Induced morphological mutants in Lathyrus sativus. J Cytol Genet. 2002;3:1-6.
Govindaraj M, Vetriventhan M, Srinivasan M. Importance of genetic diversity assessment in crop plants and its recent advances: an overview of its analytical perspectives. Genet Res Inter-Hindwai Public Corpor. 2015;1-14. https://doi.org/10.1155/2015/431487.
Chahal GS, Gosal SS. Principles and procedures of plant breeding. Oxford: Alpha Science International Limited. 2002;399-412.
Khan S, Parveen K, Goyal S. Induced mutations in chickpea morphological mutants. Front Agric China. 2011;(5):35-39. https://doi.org/10.1007/s11703-011-1050-1
Clegg MT. Plant genetic diversity and the struggle to measure selection. J Here. 1997;88(1):1-7. https://doi.org/10.1093/oxfordjournals.jhered.a023048
Ezzat A, Adlyb M, El-Fikib A. Morphological, agronomical and molecular characterisation in irradiated cowpea (Vigna unguiculata (L.) Walp.) and detection by start codon target markers. J Rad Res Appl Sci. 2019;12(1):403-12. https://doi.org/10.1080/16878507.2019.1686578
Diouf D, Hilu KW. Microsatellites and RAPD markers to study genetic relationship among cowpea breeding lines and local varieties in Senegal. Genet Res Crop Evol. 2005;(52):1057-67. https://doi.org/10.1007/s10722-004-6107-z
El-Khateeb MA, Rawia AE, Heba AM, Ashor HA, Mabrouk RMS. Induction of mutation with gamma radiation in Helichrysum bracteatum L. and Identification of mutants by molecular markers. Middle East J Agric Res. 2017;6(2):282-93.
Yoko K, Aya M, Hiromi I, Takashi Y, Kukio S. Effect of gamma irradiation on cereal DNA investigated by pulsed field gel electrophoresis. Shok Shosha. 1996;31:8-15.
Hanafy RS, Akladious SA. Physiological and molecular studies on the effect of gamma radiation in fenugreek (Trigonella foenum-graecum L.) plants. J Genet Engi Biotechnol. 2018;(16):683-92. https://doi.org/10.1016/j.jgeb.2018.02.012
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