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Plant Science Today (PST)

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Antimicrobial potential of Rhus coriaria, Hibiscus sabdariffa, Syzygium aromaticum and Punica granatum plant extracts against Streptococcus pneumoniae: An in vitro study

DOI
https://doi.org/10.14719/pst.10552
Submitted
9 July 2025
Published
26-03-2026

Abstract

For a long time, traditional treatment has gained approbation of ancient people, elders and people under poverty. The antibiotic-resistant, Gram-positive, virulent bacterium Streptococcus pneumoniae is one of the most significant pathogens responsible for a wide range of nasopharyngeal infections. These infections are often persistent and difficult to treat due to the organism’s Gram-positive nature and multiple virulence factors, including a polysaccharide capsule that protects the bacterium from host defenses. In addition, S. pneumoniae rapidly develops mechanisms of antibiotic resistance, further complicating effective treatment. Finding a sustainable alternative treatment, particularly from plant sources, has gained traction due to the bacterium's increasing antibiotic resistance. Aqueous and alcoholic extracts of Rhus coriaria L., Hibiscus sabdariffa L., Syzygium aromaticum (L.) Merr. & L.M.Perry and Punica granatum L., were assayed on S. pneumoniae, which was diagnosed by biochemical tests. Sensitivity test was carried out in comparison with standard antibiotic disc. Cytotoxicity test on blood cells were also performed. The antimicrobial susceptibility assays showed that S. pneumoniae was moderately sensitive for R. coriaria, H. sabdariffa, S. aromaticum and P. granatum. These plant extracts can be suggested as a traditional treatment alternative to antibiotics.

References

  1. 1. Cillóniz C, Garcia-Vidal C, Ceccato A, Torres A. Antimicrobial resistance among Streptococcus pneumoniae. In: Antimicrobial resistance in the 21st century. Springer International Publishing; 2018. p. 13–38. https://doi.org/10.1007/978-3-319-78538-7_2
  2. 2. Tothpal A, Desobry K, Joshi S, Wyllie AL, Weinberger DM. Growth characteristics of pneumococcus vary with the chemical composition of the capsule and with environmental conditions. bioRxiv. 2019:416040. https://doi.org/10.1101/416040
  3. 3. Ahmed J, Malik F. Streptococcus pneumoniae. In: Encyclopedia of infection and immunity. Elsevier; 2022. p. 511–28. https://doi.org/10.1016/B978-0-12-818731-9.00033-1
  4. 4. Mitsi E, Nikolaou E, Goncalves A, Blizard A, Hill H, Farrar M, et al. RSV and rhinovirus increase pneumococcal carriage acquisition and density, whereas nasal inflammation is associated with bacterial shedding. Cell Host Microbe. 2024;32:1608–20.e4. https://doi.org/10.1016/j.chom.2024.07.024.
  5. 5. Kiran D, Aasritha B, Lakshmi B, Kumari J, Sujitha B, Swathi G. Pneumococcal vaccine: overall view. Int J Res Appl Sci Eng Technol. 2024;12:641–7. https://doi.org/10.22214/ijraset.2024.63970.
  6. 6. Al-Jumaili A, Dawood HN, Ikram D, Al-Jabban A. Pneumococcal disease: global disease prevention strategies with a focus on the challenges in Iraq. Int J Gen Med 2023:2095–110. https://doi.org/10.2147/IJGM.S409476
  7. 7. Abruzzo AR, Aggarwal SD, Sharp ME, Bee GCW, Weiser JN. Serotype-dependent effects on the dynamics of pneumococcal colonization and implications for transmission. mBio. 2022;13:e00158-22. https://doi.org/10.1128/mbio.00158-22
  8. 8. Sheppard CL, Manna S, Groves N, Litt DJ, Amin-Chowdhury Z, Bertran M, et al. PneumoKITy: A fast, flexible, specific and sensitive tool for Streptococcus pneumoniae serotype screening and mixed serotype detection from genome sequence data. Microb Genom 2022;8:904. https://doi.org/10.1099/mgen.0.000904
  9. 9. Jiang H, Meng Q, Liu X, Chen H, Zhu C, Chen Y. PspA diversity, serotype distribution and antimicrobial resistance of invasive pneumococcal isolates from paediatric patients in Shenzhen, China. Infect Drug Resist. 2021;14:49–58. https://doi.org/10.2147/IDR.S286187
  10. 10. Kurt B, Gul I. The significance of Rhus coriaria L. (sumac) plant in afforestation. 2024. p. 137–56. https://doi.org/10.69860/nobel.9786053359395.7.
  11. 11. Taheri S, Sohrabi Z, Bahari H, Mirmohammadali SN, Hashempur MH, Golafrouz H, et al. The effects of sumac consumption on blood pressure, glycemic indices and body composition in adults: a GRADE-assessed systematic review and dose-response meta-analysis. Diabetes Metab Syndr. 2024;18:103122. https://doi.org/10.1016/j.dsx.2024.103122
  12. 12. Ross IA. Hibiscus sabdariffa. In: Ross IA, editor. Medicinal plants of the world. Totowa (NJ): Humana Press; 2003. p. 267–75. https://doi.org/10.1007/978-1-59259-365-1_13
  13. 13. Dalziel JM. The useful plants of West Tropical Africa. London: The Crown Agents; 1973. p. 314–5.
  14. 14. Fullerton M, Khatiwada J, Johnson J, Davis S, Williams L. Determination of antimicrobial activity of sorrel (Hibiscus sabdariffa) on Escherichia coli O157:H7 isolated from food, veterinary, and clinical samples. J Med Food. 2011;14:950–956. https://doi.org/10.1089/jmf.2010.0200
  15. 15. Yasir M, Jamil N, Ali HMS, Hussain M, Naveed MA, Ahmed T, et al. Evaluation of antioxidant, cytoprotective and genoprotective potential of Syzygium aromaticum hydrolyzed extracts. 2022.
  16. 16. Ameen IA, Okab HF. Phyto-activity of Syzygium aromaticum extract against pathogenic bacteria isolated from chronic tonsillitis patients. Romanian J Infect Dis. 2024;127.
  17. 17. Mohan M, Mohanavarshaa CA, Priya D. Review of pharmacological and medicinal uses of Punica granatum. Cureus. 2024;16. https://doi.org/10.7759/cureus.71510
  18. 18. Shleghm MR, Mansoor AFA, Naeemah TC. Evaluation of the antibacterial effects of Punica granatum peels extracts against some pathogenic bacteria: an in vivo and in vitro study. Biomed Pharmacol J. 2024;17:2065–70. https://doi.org/10.13005/bpj/3008
  19. 19. Harborne JB. Methods of plant analysis. In: Phytochemical methods: a guide to modern techniques of plant analysis. 2nd ed. Springer; 1984. p. 1–36. https://doi.org/10.1007/978-94-009-5570-7_1
  20. 20. Vernet G, Saha S, Satzke C, Burgess DH, Alderson M, Maisonneuve JF, et al. Laboratory-based diagnosis of Pneumococcal pneumonia: state of the art and unmet needs. Clin Microbiol Infect. 2011;17:1–13. https://doi.org/10.1111/j.1469-0691.2011.03496.x
  21. 21. CLSI. Performance standards for antimicrobial susceptibility testing. CLSI M100. Clinical and Laboratory Standards Institute; 2025.
  22. 22. Al-Manhel AJ, Kareem Niamah A. Effect of aqueous and alcoholic plant extracts on inhibition of some types of microbes causing spoilage of food. J Nutr Food Sci. 2012;S5. https://doi.org/10.4172/2155-9600.s5-006
  23. 23. Parhusip AJN, Sitanggang AB. Antimicrobial activity of melinjo seed and peel extract (Gnetum gnemon) against selected pathogenic bacteria. Microbiol Indones. 2011;5:103–12. https://doi.org/10.5454/mi.5.3.2
  24. 24. Nair MG, Putnam AR, Mishra SK, Mulks MH, Taft WH, Keller JE, et al. Faeriefungin: a new broad-spectrum antibiotic from Streptomyces griseus var. autotrophicus. J Nat Prod. 1989;52:797–809. https://doi.org/10.1021/np50064a022
  25. 25. Snedecor GW, Cochran WG. Statistical methods. 8th ed. Ames: Iowa State University Press; 1989.
  26. 26. Fisher RA. Statistical methods for research workers. Vol 1. Edinburgh: Oliver and Boyd; 1925.
  27. 27. Kaur G, Chakraborty T. Scope of various phytoconstituents in the treatment of multidrug resistant bacteria: a review. Int J Adv Res. 2022;10:916–21. https://doi.org/10.21474/IJAR01/14970.
  28. 28. Subramani R, Narayanasamy M, Feussner KD. Plant-derived antimicrobials to fight against multi-drug-resistant human pathogens. 3 Biotech. 2017;7:1–15. https://doi.org/10.1007/s13205-017-0848-9
  29. 29. Joseph G, Koltai H, Ron EZ, Azzam N, Hazan H, Raskin I, et al. Rhus coriaria L. (sumac) leaves harbour robust antimicrobial activity. J Herb Med. 2023;41:100729. https://doi.org/10.1016/j.hermed.2023.100729
  30. 30. Alaamery S. Antibacterial and antibiofilm effect of sumac (Rhus coriaria L.) fruits extracts against some multidrug-resistant pathogenic bacteria. J Fac Med Baghdad. 2022;64. https://doi.org/10.32007/jfacmedbagdad.6431964.
  31. 31. Farooq S, Mehmood Z, Dixit A. Antimicrobial potentials of plant extracts against drug resistant bacteria. Univ J Phytochem Ayurv Heights. 2023;1:1–8. https://doi.org/10.51129/ujpah-2022-34-1(6).
  32. 32. Suganya T, Packiavathy IASV, Aseervatham GSB, Carmona A, Rashmi V, Mariappan S, et al. Tackling multiple-drug-resistant bacteria with conventional and complex phytochemicals. Front Cell Infect Microbiol. 2022;12:883839. https://doi.org/10.3389/fcimb.2022.883839
  33. 33. Javadian F, Sepehri Z, Amrayy M, Kiani Z, Shahreki Mujahid M, Shahi Z, et al. Evaluation of antibacterial activity of ethanolic extract of sour tea (Hibiscus sabdariffa) against antibiotic-resistant Klebsiella pneumoniae. Bimonthly J Sabzevar Univ Med Sci. 2015;22.
  34. 34. Oraibi SM. Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pyogenes and Proteus mirabilis virulence factors detected from hospitals in Nasiriyah, Iraq. Iran J Med Microbiol. 2024;18:209–13. https://doi.org/10.30699/ijmm.18.3.209
  35. 35. Hamid BTA, Hakim NAM, Abdelgader LMA, Mahjaf GM, Hammad KS, Altaher TA, et al. In vitro evaluation of antimicrobial activity of Syzygium aromaticum against bacteria isolated from different clinical specimens in Shendi Town, Sudan. 2024. https://doi.org/10.9734/sajrm/2024/v18i7375
  36. 36. Fatima S, Saba S, Hussain A, Aslam L, Naveed A. Antibacterial and anti-biofilm profiling of Syzygium aromaticum plant extracts against multidrug resistant human pathogens. Pak J Biochem Biotechnol. 2023;4:71–82. https://doi.org/10.52700/pjbb.v4i2.236
  37. 37. Salisu A, Iliyasu MY, Sahal MR, Titus I, Isma’il S, Umar RD, et al. Antimicrobial potential of Syzygium aromaticum (clove) extracts on multidrug-resistant uropathogenic bacteria. J Adv Med Pharm Sci. 2022:41–57. https://doi.org/10.9734/jamps/2022/v24i11588
  38. 38. Scaglione E, Sateriale D, Mantova G, Di Rosario M, Continisio L, Vitiello M, et al. Antimicrobial efficacy of Punica granatum Lythraceae peel extract against ESKAPE pathogens. Front Microbiol. 2024;15:1383027. https://doi.org/10.3389/fmicb.2024.1383027
  39. 39. de Lima LB, da Silva WAV, Dos Santos ECF, Machado JCB, Procópio TF, de Moura MC, et al. Evaluation of antioxidant, antibacterial and enhancement of antibiotic action by Punica granatum leaves crude extract and enriched fraction against multidrug-resistant bacteria. Chem Biodivers. 2021;18:e2100538. https://doi.org/10.1002/cbdv.202100538
  40. 40. Al Hawani IAA, Aldhaher AHS, Abdalzahra IM. A biological study on Quercus bark as antimicrobial agent. Ann Trop Med Public Health. 2020;23. https://doi.org/10.36295/ASRO.2020.231109
  41. 41. Chadha J, Gupta M, Nagpal N, Sharma M, Adarsh T, Joshi V, et al. Antibacterial potential of indigenous plant extracts against multidrug-resistant bacterial strains isolated from New Delhi region. GSC Biol Pharm Sci. 2021;14:185–96. https://doi.org/10.30574/gscbps.2021.14.2.0053
  42. 42. Kumar G, Rao B. Hemolytic activity of Indian medicinal plants towards human erythrocytes: an in vitro study. 2011.
  43. 43. Masoumian M, Zandi M. Antimicrobial activity of some medicinal plant extracts against multidrug resistant bacteria. Zahedan J Res Med Sci. 2017;19. https://doi.org/10.5812/zjrms.10080

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