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Plant Science Today (PST)

Research Articles

Vol. 13 No. sp5 (2026): Recent Advances in Agriculture

Understanding seed longevity in rice under variable temperature conditions

DOI
https://doi.org/10.14719/pst.11875
Submitted
20 September 2025
Published
27-08-2026

Abstract

A study involving fifty rice germplasm lines was conducted to investigate the effect of variable temperature and relative humidity (30 ± 2 °C for 90 days, followed by 50 ± 2 °C for two days at 60–70 % relative humidity (RH)) on seed longevity traits. The results confirmed a sharp decline in germination percentage (GP), speed of germination (SOG), seedling length (SL) and seed vigour index-I (SV-I) when compared to the quality of freshly harvested ones. The comparative analysis showed a significant positive correlation between GP and SOG (0.95) and between GP and SV-I (0.87). The fifty genotypes were grouped into three clusters, based on the similarities of behaviour under the treatment. The principal component analysis (PCA) also confirmed the grouping of the genotypes based on the seed quality traits under variable temperature treatments. The traits such as GP and SV-I were found highly affected on storage of seed at ambient temperature (30 ± 2 °C) followed by a sudden increase in temperature (50 ± 2 °C). Genotypes such as MDU-5, Manavari, Radhabati and PMK2 have showed least decrease in seed quality traits under variable temperature treatment. These germplasm lines could be used as suitable donor for development of climate resilient varieties which can ensure high-quality seed storage policies in future.

References

  1. 1. Goftishu M, Belete K. Susceptibility of sorghum varieties to the maize weevil Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae). Afr J Agric Res. 2014;9(31):2419–26. https://doi.org/10.5897/AJAR2014.8634
  2. 2. Shelar VR, Shaikh RS, Nikam AS. Soybean seed quality during storage: a review. Agric Rev. 2008;29(2):125–31.
  3. 3. Verdier J, Lalanne D, Pelletier S, Torres-Jerez I, Righetti K, Bandyopadhyay K, et al. A regulatory network-based approach dissects late maturation processes related to the acquisition of desiccation tolerance and longevity of Medicago truncatula seeds. Plant Physiol. 2013;163(2):757–74. https://doi.org/10.1104/pp.113.222380
  4. 4. Wilson DO. The storage of orthodox seeds. In: Seed quality. Boca Raton (FL): CRC Press; 2020. p. 173–207. https://doi.org/10.4324/9781003075226-6
  5. 5. Ali ML, Pathan MS, Zhang J, Bai G, Sarkarung S, Nguyen HT, et al. Mapping QTLs for root traits in a recombinant inbred population from two indica ecotypes in rice. Theor Appl Genet.2000;101(4):756–66. https://doi.org/10.1007/s001220051541
  6. 6. Amjad M, Anjum MA. Effect of relative humidity and ageing period on the quality of onion seed. Int J Agric Biol. 2002;4(2):291–6.
  7. 7. Fabrizius E, TeKrony DM, Egli DB, Rucker M. Evaluation of a viability model for predicting soybean seed germination during warehouse storage. Crop Sci. 1999;39:194–201. https://doi.org/10.2135/cropsci1999.0011183X003900010030x
  8. 8. Gebeyehu B. Review on: effect of seed storage period and storage environment on seed quality. Int J Appl Agric Sci.2020;6(6):185–90.
  9. 9. https://doi.org/10.11648/j.ijaas.20200606.14
  10. 10. Ramya MJ, Kulkarni GU, Deepthi R. Effect of packaging material, storage conditions and storage period on seed quality parameters of sesame (Sesamum indicum L.). Int J Pure Appl Biosci. 2018;6(5):309–13. https://doi.org/10.18782/2320-7051.6774
  11. 11. Jeevan Kumar SP, Rajendra Prasad S, Banerjee R, Thammineni C. Seed birth to death: dual functions of reactive oxygen species in seed physiology. Ann Bot. 2015;116:663–8. https://doi.org/10.1093/aob/mcv098
  12. 12. Groot SP, Surki AA, De Groot L, Kodde J, Pritchard HW. Desiccation tolerance of rattans: a herbarium-based comparative study. Ann Bot. 2017;119(4):655–64.
  13. 13. Hayashi M. Physiological studies on the relationship between levels of the endogenous germination inhibitors and the dormancy of rice seed. Bull Fac Agric Kagoshima Univ. 1986;36:1–43.
  14. 14. International Seed Testing Association (ISTA). International rules for seed testing. Bassersdorf (CH): International Seed Testing Association; 2020.
  15. 15. 14.Czabator FJ. Germination value: an index combining speed and completeness of pine seed germination. For Sci. 1962;8:386–95. https://doi.org/10.1093/forestscience/8.4.386
  16. 16. Abdul-Baki AA, Anderson JD. Vigour determination in soybean seed by multiple criteria. Crop Sci. 1973;13(6):630–3. https://doi.org/10.2135/cropsci1973.0011183X001300060013x
  17. 17. Gomez KA, Gomez AA. Statistical procedures for agricultural research. 2nd ed. New York (NY): Wiley; 1984.
  18. 18. Coolbear P, Francis A, Grierson D. The effect of low temperature pre-sowing treatment on the germination performance and membrane integrity of artificially aged tomato seeds. J Exp Bot. 1984;35:1609–17. https://doi.org/10.1093/jxb/35.11.1609
  19. 19. Doijode SD. Onion seed quality in relation to seed deterioration. Veg Sci. 1985;12:59–63.
  20. 20. Cantliffe DJ. Seed germination for transplants. HortTechnology. 1998;8(4):499–503. https://doi.org/10.21273/HORTTECH.8.4.499
  21. 21. Gupta R, Min CW, Cho JH, Jung JY, Jeon JS, Kim YJ, et al. Integrated- “omics” analysis highlights the role of brassinosteroid signaling and antioxidant machinery underlying improved rice seed longevity during artificial aging treatment. Plant Physiol Biochem. 2024;206:108308. https://doi.org/10.1016/j.plaphy.2023.108308
  22. 22. Nadarajan J, Walters C, Pritchard HW, Ballesteros D, Colville L. Seed longevity—the evolution of knowledge and a conceptual framework. Plants. 2023;12(3):471. https://doi.org/10.3390/plants12030471
  23. 23. Jiang L, Liu S, Hou M, Tang J, Chen L, Zhai H, Wan J. Analysis of QTLs for seed low temperature germinability and anoxia germinability in rice (Oryza sativa L.). Field Crops Res. 2006;98(1):68–75. https://doi.org/10.1016/j.fcr.2005.12.015
  24. 24. Wang B, Yang R, Ji Z, Zhang H, Zheng W, Zhang H, et al. Evaluation of biochemical and physiological changes in sweet corn seeds under natural aging and artificial accelerated aging. Agronomy. 2022;12(5):1028. https://doi.org/10.3390/agronomy12051028
  25. 25. Ballesteros D, Fanega-Sleziak N, Davies RM. Cryopreservation of seeds and seed embryos in orthodox-, intermediate- and recalcitrant-seeded species. In: Cryopreservation and freeze-drying protocols. New York (NY): Humana; 2021. p. 663–82. https://doi.org/10.1007/978-1-0716-0783-1_36
  26. 26. Sanghamitra P, Barik SR, Bastia R, Mohanty SP, Pandit E, Behera A, et al. Detection of genomic regions controlling the antioxidant enzymes, phenolic content and antioxidant activities in rice grain through association mapping. Plants. 2022;11(11):1463. https://doi.org/10.3390/plants11111463
  27. 27. Sahoo S, Sanghamitra P, Nanda N, Pawar S, Pandit E, Bastia R, et al. Association of molecular markers with physio-biochemical traits related to seed vigour in rice. Physiol Mol Biol Plants. 2020;26:1989–2003. https://doi.org/10.1007/s12298-020-00879-y
  28. 28. Pradhan SK, Pandit E, Pawar S, Naveenkumar R, Barik SR, Mohanty SP, et al. Linkage disequilibrium mapping for grain Fe and Zn enhancing QTLs useful for nutrient-dense rice breeding. BMC Plant Biol. 2020;20:57. https://doi.org/10.1186/s12870-020-2262-4
  29. 29. Bijanzadeh E, Naderi R, Nosrati K, Egan TP. Effects of accelerated ageing on germination and biochemistry of eight rice cultivars. J Plant Nutr. 2017;40(2):156–64. https://doi.org/10.1080/01904167.2016.1201502
  30. 30. Kapoor N, Arya A, Siddiqui MA, Kumar H, Amir A. Physiological and biochemical changes during seed deterioration in aged seeds of rice (Oryza sativa L.). Am J Plant Physiol. 2011;6(1):28–35. https://doi.org/10.3923/ajpp.2011.28.35

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