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Plant Science Today (PST)

Review Articles

Early Access

Antifungal peptides: A new approach to alternative postharvest fruit systems

DOI
https://doi.org/10.14719/pst.12314
Submitted
16 October 2025
Published
02-07-2026
Versions

Abstract

The search for sustainable, safe and effective alternatives for plant pathogen control has driven interest in the use of antimicrobial peptides. These short, bioactive molecules, characterised by high structural diversity and multiple mechanisms of action, have demonstrated efficacy against various plant pathogens and display a broad antimicrobial spectrum. This review offers a bibliometric analysis of the scientific literature related to the postharvest application of antifungal peptides, highlighting an increase in research from 2001–2025. It also examines the structural and functional classification, peptide synthesis and advantages of antimicrobial agents. Overall, antifungal peptides represent a promising tool for integrated postharvest disease management, adhering to the principles of food safety, sustainability and zero waste.

References

  1. 1. Gamage A, Gangahagedara R, Subasinghe S, Gamage J, Guruge C, Senaratne S, et al. Advancing sustainability: the impact of emerging technologies in agriculture. Curr Plant Biol. 2024;40:100420. https://doi.org/10.1016/j.cpb.2024.100420
  2. 2. Khani S, Seyedjavadi SS, Hosseini HM, Goudarzi M, Valadbeigi S, Khatami S, et al. Effects of the antifungal peptide Skh-AMP1 derived from Satureja khuzistanica on cell membrane permeability, ROS production and cell morphology of conidia and hyphae of Aspergillus fumigatus. Peptides. 2020;123:170195. https://doi.org/10.1016/j.peptides.2019.170195
  3. 3. Bucataru C, Ciobanasu C. Antimicrobial peptides: opportunities and challenges in overcoming resistance. Microbiol Res. 2024;286:127822. https://doi.org/10.1016/j.micres.2024.127822
  4. 4. Xu Z, Jiang X, Li Y, Ma X, Tang Y, Li H, et al. Antifungal activity of montmorillonite/peptide aptamer nanocomposite against Colletotrichum gloeosporioides on Stylosanthes. Int J Biol Macromol. 2022;217:282–90. https://doi.org/10.1016/j.ijbiomac.2022.07.034
  5. 5. Fan L, Wei Y, Chen Y, Jiang S, Xu F, Zhang C, et al. Epinecidin-1, a marine antifungal peptide, inhibits Botrytis cinerea and delays gray mold in postharvest peaches. Food Chem. 2023;403:134419. https://doi.org/10.1016/j.foodchem.2022.134419
  6. 6. Li J, Bai X, Zhu G, Liu S, Liu C, Wu M, et al. Sanxiapeptin is an ideal preservative with a dual effect of controlling green mold and inducing systemic defense in postharvest citrus. Food Chem. 2024;453:139669. https://doi.org/10.1016/j.foodchem.2024.139669
  7. 7. Li Z, Bi X, Dai Y, Ren R. Enhancing mango anthracnose control and quality maintenance through chitosan and iturin A coating. LWT. 2024;198:115955. https://doi.org/10.1016/j.lwt.2024.115955
  8. 8. Jia C, Mustafa H. A bibliometric analysis and review of nudge research using VOSviewer. Behavioral Sciences. 2022;13(1):19. https://doi.org/10.3390/bs13010019
  9. 9. Rayón-Díaz E, Hernández-Montiel LG, Sánchez-Burgos JA, Zamora-Gasga VM, González-Estrada RR, Gutiérrez-Martínez P. Natural compounds and derivates: alternative treatments to reduce post-harvest losses in fruits. AgriEngineering. 2024;6(2):1022–42. https://doi.org/10.3390/agriengineering6020059
  10. 10. Kawmudhi PAS, Chathurika S, Weerasinghe L. Applications of antimicrobial peptides in plant pest and disease control. Discover Plants. 2025;2(1):55. https://doi.org/10.1007/s44372-025-00134-2
  11. 11. Tang R, Tan H, Dai Y, Li L, Huang Y, Yao H, et al. Application of antimicrobial peptides in plant protection: making use of the overlooked merits. Front Plant Sci. 2023;14:1139539. https://doi.org/10.3389/fpls.2023.1139539
  12. 12. Parisi MG, Ozón B, Vera González SM, García-Pardo J, Obregón WD. Plant protease inhibitors as emerging antimicrobial peptide agents: a comprehensive review. Pharmaceutics. 2024;16(5):582. https://doi.org/10.3390/pharmaceutics16050582
  13. 13. Mustafa G, Mehmood R, Mahrosh HS, Mehmood K, Ahmed S. Investigation of plant antimicrobial peptides against selected pathogenic bacterial species using a peptide-protein docking approach. Biomed Res Int. 2022;2022(1):1077814. https://doi.org/10.1155/2022/1077814
  14. 14. Zhang YM, Ye DX, Liu Y, Zhang XY, Zhou YL, Zhang L, et al. Peptides, new tools for plant protection in eco-agriculture. Adv Agrochem. 2023;2(1):58–78. https://doi.org/10.1016/j.aac.2023.01.003
  15. 15. Huang Y, Liu D, Liu Y, Jia Z, Zhao T, Zhao Z, et al. Mechanisms of antifungal activity of ehimensin F6 produced by Paenibacillus ehimensis HD against Penicillium expansum. Int J Food Microbiol. 2025;435:111184. https://doi.org/10.1016/j.ijfoodmicro.2025.111184
  16. 16. Li X, Wang W, Liu S, Ruan C, Yi L, Deng L, et al. Effects of the peptide H-OOWW-NH2 and its derived lipopeptide C12-OOWW-NH2 on controlling of citrus postharvest green mold. Postharvest Biol Technol. 2019;158:110979. https://doi.org/10.1016/j.postharvbio.2019.110979
  17. 17. Li X, Feng G, Wang W, Yi L, Deng L, Zeng K. Effects of peptide C12-OOWW-NH2 on transcriptome and cell wall of the postharvest fungal pathogen Penicillium digitatum. Front Microbiol. 2020;11:574882. https://doi.org/10.3389/fmicb.2020.574882
  18. 18. Ortiz-Rodríguez T, Mendoza-Acosta F, Martínez-Zavala SA, Salcedo-Hernández R, Casados-Vázquez LE, Bideshi DK, et al. Thurincin H is a nonhemolytic bacteriocin of Bacillus thuringiensis with potential for applied use. Probiotics Antimicrob Proteins. 2023;15:955–66. https://doi.org/10.1007/s12602-022-09952-2
  19. 19. Quan W, Liu C, Li A, Xue Y, Liu S. Transcriptomics study of sanxiapeptin against Penicillium digitatum as an antifungal. Sci Technol Food Ind. 2022;43:109–17.
  20. 20. Sampaio de Oliveira KB, Leite ML, Rodrigues GR, Duque HM, da Costa RA, Cunha VA, et al. Strategies for recombinant production of antimicrobial peptides with pharmacological potential. Expert Rev Clin Pharmacol. 2020;13:367–90. https://doi.org/10.1080/17512433.2020.1764347
  21. 21. Cai Z, Wang L, Zhang Q, Yang W, Zhang C, Wang H, et al. Eco-friendly coating engineered with antimicrobial lipopeptides maintains freshness and induces genes expression in anthocyanin biosynthesis of blueberry. Int J Biol Macromol. 2025;306:141590. https://doi.org/10.1016/j.ijbiomac.2025.141590
  22. 22. Rautenbach M, Troskie AM, Vosloo JA. Antifungal peptides: to be or not to be membrane active. Biochimie. 2016;130:132–45. https://doi.org/10.1016/j.biochi.2016.05.013
  23. 23. Sanz JM, Maestro B, Miravalles LO, Antón P, Prieto MA. Péptidos con actividad antimicrobiana y sus usos. 2022. p. 1–28.
  24. 24. Han J, Jyoti MdA, Song HY, Jang WS. Antifungal activity and action mechanism of histatin 5-halocidin hybrid peptides against Candida spp. PLoS One. 2016;11:e0150196. https://doi.org/10.1371/journal.pone.0150196
  25. 25. Schaefer S, Vij R, Sprague JL, Austermeier S, Dinh H, Judzewitsch PR, et al. A synthetic peptide mimic kills Candida albicans and synergistically prevents infection. Nat Commun. 2024;15:6818. https://doi.org/10.1038/s41467-024-50491-x
  26. 26. do Nascimento Dias J, de Souza Silva C, de Araújo AR, Souza JMT, de Holanda Veloso Júnior PH, Cabral WF, et al. Mechanisms of action of antimicrobial peptides ToAP2 and NDBP-5.7 against Candida albicans planktonic and biofilm cells. Sci Rep. 2020;10(1):10327. https://doi.org/10.1038/s41598-020-67041-2
  27. 27. Moretta A, Scieuzo C, Petrone AM, Salvia R, Manniello MD, Franco A, et al. Antimicrobial peptides: a new hope in biomedical and pharmaceutical field. Front Cell Infect Microbiol. 2021;11:668632. https://doi.org/10.3389/fcimb.2021.668632
  28. 28. Aguiar TKB, Neto NAS, Freitas CDT, Silva AFB, Bezerra LP, Malveira EA, et al. Antifungal potential of synthetic peptides against Cryptococcus neoformans: mechanism of action studies reveal synthetic peptides induce membrane-pore formation, DNA degradation and apoptosis. Pharmaceutics. 2022;14(8):1678. https://doi.org/10.3390/pharmaceutics14081678
  29. 29. Fernández de Ullivarri M, Arbulu S, García-Gutiérrez E, Cotter PD. Antifungal peptides as therapeutic agents. Front Cell Infect Microbiol. 2020;10:105. https://doi.org/10.3389/fcimb.2020.00105
  30. 30. Silva JJL, de Miranda MRA, Souza PFN. The multiverse of action: how mechanisms of synthetic antifungal peptides could be employed in the food industry. Curr Opin Food Sci. 2023;54:101089. https://doi.org/10.1016/j.cofs.2023.101089
  31. 31. Zhang D, Shi C, Cong Z, Chen Q, Bi Y, Zhang J, et al. Microbial metabolite inspired β-peptide polymers displaying potent and selective antifungal activity. Adv Sci. 2022;9(14):2104871. https://doi.org/10.1002/advs.202104871
  32. 32. Li J, Hu S, Jian W, Xie C, Yang X. Plant antimicrobial peptides: structures, functions and applications. Botanical Studies. 2021;62(1):5. https://doi.org/10.1186/s40529-021-00312-x
  33. 33. Maximiano MR, Rios TB, Campos ML, Prado GS, Dias SC, Franco OL. Nanoparticles in association with antimicrobial peptides (NanoAMPs) as a promising combination for agriculture development. Front Mol Biosci. 2022;9:890654. https://doi.org/10.3389/fmolb.2022.890654
  34. 34. Shu C, Cui K, Li Q, Cao J, Jiang W. Epsilon-poly-l-lysine (ε-PL) exhibits multifaceted antifungal mechanisms of action that control postharvest Alternaria rot. Int J Food Microbiol. 2021;348:109224. https://doi.org/10.1016/j.ijfoodmicro.2021.109224
  35. 35. Yang X, Wang Y, Jiang H, Song R, Liu Y, Guo H, et al. Antimicrobial peptide CB-M exhibits direct antifungal activity against Botrytis cinerea and induces disease resistance to gray mold in cherry tomato fruit. Postharvest Biol Technol. 2023;196:112184. https://doi.org/10.1016/j.postharvbio.2022.112184
  36. 36. Li T, Li L, Du F, Sun L, Shi J, Long M, et al. Activity and mechanism of action of antifungal peptides from microorganisms: a review. Molecules. 2021;26(11):3438. https://doi.org/10.3390/molecules26113438
  37. 37. Martínez-Culebras PV, Gandía M, Garrigues S, Marcos JF, Manzanares P. Antifungal peptides and proteins to control toxigenic fungi and mycotoxin biosynthesis. Int J Mol Sci. 2021;22(24):13261. https://doi.org/10.3390/ijms222413261
  38. 38. She M, Zhou H, Dong W, Xu Y, Gao L, Gao J, et al. Modular metabolic engineering of Bacillus amyloliquefaciens for high-level production of green biosurfactant iturin A. Appl Microbiol Biotechnol. 2024;108(1):311. https://doi.org/10.1007/s00253-024-13083-9
  39. 39. Lei Y, Lyu A, Pan M, Xu H, Shi Q, Li D. Efficacy and mechanisms of antimicrobial peptide BP15 and its lipopeptides against citrus blue mold. LWT. 2025;217:117378. https://doi.org/10.1016/j.lwt.2025.117378
  40. 40. Islas-Flores IR, Minero-García Y, James AC. Proteínas contra las infecciones de las plantas. Revista Ciencia. 2005;56:64–74.
  41. 41. Lin S, Chen X, Chen H, Cai X, Chen X, Wang S. The bioprospecting of microbial-derived antimicrobial peptides for sustainable agriculture. Engineering. 2023;27:222–33. https://doi.org/10.1016/j.eng.2022.08.011
  42. 42. Olascoaga-Del Angel KS, Sánchez-Evangelista G, Carmona-Navarrete I, Galicia-Sánchez M del C, Gómez-Luna A, Islas-Arollo SJ, et al. Péptidos antimicrobianos, una alternativa prometedora para el tratamiento de enfermedades infecciosas. Gaceta medica de México. 2018;154(6):681–8. https://doi.org/10.1159/000501946
  43. 43. Alem D, Maidana M, Dalla Rizza M. Péptidos antimicrobianos como alternativa al uso de fungicidas químicos. Serie de Difusión. 2014;608.

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