The optimisation of seeding rate is crucial for enhancing the growth, fibre yield and profitability of jute (Corchorus olitorius L.). A field experiment was conducted to assess the effects of varying seeding rates on the growth, yield and economic performance of jute. The experiment followed a randomized complete block design (RCBD) with 6 different seeding rates as treatments. Seeding rate ranged from 1.50 to 6.00 kg ha-1, each mixed with inert material to maintain uniform application. Results demonstrated that seeding rate significantly influenced plant growth, yield and profitability. Increasing the seeding rate from 1.5 to 6.0 kg ha-1 increased plant density but reduced plant height, basal diameter and leaf number due to intensified intra-specific competition for light and nutrients. The highest fibre yield (3.70 t ha-1), net return (100057 BDT) and benefit–cost ratio (BCR = 2.07) were achieved at 2.50 kg ha-1, while higher rates (≥6.0 kg ha-1) resulted in yield and profit decline. Correlation analysis revealed positive associations between fibre yield and both plant height (r = 0.776) and basal diameter (r = 0.771). Principal component analysis (PCA) confirmed these relationships, with the first 2 components explaining 76.6 % of the total variance. Overall, a low seeding rate (2.5–3.0 kg ha-1) ensured optimal plant population, efficient resource use and maximum profitability, with 2.50 kg ha-1 identified as the most economically viable rate for C. olitorius cultivation in southwestern Bangladesh.