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Plant Science Today (PST)

Research Articles

Vol. 13 No. 3 (2026)

Gas chromatography-mass spectrometry profiling and antioxidant evaluation of the wood rotting fungus Ganoderma multipileum Ding Hou collected from Mizoram, Northeast India

DOI
https://doi.org/10.14719/pst.12532
Submitted
31 October 2025
Published
30-08-2026 — Updated on 29-09-2026
Versions

Abstract

Macrofungi, particularly wood-rotting fungi, are increasingly recognised as valuable sources of bioactive compounds with potential therapeutic applications. The present study evaluated the antioxidant activity and volatile metabolite composition of Ganoderma multipileum collected from Mizoram University Campus, Aizawl, Mizoram, India. The antioxidant activity of the methanolic extracts of G. multipileum was evaluated using DPPH and ABTS radical scavenging assays, revealing IC₅₀ values of 564.7 μg/mL and 647.7 μg/mL respectively, indicating moderate radical scavenging activity. The gas chromatography-mass spectrometry (GC-MS) analysis revealed a broad range of chemical constituents, highlighting the chemical richness of the extract. The identified compounds include 3-ethoxy-6-(1-tetrahydropyronoxy-5-methyl-4-hexenyl)-2-cyclohexane, fluvalinate, methyl p-coumarate, nonanoic acid and 2-methyl heptanoic acid. These compounds belong to various structural group, including fatty acids, esters, phenolic derivatives and heterocyclic compounds. The presence of these chemically diverse metabolites indicates that the extract contains multiple bioactive constituents with a broad range of biological activities. Many of these chemical groups are well-known for their pharmacological properties, particularly their antioxidant, antimicrobial and anti-inflammatory activities. The combined antioxidant and chemical profiling reveal the pharmacological potential of G. multipileum as a source of natural bioactive metabolites. These findings provide a foundation for future studies aiming to isolate specific compounds and explore their therapeutic applications in nutraceutical, pharmaceutical and industrial contexts.

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