Research Articles
Vol. 13 No. sp5 (2026): Recent Advances in Agriculture
Effect of organic seed treatments and soaking duration on seedling growth and survival of papaya (Carica papaya L.)
Krishi Vigyan Kendra, Banaskantha -II, Sardarkrushinagar Dantiwada Agricultural University (SDAU), Tharad 385 565, Gujarat, India
Krishi Vigyan Kendra, Sardarkrushinagar Dantiwada Agricultural University (SDAU), Deesa 385 535, Gujarat, India
College of Horticulture, Sardarkrushinagar Dantiwada Agricultural University (SDAU), Jagudan 384 460, Gujarat, India
College of Agriculture, Sardarkrushinagar Dantiwada Agricultural University (SDAU), Tharad 385 565, Gujarat, India
Chimanbhai Patel (C P) College of Agriculture, Sardarkrushinagar Dantiwada Agricultural University (SDAU), S K Nagar 385 506, Gujarat, India
Abstract
Papaya seed germination is often slow and erratic due to mechanical restrictions from the seed coat (gelatinous sarcotesta) and imposed physiological dormancy. Seed priming effectively releases dormancy from seeds by exposing them to specific substances for specific times that trigger metabolic activities associated with germination. Although, organic substances such as Beejamrit and Jeevamrit are now being used in several crops for seed priming. However, their comparative effects on papaya seed germination are not well understood. The purpose of this experiment was to evaluate various organic substances to improve papaya seed germination and seedling growth. The experiment was carried out at Krishi Vigyan Kendra, Sardarkrushinagar Dantiwada Agricultural University (SDAU), Tharad, Gujarat, using plastic pro trays under 50 % green shade net in complete randomized design comprised with two factors (S); organic substance viz., S0 (water soaking), S1 (Beejamrit), S2 (Jeevamrit 10 %), S3 (cow dung extract), S4 (novel organic liquid nutrient 1.0 %) and S5 (15 days old fermented buttermilk) and different seed soaking duration viz., D1 (6 hr), D2 (9 hr) and D3 (12 hr). The results revealed that treatment S1 (Beejamrit) demonstrated the fastest germination (18.28 days), highest germination per cent (71.21), lesser days to 7–8 leaf stage (59.94), higher seedlings height (10.80 cm), longest primary roots (9.58 cm), highest survival of seedling (92.58 %) and survival per cent of papaya seedling (94.54) after 5 days of planting in the field. In case of soaking durations, treatment D3 (12 hr soaking) recorded the earliest germination (17.87 days), exhibited higher germination per cent (69.50), took shortest time (58.77 days) to attained 7–8 leaf stage, maximum seedling height (10.23 cm) and the longest primary roots (8.40 cm). However, the seed soaking duration was found statistically non-significant on survival per cent of the seedlings at planting and after transplanting in the field.
References
- 1. Hofmeyer JDJ. Genetic studies of Carica papaya L. So Africa Dept Agr For Sci Bull. 1938;187.
- 2. Habtemariam S. The chemical and pharmacological basis of papaya (Carica papaya L.) as potential therapy for type-2 diabetes and associated diseases. In: Oboh G, editor. Medicinal foods as potential therapies for type-2 diabetes and associated diseases. Cambridge (MA): Academic Press; 2019. p. 333–63. https://doi.org/10.1016/B978-0-08-102922-0.00011-0
- 3. Hewajulige IGN, Dhekney SA. Papayas. In: Caballero B, Finglas PM, Toldrá F, editors. Encyclopedia of food and health. Amsterdam: Academic Press; 2016. p. 209–12. https://doi.org/10.1016/B978-0-12-384947-2.00517-1
- 4. Singh SP, Rao DVS. Papaya (Carica papaya L.). In: Yahia EM, editor. Postharvest biology and technology of tropical and subtropical fruits: Mangosteen to white sapote. Cambridge: Woodhead Publishing; 2011. p. 86–124, 125–6e. https://doi.org/10.1533/9780857092618.86
- 5. Santana LF, Inada AC, Espirito Santo BL, Filiú WF, Pott A, Alves FM, et al. Nutraceutical potential of Carica papaya in metabolic syndrome. Nutrients. 2019;11(7):1608. https://doi.org/10.3390/nu11071608
- 6. Koul B, Pudhuvai B, Sharma C, Kumar A, Sharma V, Yadav D, et al. Carica papaya L.: A tropical fruit with benefits beyond the tropics. Diversity. 2022;14(8):683. https://doi.org/10.3390/d14080683
- 7. Anonymous. Area and production of horticulture crops for 2024–25 (final estimates). New Delhi: Department of Agriculture and Farmers Welfare.
- 8. Anonymous. Directorate of Horticulture, Agriculture, Farmers Welfare and Cooperation Department. Government of Gujarat.
- 9. Parmar VM, Kanzaria DR, Varu DK, Parsana JS. Economic impact of various farming systems on papaya (Carica papaya L.) cv. GJP 1. Int J Res Agron. 2024;7(9):820–3. https://doi.org/10.33545/2618060X.2024.v7.i9k.1677
- 10. Yahiro M. Effects of seed pre-treatments on the promotion of germination in papaya (C. papaya L.). Mem Fac Agric Kagoshima Univ. 1979;15:49–54.
- 11. Desai A, Trivedi A, Panchal B, Desai V. Improvement of papaya seed germination by different growth regulator and growing media under net house condition. Int J Curr Microbiol Appl Sci. 2017;6(9):828–34. https://doi.org/10.20546/ijcmas.2017.609.102
- 12. Saragih MK, Panataria LR, Sitorus E, Manurung AI. Breaking the dormancy of some varieties of papaya (Carica papaya L.) by soaking in KNO₃ solution. J Agric. 2024;3(2):56. https://doi.org/10.47709/joa.v3i02.3963
- 13. Mishra M, Dewangan RK, Dixit A, Yadav S. A review on seed germination and seedling development of papaya under GA₃ and different media. Int J Adv Biochem Res. 2025;9(SP-9):1069–75. https://doi.org/10.33545/26174693.2025.v9.i9Sn.5639
- 14. Chacko EK, Singh RN. The effect of gibberellic acid on the germination of papaya seeds and subsequent seedling growth. Trop Agric. 1966;43:341–6.
- 15. Lange AH. Effect of the sarcotesta on germination of Carica papaya. Bot Gaz. 1961;122:305–11. https://doi.org/10.1086/336119
- 16. Koornneef M, Bentsink L, Hilhorst H. Seed dormancy and germination. Curr Opin Plant Biol. 2002;5(1):33–6. https://doi.org/10.1016/S1369-5266(01)00219-9
- 17. Deb P, Das A, Ghosh SK, Suresh CP. Improvement of seed germination and seedling growth of papaya (Carica papaya L.) through different pre-sowing seed treatments. Acta Hortic. 2010;851:313–6. https://doi.org/10.17660/ActaHortic.2010.851.48
- 18. Rana G, Deb P, Dowarah B, Sushmitha K. Effect of seed pre-treatment on seed germination and seedling growth of papaya. Int J Curr Microbiol Appl Sci. 2020;9(4):1066–71. https://doi.org/10.20546/ijcmas.2020.904.126
- 19. Abhishek, Topno SE, Joseph AV. Effect of different plant growth regulators on seed germination, seedling growth and establishment of papaya (Carica papaya L.) cv. Taiwan-7. J Adv Biol Biotechnol. 2024;27(7):795–802. https://doi.org/10.9734/jabb/2024/v27i71038
- 20. Xi DK, Yap SL, Kumar NN, Toh CC, Ishikawa K, Hori M. Plasma-assisted priming: Improved germination and seedling performance of papaya. Sains Malaysiana. 2023;52(2):599–611. https://doi.org/10.17576/jsm-2023-5202-21
- 21. Somdutt, Bhadu KB, Rathore RS, Shekhawat PS. Jeevamrit and Panchagavya’s consequences on growth, quality and productivity of organically grown crops: A review. Agric Rev. 2023;44(4):451–9.
- 22. Gowthamchand NJ, Madasseri S, Puttaswamy C, Sowjanya TV. Beejamritha: A potential source of seed treatment in sustainable agriculture. Agrobios News Lett. 2023;22(10):51–2.
- 23. Singh S, Singh AB, Mandal A, Thakur JK, Das A, Rajput PS, et al. Chemical and microbiological characterization of organic supplements and compost used in agriculture. Emerg Life Sci Res. 2023;9(2):234–44. https://doi.org/10.31783/elsr.2023.92234244
- 24. Bhoi R, Panigrahi S, Sagar L. Beejamritha elixir: Nurturing seeds of green gram and cowpea for flourishing germination. Agric Sci Dig. 2025:1–6. https://doi.org/10.18805/ag.D-6124
- 25. Mukherjee S, Sain S, Ali MN, Goswami R, Chakraborty A, Ray K, et al. Microbiological properties of Beejamrit, an ancient Indian traditional knowledge, uncover a dynamic plant beneficial microbial network. World J Microbiol Biotechnol. 2022;38(7):111. https://doi.org/10.1007/s11274-022-03296-3
- 26. Bhattacharjee U, Uppaluri RVS. Development of macro and micro-nutrient rich integrated Jeevamrutha bio-fertilizer systems using rural and commercial precursors. Energy Nexus. 2025;17:100402. https://doi.org/10.1016/j.nexus.2025.100402
- 27. Harish DK, Chaitra GB. Analyzing the cost of production and farmers' perception of Jeevamrut: An insight into an organic farming solution. J Farm Sci. 2024;37(1):92–4. https://doi.org/10.61475/JFS.2024.v37i1.21
- 28. Sreenivasa MN, Nagaraj MN, Bhat SN. Beejamrith: A source for beneficial bacteria. Karnataka J Agric Sci. 2009;22(5):1038–40.
- 29. Kumar A, Kumar R, Kanaujiya PK, Kumar V. Jeevamrit: A natural growth booster for plants. Eco Farming. 2024;4(1):1–3.
- 30. Patel M, Islam S, Glick BR, Choudhary N, Yadav VK, Bagatharia S, et al. Zero budget natural farming components Jeevamrit and Beejamrit augment Spinacia oleracea L. growth by ameliorating the negative impacts of salt and drought stress. Front Microbiol. 2024;15. https://doi.org/10.3389/fmicb.2024.1326390
- 31. Indian Council of Agricultural Research. Generic protocols for natural farming. New Delhi: Indian Council of Agricultural Research; p. 1–138.
- 32. Desai CS, Patel JM, Pawar SL, Usadadia VP, Naik VR, Savani NG. Value added products from banana pseudostem. Navsari: Navsari Agricultural University, Soil and Water Management Research Unit; 2016. p. 55–6.
- 33. Bansal M, Jani G, Gadani M, Mehta P. Beejamrit: An eco-friendly seed biopriming tool for sustainable agriculture. Int J Agric Food Sci. 2025;7(12):776–80. https://doi.org/10.33545/2664844X.2025.v7.i12i.1106
- 34. Panchal D, Gajjar K, Chaudhary M, Chaudhary D, Patel CK, Shukla N, et al. Microbial and metabolite profiling reveal the composition of Beejamrit: A bioformulation for seed treatment in sustainable agriculture. Agriculture. 2026;16(1):133. https://doi.org/10.3390/agriculture16010133
- 35. Rodríguez S, Vargas I, Hijuelo A, Loumeto F, Silva JJ, Pérez J, et al. Analysis of the effect of scarification process on papaya (Carica papaya Lin.) seeds germination. In: Seed dormancy and germination. Intech Open; 2020. p. 1–16. https://doi.org/10.5772/intechopen.88012
- 36. Saleem MS, Sajid M, Ahmed Z, Ahmed S, Ahmed N, Shahid UI Islam M. Effect of seed soaking on seed germination and growth of bitter gourd cultivars. IOSR J Agric Vet Sci. 2014;6(6):7–11. https://doi.org/10.9790/2380-0660711
- 37. Lanjhiyana R, Sahu GD, Panigrahi HK. Economics of papaya seedlings in different pre-sowing seed treatments under net house condition. Pharma Innov J. 2023;12(1):2721–3.
- 38. Baghel K, Sahu GD, Chandrakar Y, Chandel Y. Study on effect of organic pre-sowing treatments on seed germination of papaya (Carica papaya L.) in Chhattisgarh plains. Pharma Innov J. 2022;11(11):742–4.
- 39. Jotava KS, Kanzaria DR, Mishra S. The effect of sarcotesta and time of sowing on seed germination of papaya (Carica papaya L.) cv. GJP-1. J Crop Weed. 2023;19(1):84–7. https://doi.org/10.22271/09746315.2023.v19.i1.1664
- 40. Parmar BR, Patel F, Parmar AB, Pandey AK. Effect of organic compounds on seed germination and seedling growth of jack fruit (Artocarpus heterophyllus Lam.) seed. Pharma Innov J. 2018;7(10):702–4.
- 41. Singh Y, Rajput R, Singh SV, Rai AK, Singh SK. Effect of Beejamrit seed treatment on germination, seed vigour and early growth of chickpea under field conditions. Int J Agric Plant Sci. 2026;8(1):175–81.
- 42. Mohan A, Raj SK, Jacob D, Shalini PP, Aparna S, Meenakshi RP, et al. Enhancing rice seedling growth and vigour through liquid organic manure-based seed priming. Plant Sci. Today. 2025;12(3):1–10.
- 43. Ghosh T, Biswas MK. Evaluation of antibacterial and antifungal activity of cow urine against some seed borne microflora. Int J Curr Microbiol Appl Sci. 2018;7(5):1714–27. https://doi.org/10.20546/ijcmas.2018.705.201
- 44. Fiskey VV, Krishna Reddy MB, Kumar S. Beejamrit: An organic seed treatment solution. Agri Journal World. 2023;3(10):31.
- 45. Naikwade PV. Effect of Bijamrita and other organic liquid treatments on seed germination and seedling growth of legume crops. Online Int Interdiscip Res J. 2019;9(3):59–65.
- 46. Lakhani H. The role of Bijamrut in Indian natural farming: A comprehensive review. New Era Agric Mag. 2024;3(2):10–5.
- 47. Mevada K, Makwana BD. Efficiency of plant nutrient enhancer for sustainable agriculture in diverse agro-ecosystem. J Agric Ecol. 2023;16:99–104. https://doi.org/10.58628/JAE-2316-219
- 48. Patra D, Bharti N. Revitalizing agriculture: Role of cow dung and urine in promoting sustainability. Bhartiya Krishi Anusandhan Patrika. 2022;39(1):32–8.
- 49. Dawle PP, Swamy GSK, Kanamadi VC, Kumar P, Thammaiah N, Shantappa T. Effect of different organics on germination and growth of mango stones. Karnataka J Agric Sci. 2011;24(4):606–7.
- 50. Babu DV, Naik BNS, Reddy KB, Jogarao P, Kumar TS, Swamy AA. Effect of Bijamrita and other organic liquid treatments on seed germination and seedling growth of rajma (Phaseolus vulgaris L.). Plant Arch. 2025;25(Suppl 2):601–5. https://doi.org/10.51470/PLANTARCHIVES.2025.v25.supplement-2.073
- 51. Jasani RR, Patil SJ, Patel MA, Tandel BM, Gohil V. Impact of seed priming and organic spray on the growth of khirni (Manilkara hexandra Roxb.). Plant Arch. 2025;25(2):2853–60. https://doi.org/10.51470/PLANTARCHIVES.2025.v25.no.2.411
- 52. Dhatterwal G, Dalal RPS, Dalal M, Kumar P, Singla A, Richa. Evaluating the effectiveness of organic seed priming formulations on germination and seedling growth in acid lime (Citrus aurantifolia Swingle) cv. Kagzi. Int J Plant Soil Sci. 2024;36(9):491–501. https://doi.org/10.9734/ijpss/2024/v36i94998
- 53. Nagaraja YE. Management of major disease of chilli (Capsicum annuum L.) using organics. Karnataka J Agric Sci. 2009;22(5):1201.
- 54. Gajjar K, Chaudhary M, Patel S, Vasa N, Maniyar R, Agrawal D, et al. Natural farming as a sustainable agricultural approach enhances soil health and microbial diversity in wheat cultivation: A metagenomic perspective. Front Agron. 2026;8:1755662. https://doi.org/10.3389/fagro.2026.1755662
- 55. Champaneri DD, Patel NK, Desai CS, Desai DH. Efficacy of novel organic liquid nutrient and novel plus organic liquid nutrient on quantitative traits of Indian bean [Lablab purpureus (L.) Sweet]. Int J Plant Soil Sci. 2021;33(17):105–15. https://doi.org/10.9734/ijpss/2021/v33i1730555
- 56. Verma AK, Verma L. Role of Indian knowledge systems in reducing chemical dependency in seed treatment. Int J Plant Pathol Microbiol. 2025;5(1):53–7. https://doi.org/10.22271/27893065.2025.v5.i1a.191
- 57. Shahane AA. Overview of natural farming: A new environmentally responsible production system. Acta Sci Agric. 2024;8:25–30. https://doi.org/10.31080/ASAG.2024.08.1360
- 58. Devakumar N, Shubha S, Gouder SB, Rao GGE. Microbial analytical studies of traditional organic preparations beejamrutha and jeevamrutha. In: Rahmann G, Aksoy U, editors. Proceedings of the 4th ISOFAR Scientific Conference: Building Organic Bridges; Istanbul, Turkey. 2014. p. 639–42.
- 59. Behera SR, Bora H, Sharma A. Unlocking seed vitality and soil health with Beejamrit. Agri-India Today. 2026;6(4):23.
- 60. Ravisankar N, Prusty AK, Raghavendra KJ, Velmurugan A, Kumar V, Shamim M, et al. Natural farming research: Status and future strategy. Modipuram (Meerut): ICAR-Indian Institute of Farming Systems Research; 2025. p. 95.
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