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Plant Science Today (PST)

Research Articles

Vol. 13 No. 3 (2026)

First molecular evidence of mixed infection of mungbean yellow mosaic India virus and cowpea mild mottle virus in soybean from India

DOI
https://doi.org/10.14719/pst.12816
Submitted
18 November 2025
Published
13-05-2026 — Updated on 02-07-2026
Versions

Abstract

Soybean (Glycine max L.) is an important oilseed and protein-rich legume crop widely cultivated for food, feed and industrial uses. However, viral diseases pose a major constraint to soybean production, leading to significant yield losses. Accurate molecular identification of viral pathogens is essential for understanding disease epidemiology and developing effective management strategies. During field surveys conducted in 2022–2023 across soybean-growing regions of Assam, North-East India, soybean plants exhibiting mosaic, mottling and leaf deformation symptoms were frequently observed. Symptomatic samples were collected and subjected to molecular detection using coat protein gene-specific primers for mungbean yellow mosaic India virus (MYMIV; genus Begomovirus) and degenerate primers targeting Carlaviruses. Polymerase chain reaction (PCR) amplification revealed the presence of both MYMIV and cowpea mild mottle virus (CPMMV) either as single infections or in mixed infections. Sequence analysis and phylogenetic reconstruction further confirmed the identity of MYMIV and CPMMV isolates associated with symptomatic soybean plants. To our knowledge, this study provides the first molecular evidence of MYMIV and CPMMV co-infection in soybean from Assam, North-East India. The occurrence of mixed infection highlights potential epidemiological implications and underscores the need for continuous virus surveillance and integrated disease management strategies to safeguard soybean production in the region.

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