Skip to main navigation menu Skip to main content Skip to site footer

Research Articles

Vol. 13 No. sp2 (2026): Recent Advances in Agriculture

Impact of seed priming treatments and paclobutrazol applications on growth, flowering and seed yield in Salvia splendens Sellow ex. Schult.

DOI
https://doi.org/10.14719/pst.12936
Submitted
26 November 2025
Published
20-04-2026

Abstract

An investigation was carried out to assess the effect of seed priming treatments and foliar application of paclobutrazol, on growth, flowering and seed yield attributes in “Salvia splendens”. The study was structured using a randomized complete block design (RCBD) with sixteen treatment combinations replicated thrice with plot size of 1.0 × 1.0 m and followed spacing as 30 × 30 cm. Among the seed priming treatments, priming of seed in cow dung slurry for 24 hr significantly improved all the growth, flowering and yield parameters under study. Whereas, among different doses of paclobutrazol, spraying of plants with 10.0 ppm paclobutrazol resulted in the highest values for plant spread (39.04 cm), number of branches per plant (10.78), number of spikes per plant (15.42), number of florets per spike (50.31), number of pods per plant (460.80), seed yield per plant (6.48 g), per plot (58.27 g) except plant height (42.42 cm) and spike length (12.81 cm) which were highest in control. The treatment combination, SPT2 × PD3 i.e. seed priming with cow dung slurry for 24 hr and foliar application of paclobutrazol at 10 ppm, exhibited highest values for plant spread (42.34 cm), number of branches per plant (12.03), number of spikes per plant (16.83), number of florets per spike (60.67), number of pods per plant (546.03), seed yield per plant (7.36 g), per plot (16.83 g). However, the control lot (SPT2 × PD1) revealed maximum values for plant height and spike length as well. Accordingly, it may be inferred from the outcomes of the investigation that seed priming with cow dung slurry for 24 hr along with the foliar application of paclobutrazol at 10.0 ppm is an appropriate treatment combination for promoting the growth, flowering and seed production in salvia.

References

  1. 1. Kandemir N. The morphological, anatomical and karyological properties of endemic Salvia hypargela Fich and Mey. (Lamiaceae) in Turkey. Pak J Bot. 2003;35:219–36.
  2. 2. Khan A, Rehman NU, Alkharfy KM, Gilani AH. Antidiarrheal and antispasmodic activities of Salvia splendens are mediated through activation of K+ channels. Bangladesh J Pharmacol. 2011;6(2):111–6 https://doi.org/10.3329/bjp.v6i2.9156
  3. 3. Hu D, Kawazoe K, Takaishi Y. Diterpenoids from Salvia splendens. Phytochemistry. 1997;46:781–4 https://doi.org/10.1016/S0031-9422(97)00180-5
  4. 4. Ashok AD, Velmurugan S. Review on seed production techniques in flowering ornamentals. J Pharmacogn Phytochem. 2020;9(5):190–8.
  5. 5. Ashraf M, Foolad MR. Pre-sowing seed treatment: a shotgun approach to improve germination, plant growth and crop yield under saline and non-saline conditions. Adv Agron. 2005;88:223–71 https://doi.org/10.1016/S0065-2113(05)88006-X
  6. 6. Paparella S, Araújo SS, Rossi G, Wijayasinghe M, Carbonera D, Balestrazzi A. Seed priming: state of the art and new perspectives. Plant Cell Rep. 2015;34:1281–93 https://doi.org/10.1007/s00299-015-1784-y
  7. 7. Chatterjee N, et al. On-farm seed priming interventions in agronomic crops. Acta Agric Slov. 2018;111:715–35 https://doi.org/10.14720/aas.2018.111.3.19
  8. 8. Zulfiqar F. Effect of seed priming on horticultural crops. Sci Hortic (Amsterdam). 2021;286:110197 https://doi.org/10.1016/j.scienta.2021.110197
  9. 9. Gupta Y, Kaintura P. Response of paclobutrazol as spray and drench on pot-grown fuchsia cv. Pink Galore. J Plant Sci Res. 2017;2:13–7.
  10. 10. Desta B, Amare G. Paclobutrazol as a plant growth regulator. Chem Biol Technol Agric. 2021;8:1–15 https://doi.org/10.1186/s40538-020-00199-z
  11. 11. Kumar S, et al. Paclobutrazol treatment as a potential strategy for higher seed and oil yield in field-grown Camelina sativa L. BMC Res Notes. 2012;5:137 https://doi.org/10.1186/1756-0500-5-137
  12. 12. Panse VG, Sukhatme PV. Statistical Methods for Agricultural Workers. New Delhi: Indian Council of Agricultural Research; 2000. p. 157–65.
  13. 13. Sheoran OP. OPSTAT: Online statistical analysis tool. Hisar: CCS Haryana Agricultural University; 2006.
  14. 14. Rahman SME, Islam MA, Rahman MM, Oh DH. Effect of cattle slurry on growth, biomass yield and chemical composition of maize fodder. Asian-Australas J Anim Sci. 2008;21(11):1592–8 https://doi.org/10.5713/ajas.2008.80190
  15. 15. Jeptoo A, Aguyoh JN, Saidi M. Improving carrot yield and quality through the use of bio-slurry manure. Sustain Agric Res. 2013;2(1):164–72 https://doi.org/10.5539/sar.v2n1p164
  16. 16. Mao L, Gao Y, Yao Y, Qien Y, Tang Y. Dwarfing effects of paclobutrazole, daminozide and chlormequat on potting scarlet sage. Acta Hortic Sin. 1991;18(2):177–9.
  17. 17. Al-Khassawneh NM, Karam NS, Shibli RA. Growth and flowering of black iris (Iris nigricans Dinsm.) following treatment with plant growth regulators. Sci Hortic (Amsterdam). 2006;107(2):187–93 https://doi.org/10.1016/j.scienta.2005.10.003
  18. 18. Keever GJ, Kessler JR. Paclobutrazol effects on bedding plants during production, shipping and the retail environment when applied in production. J Environ Hortic. 2008;26(2):123–7 https://doi.org/10.24266/0738-2898-26.2.123
  19. 19. El-Aal M, Mohamed Y. Effect of pinching and paclobutrazol on growth, flowering, anatomy and chemical composition of potted geranium (Pelargonium zonale L.). Int J Plant Soil Sci. 2017;17(6):1–22 https://doi.org/10.9734/IJPSS/2017/34527
  20. 20. Giradharala RB, Purohit VL. Effect of fresh cow dung slurry soil application on growth and quality parameters of okra (Abelmoschus esculentus L. Moench) fruits. Pharma Innov J. 2022;11(11):1763–7.
  21. 21. Padmini K, Janakiram, Naik LB. Effect of nutrients and growth regulators on seed yield in China aster (Callistephus chinensis) cv. Poornima. Indian J Agric Sci. 2013;83(3):349–51.
  22. 22. Abo-Elhasan JA, Mohamed AT, Darwish MA, Mahgoub MH. Effect of paclobutrazol and growing media on vegetative growth, flowering and total carbohydrates of Callistemon citrinus. Middle East J Agric Res. 2018;7(3):826–35.
  23. 23. Banswal AK, Dilta BS, Sharma BP, Baweja HS, Gupta YC. Effect of bulb sizes, growing substrates and paclobutrazol doses on potted chincherinchee (Ornithogalum thyrsoides Jacq.). Indian J Hortic. 2015;72(2):257–61 https://doi.org/10.5958/0974-0112.2015.00048.1
  24. 24. Chauhan V, Bohra M, Tomar R, Dobhal N. Effect of paclobutrazol and daminozide on floral attributes of chrysanthemum (Dendranthema grandiflora). Int J Curr Microbiol Appl Sci. 2021;10(2):2754–9 https://doi.org/10.20546/ijcmas.2021.1002.304
  25. 25. Tiwari JK, Singh RP. Effect of pre-planting GA₃ treatment on tuberose. J Ornamental Hortic. 2002;5:44–5.
  26. 26. Ramzan F, Younis A, Riaz A, Ali S, Siddique MI, Lim KB. Pre-planting exogenous application of gibberellic acid influences sprouting, vegetative growth, flowering and subsequent bulb characteristics of ‘Ad-Rem’ tulip. Hortic Environ Biotechnol. 2015;55:479–88 https://doi.org/10.1007/s13580-014-0113-7
  27. 27. Pangtu S, Sharma BP, Dilta BS, Chaudhary SVS. Effect of seed priming on growth and flowering of China aster (Callistephus chinensis L.) Nees. Agric Inl. 2018;5(1):38–42 https://doi.org/10.5958/2454-8634.2018.00007.4
  28. 28. Magnitskiy SV. Controlling seedling height by treating seeds with plant growth regulators [dissertation]. Columbus (OH): The Ohio State University; 2004.
  29. 29. Bisht P, et al. Effect of different treatment modules on seed yield and quality in African marigold (Tagetes erecta L.). Int J Plant Soil Sci. 2023;35(14):42–50 https://doi.org/10.9734/ijpss/2023/v35i143019
  30. 30. Ibrahim H, Aniyikaye R, Adewoyin DTE, Osunde AO, Bala A. Effect of different combinations of organic and inorganic nitrogen sources on growth and pod yield of okra (Abelmoschus esculentus L.). Colloquia SSSN. 2020;44:243–8.
  31. 31. Cline MG. Apical dominance. Bot Rev. 1991;57(4):318–58 https://doi.org/10.1007/BF02858771
  32. 32. Leyser O. Regulation of shoot branching by auxin. Trends Plant Sci. 2003;8(11):541–5 https://doi.org/10.1016/j.tplants.2003.09.008
  33. 33. Domagalska MA, Leyser O. Signal integration in the control of shoot branching. Nat Rev Mol Cell Biol. 2011;12(4):211–21 https://doi.org/10.1038/nrm3088
  34. 34. Chauhan V, Bohra M, Tomar R, Dobhal N. Effect of paclobutrazol and daminozide on vegetative attributes of China aster (Callistephus chinensis) cv. Poornima. Indian J Agric Sci. 2020;83(3):349–51.
  35. 35. Wainwright H, Irwin HL. The effects of paclobutrazol and pinching on Antirrhinum flowering pot plants. J Hortic Sci. 1987;62(3):401–4 https://doi.org/10.1080/14620316.1987.11515798
  36. 36. Rademacher W. Growth retardants: effects on gibberellin biosynthesis and other metabolic pathways. Annu Rev Plant Physiol Plant Mol Biol. 2000;51:501–31 https://doi.org/10.1146/annurev.arplant.51.1.501
  37. 37. Ahmed S, et al. Shattering and yield expression of sesame (Sesamum indicum L.) genotypes influenced by paclobutrazol concentration under rainfed conditions of Pothwar. Plant Biol (Stuttg). 2023;23:1–13 https://doi.org/10.1186/s12870-023-04145-7
  38. 38. Setia RC, Kaur P, Setia N, Anuradha. Influence of paclobutrazol on growth and development of fruit in Brassica juncea (L.) Czern and Coss. Plant Growth Regul. 1996;20:307–16 https://doi.org/10.1007/BF00043323
  39. 39. Manjunatha GS, Upper SN, Pujari BT, Yeledahalli NA, Kuligod VB. Effect of farmyard manure treated with jeevamrutha on yield attributes, yield and economics of sunflower (Helianthus annuus L.). Karnataka J Agric Sci. 2009;22:198–9.
  40. 40. Pathania S, Dilta BS, Kumar A. Response of biostimulants on growth, flowering, seed yield and quality of China aster (Callistephus chinensis (L.) Nees). Int J Bioresour Stress Manag. 2023;14(8):1108–15 https://doi.org/10.23910/1.2023.3552
  41. 41. Singh MK, Hemlata P. Effect of foliar application of GA₃ and micro-elements on vigour and flowering behaviour of African marigold (Tagetes erecta) cv. Double African Yellow. J Nat Resour Dev. 2016;11(2):70–3.

Downloads

Download data is not yet available.