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Plant Science Today (PST)

Research Articles

Vol. 13 No. 3 (2026)

An optimised in vitro propagation protocol for scarlet banana (Musa coccinea Andrews) promoting efficient shoot multiplication, rooting and acclimatisation

DOI
https://doi.org/10.14719/pst.13054
Submitted
4 December 2025
Published
05-08-2026 — Updated on 29-09-2026
Versions

Abstract

Scarlet banana (Musa coccinea Andrews) is a valuable ornamental species, but its commercial production remains constrained by low multiplication rates, non-uniform planting material and limited availability of species-specific propagation protocols. This study developed a comprehensive in vitro regeneration protocol for M. coccinea by sequentially optimising basal medium, cytokinin and auxin concentrations, rooting conditions and acclimatisation substrates. To our knowledge, this is the first integrated protocol for M. coccinea that covers shoot initiation, shoot multiplication, root induction and ex vitro acclimatisation in a single regeneration system. Among the basal media tested, Murashige and Skoog (MS) medium produced the highest survival rate (88.88 %), the highest shoot initiation percentage (88.88 %) and the greatest shoot height (4.20 cm). During shoot multiplication, 2.0 mg L-1 6-benzylaminopurine (BAP) produced the highest number of shoots per explant, while the combination of 2.0 mg L-1 BAP and 0.3 mg L-1 α-naphthaleneacetic acid (α-NAA) further enhanced shoot proliferation and produced vigorous, compact shoots. Root induction was most effective on medium supplemented with 0.5 mg L-1 α-NAA, which produced the greatest root number and root length. During acclimatisation, the substrate composed of alluvial soil, burnt rice husk and sand supported the highest survival rate and strongest vegetative growth. The study demonstrated that optimised nutrient composition, hormonal balance and substrate selection are critical for efficient regeneration. The optimised protocol provides a practical and reproducible system for large-scale production of uniform and high-quality M. coccinea plantlets for ornamental horticulture.

References

  1. 1. Dhivya S, Ashutosh S, Gowtham I, Baskar V, Harini AB, Mukunthakumar S, et al. Molecular identification and evolutionary relationships between the subspecies of Musa by DNA barcodes. BMC Genomics. 2020;21(1):659. https://doi.org/10.1186/s12864-020-07036-5
  2. 2. Christelova P, Valarik M, Hribova E, De Langhe E, Dolezel J. A multi-gene sequence-based phylogeny of the Musaceae (banana) family. BMC Evol Biol. 2011;11(1):103. https://doi.org/10.1186/1471-2148-11-103
  3. 3. Inta W, Traiperm P, Ruchisansakun S, Janssens SB, Viboonjun U, Swangpol SC. Evolution and classification of Musaceae based on male floral morphology. Plants. 2023;12(8):1602. https://doi.org/10.3390/plants12081602
  4. 4. Lim TK. Musa coccinea. In: Lim TK, editor. Edible medicinal and non-medicinal plants. Vol. 3, Fruits. Dordrecht: Springer Netherlands; 2012. p. 560–2. https://doi.org/10.1007/978-94-007-2534-8_75
  5. 5. Marta H, Cahyana Y, Djali M, Pramafisi G. The properties, modification and application of banana starch. Polymers. 2022;14(15):3092. https://doi.org/10.3390/polym14153092
  6. 6. Hasnain A, Naqvi SAH, Ayesha SI, Khalid F, Ellahi M, Iqbal S, et al. Plants in vitro propagation with its applications in food, pharmaceuticals and cosmetic industries; current scenario and future approaches. Front Plant Sci. 2022;13:1009395. https://doi.org/10.3389/fpls.2022.1009395
  7. 7. Justine AK, Kaur N, Savita, Pati PK. Biotechnological interventions in banana: current knowledge and future prospects. Heliyon. 2022;8(11):e11636. https://doi.org/10.1016/j.heliyon.2022.e11636
  8. 8. Elyazid DMA, Salama AM, Zanaty A, Abdalla N. In vitro propagation and acclimatization of banana plants: antioxidant enzymes, chemical assessments and genetic stability of regenerates as a response to copper sulphate. Plants. 2021;10(9):1853. https://doi.org/10.3390/plants10091853
  9. 9. Xiang D, Yang X, Liu B, Chu Y, Liu S, Li C. Bio-priming of banana tissue culture plantlets with endophytic Bacillus velezensis EB1 to improve Fusarium wilt resistance. Front Microbiol. 2023;14:1146331. https://doi.org/10.3389/fmicb.2023.1146331
  10. 10. Arshad R, Razzaq T, Ahmad B, Hou T, Li C, Jin Z, et al. Banana breeding by genome design. J Integr Plant Biol. 2025;67(11):2816–47. https://doi.org/10.1111/jipb.70025
  11. 11. Das U, Prasad SS, Sahoo T, Paramanik S, Halder A, S P. Unveiling the potential of banana (Musa spp.) improvement through genetic manipulation: current trends and future implications. Plant Signal Behav. 2026;21(1):2656013. https://doi.org/10.1080/15592324.2026.2656013
  12. 12. Adero M, Tripathi JN, Tripathi L. Advances in somatic embryogenesis of banana. Int J Mol Sci. 2023;24(13):10999. https://doi.org/10.3390/ijms241310999
  13. 13. Babaei N, Abdullah NA, Saleh G, Abdullah TL. An efficient in vitro plantlet regeneration from shoot tip cultures of Curculigo latifolia, a medicinal plant. Sci World J. 2014;2014:275028. https://doi.org/10.1155/2014/275028
  14. 14. Hwang JE, Park HB, Tho JH, Kim M, Park HJ, Kim S, et al. Influence of basal medium and organic additives on in vitro germination and plant growth of endangered orchid Gastrochilus fuscopunctatus. Plants. 2025;14(20):3133. https://doi.org/10.3390/plants14203133
  15. 15. Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant. 1962;15(3):473–97. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  16. 16. White PR. The cultivation of animal and plant cells. BioSci. 1963;4(3):1–13. https://doi.org/10.1093/aibsbulletin/4.3.13-d
  17. 17. Knudson L. A new nutrient solution for the germination of orchid seed. Am Orchid Soc Bull. 1946;15(1):214–7.
  18. 18. Muhammad A, Rashid H, Hussain I, Naqvi SMS. Proliferation-rate effects of BAP and kinetin on banana (Musa spp. AAA Group) ‘Basrai’. HortScience. 2007;42(5):1253–5. https://doi.org/10.21273/HORTSCI.42.5.1253
  19. 19. Sipen P, Davey MR. Effects of N(6)-benzylaminopurine and indole acetic acid on in vitro shoot multiplication, nodule-like meristem proliferation and plant regeneration of Malaysian bananas (Musa spp.). Trop Life Sci Res. 2012;23(2):67–80.
  20. 20. Su YH, Liu YB, Zhang XS. Auxin-cytokinin interaction regulates meristem development. Mol Plant. 2011;4(4):616–25. https://doi.org/10.1093/mp/ssr007
  21. 21. Schaller GE, Bishopp A, Kieber JJ. The yin-yang of hormones: cytokinin and auxin interactions in plant development. Plant Cell. 2015;27(1):44–63. https://doi.org/10.1105/tpc.114.133595
  22. 22. Kurepa J, Shull TE, Smalle JA. Antagonistic activity of auxin and cytokinin in shoot and root organs. Plant Direct. 2019;3(2):e00121. https://doi.org/10.1002/pld3.121
  23. 23. Trunjaruen A, Luecha P, Taratima W, Addi M. The optimization of medium conditions and auxins in the induction of adventitious roots of pokeweed (Phytolacca americana L.) and their phytochemical constituents. Scientifica. 2023;2023:1–14. https://doi.org/10.1155/2023/2983812
  24. 24. Barbez E, Dunser K, Gaidora A, Lendl T, Busch W. Auxin steers root cell expansion via apoplastic pH regulation in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 2017;114(24):E4884–93. https://doi.org/10.1073/pnas.1613499114
  25. 25. Pokharel U, Neelgund G, Ray RL, Balan V, Kumar S. Biochar for soil amendment: applications, benefits and environmental impacts. Bioengineering. 2025;12(11):1137. https://doi.org/10.3390/bioengineering12111137
  26. 26. Abul Soad AA, Markhand GS, Akhtar N. Effect of different substrates on survival of transplanted banana 'Grand Naine' cultivar into greenhouse. Acta Hortic. 2012;928:131–8. https://doi.org/10.17660/ActaHortic.2012.928.14

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