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Plant Science Today (PST)

Research communications

Vol. 13 No. sp2 (2026): Recent Advances in Agriculture

Optimisation of auxin concentration and exposure duration for enhanced adventitious rooting and survival in Nerium oleander L. stem cuttings

DOI
https://doi.org/10.14719/pst.13240
Submitted
17 December 2025
Published
30-04-2026

Abstract

Nerium oleander L. is an important ornamental and medicinal shrub widely propagated through stem cuttings; however, successful rooting and survival are often limited by endogenous physiological constraints. Optimisation of auxin concentration and exposure duration is therefore critical for enhancing adventitious root formation and establishment. The present study evaluated the effects of different concentrations and dipping durations of indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) on sprouting, rooting, root quality and survival of semi-hardwood stem cuttings of N. oleander. The experiment was conducted under controlled polyhouse conditions using a completely randomised design with 15 treatments, including IBA and NAA at 60, 80 and 100 ppm for 12 and 24 hr, along with water-soaked and untreated controls. Observations on days to sprouting, days to rooting, rooting percentage, root length, root number, root diameter and survival percentage were recorded and statistically analysed. Auxin-treated cuttings significantly outperformed controls for all parameters studied. Among the treatments, IBA at 100 ppm for 24 hr resulted in the earliest sprouting (22.1 days) and rooting (26.9 days), highest rooting percentage (98.2 %), superior root length (18.6 cm) and maximum survival (93.7 %). Although NAA treatments enhanced root number, they were comparatively less effective in producing a vigorous and stable root system. The results demonstrate that both auxin concentration and exposure duration play a decisive role in adventitious rooting. Based on overall performance, IBA at 100 ppm for 24 hr is recommended as an optimal protocol for efficient and commercial propagation of N. oleander.

References

  1. 1. Don S, Hamid N, Taha H, Sukri R, Metali F. Vegetative propagation of Hoya imperialis and Hoya coronaria by stem cutting and micropropagation. Tropical Life Sciences Research. 2021;32(3):1–23. https://doi.org/10.21315/tlsr2021.32.3.1
  2. 2. Sabatino L, D’Anna F, Iapichino G. Improved propagation and growing techniques for oleander nursery production. Horticulturae. 2019;5(3):55. https://doi.org/10.3390/horticulturae5030055
  3. 3. Kumar R, Ahmed N, Sharma O, Lal S. Influence of auxins on rooting efficacy in carnation (Dianthus caryophyllus L.) cuttings. Journal of Horticultural Sciences. 2014;9(2):157–60. https://doi.org/10.24154/jhs.v9i2.187
  4. 4. Abdulraahman Y, Ayoub M. Effect of propagation dates, media and auxins treatments on rooting of Monterey cypress (Cupressus macrocarpa) semi-hard wood cuttings. KUJAS. 2023;14(3):190–200. https://doi.org/10.58928/ku23.14321
  5. 5. Hamidon A, Shah R, Razali R, Lob S. Effect of different types and concentration of rooting hormones on Momordica cochinchinensis root vine cuttings. Malaysian Applied Biology. 2020;49(4):127–32. https://doi.org/10.55230/mabjournal.v49i4.1602
  6. 6. Kanimozhi T, Shoba N, Venkatesan K. Effect of plant growth regulators on rooting and sprouting of different stem cuttings of guava (Psidium guajava L.) cv. Lucknow-49 under mist chamber condition. Madras Agricultural Journal. 2018;105:336–40. https://doi.org/10.29321/maj.2018.000154
  7. 7. Simpson B, Maxwell N, McWilliams E. ‘White Cloud’ and ‘Green Cloud’ cenizo. HortScience. 1984;19(1):130–1. https://doi.org/10.21273/hortsci.19.1.130
  8. 8. Smith N, Wareing P. Rooting of hardwood cuttings in relation to bud dormancy and the auxin content of the excised stems. New Phytologist. 1972;71(1):63–80. k
  9. 9. Shrestha J, Bhandari N, Baral S, Marahatta S, Pun U. Effect of rooting hormones and media on vegetative propagation of bougainvillea. Ornamental Horticulture. 2023;29(3):397–406. https://doi.org/10.1590/2447-536x.v29i3.2637
  10. 10. Li S, Huang P, Ding G, Zhou L, Tang P, Sun M, et al. Optimization of hormone combinations for root growth and bud germination in Chinese fir (Cunninghamia lanceolata) clone leaf cuttings. Scientific Reports. 2017;7(1). https://doi.org/10.1038/s41598-017-05295-z
  11. 11. Chen H, Lei Y, Sun J, Ma M, Deng P, Quan J, et al. Effects of different growth hormones on rooting and endogenous hormone content of two Morus alba L. cuttings. Horticulturae. 2023;9(5):552. https://doi.org/10.3390/horticulturae9050552
  12. 12. Rosier C, Frampton J, Goldfarb B, Blazich F, Wise F. Growth stage, auxin type and concentration influence rooting of stem cuttings of Fraser fir. HortScience. 2004;39(6):1397–402. https://doi.org/10.21273/hortsci.39.6.1397
  13. 13. Costa C, Almeida M, Ruedell C, Schwambach J, Maraschin F, Fett-Neto A. When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings. Frontiers in Plant Science. 2013;4. https://doi.org/10.3389/fpls.2013.00133
  14. 14. Li J, Dunn B, Wang Y, Goad C. Responses to propagation substrate and rooting hormone products to facilitate asexual propagation of Silene chalcedonica and Silene coronaria. Journal of Environmental Horticulture. 2016;34(3):80–3. https://doi.org/10.24266/0738-2898-34.3.80
  15. 15. Al-Salem M, Karam N. Auxin, wounding and propagation medium affect rooting response of stem cuttings of Arbutus andrachne. HortScience. 2001;36(5):976–8. https://doi.org/10.21273/hortsci.36.5.976
  16. 16. Jull L, Warren S, Blazich F. Rooting ‘Yoshino’ Cryptomeria stem cuttings as influenced by growth stage, branch order and IBA treatment. HortScience. 1994;29(12):1532–5. https://doi.org/10.21273/hortsci.29.12.1532
  17. 17. Stančevic A, Gavrilovic J, Stanković D, Zwet T. ‘Pitoma slanopadja’, a natural pear hybrid between Pyrus amygdaliformis and Pyrus communis. HortScience. 1984;19(2):254–5. https://doi.org/10.21273/hortsci.19.2.254
  18. 18. An H, Meng J, Xu F, Jiang S, Wang X, ChunHui S, et al. Rooting ability of hardwood cuttings in highbush blueberry (Vaccinium corymbosum L.) using different indole-butyric acid concentrations. HortScience. 2018;54(2):194–9. https://doi.org/10.21273/hortsci13691-18
  19. 19. Tsipouridis C, Thomidis T, Michailides Z. Influence of some external factors on the rooting of peach and nectarine shoot hardwood cuttings. Australian Journal of Experimental Agriculture. 2005;45(1):107. https://doi.org/10.1071/ea03120

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