The present investigation evaluated the interactive effects of biofertilisers and Jeevamrit on the physicochemical and biochemical fruit quality traits of guava (Psidium guajava L.) cv. G-27. A field experiment was conducted over 2 years (2023–24 and 2024–25) at Rajmata Vijayaraje Scindia Krishi Vishwavidyalaya (RVSKVV), Gwalior, Madhya Pradesh, India, using a factorial randomised block design (FRBD) comprising 16 treatment combinations (4 biofertiliser levels × 4 Jeevamrit levels) with 3 replications on 10-year-old guava trees. Biofertiliser treatments consisted of graded doses of Azotobacter, phosphate-solubilising bacteria (PSB) and potassium solubilising bacteria (KSB), applied at 0, 200, 250 and 300 mL tree-1, in combination with Jeevamrit levels of 0, 1.90, 2.50 and 3.125 L tree-1. The integrated treatment T₁₆ (Azotobacter 300 mL tree-1 + PSB 300 mL tree-1 + KSB 300 mL tree-1 + Jeevamrit 3.125 L tree-1), applied at the pea stage, resulted in the highest total soluble solids (11.48 °Brix), ascorbic acid (160.10 mg 100 g-1 pulp) and total sugars (11.39 %), along with enhanced pulp weight (249.91 g) and bioactive compound content, while recording the lowest titratable acidity (0.17 %). These improvements are attributed to microbially mediated nutrient mobilisation through nitrogen, phosphorus and potassium solubilisation, enhanced assimilate partitioning to fruits and the activation of antioxidant metabolism driven by microbial metabolites and phytohormones. The pooled results confirm the effectiveness of integrated biofertiliser-biostimulant application in improving guava fruit quality under sustainable nutrient management.
