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Plant Science Today (PST)

Research Articles

Vol. 13 No. 2 (2026)

Phytochemical characterisation of Parthenium hysterophorus L. extracts and their antioxidant and antimicrobial activities

DOI
https://doi.org/10.14719/pst.13414
Submitted
29 December 2025
Published
14-04-2026 — Updated on 21-04-2026
Versions

Abstract

Parthenium hysterophorus L. is a noxious and highly invasive weed known for its adverse effects on livestock and human health. Despite its negative impact, the plant possesses notable therapeutic potential. This study investigated the phytochemical composition, conducted quantitative analyses and evaluated the antioxidant and antibacterial activities of various P. hysterophorus extracts. The crude extract of methanol and ethyl acetate of different plant parts was prepared using maceration techniques and antibacterial activity of its crude extract through the agar well diffusion method. The methanol leaves extract exhibited the highest phenolic content (20.38 ± 0.02 mg/g GAE [gallic acid equivalent]), followed by the methanol flower extract (18.99 ± 0.02 mg/g GAE). The ethyl acetate flower extract showed the highest flavonoid content (123.98 ± 5.7 mg/g QE [quercetin equivalent]), while the methanol stems extract contained the most alkaloids (566 ± 6.14 mg/g AAE [ascorbic acid equivalent]). In terms of antioxidant activity, the methanol flower extract demonstrated greater DPPH radical scavenging capacity (IC₅₀ = 482.99 µg/mL) compared to the ethyl acetate leaves extract (IC₅₀ = 637.63 µg/mL). The strongest ABTS scavenging activity was observed in the ethyl acetate leaf extract (IC₅₀ = 79.04 µg/mL) and the methanol flower extract exhibited superior ferric reducing antioxidant power. Antibacterial testing revealed the highest activity against Microbacterium sp. (26.33 ± 0.57 mm at 60 mg/mL) in the ethyl acetate leaf extract. The extract effectiveness against all tested bacterial strains ranked as follows: methanol flower > ethyl acetate leaf > methanol leaf > methanol root > ethyl acetate root > methanol stem. The Ultraviolet–Visible (UV-Vis) spectroscopy and Fourier transform infrared (FTIR) spectroscopy analyses further confirmed the presence of key bioactive compounds. These findings highlight the potential of P. hysterophorus as a source of natural antioxidant and antimicrobial agents, which may serve as alternatives to synthetic compounds.

References

  1. 1. Jamshidi-Kia F, Lorigooini Z, Amini-Khoei H. Medicinal plants: past history and future perspective. Journal of herbmed pharmacology. 2017;7(1):1–7. https://doi.org/10.15171/jhp.2018.01
  2. 2. Zahra W, Rai SN, Birla H, Singh SS, Rathore AS, Dilnashin H, et al. Economic importance of medicinal plants in Asian countries. In: Bioeconomy for sustainable development. Singapore: Springer Singapore; 2019. p. 359–77. https://doi.org/10.1007/978-981-13-9431-7_19
  3. 3. Gupta PD, Birdi TJ. Development of botanicals to combat antibiotic resistance. Journal of Ayurveda and integrative medicine. 2017;8(4):266–75. https://doi.org/10.1016/j.jaim.2017.05.004
  4. 4. Kumar S, Chashoo G, Saxena AK, Pandey AK. Parthenium hysterophorus: a probable source of anticancer, antioxidant and anti‐HIV agents. BioMed Research International. 2013;1:81073. https://doi.org/10.1155/2013/810734
  5. 5. Rajak BK, Rani P, Mandal P, Chhokar RS, Singh N, Singh DV. Emerging possibilities in the advancement of herbicides to combat acetyl-CoA carboxylase inhibitor resistance. Frontiers in Agronomy. 2023;20(5):1218824. https://doi.org/10.3389/fagro.2023.1218824
  6. 6. Hernández-Marín DA, Castro-Rios R, Chávez-Montes A, Castillo-Hernández SL, Elizondo-Luevano JH, Muñoz-Ortega MH, et al. Antiparasitic activity of isolated fractions from Parthenium incanum Kunth against the hemoflagellate protozoan Trypanosoma cruzi. Antibiotics. 2024;13(7):622. https://doi.org/10.3390/antibiotics13070622
  7. 7. Ahmad J, Bagheri R, Bashir H, Baig MA, Al-Huqail A, Ibrahim MM, et al. Organ-specific phytochemical profiling and antioxidant analysis of Parthenium hysterophorus L. BioMed research international. 2018;2018:9535232. https://doi.org/10.1155/2018/9535232
  8. 8. Singh P, Haldhar P, Das T, Govil P, Chaubey G, Gupta MK, et al. Thermal stress-induced metabolic shifts in the Parthenium beetle: an untargeted metabolomics study. Archives of Insect Biochemistry and Physiology. 2026;121(1):e70126. https://doi.org/10.1002/arch.70126
  9. 9. Choudhary H, Saket A, Singh S, Srinivasan T, Shukla R. In silico assessment of 4-methoxypelargonidin and luteolinidin from Parthenium hysterophorous as acetylcholinesterase inhibitors of Phlebotomus papatasi. Scientific Reports. 2026;1:33591. https://doi.org/10.1038/s41598-025-33591-6
  10. 10. Singh A, Singh N, Singh S, Srivastava RP, Singh L, Verma PC, et al. The industrially important genus Kaempferia: an ethnopharmacological review. Frontiers in Pharmacology. 2023;27(1):1099523. https://doi.org/10.3389/fphar.2023.1099523
  11. 11. Srivastava RP, Kumar S, Singh L, Madhukar M, Singh N, Saxena G, et al. Major phenolic compounds, antioxidant, antimicrobial and cytotoxic activities of Selinum carvifolia (L.) collected from different altitudes in India. Frontiers in Nutrition. 2023;13(10):1180225. https://doi.org/10.3389/fnut.2023.1180225
  12. 12. Banerjee S, Mukherjee A, Dhak D, Madhogaria B, Chakraborty S, Kundu A, et al. Facile biosynthesis of iron oxide nanoparticles from Parthenium hysterophorus extract and investigating their bioactive and photocatalytic applications. 3 Biotech. 2025;15(12):422. https://doi.org/10.1007/s13205-025-04581-2
  13. 13. Dixit P, Singh N, Singh L, Srivastava RP, Pandey S, Singh S, et al. Screening for the biochemical profile and biological activity in Cephalotaxus and Taxus collected from north-eastern Himalayas. ACS Agricultural Science & Technology. 2023;3(8):694–700. https://doi.org/10.1021/acsagscitech.3c00126
  14. 14. Izhar SK, Bano N, Rizvi SF, Gupta A, Pandey P, Lakhanpal S, et al. Actinobacteria mediated synthesis of silver nanoparticles and their weedicidal potential against Parthenium hysterophorous Linnaeus. Scientific Reports. 2025;15(1):31540. https://doi.org/10.1038/s41598-025-15657-7
  15. 15. Al-Rimawi F, Imtara H, Khalid M, Salah Z, Parvez MK, Saleh A, et al. Assessment of antimicrobial, anticancer and antioxidant activity of Verthimia iphionoides plant extract. Processes. 2022;10(11). https://doi.org/10.3390/pr10112375
  16. 16. Dubale S, Kebebe D, Zeynudin A, Abdissa N, Suleman S. Phytochemical screening and antimicrobial activity evaluation of selected medicinal plants in Ethiopia. Journal of Experimental Pharmacology. 2023;15:51–62. https://doi.org/10.2147/JEP.S379805
  17. 17. Grygorieva O, Vergun O, Klymenko S, Zhurba M, Sedláčková VH, Ivanišova E, et al. Estimation of phenolic compounds content and antioxidant activity of leaves extracts of some selected non-traditional plants. Potravinarstvo Slovak Journal of Food Sciences. 2020;14(7):501–9. https://doi.org/10.5219/1314
  18. 18. Ralte L, Khiangte L, Thangjam NM, Kumar A, Singh YT. GC–MS and molecular docking analyses of phytochemicals from the underutilized plant, Parkia timoriana revealed candidate anti-cancerous and anti-inflammatory agents. Scientific Reports. 2022;12(1):1–21. https://doi.org/10.1038/s41598-022-07320-2
  19. 19. Neupane P, Lamichhane J. Estimation of total phenolic content, total flavonoid content and antioxidant capacities of five medicinal plants from Nepal. Vegetos. 2020;33(2):360–6. https://doi.org/10.1007/s42535-020-00116-7
  20. 20. Madan H, Gogia S, Sharma S. Antimicrobial and spermicidal activities of Parthenium hysterophorus Linn. and Alstonia scholaris Linn. Indian Journal of Natural Products and Resources. 2011;2(4):458–63
  21. 21. Singh N, Mansoori A, Jiwani G, Solanke AU, Thakur TK, Kumar R, et al. Antioxidant and antimicrobial study of Schefflera vinosa leaves crude extracts against rice pathogens. Arabian Journal of Chemistry. 2021;14(7):103243. https://doi.org/10.1016/j.arabjc.2021.103243
  22. 22. Dinda B, Dinda S, DasSharma S, Banik R, Chakraborty A, Dinda M. Therapeutic potentials of baicalin and its aglycone, baicalein against inflammatory disorders. European Journal of Medicinal Chemistry. 2017;131:68–80. https://doi.org/10.1016/j.ejmech.2017.03.004
  23. 23. Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S, et al. Effect of extraction solvent on total phenol content, total flavonoid content and antioxidant activity of Limnophila aromatica. Journal of food and drug analysis. 2014;22(3):296–302. https://doi.org/10.1016/j.jfda.2013.11.001
  24. 24. Singh N, Singh N, Kumar J. Genetic and genome engineering of underutilized fruit and vegetable crops: challenges and opportunities for postharvest quality improvement. Bioengineered Fruit and Vegetables. 2024;22:237–52. https://doi.org/10.1201/9781003501954-11
  25. 25. Ali B, Arshad A, Javed MA, Kaplan A, Suleman F, Hafeez A, et al. Biochar-induced regulation on primary and secondary metabolites in plants under abiotic stress. In: Biochar in mitigating abiotic stress in plants. Academic Press; 2025. p. 119–33. https://doi.org/10.1016/B978-0-443-24137-6.00007-0
  26. 26. Malik C, Dwivedi S, Rabuma T, Kumar R, Singh N, Kumar A, et al. De novo sequencing, assembly and characterization of Asparagus racemosus transcriptome and analysis of expression profile of genes involved in the flavonoid biosynthesis pathway. Frontiers in Genetics. 2023;14:1236517. https://doi.org/10.3389/fgene.2023.1236517
  27. 27. Sahariah P, Bora J, Malik S, Syiem D, Bhan S, Hussain A, et al. Therapeutic potential of Dillenia indica L. in attenuating hyperglycemia-induced oxidative stress and apoptosis in alloxan-administered diabetic mice. Frontiers in Bioscience-Landmark. 2023;28(5):105. https://doi.org/10.31083/j.fbl2805105
  28. 28. Dakal TC, Singh N, Kaur A, Dhillon PK, Bhatankar J, Meena R, et al. New horizons in microbial fuel cell technology: applications, challenges and prospects. Biotechnology for Biofuels and Bioproducts. 2025;18(1):79. https://doi.org/10.1186/s13068-025-02649-y
  29. 29. Poojary MM, Vishnumurthy KA, Adhikari AV. Extraction, characterization and biological studies of phytochemicals from Mammea suriga. Journal of pharmaceutical analysis. 2015;5(3):182–9. https://doi.org/10.1016/j.jpha.2015.01.002
  30. 30. Khameneh B, Iranshahy M, Ghandadi M, Ghoochi Atashbeyk D, Fazly Bazzaz BS, Iranshahi M. Investigation of the antibacterial activity and efflux pump inhibitory effect of co-loaded piperine and gentamicin nanoliposomes in methicillin-resistant Staphylococcus aureus. Drug development and industrial pharmacy. 2015;41(6):989–94. https://doi.org/10.3109/03639045.2014.920025
  31. 31. Datta S, Saxena DB. Pesticidal properties of parthenin (from Parthenium hysterophorus) and related compounds. Pest Management Science: formerly Pesticide Science. 2001;57(1):95–101. https://doi.org/10.1002/1526-4998(200101)57:1<95::AID-PS248>3.0.CO;2-J
  32. 32. de Carvalho LM, Casali VW, de Souza MA, de Almeida Barbosa LC, Cecon PR. Crescimento, teor de partenolídeo e de prolina em plantas de Tanacetum parthenium (L.) Schultz-Bip crescidas em substrato com diferentes teores de umidade. Acta Scientiarum. Biological Sciences. 2005;27(1):151–7. https://doi.org/10.4025/actasciagron.v27i1.2137
  33. 33. Majdi M, Karimzadeh G, Malboobi MA, Omidbaigi R, Mirzaghaderi G. Induction of tetraploidy to feverfew (Tanacetum parthenium Schulz-Bip.): morphological, physiological, cytological and phytochemical changes. HortScience. 2010;45(1):16–21. https://doi.org/10.21273/HORTSCI.45.1.16
  34. 34. Serrano J, Puupponen-Pimiä R, Dauer A, Aura AM, Saura-Calixto F. Tannins: current knowledge of food sources, intake, bioavailability and biological effects. Molecular nutrition & food research. 2009;53(S2):S310–29. https://doi.org/10.1002/mnfr.200900039
  35. 35. Singh N, Jiwani G, Rocha LS, Mazaheri R. Bioagents and volatile organic compounds: an emerging control measures for rice bacterial diseases. In: Bacterial diseases of rice and their management. Apple Academic Press; 2023. p. 255–74. https://doi.org/10.1201/9781003331629-16
  36. 36. Sahu N, Dubey A, Singh N, Arya KR, Yadav P, Chaturvedi P, et al. Steroidal alkaloids from Sarcococca saligna (Buxaceae): in vitro and in silico evaluation of their cytotoxic potential. ACS omega. 2025;10(41):48111–29. https://doi.org/10.1021/acsomega.5c04143
  37. 37. Singh N, Mansoori A, Jiwani G, Solanke AU, Kumar R, Kumar A. Evaluation of antioxidant and antimicrobial potential of Thespesia lampas root extracts. Journal of experimental biology and agricultural sciences. 2021;9(1):87–99. https://doi.org/10.18006/2021.9(1).87.99
  38. 38. Kumar BA, Lakshman K, Nandeesh R, Kumar PA, Manoj B, Kumar V, et al. In vitro alpha-amylase inhibition and in vivo antioxidant potential of Amaranthus spinosus in alloxan-induced oxidative stress in diabetic rats. Saudi Journal of Biological Sciences. 2011;18(1):1–5. https://doi.org/10.1016/j.sjbs.2010.08.002
  39. 39. Sahu N, Singh N, Arya KR, Reddy SS, Rai AK, Shukla V, et al. Assessment of the dual role of Lyonia ovalifolia (Wall.) Drude in inhibiting AGEs and enhancing GLUT4 translocation through LC-ESI-QTOF-MS/MS determination and in silico studies. Frontiers in Pharmacology. 2023;28(14):1073327. https://doi.org/10.3389/fphar.2023.1073327
  40. 40. Singh N, Pandey R, Chandraker SK, Pandey S, Malik S, Patel D. Use of wild edible plants can meet the needs of future generation. In: Agro-biodiversity and agri-ecosystem management. Singapore: Springer Nature Singapore; 2022. p. 341–66. https://doi.org/10.1007/978-981-19-0928-3_18
  41. 41. Hussain A, Khan AA, Ali M, Zamani GY, Iqbal Z, Ullah Q, et al. In-vitro and in-vivo assessment of toxic effects of Parthenium hysterophorus leaves extract. Journal of the Chilean Chemical Society. 2022;67(2):5484–9. https://doi.org/10.4067/S0717-97072022000205484
  42. 42. Wang Y, Zhang S, Ma Y, Du X, Zong Q, Lin D, et al. Solvent effects on terpenoid compositions and antioxidant activities of Cinnamomum camphora (L.) J. Presl extracts and the main antioxidant agent evaluation through in vitro and in vivo assay. Chemical and Biological Technologies in Agriculture. 2024;11(1):2. https://doi.org/10.1186/s40538-023-00524-2
  43. 43. Shalaby EA, Shanab SM. Antioxidant compounds, assays of determination and mode of action. African journal of pharmacy and pharmacology. 2013;7(10):528–39. https://doi.org/10.5897/AJPP2013.3474
  44. 44. Gonzalez-Burgos E, Gomez-Serranillos MP. Terpene compounds in nature: a review of their potential antioxidant activity. Current medicinal chemistry. 2012;19(31):5319–41. https://doi.org/10.2174/092986712803833335
  45. 45. Mamudu U, Santos JH, Umoren SA, Alnarabiji MS, Lim RC. Investigations of corrosion inhibition of ethanolic extract of Dillenia suffruticosa leaves as a green corrosion inhibitor of mild steel in hydrochloric acid medium. Corrosion Communications. 2024;15:52–62. https://doi.org/10.1016/j.corcom.2023.10.005
  46. 46. Gottardi D, Bukvicki D, Prasad S, Tyagi AK. Beneficial effects of spices in food preservation and safety. Frontiers in microbiology. 2016;7:186557. https://doi.org/10.3389/fmicb.2016.01394
  47. 47. Supritha P, Radha KV. Estimation of phenolic compounds present in the plant extracts using high pressure liquid chromatography, antioxidant properties and its antibacterial activity. Indian Journal of Pharmaceutical Education and Research. 2018;52(2):321–6
  48. 48. Mishra A, Sharma AK, Kumar S, Saxena AK, Pandey AK. Bauhinia variegata leaf extracts exhibit considerable antibacterial, antioxidant and anticancer activities. BioMed Research International. 2013;1:915436. https://doi.org/10.1155/2013/915436
  49. 49. Thakur A, Singh S, Dulta K, Singh N, Ali B, Hafeez A, et al. Nutritional evaluation, phytochemical makeup, antibacterial and antioxidant properties of wild plants utilized as food by the Gaddis, a tribal tribe in the Western Himalayas. Frontiers in Agronomy. 2022;19(4):1010309. https://doi.org/10.3389/fagro.2022.1010309
  50. 50. Chintada V, Golla N. Exploring the therapeutic potential of bioactive compounds from plant sources. In: Biotechnological intervention in production of bioactive compounds: biosynthesis, characterization and applications. Cham: Springer Nature Switzerland; 2025. p. 229–47. https://doi.org/10.1007/978-3-031-76859-0_15

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