Skip to main navigation menu Skip to main content Skip to site footer

Research Articles

Vol. 13 No. sp2 (2026): Recent Advances in Agriculture

Role of nano urea plus and nano DAP for the improvement of nitrogen use efficiency in rice

DOI
https://doi.org/10.14719/pst.13715
Submitted
17 January 2026
Published
05-05-2026

Abstract

Efficient nitrogen (N) management is vital for sustainable rice production, given the heavy reliance on urea and substantial environmental losses associated with its use. This study evaluated the integrated use of nano urea plus [n-UP; 20 % N (w/v)] and nano DAP [n-DAP; 8 % N (w/v) and 16 % P (w/v)] to improve growth, physiology, biochemical, yield and nitrogen use efficiency (NUE) of rice under a reduced recommended dose of nitrogen (RDN). A field experiment was conducted during rabi 2023 using the rice variety ADT 53 in a randomised block design (RBD) with 8 treatments and 3 replications. The results revealed that 75 % RDN along with foliar applications of n-UP at the active tillering (AT) stage and n-DAP at the panicle initiation (PI) stage significantly enhanced physiological traits such as photosynthetic rate (35.5 µmol CO₂ m-2 s-1), stomatal conductance (0.568 mol H₂O m-2 s-1), Fv/Fm ratio (0.836) and biochemical traits such as soluble protein (SP) (24.1 mg g-1) and nitrate reductase (NR) activity (135.5 µg NO₂- g-1 h-1). This treatment also recorded the highest grain yield of 6.3 t ha-1, grain N (0.75 %), straw N (1.44 %) and improved NUE indices, including N uptake efficiency (41.0), AE (25.9), physiological efficiency (32.1) and NUE(yield-based) (17.7). Correlation and cluster analyses revealed strong positive associations between NUE indices and yield, demonstrating that 75 % RDN with nanofertilisers was equally effective as 100 % RDN. The study confirms that staggered foliar application of n-UP and n-DAP can achieve 25 % fertiliser savings while sustaining yield and enhancing NUE.

References

  1. 1. Khatibi SMH, Adviento-Borbe MA, Dimaano NG, Radanielson AM, Ali J. Advanced technologies for reducing greenhouse gas emissions from rice fields: is hybrid rice the game changer? Plant Commun. 2025;6(2):101224. https://doi.org/10.1016/j.xplc.2024.101224
  2. 2. Unified Portal for Agricultural Statistics (UPAg). All India: crop-wise area, production and yield – rice. Government of India; 2025.
  3. 3. United States Department of Agriculture (USDA). World agricultural production: rice production estimates for 2024–2025. Foreign Agricultural Service; 2025.
  4. 4. Ministry of Chemicals and Fertilizers. Fertilizers scenario in the country: Department of Fertilizers ensures timely and adequate fertilizer availability through scientific planning, robust production growth and subsidy support. Press Information Bureau; 2025.
  5. 5. Govindasamy P, Muthusamy SK, Bagavathiannan M, Mowrer J, Jagannadham PTK, Maity A, et al. Nitrogen use efficiency: a key to enhance crop productivity under a changing climate. Front Plant Sci. 2023;14:1121073. https://doi.org/10.3389/fpls.2023.1121073
  6. 6. Thirunagari BK, Kancheti M, Kumar R, Kota SH. Managing synthetic nitrogen fertilizer emissions in India: insights from field surveys across 102 districts. J Environ Manage. 2024;366:121909. https://doi.org/10.1016/j.jenvman.2024.121909
  7. 7. Alam MS, Khanam M, Rahman MM. Environment-friendly nitrogen management practices in wetland paddy cultivation. Front Sustain Food Syst. 2023;7:1020570. https://doi.org/10.3389/fsufs.2023.1020570
  8. 8. Liu Y, Fu Z, Wang W, Zhang J, Cao Q, Tian Y, et al. Effects of nitrogen management optimization practices on rice productivity and nitrogen loss: a meta-analysis. Front Plant Sci. 2025;16:1485144. https://doi.org/10.3389/fpls.2025.1485144
  9. 9. Mim JJ, Rahman SM, Khan F, Paul D, Sikder S, Das HP, et al. Towards smart agriculture through nano-fertilizer: a review. Mater Today Sustain. 2025;30:101100. https://doi.org/10.1016/j.mtsust.2025.101100
  10. 10. Frank M, Husted S. Is India’s largest fertilizer manufacturer misleading farmers and society using dubious plant and soil science? Plant Soil. 2024;496:257–67. https://doi.org/10.1007/s11104-023-06191-4
  11. 11. Sikka R, Kalia A, Ahuja R, Sidhu SK, Chaitra P. Substitution of soil urea fertilization to foliar nano urea fertilization decreases growth and yield of rice and wheat. Plant Soil. 2025;512(1):1475–91. https://doi.org/10.1007/s11104-024-07157-w
  12. 12. Ranjan S, Sow S, Sadhu S, Sahoo R, Nath D, Samanta D, et al. Unlocking higher productivity and nutrient use efficiency in cereals through nano-fertilizers. Crop Des. 2025;4(4):100119. https://doi.org/10.1016/j.cropd.2025.100119
  13. 13. Kumar A, Sheoran P, Devi S, Kumar N, Malik K, Rani M, et al. Strategic switching from conventional urea to nano-urea for sustaining the rice–wheat cropping system. Plants. 2024;13(24):3523. https://doi.org/10.3390/plants13243523
  14. 14. Singh RK, Upadhyay PK, Hasanain M, Shekhawat K, Rathore SS, Meena VS, et al. Nano-urea driven improvements in nitrogen efficiency and wheat productivity: trade-offs, synergies, and climate resilience pathways. Front Sustain Food Syst. 2026;9:1670081. https://doi.org/10.3389/fsufs.2025.1670081
  15. 15. Sahoo BR, Dash AK, Mohapatra KK, Mohanty S, Sahu SG, Sahoo BR, et al. Strategic management of nano-fertilizers for sustainable rice yield, grain quality and soil health. Front Environ Sci. 2024;12:1420505. https://doi.org/10.3389/fenvs.2024.1420505
  16. 16. Upadhyay PK, Dey A, Singh VK, Dwivedi BS, Singh T, GA R, et al. Conjoint application of nano-urea with conventional fertilizers: an energy-efficient and environmentally robust approach for sustainable crop production. PLoS One. 2023;18(7):e0284009. https://doi.org/10.1371/journal.pone.0284009
  17. 17. Gopinath KA, Visha Kumari V, Singh VK, Shanker AK, Reddy KS, Kundu S, et al. Combined application of nano-DAP and conventional fertilizers improved agronomic and economic performance of rainfed maize, nutrient and energy use efficiency. Front Agron. 2025;7:1723814. https://doi.org/10.3389/fagro.2025.1723814
  18. 18. Kumar A, Sheoran P, Kumar N, Devi S, Kumar A, Malik K, et al. Elucidating morphogenic and physiological traits of rice with nitrogen substitution through nano-nitrogen under salt stress conditions. BMC Plant Biol. 2024;24(1):908. https://doi.org/10.1186/s12870-024-05569-5
  19. 19. Reddy KS, Shivay YS, Kumar D, Pooniya V, Prasanna R, Shrivastava M, et al. Relative performance of urea and nano-urea in conjunction with zinc fertilization on growth, productivity and nitrogen use efficiency in spring wheat. J Soil Sci Plant Nutr. 2024;24(2):3593–609. https://doi.org/10.1007/s42729-024-01780-3
  20. 20. Reddy KS, Shivay YS, Kumar D, Parida BK, Bora R, Borate RB, et al. Nano DAP augments productivity, phosphorus use efficiency and profitability of spring wheat. Sci Rep. 2025;15(1):24771. https://doi.org/10.1038/s41598-025-92364-3
  21. 21. Prince Gondwal MK, Rana MK, Dhankar A, Sahil J, Mohan J. Role of nano-urea in optimizing nitrogen use efficiency, yield and quality of rice: a review. Int J Res Agron. 2024;7(10S):182–7. https://doi.org/10.33545/2618060X.2024.v7.i10Sc.1724
  22. 22. Maloth A, Thatikunta R, Parida BK, Saida Naik D, Gopala Varma NR. Evaluation of nano-DAP on plant growth, enzymatic activity and yield in paddy (Oryza sativa L.). Int J Environ Clim Chang. 2024;14(1):890–7. https://doi.org/10.9734/ijecc/2024/v14i13907
  23. 23. IFFCO-Nanoventions Private Limited. Nano fertilizers; 2025. https://www.nanoventions.in/
  24. 24. Djanaguiraman M, Priyanka AS, Haripriya S, Kalarani MK, Umapathi M. Nanoselenium improves drought tolerance of sorghum via reduced transpiration rate and osmolytic accumulation. S Afr J Bot. 2024;172:93–108. https://doi.org/10.1016/j.sajb.2024.07.012
  25. 25. Yoshida S, Forno DA, Cock JH. Laboratory manual for physiological studies of rice. Los Baños, Philippines: International Rice Research Institute; 1971.
  26. 26. Nicholas JC, Harper JE, Hageman RH. Nitrate reductase activity in soybeans (Glycine max): Effects of light and temperature. Plant Physiol. 1976;58(6):731–5. https://doi.org/10.1104/pp.58.6.731
  27. 27. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265–75. https://doi.org/10.1016/S0021-9258(19)52451-6
  28. 28. Nelson DW, Sommers LE. Determination of total nitrogen in plant material. Agron J. 1973;65(1):109–12. https://doi.org/10.2134/agronj1973.00021962006500010033x
  29. 29. Srikanth B, Subrahmanyam D, Sanjeeva Rao D, Narender Reddy S, Supriya K, Raghuveer Rao P, et al. Promising physiological traits associated with nitrogen use efficiency in rice under reduced nitrogen application. Front Plant Sci. 2023;14:1268739. https://doi.org/10.3389/fpls.2023.1268739
  30. 30. Xiong D. Perspectives of improving rice photosynthesis for higher grain yield. Crop Environ. 2024;3(3):123–37. https://doi.org/10.1016/j.crope.2024.04.001
  31. 31. Verma KK, Song XP, Degu HD, Guo DJ, Joshi A, Huang HR, et al. Recent advances in nitrogen and nano-nitrogen fertilizers for sustainable crop production: A mini-review. Chem Biol Technol Agric. 2023;10(1):111. https://doi.org/10.1186/s40538-023-00488-3
  32. 32. Ding YG, Zhang XB, Quan MA, Li FJ, Tao RR, Zhu M, et al. Tiller fertility is critical for improving grain yield, photosynthesis and nitrogen efficiency in wheat. J Integr Agric. 2023;22(7):2054–66. https://doi.org/10.1016/j.jia.2022.10.005
  33. 33. Liu Y, Gao J, Zhao Y, Fu Y, Yan B, Wan X, et al. Effects of different phosphorus and potassium supply on the root architecture, phosphorus and potassium uptake, and utilization efficiency of hydroponic rice. Sci Rep. 2024;14(1):21178. https://doi.org/10.1038/s41598-024-72287-1
  34. 34. Khandare VP, Gaikwad GK, Ugile SK, Dhembare NP, Labade PD. Effect of foliar application of nano urea, nano DAP and growth regulator on growth, yield and quality of chickpea (Cicer arietinum L.) under inceptisol. Int J Res Agron. 2024;7(12):714–20. https://doi.org/10.33545/2618060X.2024.v7.i12i.2249
  35. 35. Farooq MS, Majeed A, Ghazy AH, Fatima H, Uzair M, Ahmed S, et al. Partial replacement of inorganic fertilizer with organic inputs for enhanced nitrogen use efficiency, grain yield and decreased nitrogen losses under rice-based systems of mid-latitudes. BMC Plant Biol. 2024;24(1):919. https://doi.org/10.1186/s12870-024-05629-w
  36. 36. Kumagai E, Araki A, Kubota F. Correlation of chlorophyll meter readings with gas exchange and chlorophyll fluorescence in flag leaves of rice (Oryza sativa L.). Plant Prod Sci. 2009;12(1):50–3. https://doi.org/10.1626/pps.12.50
  37. 37. Dey A, Jangir N, Verma D, Shekhawat RS, Yadav P, Sadhukhan A. Foliar application of nano urea enhances vegetative growth of Arabidopsis thaliana over equimolar bulk urea through higher induction of biosynthesis genes but suppression of nitrogen uptake and senescence genes. Plant Growth Regul. 2025;105:833–59. https://doi.org/10.1007/s10725-025-01314-6
  38. 38. Sweety RRB, Boominathan P, Senthil A, Jegadeeswari D, Kumar GP. Nanodiammonium phosphate enhances grain yield by modulating gas exchange traits and nutrient use efficiency in rice. Plant Sci Today. 2024;11(sp4):1–10. https://doi.org/10.14719/pst.5508
  39. 39. Keerthana S, Rajavel M, Senthil A, Prasanthrajan M, Sivakumar V. Physiological evaluation of nano DAP on growth and yield of tomato. Plant Sci Today. 2024;11(sp4):1–10. https://doi.org/10.14719/pst.5596
  40. 40. Miranda-Villagomez E, Trejo-Tellez LI, Gomez-Merino FC, Sandoval-Villa M, Sánchez-García P, Aguilar-Méndez MÁ. Nanophosphorus fertilizer stimulates growth and photosynthetic activity and improves phosphorus status in rice. J Nanomater. 2019;2019:5368027. https://doi.org/10.1155/2019/5368027
  41. 41. Feng W, Xue W, Zhao Z, Shi Z, Wang W, Bai Y, et al. Nitrogen fertilizer application rate affects the dynamic metabolism of nitrogen and carbohydrates in kernels of waxy maize. Front Plant Sci. 2024;15:1416397. https://doi.org/10.3389/fpls.2024.1416397
  42. 42. Saitheja V, Prasad V. Performance evaluation of summer irrigated green gram (Vigna radiata L.) by combined application of basal nitrogen and foliar fertilization of nano urea. Plant Sci Today. 2025;12(2):7520. https://doi.org/10.14719/pst.7520
  43. 43. Xudong G, Fengju Z, Teng W, Xiaowei X, Xiaohui J, Xing X. Effects of nitrogen and phosphorus addition on growth and leaf nitrogen metabolism of alfalfa in alkaline soil in Yinchuan Plain of Hetao Basin. PeerJ. 2022;10:e13261. https://doi.org/10.7717/peerj.13261
  44. 44. Kekeli MA, Wang Q, Rui Y. The role of nano-fertilizers in sustainable agriculture: boosting crop yields and enhancing quality. Plants. 2025;14(4):554. https://doi.org/10.3390/plants14040554
  45. 45. Xu T, Zhang H, Gong J, Wang L, Wang Y, Qiu W, et al. Optimizing nitrogen fertilizer rate and investigating mechanism driving grain yield increase for rice in the middle reaches of the Yangtze River. Plants. 2025;14(15):2326. https://doi.org/10.3390/plants14152326
  46. 46. Singh PP, Priyam A, Singh J, Gupta N. Biologically synthesised urea-based nanomaterial shows enhanced agronomic benefits in maize and rice crops during Kharif season. Sci Hortic. 2023;315:111988. https://doi.org/10.1016/j.scienta.2023.111988

Downloads

Download data is not yet available.