Differential proteomic responses of Mimosa pudica L. and Senna auriculata L. Roxb. under ultraviolet-B (UV-B) radiation were examined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional gel electrophoresis (2-DE). Under normal conditions, both species exhibited stable and comparable protein expression patterns. However, UV-B exposure induced distinct, species-specific proteomic alterations. Senna auriculata showed an early and sustained upregulation of stress-responsive proteins, indicating rapid activation of defence and protective mechanisms. In contrast, M. pudica displayed a delayed proteomic response, with changes becoming evident only at later stages of exposure. Both species exhibited a reduction in low molecular weight proteins, suggesting a metabolic shift from growth-related processes to stress adaptation. Overall, the findings highlight that S. auriculata possesses a more efficient and timely proteomic response to UV-B stress than M. pudica, providing valuable insights into the molecular basis of plant resilience under environmental stress conditions.
